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    Wyświetlanie 1-72 z 72
    Tytuł:
    Occurrence of Giardia intestinalis in hospitalized children and in children from orphanages
    Autorzy:
    Okulewicz, J.
    Lucinska, A.
    Galary, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/840193.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Prospect Giardia Test
    infection
    Giardia
    child
    orphanage
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First record of Giardia assemblage D infection in farmed raccoon dogs (Nyctereutes procyonoides)
    Autorzy:
    Solarczyk, Piotr
    Majewska, Anna C.
    Jędrzejewski, Szymon
    Górecki, Marcin T.
    Nowicki, Sławomir
    Przysiecki, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/989740.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    giardia
    molecular genotyping
    raccoon dog
    Opis:
    The presence of Giardia genotypes was investigated in 18 raccoon dogs (Nyctereutes procyonoides) and 80 red foxes (Vulpes vulpes) on one farm. To demonstrate Giardia cysts, fresh and trichrome stained smears were microscopically screened. Two molecular markers were used for Giardia genotyping: a fragment of the beta-giardin gene and a fragment of the glutamate dehydrogenase gene. All faecal samples obtained from red foxes were negative. Giardia cysts were identified only in 2 of the 18 raccoon dogs. The result of genotyping and phylogenetic analysis showed that the G. duodenalis from both raccoon dogs belonged to the D assemblage. This finding of a new animal reservoir of G. duodenalis canids-specific genotypes is important in order to eliminate the risk of infecting other animals bred for fur. Further molecular analyses of Giardia isolates in raccoon dogs are required. The present study represents the first contribution to knowledge of G. duodenalis genotypes in raccoon dogs.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2016, 23, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wodnopochodne epidemie Giardia
    Autorzy:
    Majewska, A C
    Kasprzak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/841170.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    lamblia
    Giardia
    epidemie
    parazytologia
    pasozyty
    epidemiologia
    Opis:
    The authors present the actual informations on waterborne outbreaks of giardiosis as well as the evidences of Giardia cyst transmission by municipal water supply. In the article a recommendation is given to molecular biology techniques which contributed to the armentarium applied in the modern diagnostics of giardiosis.
    Źródło:
    Annals of Parasitology; 1995, 41, 1; 25-31
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    First Molecular Detection of Giardia duodenalis Assemblage B in a Free-Living European Wildcat (Felis s. silvestris) from Luxembourg
    Autorzy:
    Solarczyk, Piotr
    Osten-Sacken, Natalia
    Frantz, Alain C.
    Schneider, Simone
    Pir, Jacques B.
    Heddergott, Mike
    Powiązania:
    https://bibliotekanauki.pl/articles/52071478.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Giardia
    molecular diagnosis
    LAMP
    PCR
    epidemiology
    zoonosis
    Opis:
    Giardia duodenalis is one of the most widespread intestinal parasites of humans and other vertebrates. In terms of public health, identification of Giardia assemblages in wildlife is important because only some assemblages of G. duodenalis can infect humans. Here, we use loop-mediated isothermal amplification (LAMP) and genotyping of analysis of the β-giardin gene to screen the zoonotic assemblages of G. duodenalis recovered from faeces of free-living European wildcats (Felis s. silvestris) from Luxembourg. Giardia DNA was detected in one animal (10%) and assigned to assemblage B by both methods. This is the first detection and genotyping of G. duodenalis in a European wild felid in general, and of assemblage B in particular. Free-living wildcats may act as reservoirs of G. duodenalis infectious for humans and other wildlife and domestic animals. Using a combination of LAMP- and genotyping-based methods allowed effective, sensitive, and rapid detection of a zoonotic G. duodenalis assemblage B in wildlife.
    Źródło:
    Acta Protozoologica; 2019, 58, 1; 1-5
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Giardia duodenalis and Cryptosporidium spp. infection in veterinarians in Poland
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Zwoliński, J.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081928.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Giardia duodenalis
    Cryptosporidium spp.
    veterinarians
    Polska
    Opis:
    The main aim of the study was to determine the risk of two selected zoonosis infections caused by Giardia duodenalis and Cryptosporidium spp. among an occupational group of veterinarians in Poland. Two hundred and ninety-seven samples of stool were tested for the presence of Giardia cysts and Cryptosporidium oocysts using Direct Fluorescent Assay (DFA). There were no positive results for Cryptosporidium. The presence of Giardia cysts was found in two samples of faeces (0.67%). The risk with regard to the parasites Giardia duodenalis and Cryptosporidium spp. seems to be low among the group of veterinarians.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 732-733
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wodnopochodne epidemie Giardia
    WATERBORNE OUTBREAKS OF GIARDIA
    Autorzy:
    Majewska, A. C.
    Kasprzak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2151382.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    lamblia
    Giardia
    epidemie
    parazytologia
    pasozyty
    epidemiologia
    Opis:
    The authors present the actual informations on waterborne outbreaks of giardiosis as well as the evidences of Giardia cyst transmission by municipal water supply. In the article a recommendation is given to molecular biology techniques which contributed to the armentarium applied in the modern diagnostics of giardiosis.
    Źródło:
    Wiadomości Parazytologiczne; 1995, 41, 1; 25-31
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence and multilocus genotyping of Giardia from animals at the zoo of Poznan, Poland
    Autorzy:
    Solarczyk, P.
    Majewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143383.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    prevalence
    genotyping
    Giardia
    animal
    Polska
    zoo animal
    Poznan Zoological Garden
    zoonotic transmission
    Giardia duodenalis
    cyst
    feces sample
    genotype
    identification
    Opis:
    In this study total of 266 fecal samples from 242 animals belonging to 113 species kept in the Poznan Zoological Garden were examined for Giardia. The cysts of Giardia were found only in five samples of feces collected from a giant toad (Bufo marinus), tamandua (Tamandua tetradactyla) and three individuals of cactus mouse (Peromyscus eremicus). Fragments of β-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes were successfully amplified only from the Giardia isolate obtained from the tamandua. Sequence and phylogenetic analyses showed that the Giardia isolate from the tamandua belonged to the B assemblage and showed homologies of 99% to 100% at bg, gdh and tpi loci of the same markers of parasites isolated from humans and animals in various parts of the world. This is the first molecular characterization of G. duodenalis from tamandua.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 3; 169-173
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bialka wydzielnicze Giardia duodenalis - charakterystyka i rola w biologii pasozyta
    Autorzy:
    Zysiak, D
    Powiązania:
    https://bibliotekanauki.pl/articles/840144.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    bialka sekrecyjne
    parazytologia
    funkcje biologiczne
    pasozyty
    Giardia duodenalis
    Opis:
    The article presents the current knowledge on the proteines under question. The first analysed E-S products released by G. duodenalis was a polydisperse hydrophobic complex (16.5-225 kDa), protease VI sensitive, chloroform-methanol insoluble. Based on inhibition studies cysteine protease and metalloprotease were detected in the complex. The further analysis revealed that a 58 kDa heat stable as wall as protease sensitive glycoprotein secreted by G. duodenalis trophozoites is highly immunogenic for the hosts. Before encystation, G. duodenalis trophozoites secrete leucine-rich cyst wall proteins: CWP1, CWP2 and CWP3. Steady-state levels of CWPs gene transcripts are low in non-encysting trophozoites but increase more than 100-fold during encystation. Another protein, which expression also increases during encystation is gGSP (Giardia Granule-Specific Protein). The protein possesses typical strucutre of calcium-binding proteins. Inhibition of gGSP expression abolishes cyst wall formation, suggesting that this protein regulates Ca²⁺-dependent degranulation of encystation-specific secretory vesicles (ESVs ) during cyst wall formation.
    Źródło:
    Annals of Parasitology; 2005, 51, 1; 15-19
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An evaluation of the diagnostic value of ELISA tests from Alexon Inc. and Dialab GmbH in the detection of Giardia lamblia infection
    Autorzy:
    Zurek, K.
    Pietrzyk, A.
    Andrzejak, A.
    Wojtacha, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/840575.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    Giardia lamblia
    infection
    giardiasis
    ELISA test
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Woda jako potencjalne źródło zarażenia ludzi i zwierząt pierwotniakami z rodzajów Cryptosporidium i Giardia
    Water as a potential source of human and animal infection with protozoa of the genera Cryptosporidium and Giardia
    Autorzy:
    Bojar, H.
    Klapec, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/941325.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    pierwotniaki cryptosporidium spp
    giardia spp.
    woda
    skażenie
    Opis:
    Pierwotniaki z rodzaju Cryptosporidium i Giardia to pasożyty przewodu pokarmowego ludzi i zwierząt. Są kosmopolityczne, występują we wszystkich krajach świata. Odgrywają istotną rolę w rozprzestrzenianiu się chorób przenoszonych przez wodę. Pierwotniaki te przedostają się do wody na skutek jej zanieczyszczenia odchodami ludzkimi i zwierzęcymi, ściekami, osadami ściekowymi. Monitorowanie źródeł zanieczyszczenia wody pozwala zapobiegać wodnopochodnym epidemiom dotyczących wody pitnej oraz zbiorników wodnych wykorzystywanych do celów rekreacyjnych. W Polsce badania nad występowaniem Cryptosporidium spp. i Giardia spp. w środowisku prowadzone są od dwudziestu lat. Wysokie skażenie Cryptosporydium stwierdza się w wodach powierzchniowych (83%), niższy odsetek prób pozytywnych stwierdza się w wodzie wstępnie uzdatnionej (13%) i kranowej (22%). W odniesieniu do Giardia zanieczyszczenie wód powierzchniowych wynosi 57% i jest znacznie niższe niż w przypadku Cryptosporidium. Natomiast w przypadku wody wstępnie uzdatnionej i kranowej jest identyczne jak w przypadku Cryptosporidium. Zarażenia pasożytniczymi pierwotniakami są często problemem niedocenianym przez lekarzy. Niedobory w ogólnie dostępnych informacjach, trudności w interpretacji wyników badań, brak dostatecznych standardów diagnostycznych powodują obniżone zainteresowanie tą grupą patogenów, które odgrywają istotną rolę w rozprzestrzenianiu chorób przenoszonych przez wodę.
    Źródło:
    Medycyna Ogólna i Nauki o Zdrowiu; 2011, 17(46), 1; 46-51
    2083-4543
    Pojawia się w:
    Medycyna Ogólna i Nauki o Zdrowiu
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena skuteczności procesów oczyszczania wód powierzchniowych ujmowanych w województwie podkarpackim na podstawie występowania pierwotniaków z rodzaju Cryptosporidium i Giardia oraz klostridiów redukujących siarczyny
    Assessment of effectiveness of surface water treatment processes in Podkarpackie region based on protozoa, Cryptosporidium and Giardia sp., and sulfite-reducing clostridia occurrence
    Autorzy:
    Matuszewska, R.
    Szczotko, M.
    Giziński, R.
    Krogulska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/236386.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
    Tematy:
    parasites
    Cryptosporidium oocysts
    Giardia cysts
    drinking water
    water treatment
    pasożyty
    oocysty Cryptosporidium
    cysty Giardia
    woda przeznaczona do spożycia
    oczyszczanie wody
    Opis:
    According to Polish Ministry of Health regulations, in case Clostridium perfringens spores are identified in treated water samples, Cryptosporidium and Giardia protozoan parasites detection should be part of drinking water quality supervision procedure, both for surface and mixed water intake sites. The study assessed effectiveness in removing of protozoan parasite cysts and oocysts as well as sulfite-reducing clostridia in surface water treatment processes in Podkarpackie region waterworks. Presence of Cryptosporidium sp. oocysts was detected in 83% of surface intake water samples but the contamination was low. The number of`protozoa was a mean of 0.06 oocysts in 1 dm3. Giardia sp. cysts were detected in all raw water samples and their number was a mean of 0.18 cysts in 1 dm3. Spores of sulfite-reducing clostridia were also detected in all water samples (>102 cfu/100 cm3). Parasitic protozoa were not present in samples of treated (coagulation/filtration) and disinfected water. This study demonstrated that the water treatment technologies applied in Podkarpackie region waterworks constitute an effective barrier against protozoan parasites, but are not always sufficient against spores of anaerobic Clostridium sp. present in the raw water.
    Pierwotniaki pasożytnicze z rodzaju Cryptosporidium i Giardia, zgodnie z rozporządzeniem Ministra Zdrowia w sprawie jakości wody przeznaczonej do spożycia przez ludzi, powinny być elementem kontroli jakości wody pochodzącej z ujęć powierzchniowych lub mieszanych, w przypadku gdy w wodzie oczyszczonej zostanie wykryta obecność bakterii Clostridium perfringens. W pracy dokonano oceny skuteczności usuwania cyst i oocyst pierwotniaków pasożytniczych oraz klostridiów redukujących siarczyny w procesach oczyszczania wody powierzchniowej w wodociągach woj. podkarpackiego. Obecność oocyst Cryptosporidium sp. wykryto w 83% pobranych próbek ujmowanej wody powierzchniowej, przy czym jej zanieczyszczenie było nieznaczne. Liczba tych pierwotniaków wynosiła średnio 0,06 oocyst w 1 dm3 wody. Giardia sp. były wykrywane we wszystkich próbkach ujmowanej wody, średnio 0,18 cyst w 1 dm3. Również we wszystkich próbkach wody wykryto obecność przetrwalników klostridiów redukujących siarczyny (>102 jtk/100 cm3). W próbkach wody oczyszczonej (po koagulacji/filtracji) oraz po dezynfekcji nie wykryto obecności pierwotniaków pasożytniczych. Badania wykazały, że technologie oczyszczania wody zastosowane w zakładach wodociągowych województwa podkarpackiego stanowią skuteczną barierę zabezpieczającą wodę przeznaczoną do spożycia przed pierwotniakami pasożytniczymi, ale nie zawsze wystarczającą wobec obecnych w ujmowanej wodzie przetrwalników beztlenowców z rodzaju Clostridium.
    Źródło:
    Ochrona Środowiska; 2013, 35, 3; 49-52
    1230-6169
    Pojawia się w:
    Ochrona Środowiska
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of internal parasites in hamsters in pet shops in Wroclaw
    Autorzy:
    Polozowski, A.
    Piasecki, T.
    Kowalska, M.
    Klimiuk, M.
    Hormanska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/5890.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    prevalence
    parasite
    Giardia
    Giardia duodenalis
    animal infection
    Trichosomoides nasalis
    Syphacia
    Hymenolepis nana
    internal parasite
    hamster
    pet shop
    Wroclaw city
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence and multilocus genotyping of Giardia from animals at the zoo of Poznan, Poland
    Autorzy:
    Solarczyk, P.
    Majewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/840502.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    prevalence
    genotyping
    Giardia
    animal
    Polska
    zoo animal
    Poznan Zoological Garden
    zoonotic transmission
    Giardia duodenalis
    cyst
    feces sample
    genotype
    identification
    Źródło:
    Annals of Parasitology; 2011, 57, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Azithromycin - new drug in giardiasis treatment
    Autorzy:
    Jeske, J.
    Bergiel, A.
    Kamerys, J.
    Ochecka-Szymanska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/839759.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    Giardia intestinalis
    new drug
    giardiasis
    drug
    azithromycin
    parasitosis
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Beta-2-microglobulin and neopterin in patients with giardiasis
    Autorzy:
    Czarnowski, D.
    Miegoc, H.
    Kalinowska, A.
    Prokopowicz, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/840868.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    T lymphocyte
    neopterin
    Giardia
    giardiasis
    beta-2-microglobulin
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tinidazole in treatment of Giardia duodenalis infections in dogs
    Autorzy:
    Polozowski, A.
    Jelonek, N.
    Cekiera, A.
    Piekarska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/6334.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    tinidazole
    treatment
    veterinary medicine
    Giardia duodenalis
    animal infection
    dog
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New Primers for Fast Detection of Giardia duodenalis Assemblages A and B Using Real-time PCR
    Autorzy:
    Solarczyk, Piotr
    Wojtkowiak-Giera, Agnieszka
    Hołysz, Marcin
    Słodkowicz-Kowalska, Anna
    Jagodziński, Paweł P.
    Stojecki, Krzysztof
    Rocka, Anna
    Majewska, Anna C.
    Skrzypczak, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/52160005.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Giardia duodenalis
    real-time PCR
    specific primers
    genotyping
    zoonoses
    Opis:
    Giardia duodenalisis one of the six Giardia species and itis the most common, cosmopolitan flagellate that infects humans and many species of animals. This species exhibits considerable genetic diversity; to date, eight assemblages (A–H) have been defined. These assemblages differ in host specificity: assemblages A and B have been found in both humans and in many animal species. Mixed infections with Giardia (A and B) assemblages have been reported in humans and in animals. Many molecular techniques are effective and rapid for the detection of G. duodenalis and also for the determination of genetic variability of isolates in clinical and environmental samples. In this context, the aim of this study was to design new assemblage-specific primers for rapid detection and identification of G. duodenalis assemblages A and B and both of these assemblages simultaneously using quantitative real-time polymerase chain reaction (qPCR). Fragments of glutamate dehydrogenase and triose phosphate isomerase were used as targets in the design of primers. In conclusion, the use of G. duodenalis assemblage-specific primers designed in this study allows quick identification of human infectious G. duodenalis assemblages A and B as well as mixed AB assemblages in a sample without further sequencing of the amplification products, which reduces the cost of study and the waiting time for the results.
    Źródło:
    Acta Protozoologica; 2018, 57, 1; 43-48
    0065-1583
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Białka wydzielnicze Giardia duodenalis - charakterystyka i rola w biologii pasożyta
    Autorzy:
    Zysiak, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146360.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    bialka sekrecyjne
    parazytologia
    funkcje biologiczne
    pasozyty
    Giardia duodenalis
    Opis:
    The article presents the current knowledge on the proteines under question. The first analysed E-S products released by G. duodenalis was a polydisperse hydrophobic complex (16.5-225 kDa), protease VI sensitive, chloroform-methanol insoluble. Based on inhibition studies cysteine protease and metalloprotease were detected in the complex. The further analysis revealed that a 58 kDa heat stable as wall as protease sensitive glycoprotein secreted by G. duodenalis trophozoites is highly immunogenic for the hosts. Before encystation, G. duodenalis trophozoites secrete leucine-rich cyst wall proteins: CWP1, CWP2 and CWP3. Steady-state levels of CWPs gene transcripts are low in non-encysting trophozoites but increase more than 100-fold during encystation. Another protein, which expression also increases during encystation is gGSP (Giardia Granule-Specific Protein). The protein possesses typical strucutre of calcium-binding proteins. Inhibition of gGSP expression abolishes cyst wall formation, suggesting that this protein regulates Ca²⁺-dependent degranulation of encystation-specific secretory vesicles (ESVs ) during cyst wall formation.
    Źródło:
    Wiadomości Parazytologiczne; 2005, 51, 1; 15-19
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie pierwotniaków z rodzaju Cryptosporidium i Giardia w wodzie powierzchniowej ujmowanej przez wybrane zakłady wodociągowe
    Occurrence of Cryptosporidium and Giardia in the surface water intakes of selected waterworks
    Autorzy:
    Matuszewska, R.
    Szczotko, M.
    Bartosik, M.
    Krogulska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/237391.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
    Tematy:
    woda powierzchniowa
    pierwotniaki pasożytnicze
    oocysty Cryptosporidium
    cysty Giardia
    oczyszczanie wody
    filtracja pospieszna
    surface water
    parasite protozoa
    Cryptosporidium oocysts
    Giardia cysts
    water treatment
    filtration
    Opis:
    Pierwotniaki pasożytnicze z rodzaju Cryptosporidium i Giardia, obok bakterii wskaźnikowych, takich jak E. coli, bakterie grupy coli, paciorkowce kałowe i beztlenowce z rodzaju Clostridium redukujące siarczyny, są istotnym elementem kontroli jakości wody przeznaczonej do spożycia. W pracy dokonano oceny występowania Giardia i Cryptosporidium w wodzie powierzchniowej ujmowanej przez zakłady wodociągowe w Warszawie, Płocku i Białymstoku. Próbki do badań pobrano równolegle z ujęć oraz przed i po procesie filtracji pospiesznej. Określono też liczbę bakterii wskaźnikowych i spor klostridiów redukujących siarczyny. Wykazano, że zanieczyszczenie wody na ujęciach oocytami Cryptosporidium sp. było stosunkowo nieduże, średnio 0,23 w 1 dm3, w przeciwieństwie do liczby cyst Giardia sp., średnio 1,1 w 1 dm3. We wszystkich próbkach wody stwierdzono obecność paciorkowców kałowych i spor klostridiów redukujących siarczyny, natomiast bakterie grupy coli i E. coli oznaczono jedynie w części próbek. Jednocześnie badania wskazały, że technologia oczyszczania wody stosowana w analizowanych zakładach wodociągowych, w tym filtracja pospieszna, stanowiła skuteczną barierę zabezpieczającą wodę przed pierwotniakami pasożytniczymi oraz sporami klostridiów redukujących siarczyny.
    Parasite protozoa of the genera Cryptosporidium and Giardia play an equally significant part in controlling the quality of the water intended for human consumption as do such indicator organisms as Escherichia coli, coliform bacteria, fecal streptococci and anaerobic sulfite-reducing clostridia. The aim of this study was to assess the occurrence of Cryptosporidium and Giardia in the surface water taken in by the waterworks of Warsaw, Plock and Bialystok. Water was sampled simultaneously at the intake, before rapid filtration and after rapid filtration. Simultaneously were also determined the counts of indicator bacteria and those of the sulfite-reducing clostridia spores. It has been demonstrated that water contamination with Cryptosporidium sp. oocysts was relatively low (0.23 per 1 dm3 on average) in contrast to the contamination by Giardia sp. cysts (1.1 per 1 dm3). Fecal streptococci and spores of sulfite-reducing clostridia were detected in all of the water samples, whereas the presence of coliform bacteria and E. coli was determined only in some of them. The results of the study have revealed that the treatment trains used in the investigated waterworks, and specifically the rapid filtration processes, provide a kind of 'safety fence' protecting against parasite protozoa and spores of sulfite-reducing clostridia.
    Źródło:
    Ochrona Środowiska; 2011, 33, 3; 67-69
    1230-6169
    Pojawia się w:
    Ochrona Środowiska
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular identification of Giardia duodenalis isolates from domestic dogs and cats in Wroclaw, Poland
    Autorzy:
    Piekarska, Jolanta
    Bajzert, Joanna
    Gorczykowski, Michał
    Kantyka, Magdalena
    Podkowik, Magdalena
    Powiązania:
    https://bibliotekanauki.pl/articles/990993.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    giardia duodenalis
    assemblage
    dogs
    cats
    nested-pcr
    pcr-rflp
    zoonosis
    Opis:
    Introduction. Giardia duodenalis (G. intestinalis) is a common protozoan causing gastrointestinal disorders in many species of mammals. The genus of Giardia has high molecular diversity. Dogs and cats, in addition to their typical infection with assemblages C, D and F, may be a reservoir of zoonotic assemblages (A and B). Objective. The aim of this study was a genetic characteristic of Giardia isolates of dogs and cats from the area of Wroclaw (Poland). Materials and method. A total of 128 and 33 faecal samples from dogs and cats, respectively, were analyzed by routine coprological methods. The animals were diagnosed on the presence of G. duodenalis antigens in faeces soluble with the use of SNAP Giardia (IDEXX Laboratories) immunosorbent assay. 27 DNA isolates of Giardia were subjected to molecular identification (PCR-RFLP). Results and conclusions. The prevalence of G. duodenalis was 21.1% (27/128) in dogs and 15.1% (5/33) in cats. In dogs, C assemblage was present in 18 (81%) positive stool samples, D assemblage in 2 (9%) samples, B assemblage present in one (4.5%), and mixed assemblages (C and D) occurred in one (4.5%) sample. F assemblage was found in 4 (80%) cats’ positive stool samples and A assemblage occurred in one case (20%). Confirmation of the presence of A and B zoonotic assemblages suggests that infected pets can be a threat to human health. This study describes for the first time the presence of mixed infections within host-specific C and D assemblages in dogs in Poland.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2016, 23, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Taxonomy of Giardia and Cryptosporidium: facts and controversies
    Autorzy:
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/6079.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    taxonomy
    Giardia
    Cryptosporidium
    parasite
    protozoan parasite
    human disease
    parasitic disease
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genotypowanie izolatow Giardia duodenalis uzyskanych od ludzi w zachodnio-centralnej Polsce
    Genotype analysis of Giardia duodenalis isolated obtained from humans in West-central Poland
    Autorzy:
    Solarczyk, P
    Werner, A.
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/838606.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Giardia duodenalis
    genotypowanie
    genotyp
    pasozyty jelitowe
    pasozyty czlowieka
    Polska Srodkowo-Zachodnia
    Źródło:
    Annals of Parasitology; 2010, 56, 2; 171-177
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genotypowanie izolatów Giardia duodenalis uzyskanych od ludzi w zachodnio-centralnej Polsce
    Genotype analysis of Giardia duodenalis isolated obtained from humans in West-central Poland
    Autorzy:
    Solarczyk, P.
    Werner, A.
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143436.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Giardia duodenalis
    genotypowanie
    genotyp
    pasozyty jelitowe
    pasozyty czlowieka
    Polska Srodkowo-Zachodnia
    Opis:
    Giardia duodenalis (syn. G. intestinalis, G. lamblia) is a cosmopolitan flagellate organism belonging to the most common intestinal protozoan parasites of humans and animals. Great genetic heterogeneity has been found within G. duodenalis, where only genotypes representing assemblages A and B have zoonotic potential. Fecal samples (447 specimens) obtained from 232 humans in West-central region of Poland were examined by microscopy and PCR. The total prevalence of Giardia in humans was 1.3%. DNA was extracted from three positive fecal samples and PCR products were obtained after amplification using the β-giardin primers G7 and G759. Sequence and phylogenetic analyses showed that G. duodenalis isolates from humans belonged to A and B genotypes. Moreover, three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected.
    Źródło:
    Wiadomości Parazytologiczne; 2010, 56, 2; 171-177
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Współczesne poglądy na odrębność gatunkową Giardia i specyficzność żywicieli
    Contemporary view on Gardia species and specificity of their hosts
    Autorzy:
    Kasprzak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/836474.pdf
    Data publikacji:
    1988
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    pasozyty
    Giardia
    odrebnosc gatunkowa
    taksonomia
    zmiennosc taksonomiczna
    zywiciele
    specyficznosc
    Opis:
    The data presented in the paper indicate a probable lack of host specificity in Giardia and a number of species lower than that described to date. The common opinion is that three morphological groups should be recognized, represented by G. intestinalis (= G. duodenalis), G. muris and G. agilis. The diagnostics within these groups should be based on analysis of their isoenzymes or DNA fragments, and on antigene differences.
    Źródło:
    Annals of Parasitology; 1988, 34, 4-6
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Współczesne poglądy na odrębność gatunkową Giardia i specyficzność żywicieli
    Contemporary view on Gardia species and specificity of their hosts
    Autorzy:
    Kasprzak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2152677.pdf
    Data publikacji:
    1988
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia
    pasozyty
    Giardia
    odrebnosc gatunkowa
    taksonomia
    zmiennosc taksonomiczna
    zywiciele
    specyficznosc
    Opis:
    The data presented in the paper indicate a probable lack of host specificity in Giardia and a number of species lower than that described to date. The common opinion is that three morphological groups should be recognized, represented by G. intestinalis (= G. duodenalis), G. muris and G. agilis. The diagnostics within these groups should be based on analysis of their isoenzymes or DNA fragments, and on antigene differences.
    Źródło:
    Wiadomości Parazytologiczne; 1988, 34, 4-6; 459-467
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wartosc diagnostyczna testow wykrywajacych swoiste przeciwciala Giardia
    Autorzy:
    Kurczewska, M
    Paul, M.
    Pawlowski, Z.
    Skoryna, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/838538.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    choroby inwazyjne
    lamblia
    pasozyty zwierzat
    Giardia
    przeciwciala
    parazytologia
    diagnostyka
    giardioza
    pasozyty czlowieka
    Opis:
    Two commercial faecal antigen tests, based on Giardia specific antigens, were compared with traditional microscopical examination of faeces in 40 clinical patients, including 14 patients with a confirmed Giardia infection as well as in a survey among 96 children in four day care centers. The results obtained with these faecal antigen tests were not much better than by using traditional microscopy by a well trained parasitologist. Specific Giardia antigen (GSA 65) has been still detected in faeces up to day 4th after treatment of giardiosis, when microscopical examination of faeces was already for a few days negative. The Giardia antigen detection test was not very useful in examining the unconcentrated duodenal content.
    Źródło:
    Annals of Parasitology; 1994, 40, 1; 11-14
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wartość diagnostyczna testów wykrywających swoiste przeciwciała Giardia
    DIAGNOSTIC VALUE OF THE SPECIFIC GIARDIA FAECAL ANTIGEN TESTS*
    Autorzy:
    Kurczewska, M.
    Paul, M.
    Pawłowski, Z.
    Skoryna, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2151424.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    choroby inwazyjne
    lamblia
    pasozyty zwierzat
    Giardia
    przeciwciala
    parazytologia
    diagnostyka
    giardioza
    pasozyty czlowieka
    Opis:
    Two commercial faecal antigen tests, based on Giardia specific antigens, were compared with traditional microscopical examination of faeces in 40 clinical patients, including 14 patients with a confirmed Giardia infection as well as in a survey among 96 children in four day care centers. The results obtained with these faecal antigen tests were not much better than by using traditional microscopy by a well trained parasitologist. Specific Giardia antigen (GSA 65) has been still detected in faeces up to day 4th after treatment of giardiosis, when microscopical examination of faeces was already for a few days negative. The Giardia antigen detection test was not very useful in examining the unconcentrated duodenal content.
    Źródło:
    Wiadomości Parazytologiczne; 1994, 40, 1; 11-14
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potential sources of infection with selected zoonotic agents in the veterinary work environment - pilot studies
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Sawczyn-Domańska, A.
    Kloc, A.
    Zwoliński, J.
    Kłapeć, T.
    Studzińska, M.B.
    Chmura, R.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085448.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    environment
    Coxiella burnetii
    Toxoplasma gondii
    veterinarians
    Leptospira spp
    Cryptosporidium spp
    Giardia duodenalis
    Opis:
    Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 146-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serotonin level in blood and monoaminooxidase [MAO] activity in blood serum in children infected with Giardia intestinalis
    Autorzy:
    Lucinska, A.
    Galary, E.
    Prusek, W.
    Podwysocka, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/837258.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    infection
    clinical symptom
    Giardia
    monoaminooxidase activity
    serotonin level
    giardiasis
    blood
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wystepowanie gatunkow i genotypow Giardia u ludzi i zwierzat w Wielkopolsce
    Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
    Autorzy:
    Solarczyk, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/837534.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Wielkopolska
    wystepowanie
    pasozyty jelitowe
    pasozyty zwierzat
    genotyp
    Giardia
    pierwotniaki
    gatunki
    pasozyty czlowieka
    Źródło:
    Annals of Parasitology; 2009, 55, 4; 459-462
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro susceptibility of Giardia isolates of human and animal origin to natamycin
    Autorzy:
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/837538.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    metronidazole
    man
    Giardia
    drug
    giardiasis
    animal
    natamycin
    Protozoa
    ornidazole
    in vitro
    parasitosis
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie gatunków i genotypów Giardia u ludzi i zwierząt w Wielkopolsce
    Occurrence of Giardia species and genotypes in humans and animals in Wielkopolska region, Poland
    Autorzy:
    Solarczyk, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143568.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Wielkopolska
    wystepowanie
    pasozyty jelitowe
    pasozyty zwierzat
    genotyp
    Giardia
    pierwotniaki
    gatunki
    pasozyty czlowieka
    Opis:
    Giardia is the most common intestinal protozoan parasite found in humans and animals worldwide. Although it has been known for three hundred years, the nomenclature, taxonomy, host specificity, and pathogenicity of Giardia still arouse numerous controversies and ambiguities. Giardia is classified into six species, that are characterised by various ranges of hosts. The most dubious species is G. intestinalis, which includes a dozen or so genotypes, and only two of them (genotype A and B) have wide ranges of hosts, including humans. Moreover, in some genotype assemblages of G. intestinalis certain subgenotypes were distinguished and it was proven that in the same host species various subgenotypes of this parasite may occur. Bearing in mind the significant genetic heterogeneity of G. intestinalis and the fact that various genotypes and subgenotypes of this parasite are characterised by the broad or narrow host specificity, the data concerning the frequency of giardiosis occurrence are insufficient. It is necessary to use molecular biology techniques in order to define the genotype and/or the subgenotype of G. intestinalis that are found in humans and in certain animal species. Furthermore, since more and more pieces of evidence connected with a possibility of the sexual recombination of Giardia are gathered, it is unknown if genotypes and subgenotypes of this parasite are stable in time. The aim of this thesis was to define the frequency of Giardia occurrence in humans and animals in Wielkopolska region, to identify species and genotypes of Giardia that occur in humans and animals, as well as to obtain an axenic culture of the chosen isolates of Giardia from animals and to compare the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites in order to check if the axenisation of G. intestinalis leads to the selection of genotypes or if Giardia genotypes are stable in time. Altogether, 2183 faecal samples were examined for the presence of Giardia cysts; 447 faecal samples were taken from 232 persons coming from 5 cities situated in Wielkopolska, and 1736 faecal samples were obtained from 123 animal species, including: 266 faecal samples from 113 species of animals kept in the Zoological Garden in Poznań, 1286 samples from 4 species of breeding animals, 118 samples from dogs, and 66 samples from 5 species of wild animals. Faecal samples were taken from animals coming from 25 places in Wielkopolska. Moreover, seven isolates of G. intestinalis were used in the studies, which were obtained from various species of hosts and kept in an axenic in vitro culture. Microscopic, molecular and bio-informative methods were used in the studies. From each faecal sample fresh smears were made in a 0.6% solution of physiological salt and in Lugol’s solution, as well as a permanent smear stained with trichrome was made. Moreover, the following molecular techniques were implemented in the studies: DNA extraction and purification, the PCR technique (two molecular markers), electrophoresis and visualisation of PCR products, and sequencing. A fragment of the b-giardin gene was used as a molecular marker in order to define the genotype and subgenotype of Giardia. Only in the case of genotyping of two Giardia isolates obtained from Peromyscus eremicus another molecular marker (SSU rRNA)was additionally used. Some widely available computer programmes (Chromas, CAP 3, BioEdit, BLASTn, MEGA version 4.0) were utilised in the analysis of the sequence of the b-giardin gene fragment and in the phylogenetic analysis. The culture of Giardia trophozoites was established to compare the sequence of the partial b-giardin gene from cysts and trophozoites. Concentration and purification of Giardia cysts in the saccharose gradient, and the excystation technique were applied in the studies to obtaining an axenic in vitro culture. In this study, Giardia cysts were found in 12 faecal samples obtained from 3 persons and 5 animal species. Giardia cysts were found only in faecal samples from humans living in Poznań and the samples obtained from animals coming from Poznań and around Puszczykowo. The highest frequency of infection was stated in domestic animals (2.5%) and in animals kept in the Zoological Garden (2.0%), whereas a slightly lower frequency was noticed in wild animals (1.5%) and in humans (1.3%). No Giardia cysts were found in the faecal samples collected from breeding animals. Two new species of Giardia hosts were identified, namely Rhinella marina and Peromyscus eremicus; however, due to a minimal amount of faecal samples supplied for the study it was impossible to define the species and genotype of this parasite. PCR products (the partial of b-giardin gene) were obtained in seven faecal samples out of the ten studied, including three samples from people and four faecal samples derived from three animal species (i.e. dog, tamandua, red deer). Moreover, molecular characterization of seven Giardia isolates from three persons and four animal species (red-bellied monkey, silver marmoset, Thomson’s gazelle, and sheep) kept in an axenic in vitro culture was performed. Based on the b-giardin sequence fragment analysis, four assemblages of G. intestinalis genotypes were identified (A, B, C and D). In humans, A and B G. intestinalis genotypes and three subgenotypes, including a cosmopolitan subgenotype A2 and two new subgenotypes A and B were detected. Furthermore, four G. intestinalis genotypes were found in animals, including three genotypes which are non-infectious to humans, namely: genotypes C and D in dogs and a cervids-specific genotype A in red deer (Cervus elaphus), which indicate that these animals do not constitute the source of infection to humans. On the other hand, in a tamandua from the Zoological Garden in Poznań a new subgenotype B of G. intestinalis was identified, which due to a close relationship with Giardia isolates obtained from humans is potentially infectious to man. In none of the studied faecal samples a mixed infection of Giardia was found. To date, nine sequences of the partial b-giardin gene have been deposited in the National Center for Biotechnology Information (NCBI), including two sequences of Giardia isolates obtained from humans (GenBank accession numbers FJ009207, FJ009208), three sequences of isolate obtained from red deer (GenBank accession numbers EU621373, EU626198, EU216429), two sequences of both Giardia isolates obtained from dogs (GenBank accession numbers FJ009205, FJ009206), and the single sequences obtained from tamandua (GenBank accession number FJ009209) and from Thomson’s gazelle (GenBank accession number EU626199). According to the literature, an axenic in vitro culture of G. intestinalis was obtained from a red deer for the first time. Based on the analysis of the sequence of the b-giardin gene fragment obtained from the DNA isolated from cysts and trophozoites it was proven that the red deer was infected with a single population of Giardia and that during the axenisation of the culture no mutation in the DNA of the parasite’s trophozoites took place. Probably the time distance that the DNA was isolated from the trophozoites kept in the culture was too short to cause the mutation. This suggestion is confirmed by the results of the genotyping of seven G. intestinalis isolates obtained from various host species and kept in an axenic in vitro culture for at least a number of years. Based on the molecular characteristics it was stated that all the studied isolates from the axenic culture were identical and belonged to the same assemblage B. The comparision with the sequences from GenBank database revealed that all mentioned isolates were 99% similar to the sequence of Giardia Nij5 isolate obtained from a person from the Netherlands and characterised as genotype B1. Due to the sameness of the molecular marker sequences it seems improbable that the identical G. intestinalis genotype occurred in various time periods (the largest difference was 14 years) in humans and in a number of animal species in diverse areas of Wielkopolska region. Quite opposite, the long-term keeping of these isolates in the homogenous conditions of an axenic in vitro culture leads to the selection of a genotype or proves the instability of genotypes of this parasite. Long-term studies need to be conducted in order to verify these hypothesis. Their results will have a key meaning in explaining the genetic structure of the Giardia population and in understanding the molecular epidemiology of giardiosis.
    Źródło:
    Wiadomości Parazytologiczne; 2009, 55, 4; 459-462
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiologiczne badania nad giardioza u mieszkancow Szczecina
    Autorzy:
    Ramisz, A
    Krolikowska, A.
    Balicka-Ramisz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/840500.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Szczecin
    choroby pasozytnicze
    choroby czlowieka
    Giardia intestinalis
    pierwotniaki
    parazytologia
    giardioza
    epidemiologia
    mieszkancy
    pasozyty czlowieka
    Opis:
    The aim of the studies was to establish the prevalence of Giardia intestinalis in Szczecin population. The studies were carried out in 1991-1996. In this period 46 584 persons - 22 371 adults and 24 213 children were examined. Faecal samples were examined with two methods - coprological and immunoenzymatic (ELISA) test. A total of 356 persons (0.78%) were found to be infected with G. intestinalis. No statistically significant differences between the infection rate of adults and children were observed. The adults were infected in 0.73% and the children in 0.80%. The analysis of the annual dynamics of G. intestinalis infection shows twice as large infection rate in September- October (1.18 %) as in the remaining months (0.66 %).
    Źródło:
    Annals of Parasitology; 1999, 45, 1; 69-74
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiologiczne badania nad giardiozą u mieszkańcow Szczecina
    EPIDEMIOLOGICAL STUDIES ON GIARDIOSIS IN INHABITANTS OF SZCZECIN
    Autorzy:
    Ramisz, A.
    Królikowska, A.
    Balicka-Ramisz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148719.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Szczecin
    choroby pasozytnicze
    choroby czlowieka
    Giardia intestinalis
    pierwotniaki
    parazytologia
    giardioza
    epidemiologia
    mieszkancy
    pasozyty czlowieka
    Opis:
    The aim of the studies was to establish the prevalence of Giardia intestinalis in Szczecin population. The studies were carried out in 1991-1996. In this period 46 584 persons - 22 371 adults and 24 213 children were examined. Faecal samples were examined with two methods - coprological and immunoenzymatic (ELISA) test. A total of 356 persons (0.78%) were found to be infected with G. intestinalis. No statistically significant differences between the infection rate of adults and children were observed. The adults were infected in 0.73% and the children in 0.80%. The analysis of the annual dynamics of G. intestinalis infection shows twice as large infection rate in September- October (1.18 %) as in the remaining months (0.66 %).
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 1; 69-74
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Selected cytokines and morphological parameters of blood in patients with Giardia intestinalis or Toxoplasma gondii
    Autorzy:
    Czyzewska, J.
    Matowicka-Karna, J.
    Kemona, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/5611.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    cytokine
    morphological parameter
    blood
    patient
    Giardia intestinalis
    Toxoplasma gondii
    human disease
    giardiasis
    toxoplasmosis
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Source and significance of genetic polymorphism of selected parasitic Protozoa
    Autorzy:
    Majewska, A.C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/838111.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    polymorphism
    control
    Giardia
    Trypanosoma
    epidemiology
    evolution
    parasite population
    Cryptosporidium
    Protozoa
    Toxoplasma
    taxonomy
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spostrzezenia nad skutecznoscia ornidazolu [Tiberal La Roche] w leczeniu giardiozy
    Autorzy:
    Kuzmicki, R
    Jeske, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/841080.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    lamblia
    Giardia
    czynniki chorobotworcze
    choroby czlowieka
    farmakologia
    ornidazol
    skutecznosc
    parazytologia
    leczenie
    giardioza
    leki
    Opis:
    Ornidazole (Tiberal La Roche) was used in 50 persons infected with Giardia intestinalis in the dosis of 500 mg twice a day during 5 days. Examinations were performated after 7-10 days, having been repeated one month and 3 months later. A positive results were achieved after one month in 46 cases (92%) and after 3 months in 45 cases (90%). Tiberal is thought to be effective drug in the treatment of Giardiosis, causing no side effect apart from headache and nausea occurring sporadically.
    Źródło:
    Annals of Parasitology; 1994, 40, 1; 65-68
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spostrzeżenia nad skutecznością ornidazolu [Tiberal La Roche] w leczeniu giardiozy
    OBSERVATIONS OF THE EFFICACY OF ORNIDAZOLE (TIBERAL LA ROCHE) IN TREATMENT OF GIARDIOSIS
    Autorzy:
    Kuźmicki, R.
    Jeske, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2151432.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    lamblia
    Giardia
    czynniki chorobotworcze
    choroby czlowieka
    farmakologia
    ornidazol
    skutecznosc
    parazytologia
    leczenie
    giardioza
    leki
    Opis:
    Ornidazole (Tiberal La Roche) was used in 50 persons infected with Giardia intestinalis in the dosis of 500 mg twice a day during 5 days. Examinations were performated after 7-10 days, having been repeated one month and 3 months later. A positive results were achieved after one month in 46 cases (92%) and after 3 months in 45 cases (90%). Tiberal is thought to be effective drug in the treatment of Giardiosis, causing no side effect apart from headache and nausea occurring sporadically.
    Źródło:
    Wiadomości Parazytologiczne; 1994, 40, 1; 65-68
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dwadziescia lat badan nad Cryptosporidium spp. i Giardia spp. w Polsce
    Cryptosporidium spp. and Giardia spp. - environmental studies in Poland
    Autorzy:
    Bajer, A.
    Bednarska, M.
    Sinski, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/841611.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pasozyty zwierzat
    pasozyty jelitowe
    Giardia
    Polska
    parazytologia
    rezerwuary pasozytow
    badania epidemiologiczne
    pasozyty czlowieka
    Cryptosporidium
    Źródło:
    Annals of Parasitology; 2009, 55, 4; 301-304
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wspolwystepownie Cryptosporidium parvum, Giardia spp. i helmintow w populacjach drobnych gryzoni
    Autorzy:
    Bajer, A.
    Behnke, J.M.
    Bednarska, M.
    Kulis, K.
    Sinski, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/837176.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Giardia
    parazytologia
    Mazurski Park Krajobrazowy
    wspolwystepowanie
    populacje zwierzat
    nicienie
    pasozyty
    Cryptosporidium parvum
    gryzonie
    tasiemce
    Źródło:
    Annals of Parasitology; 2004, 50, 2; 307-315
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Współwystępownie Cryptosporidium parvum, Giardia spp. i helmintów w populacjach drobnych gryzoni
    The co-occurrence of Cryptosporidium parvum, Giardia spp. and helminth infections in small rodent populations
    Autorzy:
    Bajer, A.
    Behnke, J.M.
    Bednarska, M.
    Kuliś, K.
    Siński, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146546.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Giardia
    parazytologia
    Mazurski Park Krajobrazowy
    wspolwystepowanie
    populacje zwierzat
    nicienie
    pasozyty
    Cryptosporidium parvum
    gryzonie
    tasiemce
    Opis:
    During long-term (1998-2000) studies on rodent parasite populations in Mazury lake district there were collected and analyzed data on co-occurrence of intestinal protozoa (Cryptosporidium parvum, Giardia spp.) and helminths. There were performed 178 autopsies of common vole Microtus arvalis, 85 autopsies of yellow-necked mouse Apodemus flavicollis and 386 autopsies of bank vole Clethrionomys glareolus. Positive effect of helminth infections was found in C. glareolus. Voles infected with nematode Heligmosomum mixtum showed higher prevalence of C. parvum and Giardia spp. than voles infected with Heligmosomoides glareoli. The host age took part in these interactions and positive effect of co-occurrence was mainly observed in voles older than 3 months. The other intrinsic (host sex) or extrinsic (season and year of study) factors influenced interactions between parasites. Presented results revealed that helminth infections may facilitate chronic infections of intestinal protozoa in rodent populations.
    Źródło:
    Wiadomości Parazytologiczne; 2004, 50, 2; 307-315
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dwadzieścia lat badań nad Cryptosporidium spp. i Giardia spp. w Polsce
    Cryptosporidium spp. and Giardia spp. - environmental studies in Poland
    Autorzy:
    Bajer, A.
    Bednarska, M.
    Siński, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143545.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pasozyty zwierzat
    pasozyty jelitowe
    Giardia
    Polska
    parazytologia
    rezerwuary pasozytow
    badania epidemiologiczne
    pasozyty czlowieka
    Cryptosporidium
    Opis:
    Cryptosporidium spp. and Giardia spp. are intestinal protozoan parasites of humans and many other species of mammals. The aim of this article was to summarize the last twenty years of research on the environmental distribution of these parasites, with a particular emphasis on the natural reservoir of invasion and human infections in Poland. The prevalence of Cryptosporidium and Giardia has been studied in different groups of humans, in wildlife, pets and farm animals and in environmental samples. Current knowledge on the distribution of zoonotic and non-zoonotic species/genotypes in reservoir hosts and environmental samples has been summarized. The usefulness of different methods for the detection and identification of the parasites in different types of samples has been presented. Due to the wide distribution and high prevalence of both species in a range of hosts and possible vectors involved in mechanical transmission, the overall risk of outbreaks of cryptosporidiosis and giardiosis in Poland has been assessed as relatively high.
    Źródło:
    Wiadomości Parazytologiczne; 2009, 55, 4; 301-304
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Uklad Giardia - zywiciel: zmiennosc obrazu inwazji
    Autorzy:
    Majewska, A C
    Powiązania:
    https://bibliotekanauki.pl/articles/836470.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    interakcje zywiciel-pasozyt
    choroby inwazyjne
    lamblia
    pasozyty zwierzat
    Giardia
    parazytologia
    giardioza
    inwazja pasozytnicza
    zywiciele
    pasozyty czlowieka
    Opis:
    In man, as well as in many species of vertebrates there exist several populations of Giardia duodenalis group which, though morphologically indistinguishable, show different level of heterogenicity in several characteristics, a. o. in surface antigens, isoenzyme make-up, RFLP, invasiveness for different hosts or pattern of experimental infection. Also the clinical observations in man distinctly suggested that G. intestinalis comprises several different populations. In the course of giardiosis apparent variability in clinical manifestations can be observed. In many patients the infection is symptomless and resolves spontaneously and in some others – variable intensity of symptoms is observed. Most likely both the parasit's characteristics and the host's feature will determine the clinical character of infection. It is well known that in some cases it is the host that responsible for the symptoms of the infection. Thus, for instance, clinical giardiosis links with immunodeficiency, malnutrition or young age. There are also evidences that some Giardia isolates have enhanced potency to provoke the disease of the host. It is considered that the variable pattern of the infection may be related to three different factors in host-parasite relationship: extra- and intrapopulation variability of Giardia isolates, the microenvironmental factors of the host's intestine, and the variable immune response of the host. The author describes data on the two first factors.
    Źródło:
    Annals of Parasitology; 1994, 40, 1; 3-9
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The raccoon (Procyon lotor) as a reservoir of zoonotic diseases
    Autorzy:
    Solarczyk, P.
    Osten-Sacken, N.
    Heddergott, M.
    Frantz, A.
    Jankowska, W.
    Majewska, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/5791.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    racoon
    Procyon lotor
    Baylisascaris procyonis
    Giardia
    reservoir
    zoonotic disease
    prevalence
    helminth
    intestinal parasite
    Protozoa
    human disease
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Układ Giardia - żywiciel: zmienność obrazu inwazji
    Autorzy:
    Majewska, A. C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2151421.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    interakcje zywiciel-pasozyt
    choroby inwazyjne
    lamblia
    pasozyty zwierzat
    Giardia
    parazytologia
    giardioza
    inwazja pasozytnicza
    zywiciele
    pasozyty czlowieka
    Opis:
    In man, as well as in many species of vertebrates there exist several populations of Giardia duodenalis group which, though morphologically indistinguishable, show different level of heterogenicity in several characteristics, a. o. in surface antigens, isoenzyme make-up, RFLP, invasiveness for different hosts or pattern of experimental infection. Also the clinical observations in man distinctly suggested that G. intestinalis comprises several different populations. In the course of giardiosis apparent variability in clinical manifestations can be observed. In many patients the infection is symptomless and resolves spontaneously and in some others – variable intensity of symptoms is observed. Most likely both the parasit's characteristics and the host's feature will determine the clinical character of infection. It is well known that in some cases it is the host that responsible for the symptoms of the infection. Thus, for instance, clinical giardiosis links with immunodeficiency, malnutrition or young age. There are also evidences that some Giardia isolates have enhanced potency to provoke the disease of the host. It is considered that the variable pattern of the infection may be related to three different factors in host-parasite relationship: extra- and intrapopulation variability of Giardia isolates, the microenvironmental factors of the host's intestine, and the variable immune response of the host. The author describes data on the two first factors.
    Źródło:
    Wiadomości Parazytologiczne; 1994, 40, 1; 3-9
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genotyping of Giardia duodenalis human isolates using PCR-RFLP in Zabol City, East of Iran
    Autorzy:
    Abedi, M.
    Dabirzadeh, M.
    Ghasemian, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/6472.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    genotyping
    Giardia duodenalis
    protozoan parasite
    parasite
    human disease
    isolation
    PCR-RFLP method
    Zabol city
    Iran
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Cryptosporidium, Giardia and Toxoplasma in surface waters in the area of Cracow
    Autorzy:
    Polus, M.
    Kocwa-Haluch, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/207841.pdf
    Data publikacji:
    2014
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    protozoa
    surface waters
    Cryptosporidiosis
    Cryptosporidium
    Giardia intestinalis
    oocysts
    water samples
    pierwotniaki
    wody powierzchniowe
    kryptosporydioza
    oocysty
    próbki wody
    Opis:
    Despite some knowledge on the risks from exposure to intestinal parasitic protozoa such as Cryptosporidium, Giardia and Toxoplasma, no occurrence studies of these protozoa were conducted in Cracow area. Therefore, the purpose of the work was to examine the presence of Cryptosporidium, Giardia and Toxoplasma in surface water samples including intakes and recreational waters. The study showed that the vast majority of water samples were positive for Cryptosporidium sp., and some of the samples were positive for Giardia intestinalis. None of the tested water samples showed the presence of Toxoplasma. Given the limitations of the research methods used and the limits of detection, these results indicated that the concentration of protozoan cysts/oocysts may range from <1 to dozens oocysts/cysts per liter of water. These concentrations are comparable to the values given in reports of major worldwide outbreaks of cryptosporidiosis or giardiasis, which indicating a need to further investigate Cracow's surface waters.
    Źródło:
    Environment Protection Engineering; 2014, 40, 2; 105-113
    0324-8828
    Pojawia się w:
    Environment Protection Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Redefining parasite life cycles through epidemiology, pathology and developmental biology
    Autorzy:
    Conn, D.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/5835.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasite
    life cycle
    epidemiology
    pathology
    developmental biology
    Cryptosporidium
    Giardia
    Encephalitozoon
    Enterocytozoon
    Brandesia
    Prosotocus
    Maritrema
    Mediogonimus
    Echinostoma
    Ornithodiplostomum
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metabolizm serotoniny u dzieci zarażonych Giardia intestinalis
    Autorzy:
    Lucińska, A.
    Prusek, W.
    Galary, E.
    Podwysocka, M.
    Modrzycka, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148530.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    choroby pasozytnicze
    parazytologia lekarska
    serotonina
    Giardia intestinalis
    dzieci
    pierwotniaki
    reakcje immunologiczne
    metabolizm
    giardioza
    inwazja pasozytnicza
    pasozyty czlowieka
    Opis:
    According to the data from the last 4 years, 3.8% of all diagnosed parasitoses are giardiases. During 5 years Giardia intestinalis was found in 24 chiidren hospitalized in the General Hospital of the Wrocław Voivodship: in 14 girls and 10 boys, 2-12 years old. The parasite was identified coproscopically and on the basis of clinical symptoms. Before the medication, the blood was taken from each child to determine the serotonin level and the (MAO) monoaminooxydaze, enzyme metabolizing serotonin acivity in the blood serum. The results were compared to the values obtained in the control group of 10 healthy children, 3-12 years old. III children were divided into two groups: first consisted of 12 children, 2-5 years old, second - of 12 children, 6-12 years old. In the first group 210% increase of the serotonin level in the blood and slight 32,4% increase of the MAO activity were observed in comparison with the control group. In the second group the serotonin level in the blood increased 221 % in comparison with the control, while the MAO actmty increased 45.1 %. In older children marced clinical symptoms could be observed, mainly malfunction of the alimentary tract, microelements shortage and anemia. The highest level of serotonin and MAO activity was found in the child with bile duet irritation. The reason for the increased level of serotonin and MAO activity can be pathogenic action of parasite's trophozoites and cysts on the jejunum mucosa and mobilization, proliferation and degranulation of tissue mastocytes. Serotonin, released from mastocytes, can be the cause of enhanced peristalsis and mucosa congestion.
    Źródło:
    Wiadomości Parazytologiczne; 2000, 46, 1; 149-155
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zwierzęta ziemnowodne jako źródło zanieczyszczenia wody Cryptosporidium i Giardia
    Semi-aquatic animals as a source of water contamination with Cryptosporidium and Giardia
    Autorzy:
    Bajer, A.
    Bednarska, M.
    Paziewska, A.
    Romanowski, J.
    Siński, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143808.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pizmak
    bobr europejski
    zanieczyszczenia biologiczne
    Giardia
    pierwotniaki
    rezerwuary pasozytow
    pasozyty
    zwierzeta ziemnowodne
    zrodla zanieczyszczen
    Cryptosporidium
    zanieczyszczenia wod
    Opis:
    Cryptosporidium and Giardia spp. are parasitic protozoa localized in the alimentary tract of many animal species and humans. Each of these parasite species produces very resistant invasive forms (cysts and oocysts) excreted to the environment with feces of infected hosts. Water contaminated with cysts/oocysts constitutes one of the main transmission routes and is responsible for the majority of infections in humans. Cryptosporidium and Giardia spp. were found in many different species of animals, including livestock, pets and free living animals. The aim of our study was to determine the prevalence of these protozoa in selected species of semi−aquatic mammals and to estimate their role in water contamination. In years 1996–98 the prevalence of Cryptosporidium and Giardia infections was high in muskrats (Ondatra zibethicus) (58 and 87%, respectively). The origin of animals (farmed or free living) affected the prevalence of both parasites in European beavers (Castor fiber). The prevalence of infection increased in second period of study and was 4 and 19% for Cryptosporidium and 0 and 8% for Giardia spp. in the two studied periods, respectively. Both parasite species were also identified in water vole (Arvicola terrestris) and rat (Rattus norvegicus).
    Źródło:
    Wiadomości Parazytologiczne; 2008, 54, 4; 315-318
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of Giardia intestinalis assemblages in formalin fixed stool samples collected from school children in the Ghazni Province, eastern Afghanistan
    Autorzy:
    Lass, A.
    Korzeniewski, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/6510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    detection
    Giardia intestinalis
    child
    school child
    intestinal parasite
    parasitic disease
    human disease
    prevalence
    Ghazni province
    Afghanistan
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zrodlo i znaczenie genetycznego zroznicowania populacji pasozytniczych pierwotniakow
    Autorzy:
    Majewska, A C
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/837049.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Annals of Parasitology; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Źródło i znaczenie genetycznego zróżnicowania populacji pasożytniczych pierwotniaków
    SOURCE AND SIGNIFICANCE OF GENETIC POLYMORPHISM OF SELECTED PARASITIC PROTOZOA
    Autorzy:
    Majewska, A. C.
    Sulima, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2148795.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    zroznicowanie genetyczne
    Leishmania
    Giardia
    struktura populacji
    pierwotniaki
    parazytologia
    zroznicowanie gatunkowe
    Toxoplasma
    populacje zwierzat
    pasozyty
    Trypanosoma
    Cryptosporidium
    Opis:
    The application of biochemical and molecular techniques in parasitological studies has provided increasing evidences of genetic polymorphism among parasite populations. This review presents possible origins of genetic variation within populations of various protozoan species. Since the mode of reproduction has an important influence on genetic polymorphism within parasite populations these considerations refer mainly to some protozoan parasites which have various life cycles, e.g. Giardia, Trypanosoma, Cryptosporidium, Toxoplasma. Also other factors associated with parasites (such as: transmission and passage history in laboratory conditions; occurrence in different hosts or geographic regions; selective pressure of drugs; competitive interactions between populations) that affect parasite genetic diversity are discussed. However, the number of examined isolates of parasites and genetic markers, assortment of methods, probes, primers and reagents used is also of significance. The significance of genetic variability in parasite populations is still the subject of much interest and controversy. A simple interpretation of such variation is impossible because of the complexity of host-parasite interactions. The knowledge of parasite diversity at the nucleic acids level has continually increased, but a corect interpretation of this phenomenon requires at least the same knowledge of genetic variability in host populations. Nevertheless, genetic variability in protozoan parasites has many important implications, e.g. for taxonomy, epidemiology, control and evolution. Genetic differences within parasite populations might also be associated with phenotypic variability, e.g. virulence, antigenicity, infectivity, drug sensitivity, hostpreference etc.
    Źródło:
    Wiadomości Parazytologiczne; 1999, 45, 3; 293-307
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie Giardia intestinalis u psów domowych w Warszawie
    Prevalence of Giardia intestinalis in domestic dogs in Warsaw
    Autorzy:
    Zygner, W.
    Jaros, D.
    Skowrońska, M.
    Bogdanowicz-Kamirska, M.
    Wędrychowicz, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144594.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wystepowanie
    pasozyty zwierzat
    psy
    diagnostyka parazytologiczna
    choroby pasozytnicze
    Giardia intestinalis
    parazytologia
    konferencje
    giardioza
    Warszawa
    choroby zwierzat
    Warszawa konferencja
    Opis:
    Introduction. Giardia intestinalis is the most common intestinal protozoan parasite, which infects humans, dogs and other mammals throughout the world. So far eight genotypes of the parasite have been described of which four were found in dogs. Assemblages A-I and B infect either dogs or humans. Assemblages C and D occur only in dogs. The aim of the study was to determine the prevalence and genotypes of G. intestinalis in domestic dogs of Warsaw area. Material and methods. From October 2005 to March 2006 fecal samples were collected from 350 dogs and examined using light microscopy and PCR techniques. Results. 5.14% of dogs was found to be positive for G. intestinalis by microscopy and prevalence of 9.14% was found by PCR. The PCR amplicons were sequenced and the DNA sequences were compared with Giardia sequences in GeneBank database. The analysis revealed assemblage A-I in 1.71% of dogs, assemblage C in 1.14% and assemblage D in 6.28% of dogs in Warsaw. According to literature, the genotype A can infect humans however a role of dogs as a reservoir of human giardiosis in Poland is not known.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 4; 311-315
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optymalizacja metody wykrywania pierwotniaków pasożytniczych Cryptosporidium i Giardia w środowisku wodnym przy zastosowaniu automatycznej stacji płuczącej Filta-Max xpress
    Optimization of Cryptosporidum and Giardia detection in water environment using automatic elution station Filta-Max xpress
    Autorzy:
    Matuszewska, R
    Szczotko, M
    Krogulska, B
    Powiązania:
    https://bibliotekanauki.pl/articles/872081.pdf
    Data publikacji:
    2012
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    srodowisko wodne
    analiza wody
    pierwotniaki
    pasozyty
    wykrywanie
    Cryptosporidium
    Giardia
    uklady pluczace
    automatyczna stacja pluczaca Filta-Max xpress
    Opis:
    Wprowadzenie. Obecność pierwotniaków pasożytniczych w wodzie przeznaczonej do spożycia jest przeważnie następstwem niewłaściwie prowadzonych procesów jej uzdatniania. Aktualnie, w Polsce, rutynowe badania wody w kierunku wykrywania pierwotniaków z rodzaju Cryptosporidium i Giardia nie są prowadzone. Cel badań. Celem badań była optymalizacja metody wykrywania oocyst Cryptosporidium sp. i cyst Giardia sp. w próbkach wody powierzchniowej w oparciu o założenia metodyczne zawarte w normie ISO 15553:2006 i przy zastosowaniu automatycznej stacji płuczącej Filta-Max xpress. Materiał i metoda. Badania wstępne prowadzono na próbkach wody kontaminowanej oocytami i cystami szczepów referencyjnych Cryptosporidium sp. i Giardia sp. Dalsze badania prowadzono z zastosowaniem próbek środowiskowych wody pobranej z kilku ujęć wody powierzchniowej (21 próbek z Wisły i 8 próbek z Zalewu Zegrzyńskiego). Próbki wody zagęszczano przy użyciu stacji Filta-Max xpress, a następnie oczyszczano je metodą separacji immunomagnetycznej. Cysty i oocysty barwiono za pomocą FITC i DAPI i prowadzono obserwacje mikroskopowe z wykorzystaniem mikroskopu epifluorescencyjnego. Wyniki. Zastosowanie 9-cyklowego systemu płuczącego w badaniach fortyfikowanych próbek wody, pozwoliło na uzyskanie średniego odzysku oocyst Cryptosporidium w wysokości 60,6% i cyst Giardia w wysokości 36,1%. W próbkach środowiskowych wody pochodzącej z ujęć powierzchniowych wykazano obecność poszukiwanych pierwotniaków. Cysty Giardia wykryto we wszystkich badanych próbkach wody powierzchniowej w liczbie od 1,0/10 l do 4,5/10 l pochodzącej z ujęcia na Zalewie Zegrzyńskim i z ujęcia na rzece Wiśle w liczbie od 1,0/10 l do 38,9/10 l. Oocysty Cryptosporidium obecne były w 50% próbek wody powierzchniowej z ujęć Zalewu Zegrzyńskiego i 47,6% próbek z Wisły, a ich liczba w obu przypadkach była zbliżona i wynosiła od 0,5 do 2,5 oocyst/10 l. Badania potwierdziły, że zastosowana metoda jest właściwa do wykrywania obecności pierwotniaków pasożytniczych w wodzie. Jej efektywność, szczególnie w przypadku próbek o dużej zawartości substancji organicznych i zawiesin, zależy od przygotowanych podpróbek i zmniejszenia szybkości płukania filtrów. Wnioski. Metoda wykrywania Cryptosporidium sp. i Giardia sp. z zastosowaniem automatycznej stacji płuczącej Filta- -Max xpress może być wykorzystywana w rutynowych badaniach oceny mikrobiologicznej jakości wody. Stwierdzanie obecności Cryptosporidium sp. i Giardia sp. w próbkach wody pochodzącej z ujęć wody powierzchniowej wskazuje na niebezpieczeństwo przedostawania się ich do wody przeznaczonej do spożycia, dlatego badania pierwotniaków pasożytniczych powinny być włączone do monitoringu wody.
    Background. The presence of parasitic protozoa in drinking water is mostly a result of improperly maintened the water treatment process. Currently, in Poland the testing of Cryptosporidium and Giardia in water as a part of routine monitoring of water is not perform. Objective. The aim of this study was the optimization of the method of Cryptosporidium and Giardia detection in water according to the main principles of standard ISO 15553:2006 and using Filta-Max xpress automatic elution station. Material and method. Preliminary tests were performed on the samples contaminated with oocysts and cysts of reference strains of both parasitic protozoa. Further studies were carried out on environmental samples of surface water sampled directly from the intakes of water (21 samples from Vistula River and 8 samples from Zegrzynski Lake). Filtration process and samples volume reducing were performed using an automatic elution system Filta-Max xpress. Next, samples were purified during immunomagnetic separation process (IMS). Isolated cysts and oocysts were stained with FITC and DAPI and than the microscopic observation using an epifluorescence microscope was carried out. Results. Recovery of parasite protozoa in all contaminated water samples after 9-cycles elution process applied was mean 60.6% for Cryptosporidium oocysts and 36.1% for Giardia cysts. Studies on the environmental surface water samples showed the presence of both parasitic protozoa. Number of detected Giardia cysts ranged from 1.0/10 L up to 4.5/10 L in samples from Zegrzynski Lake and from 1.0/10 L up to 38.9 /10 L in samples from Vistula River. Cryptosporidium oocysts were present in 50% of samples from the Zegrzynski Lake and in 47.6% of samples from the Vistula River, and their number in both cases was similar and ranged from 0.5 up to 2.5 oocyst/10 L. The results show that applied procedure is appropriate for detection the presence of parasitic protosoan in water, but when water contains much amount of inorganic matter and suspended solids test method have to be modified like subsamples preparation and filtration process speed reduction. Conclusions. The applied method with the modification using Filta-Max xpress system can be useful for the routine monitoring of water. Detection of Cryptosporidium and Giardia in all samples of water taken from the intakes of surface water shows the possibility of transfering of the protozoan cysts into the water intended for the consumption, therefore the testing of Cryptosporidium and Giardia should be included into the monitoring of water.
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 2012, 63, 4
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metabolizm serotoniny u dzieci zarazonych Giardia intestinalis
    Autorzy:
    Lucinska, A
    Prusek, W.
    Galary, E.
    Podwysocka, M.
    Modrzycka, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/839378.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    choroby pasozytnicze
    parazytologia lekarska
    serotonina
    Giardia intestinalis
    dzieci
    pierwotniaki
    reakcje immunologiczne
    metabolizm
    giardioza
    inwazja pasozytnicza
    pasozyty czlowieka
    Opis:
    According to the data from the last 4 years, 3.8% of all diagnosed parasitoses are giardiases. During 5 years Giardia intestinalis was found in 24 chiidren hospitalized in the General Hospital of the Wrocław Voivodship: in 14 girls and 10 boys, 2-12 years old. The parasite was identified coproscopically and on the basis of clinical symptoms. Before the medication, the blood was taken from each child to determine the serotonin level and the (MAO) monoaminooxydaze, enzyme metabolizing serotonin acivity in the blood serum. The results were compared to the values obtained in the control group of 10 healthy children, 3-12 years old. III children were divided into two groups: first consisted of 12 children, 2-5 years old, second - of 12 children, 6-12 years old. In the first group 210% increase of the serotonin level in the blood and slight 32,4% increase of the MAO activity were observed in comparison with the control group. In the second group the serotonin level in the blood increased 221 % in comparison with the control, while the MAO actmty increased 45.1 %. In older children marced clinical symptoms could be observed, mainly malfunction of the alimentary tract, microelements shortage and anemia. The highest level of serotonin and MAO activity was found in the child with bile duet irritation. The reason for the increased level of serotonin and MAO activity can be pathogenic action of parasite's trophozoites and cysts on the jejunum mucosa and mobilization, proliferation and degranulation of tissue mastocytes. Serotonin, released from mastocytes, can be the cause of enhanced peristalsis and mucosa congestion.
    Źródło:
    Annals of Parasitology; 2000, 46, 1; 149-155
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zwierzeta ziemnowodne jako zrodlo zanieczyszczenia wody Cryptosporidium i Giardia
    Semi-aquatic animals as a source of water contamination with Cryptosporidium and Giardia
    Autorzy:
    Bajer, A.
    Bednarska, M.
    Paziewska, A.
    Romanowski, J.
    Sinski, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/840330.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pizmak
    bobr europejski
    zanieczyszczenia biologiczne
    Giardia
    pierwotniaki
    rezerwuary pasozytow
    pasozyty
    zwierzeta ziemnowodne
    zrodla zanieczyszczen
    Cryptosporidium
    zanieczyszczenia wod
    Źródło:
    Annals of Parasitology; 2008, 54, 4; 315-318
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wystepowanie Giardia intestinalis u psow domowych w Warszawie
    Prevalence of Giardia intestinalis in domestic dogs in Warsaw
    Autorzy:
    Zygner, W
    Jaros, D.
    Skowronska, M.
    Bogdanowicz-Kamirska, M.
    Wedrychowicz, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/839601.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    wystepowanie
    pasozyty zwierzat
    psy
    diagnostyka parazytologiczna
    choroby pasozytnicze
    Giardia intestinalis
    parazytologia
    konferencje
    giardioza
    Warszawa
    choroby zwierzat
    Warszawa konferencja
    Opis:
    Introduction. Giardia intestinalis is the most common intestinal protozoan parasite, which infects humans, dogs and other mammals throughout the world. So far eight genotypes of the parasite have been described of which four were found in dogs. Assemblages A-I and B infect either dogs or humans. Assemblages C and D occur only in dogs. The aim of the study was to determine the prevalence and genotypes of G. intestinalis in domestic dogs of Warsaw area. Material and methods. From October 2005 to March 2006 fecal samples were collected from 350 dogs and examined using light microscopy and PCR techniques. Results. 5.14% of dogs was found to be positive for G. intestinalis by microscopy and prevalence of 9.14% was found by PCR. The PCR amplicons were sequenced and the DNA sequences were compared with Giardia sequences in GeneBank database. The analysis revealed assemblage A-I in 1.71% of dogs, assemblage C in 1.14% and assemblage D in 6.28% of dogs in Warsaw. According to literature, the genotype A can infect humans however a role of dogs as a reservoir of human giardiosis in Poland is not known.
    Źródło:
    Annals of Parasitology; 2006, 52, 4; 311-315
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Inwazje Giardia intestinalis u szynszyli małej (Chinchilla lanigera)
    Parasitic infestation of Giardia intestinalis in long-tailed chinchilla (Chinchilla lanigera)
    Autorzy:
    Janczak, D.
    Barszcz, K.
    Cielecka, D.
    Golab, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/862038.pdf
    Data publikacji:
    2014
    Wydawca:
    Krajowa Izba Lekarsko-Weterynaryjna
    Tematy:
    zwierzeta futerkowe
    szynszyle
    pasozyty zwierzat
    pierwotniaki
    Giardia intestinalis
    cykl rozwojowy
    inwazja pasozytnicza
    diagnostyka
    leczenie
    profilaktyka
    zwalczanie pasozytow zwierzat
    Źródło:
    Życie Weterynaryjne; 2014, 89, 10
    0137-6810
    Pojawia się w:
    Życie Weterynaryjne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Laboratory animal models in parasitological research
    Autorzy:
    Kasprzak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/839828.pdf
    Data publikacji:
    1994
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    phylogenesis
    laboratory animal
    Giardia
    Trichomonas
    immunological process
    Protozoa
    taxonomy
    organism
    parasite
    Entamoeba histolytica
    Acanthamoeba
    man
    Naegleria
    parasitic infection
    Źródło:
    Annals of Parasitology; 1994, 40, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biologia, epidemiologia i diagnostyka chorobotworczych pierwotniakow przenoszonych za posrednictwem wody
    Biology, epidemiology and diagnostics of pathogenic waterborne protozoan parasites
    Autorzy:
    Leonska-Duniec, A
    Adamska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/838881.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    pasozyty jelitowe
    pierwotniaki
    Cryptosporidium
    Giardia
    Cyclospora cayetanensis
    Isospora belli
    Mikrosporidia
    biologia
    epidemiologia
    diagnostyka
    przenoszenie chorob
    woda
    zanieczyszczenia wod
    zanieczyszczenia mikrobiologiczne
    Źródło:
    Annals of Parasitology; 2010, 56, 2; 125-132
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of Giardia intestinalis DNA in environmental water and soil samples collected from the Pomerania Province and the Warmia-Masuria Province, Poland using real-time PCR and nested–PCR
    Autorzy:
    Lass, A.
    Szostakowska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/6172.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    detection
    Giardia intestinalis
    DNA
    environmental water
    soil sample
    Pomeranian region
    Warmia-Mazury region
    Polska
    real-time PCR method
    nested polymerase chain reaction
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biologia, epidemiologia i diagnostyka chorobotwórczych pierwotniaków przenoszonych za pośrednictwem wody
    Biology, epidemiology and diagnostics of pathogenic waterborne protozoan parasites
    Autorzy:
    Leońska-Duniec, A
    Adamska M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143426.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    czynniki chorobotworcze
    pasozyty jelitowe
    pierwotniaki
    Cryptosporidium
    Giardia
    Cyclospora cayetanensis
    Isospora belli
    Mikrosporidia
    biologia
    epidemiologia
    diagnostyka
    przenoszenie chorob
    woda
    zanieczyszczenia wod
    zanieczyszczenia mikrobiologiczne
    Opis:
    Cryptosporidium, Giardia intestinalis, Cyclospora cayetanensis, Isosopra belli and micropsoridia are the most important and common pathogens found in humans and many other species of vertebrates. In humans, mainly in immunocompromised patients, children, pregnant women and elderly people, they are the most frequently identified protozoan parasites causing gastrointestinal disease worldwide. These pathogens have several transmission routes, including anthroponotic and zoonotic transmission. What is more, in many cases of epidemics caused by mentioned pathogens the major cause of infection was contaminated with these organisms water and food. In spite of many existing regulations of clearing and making use of drinking water supplies and recreational water, cosmopolitan protozoan parasites are still the danger of public health. These organisms are responsible for many waterborne outbreaks worldwide. Light microscopy and immunofluorescence assay have been used to identify these organisms in most laboratories. However, these traditional techniques have major limitations in the specific diagnosis, these methods are not sensitive enough to detect cysts or oocysts in environmental samples, so the new molecular tools must be applied. Recently, PCR-based techniques have been developed for detection and genetic characterization of the different species and population variants of protozoan parasites is central to the prevention, surveillance and control of gastrointestinal diseases. In this review were characterized biology, epidemiology and the progress in technology for detection and surveillance of the most important waterborne protozoan parasites.
    Źródło:
    Wiadomości Parazytologiczne; 2010, 56, 2; 125-132
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Intestinal parasitic infections among mentally handicapped individuals in Alexandria, Egypt
    Autorzy:
    Shehata, A.I.
    Hassanein, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/5583.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    intestinal parasite
    Cryptosporidium
    microsporidia
    Giardia lamblia
    Dientamoeba fragilis
    Cyclospora cayetanensis
    Blastocystis hominis
    Entamoeba histolytica
    Entamoeba coli
    parasitic infection
    handicapped person
    Alexandria
    Egypt
    Opis:
    This cross-sectional study was carried to determine the prevalence of intestinal parasitic infections among mentally handicapped individuals in Alexandria, Egypt, in the period from December 2012 till November 2013. The study was conducted on 200 institutionalized and non-institutionalized mentally handicapped individuals. Fresh stool samples were subjected to different stains including; trichrome for detecting intestinal protozoa, modified acid fast stain for intestinal coccidia and quick hot gram chromotrope stain for Microsporidia. Also they were processed by Kato-Katz and formol ethyl acetate techniques for intestinal helminths. Additionally, blood samples were collected for measuring hemoglobin levels. Out of 200 mentally handicapped individuals, 87 (43.5%) were infected. The infection rates were 44.6% and 42.6% for non-institutionalized and institutionalized people, respectively. Regarding gender, 46.7% and 38.5% were reported for the males and females respectively. The most common parasites detected were: Cryptosporidium sp. (23.5%), microsporidia (15%), Giardia lamblia (8.5%), Dientamoeba fragilis (8%), Cyclospora cyatanensis (7.5%), Blastocystis hominis (6.5%), Entamoeba histolytica (5.5%) and Entamoeba coli (2.5%). Rates for Isospora belli and Enterobius vermicularis were estimated to be 1.5% for each, while lower rate was reported for Iodamoeba butschlii (1.0%). Prevalence of infections among mentally handicapped individuals are indications for several risk factors, including improper sanitary hygiene and illiteracy about personal hygiene. Therefore, frequent investigations, health care and medical intervention are needed.
    Źródło:
    Annals of Parasitology; 2015, 61, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiological and clinical picture of parasitic infections in the group of children and adolescents from north-east region of Poland
    Autorzy:
    Zukiewicz, M.
    Kaczmarski, M.
    Topczewska, M.
    Sidor, K.
    Tomaszewska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/837861.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic infection
    child
    adolescent
    Polska
    human disease
    epidemiology
    Ascaris lumbricoides
    Toxocara
    Giardia lamblia
    risk factor
    clinical symptom
    Strongyloides stercoralis
    Enterobius vermicularis
    ELISA test
    diagnosis
    Źródło:
    Annals of Parasitology; 2011, 57, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Distribution of Cryptosporidium and Giardia spp. in selected species of protected and game mammals from North-Eastern Poland
    Autorzy:
    Paziewska, A
    Bednarska, M.
    Nieweglowski, H.
    Karbowiak, G.
    Bajer, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/51999.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    beaver
    wolf
    red deer
    mammal
    Giardia
    Polska
    reservoir
    protozoan parasite
    parasite
    bison
    man
    genotype
    parasitology
    North-Eastern Poland
    roe deer
    Cryptosporidium
    intestinal parasite
    Opis:
    Cryptosporidium spp. and Giardia spp. are wide-spread pathogens of humans and many species of mammals. The ways of transmission are very complex and diffi cult to defi ne. Both parasites occur in similar environments and share a broad host range. However, in Poland there is still little known about the epidemiology of these parasites due to the paucity of data on human cases and only few studies in wildlife. The aim of our study was to determine the distribution of two intestinal protozoa in a few species of protected and game mammals in North-Eastern Poland. Additionally, we wanted to compare prevalence and abundance of these parasites between wild and farm animals, and to determine the species/genotypes of Cryptosporidium. Fecal samples collected from protected species (European beaver – 22, grey wolf – 14, European bison – 55, Polish Konik (horse) – 5) and game mammals (red deer – 52, roe deer – 22, boar – 5) were examined by IFA. We also studied a group of samples collected from farm animals: beaver – 30, red deer – 66, Polish konik – 5. Cryptosporidium oocysts were identifi ed in 5 of 7 studied animal species (prevalence from 9% in roe deer to 36% in wolves), Giardia cysts in 4 of 6 studied species (prevalence from 1.7% in red deer to 7.7% in European beaver). Sequencing analysis of COWP gene fragment revealed that 5 Cryptosporidium isolates from wolves were C. parvum genotype 2 (zoonotic). The results show the important role of examined species in maintaining the natural sources of Cryptosporidium spp. and Giardia spp. infections in the environment.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Selected aspects of multifocal invasions
    Autorzy:
    Kwasniewska, J.
    Kurnatowska, A.
    Kurnatowski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/840156.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Giardia
    multifocal invasion
    Trichomonas
    Mucor
    fungi
    Aspergillus
    Entamoeba
    Fusarium
    Geotrichum
    parasite
    Enterobius
    Cryptococcus
    Acanthamoeba
    multifocal infection
    Rhizopus
    life cycle
    Naegleria
    Candida
    Toxocara
    Echinococcus
    Paecilomyces
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epidemiological and clinical picture of parasitic infections in the group of children and adolescents from north-east region of Poland
    Autorzy:
    Żukiewicz, M.
    Kaczmarski, M.
    Topczewska, M.
    Sidor, K.
    Tomaszewska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143366.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic infection
    child
    adolescent
    Polska
    human disease
    epidemiology
    Ascaris lumbricoides
    Toxocara
    Giardia lamblia
    risk factor
    clinical symptom
    Strongyloides stercoralis
    Enterobius vermicularis
    ELISA test
    diagnosis
    Opis:
    The epidemiological and clinical purview of parasite diseases is constantly evolving, mainly due to the changes in human behavior and our environment. The aim of this study was frequency analysis of certain parasite infections, risk factors and clinical symptoms in children and adolescents of north-eastern Poland. 120 children were tested due to the symptoms resembling parasite infection from December 2008 to May 2009. 90 patients (the tested group) aged from 5 months to 18 years were found to be infected with one or more than one of the following parasites: Ascaris lumbricoides, Giardia lamblia, Toxocara spp., Strongyloides stercoralis or Enterobius vermicularis. 30 children with no infections found were the control group. Parasites were found by means of microscope decantation stool tests, RIDA Quick Giardia stool test and ELISA blood test (Toxocara IgG). The patients’ parents filled out the survey concerning the probability factors of infection and the ailments that were the cause of parasitical diagnosis. High percentage of either isolated or co-invasive parasite infections was ascertained in the tested group – 75%. The prevalence of ascariasis was 55.83%, toxocariasis – 16.67%, giardiasis – 12.5%, strongyloidiasis – 5.83% and of enterobiasis – 3.33%. The statistical significance of higher dirty hands–mouth contact frequency and more frequent presence of domestic animals in the tested group than in the control group was ascertained. A highly important correlation between the infection and the occurrence of symptoms from at least 3 various systems was ascertained. The presence of habits involving dirty hands – mouth contact and having domestic animals are significant risk factors of parasite infections. The presence of symptoms from at least 3 systems should compel to parasite infection diagnosis with proper differential diagnosis.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 3; 179-187
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie Entamoeba histolytica i innych pierwotniaków jelitowych wśród ludności Wielkopolski w okresie ostatnich 30 lat
    Prevalence of Entamoeba histolytica and other intestinal protozoa among the inhabitants of Poznan province in the period of 30 years
    Autorzy:
    Kasprzak, W.
    Mazur, T.
    Karlewiczowa, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/2152477.pdf
    Data publikacji:
    1989
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia lekarska
    pasozyty czlowieka
    pasozyty jelitowe
    pierwotniaki
    Entamoeba histolytica
    Entamoeba hartmanni
    Entamoeba nana
    Entamoeba coli
    Giardia intestinalis
    Dientamoeba fragilis
    wystepowanie
    czestosc wystepowania
    ludnosc
    Wielkopolska
    lata 1958-1986
    Opis:
    The aim of the study was to present the past and actual prevalence of Entamoeba histolytica and other intestinal protozoa in Poznań Province. Although in Poland there are only few accurate data concerning the prevalence of intestinal protozoa, the results of some studies were found reliable. In a survey conducted in 1958 in an orphanage in Poznań (Kasprzak and Karlewiczowa) the intestinal protozoa were found with high prevalence (Table 1). After six years this prevalence decreased considerably (Karlewiczowa and Kasprzak, 1964). Because at the same time such a decrease in intestinal protozoa prevalence, especially that in Entamoeba coli, was observed in the whole population of the Poznań Province, long-term surveys were undertaken. The diagnosing of intestinal protozoa in faeces of 4165 persons, performed at more points in time in the period of 1963 to 1986 in the Poznań Province, were conducted by the same highly skilled staff. Decreasing rates of infection were seen for each amoeba species at nearly each point in time, particularly in the first eight years of observation (Table 2). By using the "amoebic index" and "amoebic prevalence rate" in assessing epidemiological factors (acc. WHO, 1969), the considerable reduction in both indices should be accounted for the improvement of the sanitation and socio-economic status in our country. After the Second World War some Polish authors expressed the fear that after returning to the country, the Polish soldiers who had fought in the endemic regions of invasive amoebiasis, infected with E. histolytica, would constitute sources for spreading amoebiasis and that the disease would be a nucleus of a new, hitherto unknown, social calamity in Poland. Fortunately, the fear did not come true. Although in this country the infection with amoebae, also with E. histolytica, decreased considerably after several years, unfortunately, some opinions still prevail, being the source of an amoebo-mania in the medical staff and corresponding amoebophobia in the patients.
    Źródło:
    Wiadomości Parazytologiczne; 1989, 35, 6; 535-545
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Giardia prevalence in wild cervids in Poland
    Autorzy:
    Majewska, A.C.
    Solarczyk, P.
    Moskwa, B.
    Cabaj, W.
    Jankowska, W.
    Nowosad, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/6186.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Giardia
    cyst
    prevalence
    wild animal
    cervid
    Dama dama
    fallow deer
    red deer
    Cervus elaphus
    roe deer
    Capreolus capreolus
    moose
    Alces alces
    fecal sample
    cervid zob.tez deer
    Polska
    Źródło:
    Annals of Parasitology; 2012, 58, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Występowanie Giardia intestinalis w środowiskach rodzinnych w świetle badań epidemiologicznych
    The occurrence of Giardia intestinalis in family environments in the light of epidemiological studies
    Autorzy:
    Głowniak, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2152556.pdf
    Data publikacji:
    1989
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parazytologia lekarska
    choroby pasozytnicze
    giardioza
    pasozyty czlowieka
    pasozyty przewodu pokarmowego
    Giardia intestinalis
    wystepowanie
    srodowisko rodzinne
    rodziny miejskie
    rodziny wiejskie
    wiek czlowieka
    inwazja pasozytnicza
    ekstensywnosc inwazji
    badania epidemiologiczne
    Polska Poludniowo-Wschodnia
    Opis:
    Coproscopy parasitological studlies were carried out in family environments. Samples of feces were taken three times from 1728 persons (400 familes) from urban and rural environments in south-eastern regions of Poland. The general extensity of giardiasis amounted to 39.6% and was higher in urban families (average 41.3%) than in those inhabiting rural areas (average 36.6%). The giardiasis dominated in children from 1 to 4 years (average more than 78% infected), especially in urban areas (extensity of infection 80.1%). It was also frequent in persons of male sex.
    Źródło:
    Wiadomości Parazytologiczne; 1989, 35, 1; 25-34
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-72 z 72

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