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Wyświetlanie 1-15 z 31
Tytuł:
A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences
Autorzy:
Kotłowski, Roman
Powiązania:
https://bibliotekanauki.pl/articles/1039112.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Mycobacterium tuberculosis complex
PGRS
genotyping
Opis:
Tuberculosis is one of the leading infectious diseases. In this work, a new genotyping method of Mycobacterium tuberculosis (Mtb) complex strain is presented. 27 Mtb genomes were analyzed for the presence of length polymorphism within polymorphic GC-rich gene sequences. Four genes, Rv3345c, Rv3507, Rv0747 and Rv3511, showing variation in length depending on the Mtb strain were selected for designing primer sequences flanking variable regions for the PCR method. Identification of 16 genotypes among 27 analyzed genomes demonstrated usefulness of our genotyping method in differentiation of Mtb genomes based on sequence polymorphism in the four PGRS genes.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 317-322
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First record of Giardia assemblage D infection in farmed raccoon dogs (Nyctereutes procyonoides)
Autorzy:
Solarczyk, Piotr
Majewska, Anna C.
Jędrzejewski, Szymon
Górecki, Marcin T.
Nowicki, Sławomir
Przysiecki, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/989740.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
giardia
molecular genotyping
raccoon dog
Opis:
The presence of Giardia genotypes was investigated in 18 raccoon dogs (Nyctereutes procyonoides) and 80 red foxes (Vulpes vulpes) on one farm. To demonstrate Giardia cysts, fresh and trichrome stained smears were microscopically screened. Two molecular markers were used for Giardia genotyping: a fragment of the beta-giardin gene and a fragment of the glutamate dehydrogenase gene. All faecal samples obtained from red foxes were negative. Giardia cysts were identified only in 2 of the 18 raccoon dogs. The result of genotyping and phylogenetic analysis showed that the G. duodenalis from both raccoon dogs belonged to the D assemblage. This finding of a new animal reservoir of G. duodenalis canids-specific genotypes is important in order to eliminate the risk of infecting other animals bred for fur. Further molecular analyses of Giardia isolates in raccoon dogs are required. The present study represents the first contribution to knowledge of G. duodenalis genotypes in raccoon dogs.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.)
Autorzy:
Kamel, Katarzyna
Kroc, Magdalena
Święcicki, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1039001.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
HRM
molecular markers
genotyping
narrow-leafed lupin
Opis:
Sequence tagged site (STS) markers are valuable tools for genetic and physical mapping that can be successfully used in comparative analyses among related species. Current challenges for molecular markers genotyping in plants include the lack of fast, sensitive and inexpensive methods suitable for sequence variant detection. In contrast, high resolution melting (HRM) is a simple and high-throughput assay, which has been widely applied in sequence polymorphism identification as well as in the studies of genetic variability and genotyping. The present study is the first attempt to use the HRM analysis to genotype STS markers in narrow-leafed lupin (Lupinus angustifolius L.). The sensitivity and utility of this method was confirmed by the sequence polymorphism detection based on melting curve profiles in the parental genotypes and progeny of the narrow-leafed lupin mapping population. Application of different approaches, including amplicon size and a simulated heterozygote analysis, has allowed for successful genetic mapping of 16 new STS markers in the narrow-leafed lupin genome.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 533-540
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of Toxoplasma gondii and Neospora caninum in red deer from Central Italy
Autorzy:
Rocchigiani, Guido
Nardoni, Simona
D’Ascenzi, Carlo
Nicoloso, Sandro
Picciolli, Federico
Papini, Roberto Amerigo
Mancianti, Francesca
Powiązania:
https://bibliotekanauki.pl/articles/989655.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
cervus elaphus
tissue apicomplexa
ifat
pcr
genotyping
Opis:
Neospora caninum and Toxoplasma gondii are cosmopolite protozoan parasites impacting on human and animal health. In particular, T. gondii commonly infects human beings and all warm-blooded animals, while N. caninum is responsible for bovine abortion and neuromuscular disease in dogs. The aim of the presented survey was to evaluate the occurrence and prevalence of these parasites in the most numerous Italian red deer population. The sera of 60 red deer (Cervus elaphus) inhabiting Central Italy (43°56’N 10°55’E) and killed by selective hunting were examined using an indirect fluorescent antibody test (IFAT) for both N. caninum and T. gondii antibodies. White blood cells (buffy coat) were also checked by PCR and T. gondii DNA was genotyped. Thirteen out of 60 sera (22%) scored positive for Toxoplasma, 17 samples (28%) were Neospora positive. Coinfection was recorded in 5 cases (8%). T. gondii (genotype II) and N. caninum DNA was detected in one and 3 samples of buffy coat, respectively. The presented study is the first to examine the occurrence of these parasites in the most numerous red deer Italian population, confirming this animal species as carrier of the investigated pathogens. These animals spread near human settlements, co-inhabiting with final hosts of T. gondii and N. caninum and could contribute to their transmission to domestic ruminants and humans. In particular, the seroprevalence value for N. caninum was the highest among European records.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowoczesne metody genotypowania DArT i GBS w hodowli gatunków roślin użytkowych
Modern methods of genotyping DArT and GBS in agriculture important crops
Autorzy:
Pachota, Katarzyna
Niedziela, Agnieszka
Orłowska, Renata
Bednarek, Piotr T.
Powiązania:
https://bibliotekanauki.pl/articles/2199126.pdf
Data publikacji:
2016-06-30
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
genotypowanie
DArT
Technika Diversity Array
GBS
SNP
genotyping
Diversity Array Technology
Genotyping-by-sequencing
Opis:
Rozwój nowoczesnych metod genotypowania sprawił, że konieczne staje się ich zaprezentowanie i pokazanie potencjału, jaki niosą generowane przez nie markery DNA dla badań nad roślinami. Niniejsza praca została poświęcona omówieniu markerów DArT i SNP, przy czym w ostatnim przypadku skupiono się na markerach wykorzystujących sekwencjonowanie nowej generacji. Przedstawiono metody uzyskania w/w markerów oraz ich zastosowania w genetyce roślin użytkowych. Nacisk położono na identyfikację markerów cech użytkowych w połączeniu z mapowaniem cech ilościowych, mapowaniem asocjacyjnym i selekcją genomową.
Advances in modern genotyping methods make it necessary to present them and their value for plant research. Thus, the review is dedicated to describing technologies allowing evaluation of high throughput markers such as DArTs or SNPs based on new generation sequencing approach. Various applications of these markers in plant research are discussed. Particular emphasis was put on the possibilities of using them for the identification of markers for agronomical traits using either QTL or association mapping methods. The opportunity of using them in genomic selection was also discussed.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2016, 279; 3-18
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of local cattle
Autorzy:
Kukučková, Veronika
Moravčíková, Nina
Curik, Ino
Simčič, Mojca
Mészáros, Gábor
Kasarda, Radovan
Powiązania:
https://bibliotekanauki.pl/articles/1038372.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
admixture
clustering
gene flow
genotyping array
Pinzgau cattle
Opis:
The Slovak Pinzgau breed faces the bottleneck effect and the loss of diversity due to unequal use of founders and a significant population decline. Further population size reduction can lead to serious problems. Information obtained here and in other studies from high-throughput genotyping of 179 individuals was used to characterise genetic diversity and differentiation of Slovak Pinzgau, Austrian Pinzgau, Cika and Piedmontese cattle by Bayesian clustering algorithm. A gene flow network for the clusters estimated from admixture results was produced. The low estimate of genetic differentiation (FST) in Pinzgau cattle populations indicated that differentiation among these populations is low, particularly owing to a common historical origin and high gene flow. Changes in the log marginal likelihood indicated Austrian Pinzgau as the most similar breed to Slovak Pinzgau. All populations except the Piedmontese one displayed two ways of gene flow among populations, indicating that Piedmontese cattle was involved in producing of the analysed breeds while these breeds were not involved in creation of Piedmontese. Genetic evaluation represents an important tool in breeding and cattle selection. It is more strategically important than ever to preserve as much of the livestock diversity as possible, to ensure a prompt and proper response to the needs of future generations. Information provided by the fine-scale genetic characterization of this study clearly shows that there is a difference in genetic composition of Slovak and Austrian populations, as well as the Cika and Piedmontese cattle. Despite its population size, the Slovak Pinzgau cattle have a potential to serve as a basic gene reserve of this breed, with European and world-wide importance.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 421-424
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Host Range of Cyclospora Species: Zoonotic Implication
Autorzy:
Solarczyk, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/2041956.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Cyclospora
animals
zoonosis
epidemiology
genotyping
next-generation sequencing
Opis:
Cyclospora is an intracellular, gastrointestinal parasite found in birds and mammals worldwide. Limited accessibility of the protozoan for experimental use, scarcity, genome heterogeneity of the isolates and narrow panel of molecular markers hamper zoonotic investigations. One of the significant limitation in zoonotic studies is the lack of precise molecular tools that would be useful in linking animal vectors as a source of human infection. Strong and convincing evidence of zoonotic features will be achieved through proper typing of Cyclospora spp. taxonomic units (e.g. species or genotypes) in animal reservoirs. The most promising method that can be employ for zoonotic surveys is next-generation sequencing.
Źródło:
Acta Protozoologica; 2021, 60; 13-20
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Association Analysis of GDF5 and Contributing Factors in Developmental Dysplasia of the Hip in Infants
Autorzy:
Harsanyi, Stefan
Zamborsky, Radoslav
Krajciova, Lubica
Bohmer, Daniel
Kokavec, Milan
Danisovic, Lubos
Powiązania:
https://bibliotekanauki.pl/articles/28409369.pdf
Data publikacji:
2021-10-25
Wydawca:
Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
Tematy:
DDH
hip joint
genotyping
GDF5
rs143383
Opis:
Background. Developmental dysplasia of the hip (DDH) is a developmental disorder which is reported to be associated with hip instability. When untreated, it can lead to irreversible joint damage. DDH is known to be a multifactorial disease involving genetic, mechanical and environmental factors. The greatest causative potential is attributed to the genetic component. Growth Differentiation Factor 5 (GDF5) is among the most studied genes associated with processes of regeneration and maintenance of joints. The aim of this work was to analyse the association of SNP rs143383 in the GDF5 gene and the occurrence of DDH, along with association with various contributing factors in the Caucasian population. Material and methods. A total of 118 samples were analysed for the presence of the mutation. DNA was isolated from all individuals from peripheral blood. SNP rs143383 in the GDF5 gene was genotyped using the TaqMan assay. A standard chi-square test was used to compare allele and genotype distributions in patients and healthy controls. Results. The association analysis of genotypes of DDH and rs143383 revealed a significant association. Also, the association of GDF5 and selected contributing factors was statistically significant in female gender (p=0.002), family history (p<0.001), count of pregnancy (p=0.009), laterality of hip involvement and initial US examination. Conclusions. 1. The results indicate an important effect of rs143383 polymorphism in the GDF5 gene on DDH development. 2. However, our results also suggest that rs143383 is not the only contributing factor in the genetic component of DDH.
Źródło:
Ortopedia Traumatologia Rehabilitacja; 2021, 23(5); 335-339
1509-3492
2084-4336
Pojawia się w:
Ortopedia Traumatologia Rehabilitacja
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
S1 gene based phylogeny of Israel variant-2 infectious bronchitis virus isolated in Turkey in a five year period
Autorzy:
Müştak, İ.B.
Müştak, H.K.
Bilgen, N.
Powiązania:
https://bibliotekanauki.pl/articles/16539076.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
broilers
genotyping
infectious bronchitis virus
molecular epidemiology
Israel variant 2
Opis:
Infectious bronchitis (IB) is an important disease that causes severe economic loses in the poultry industry worldwide. Furthermore, the spread of new variants poses a challenge for diagnosis and control of the disease. This study investigated the situation of infectious bronchitis virus (IBV), specifically the Israel variant-2 (IS var-2) also known as GI-23 genotype, in Turkey. Between 2014 and 2019, 214 flocks vaccinated against H120 from Marmara, Western Black Sea, and Inner Anatolia were examined, with 127 (59.3%) flocks testing positive for IBV, of which 92 (72.4%) were positive for IS var-2. Of the latter samples, 60 were randomly selected and subjected to full S1 gene sequencing. The analysis indicated that the field strain in Turkey was located on the same branch as the GI-23 genotype, which is one of the most frequently observed wild-type cluster found in the Middle East. The DNA similarities between the GI-23 isolates from 2014 to 2019 were 99%. In conclusion, the IS var-2 genotype has been circulating in broiler flocks in Turkey. It is recommended that establishing the vaccine strategy it should be considered the current circulating strains for the prevention and control of the disease among poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 45-50
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxoplasma gondii infection in selected species of free-living animals in Poland
Autorzy:
Sroka, J.
Karamon, J.
Wójcik-Fatla, A.
Dutkiewicz, J.
Bilska-Zając, E.
Zając, V.
Piotrowska, W.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2085130.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Toxoplasma gondii
nested PCR
genotyping
free-living animals
Opis:
Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotype-specific human papillomavirus detection in cervical smears
Autorzy:
Szostek, Slawa
Klimek, Malgorzata
Zawilinska, Barbara
Kosz-Vnenchak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1040671.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
human papillomavirus
cervical carcinoma
HPV genotyping
squamous intraepithelial lesions
Opis:
Human papillomavirus (HPV) is widely accepted as a causative agent of cervical cancer. The distribution and prevalence of HPV types depend on geographic region and demographic factors. The aim of this study was to investigate the relationship between the presence of various HPV types and the outcome of cytological examination. Cervical smears were obtained from 125 women from southern Poland: low grade squamous intraepithelial lesions (LSIL) - 44, high grade squamous intraepithelial lesions (HSIL) - 12, cervical carcinoma - 27 and 42 women without abnormality in cytology as a control group. DNA was extracted from the smears and broad-spectrum HPV DNA amplification and genotyping was performed with the SPF 10 primer set and reverse hybridisation line probe assay (INNO-LiPA HPV Genotyping, Innogenetics). HPV DNA was detected in approximately 72% cases, more frequently in women with squamous intraepithelial lesions and cervical carcinoma than in the control group (P < 0.0005). The most frequent type found was HPV 16 (37%), followed by HPV 51 (28%) and HPV 52 (17%). A single HPV type was detected in 51% positive cases, more frequently in cervical cancer specimens. Multiple HPV infection was dominant in women with LSIL and normal cytology. Prevalence of HPV 16 increased with the severity of cervical smear abnormality. For women HPV 16 positive, the relative risk (odds ratio) of the occurrence of HSIL and cervical cancer versus LSIL was 14.4 (95% CI, 3.0-69.2; P=0.001) and 49.4 (95% CI, 6.5-372.8; P < 0.001), respectively. Genotyping of HPV will allow better classification of women with cervical abnormalities into different risk groups and could be useful in therapy.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 687-692
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
Autorzy:
Krawczyk, Beata
Kur, Józef
Stojowska-Swędrzyńska, Karolina
Śpibida, Marta
Powiązania:
https://bibliotekanauki.pl/articles/1038839.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
rapid methods
molecular epidemiology
genotyping
microbial phylogenetics
PCR (polymerase chain reaction)
Opis:
A significant number of DNA-based techniques has been introduced into the field of microorganisms' characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before DNA amplification by PCR, known as Ligation-Mediated PCR methods (LM PCR), have been successfully applied for the typing of microorganisms below the species level. These molecular methods include: Amplified Fragment Length Polymorphism (AFLP), Amplification of DNA fragments Surrounding Rare Restriction Sites (ADSRRS), PCR Melting Profiles (PCR MP), Ligation Mediated PCR/Shifter (LM PCR/Shifter), Infrequent-Restriction-Site Amplification (IRS PCR), double digestion Ligation Mediated Suppression PCR (ddLMS PCR). These techniques are now applied more and more often because they involve less time, are comparably inexpensive, and require only standard lab equipment. Here, we present a general review of this group of methods showing their possibilities and limitations. We also identify questions and propose solutions which may be helpful in choosing a particular LM PCR method for the achievement of the required goal.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 39-52
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wybrane elementy nowoczesnych rozwiązań wpływające na skuteczność programów hodowlanych
Up-to-date solutions in modern plant breeding programs
Autorzy:
Niedziela, Agnieszka
Jarska, Weronika
Bednarek, Piotr T.
Powiązania:
https://bibliotekanauki.pl/articles/2198602.pdf
Data publikacji:
2015-03-31
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
genotypowanie
fenotypowanie
mapowanie
cechy ilościowe
selekcja
genotyping
phenotyping
mapping
QTL
selection
Opis:
Postęp hodowli roślin uzależniony jest od technologii pozwalających na identyfikację markerów cech ilościowych takich jak profilowanie DNA, czy też ich fenotypowanie. Coraz większe znaczenie zaczynają odgrywać metody statystyczne wykorzystywane w mapowaniu asocjacyjnym i selekcji genomowej. W ostatnich latach obserwuje się zmianę podejścia do selekcji. Zamiast wykorzystywać pojedyncze markery cech poszukuje się wielu markerów tych cech lub też stosuje całą dostępną pulę markerów opisujących daną populację dla potrzeb selekcji. Większość ze stosowanych metod selekcji związanych jest z wykorzystaniem wydajnych technologii markerowych, natomiast problemem pozostaje ograniczona dostępność i wysokie koszty fenotypowania roślin. W pracy przedstawiono wybrane elementy nowoczesnych rozwiązań mogących wpływać na skuteczność oraz czas realizacji programów hodowlanych.
Advances in plant breeding depend on technologies that allow the identification of markers linked to agronomical traits that could be achieved via genotyping and phenotyping. There is a growing interest in the application of statistical methods developed for association mapping and genomic selection. In the last few years, a change in attitude towards plant selection is being observed. Instead of the tendency of identifying single markers for some traits the attention is focused on the evaluation of numerous markers from the QTL regions or the whole available pool of markers is utilized in a selection process. Most of the modern approaches are based on the efficient marker technologies whereas phenotyping on a large scale, is still a problem. This paper is devoted to the aspects affecting efficiency of numerous stages of selection used in plant breeding and involving advances in molecular biology.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2015, 275; 3-15
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Database system for supporting innovative methodology for pine protein genotyping
System bazodanowy wspomagający innowacyjną metodykę genotypowania białkowego sosny zwyczajnej
Autorzy:
Nowakowski, K.
Nowak, P. J.
Tomczak, R. J.
Kujawa, R.
Przybylski, G.
Powiązania:
https://bibliotekanauki.pl/articles/336918.pdf
Data publikacji:
2015
Wydawca:
Sieć Badawcza Łukasiewicz - Przemysłowy Instytut Maszyn Rolniczych
Tematy:
Scots pine
Pinus sylvestris L.
database system
genotyping
sosna zwyczajna
system bazodanowy
genotypowanie
Opis:
Project “Database system for supporting innovative methodology for pine protein genotyping” was aimed to create a system of representing information about the trees used in selective breeding carried out by Polish State Forests. Presented information includes data on general characteristics, such as geographic location and special features, such as genetic variation. The project is based on Microsoft technologies: - ASP.NET to build a website with all the functionality - SQL Server 2008 responsible for database platform.
Projekt pt. „System bazowy wspomagający innowacyjna metodykę genotypowania białkowego sosny zwyczajnej" miał na celu wytworzenie systemu prezentacji informacji na temat drzew wykorzystywanych w hodowli selekcyjnej prowadzonej przez Lasy Państwowe. Przedstawiona informacja zawiera dane na temat cech ogólnych, takich jak położenie geograficzne i cech specjalistycznych, jak zmienność genetyczna. Projekt powstał w oparciu o technologie firmy Microsoft: - ASP.NET stworzenie strony wraz z cała funkcjonalnością - SQL Server 2008 program odpowiadający za platformę bazodanową.
Źródło:
Journal of Research and Applications in Agricultural Engineering; 2015, 60, 1; 55-58
1642-686X
2719-423X
Pojawia się w:
Journal of Research and Applications in Agricultural Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of the factor V Leiden mutation in patients susceptible to venous thromboembolism
Częstość występowania mutacji czynnika V Leiden u pacjentów ze skłonnością do żylnej choroby zakrzepowo-zatorowej
Autorzy:
Mitrus, J.M.
Wierszyło, E.
Powiązania:
https://bibliotekanauki.pl/articles/2048961.pdf
Data publikacji:
2020
Wydawca:
Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
Tematy:
hypercoagulability
Leiden mutation
genotyping techniques
Real-Time PCR
nadkrzepliwość
mutacja Leiden
genotypowanie
Opis:
Background. A tendency to venous thromboembolism is otherwise called hypercoagulability or thrombophilia. This disease can be acquired or have a genetic background, and may lead to pulmonary embolism. The basis for analysis and selection of treatment is genetic diagnosis, which detects the G1691A mutation in the factor V gene (factor V Leiden) – the best known congenital thrombophilia marker. Material and methods. The study was carried out in the years 2015-2017 on samples taken from patients (462 men and 1284 women) with a tendency to venous thromboembolism. Real-Time PCR was used to detect G1691A mutation in factor V gene. The analyses were performed in the Hematology Laboratory of the Center of Laboratory Medicine at the Medical University of Gdańsk. Results. Significant differences in the frequency of Leiden mutation were shown. This mutation predominated in men (25%), while in women G1691A mutation was detected with a 15% frequency (p=0.04). All possible genotypes were found among the subjects and the percentage of heterozygotes and homozygotes in both genders was similar. Conclusions. Congenital t hrombophilia a ssociated w ith G1691A mutation of f actor V Leiden gene was found to be more common in men than in women. All possible genotypes were determined in the pool of test subjects. The mutation was most frequently detected in patients between 30 and 40 years of age, and rarely after 70 years of age.
Wprowadzenie. Nadkrzepliwość (trombofilia) jest to skłonność do żylnej choroby zakrzepowozatorowej. Choroba ta może być nabyta lub mieć podłoże genetyczne i może prowadzić do zatorowości płucnej. Podstawą analizy i doboru leczenia jest diagnostyka genetyczna. Celem tej diagnostyki jest wykrycie mutacji G1691A w genie czynnika V (czynnik V Leiden) – najlepiej poznanego markera trombofilii wrodzonej. Materiał i metody. Badania zostały przeprowadzone w latach 2015-2017 na próbkach pobranych od pacjentów (462 mężczyzn i 1284 kobiet) ze skłonnością do żylnej choroby zakrzepowozatorowej. W celu wykrycia mutacji G1691A w genie czynnika V zastosowano metodę Real-Time PCR. Analizy zostały wykonane w Laboratorium Hematologii Uniwersyteckiego Centrum Medycyny Laboratoryjnej przy Gdańskim Uniwersytecie Medycznym. Wyniki. Wykazano istotne statystycznie różnice w częstości występowania mutacji Leiden. Mutację częściej występowała w grupie mężczyzn (25%), zaś u kobiet mutację G1691A wykrywano z częstością 15% (p=0,04). Wśród osób badanych stwierdzono wszystkie możliwe genotypy, a procentowy udział heterozygot i homozygot u obu płci był zbliżony. Wnioski. Trombofilia wrodzona związana z mutacją G1691A w genie czynnika V Leiden częściej występowała u mężczyzn niż kobiet. W puli osób poddanych testom oznaczono wszystkie możliwe genotypy. Mutację najczęściej wykrywano u pacjentów w przedziale wiekowym 30-40 lat, a najrzadziej po 70 roku życia.
Źródło:
Health Problems of Civilization; 2020, 14, 2; 83-93
2353-6942
2354-0265
Pojawia się w:
Health Problems of Civilization
Dostawca treści:
Biblioteka Nauki
Artykuł
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