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    Wyświetlanie 1-7 z 7
    Tytuł:
    Anti-inflammatory effect on genes expression after four days of Qigong training in peripheral mononuclear blood cells in healthy women
    Autorzy:
    Półrola, P.
    Wilk-Frańczuk, M.
    Wilczyński, J.
    Nowak-Starz, G.
    Góral-Półrola, J.
    Chrusciński, G.
    Bonisławska, I.
    Pedrycz, A.
    Żychowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081731.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    qigong
    genes expression
    heat shock protein
    inflammation
    Opis:
    Introduction. Some studies have shown the influence of Qigong on gene expression in different cells, but there is little data associated with the influence of this kind of therapy on genes expression in pheripheral monocellucar blood cells. Objective. The aim of this study was to evaluate changes in the expression of genes associated with cellular stress response in peripheral mononuclear blood cells (PMBC) in healthy women. Materials and method. The experiment took place at the Japanese Martial Arts Centre “DOJO” in Stara Wieś, Poland, conducted over the course of a 4-day qigong training session. To evaluate the genes effect of this training, blood samples were taken before and after the training period. This experiment involved 20 healthy women (aged 56.2±9.01, body height 164.8±6.5 and mass 65.5±8.2). To determine the expression of HSF-1, HSPA1A, NF-kB, IL10 and CCL2 mRNA, 3 ml of venous blood was collected. The blood samples were placed in tubes allowing for separation (BD Vacutainer CPTTM) before and after the 4 days of qigong training. Isolated PMBC were used to determine gene expression using real-time qRT-PCR (quantitative reverse transcription polymerase chain reaction). Results. Significant decreases in NF-kB and CCL2 mRNA and increases in IL10, HSF1 and HSPA1A m-RNA were detected after 4 days of qigong training. The obtained findings suggest that qigong caused a reduction in the inflammatory and intensified anti-inflammatory gene expression, as well as a higher expression of HSF-1 and HSPA1A. Conclusion. The adaptive response to qigong training was similar to the adaptive response to physical activity and was detected through gene expression in PMBC. Furthermore, this kind of training is especially indicated for women because of their higher susceptibility to psychosocial stress when compared to men.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 329-333
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An evaluation of the genetic conditioning of evoking pain
    Autorzy:
    Pacian, A.B.
    Kocki, J.
    Pacian, J.
    Kaczoruk, M.
    Bylina, M.
    Kaczor-Szkodny, P.
    Galińska, E.M.
    Kulik, T.
    Panasiuk, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085535.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    genetics
    chronic pain
    genes expression
    DRD1 gene
    molecular research
    Opis:
    Introduction and objective. Pain is an integral element of the pathogenic process and sometimes determines its course. Disorders in pain sensation, as well as its lack, the pain threshold, and variability in sensation of the same pain stimuli as more or less intensive by different persons, may be genetically conditioned. The aim of the study is to examine genes in pathogenesis of chronic pain. Materials and method. The study was conducted in a specially selected group of 31 persons: study group – 20 patients with chronic pain, and control group – 11 healthy individuals who did not experience pain. The control group of 11 healthy persons, compared with the study group, was the catalyst for determining the relative quantification (RQ) of gene expression. Biological material in the form of venous blood was collected from the study participants into the tubes containing anticoagulant EDTA KE/2.7 ml (ethylenediaminetetraacetic acid), preventing extracorporeal blood clotting. Results. Analysis of expression of the examined genes showed over-expression of the DRD1 gene in patients experiencing chronic pain, which means that in these patients an increased number of dopamine D1 receptors encoded by this gene should be expected. The dopamine D1 receptor is a G-protein-coupled receptor which regulates (stimulates or inhibits) adenyl cyclase – the enzyme responsible for synthesis of cyclic AMP (cAMP). An increase in the concentration of cAMP in neurons enhances the sensation of pain. Conclusions. The genes (DRD1, COMT, OPRK1, HCN2) have a significant role in the pathogenesis of chronic pain in various diseases; they can also influence the perception of pain. Knowledge of these genes can contribute to the development of effective methods of combating pain.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 2; 274-278
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Revealing the core transcriptome modulating plant growth phase in Arabidopsis thalianaby RNA sequencingand coexpression analysis of the FHY3 FAR1 mutant
    Autorzy:
    Wang, W.
    Zhao, G.
    Wu, S.
    Hua, W.
    Zhang, T.
    Ruan, R.
    Cheng, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2130692.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    weekly transcriptome analysis
    plant development
    different expression genes
    (DEGs)
    immune response
    Opis:
    Plants must continually calibrate their growth in response to the environment throughout their whole life cycle. Revealing the regularity of plant early growth and development is of great significance to plant genetic modification. It was previously demonstrated that loss of two key light signaling transcription factors, FHY3 and FAR1, can cause a stunted stature in the plant adult stage, and numerous defense response genes can be continuously activated. In this study, we performed a time-course transcriptome analysis of the early 4 weeks of leaf samples from wild plants and their fhy3 and far1 transcription factors. By comparative transcriptome analysis, we found that during the early 4 weeks of plant growth, plants primarily promoted morphogenesis by organizing their microtubules in the second week. In the third week, plants began to trigger large- scale defense responses to resist various external stresses. In the fourth week, increased photosynthetic efficiency promoted rapid biomass accumulation. Weighted gene coexpression network analysis of FHY3 and FAR1 revealed that the two light signaling transcription factors may be originally involved in the regulation of genes during embryonic development, and in the later growth stage, they might regulate gene expression of some defense-related genes to balance plant growth and immunity. Remarkably, our yeast two-hybrid and bimolecular fluorescence complementation experiments showed that FAR1 interacts with the immune signaling factor EDS1. Taken together, this study demonstrates the major biological processes occurring during the early 4 weeks of plant growth. The light signaling transcription factors, FHY3 and FAR1, may integrate light signals with immune signals to widely regulate plant growth by directly interacting with EDS1.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2020, 89, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differences in the expression of selected genes associated with cell stress response in a PTSD patient and in healthy individuals – a case study
    Autorzy:
    Rasmus, Anna
    Półrola, Paweł
    Góral-Półrola, Jolanta
    Żychowska, Małgorzata
    Mirski, Andrzej
    Knapik, Henryk
    Powiązania:
    https://bibliotekanauki.pl/articles/986669.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    cell stress
    ptsd patient
    expression of selected genes
    healthy individuals
    Opis:
    Objective. The aim of the study was determination of the level in expression genes associated with cell stress response in a patient with PTSD. Case study. A 57-year-old PTSD patient, A.P., was the subject of the study. Blood samples were taken from the ulnar vein to assess the relative expression of the tested genes in leukocytes via a quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results. A considerably decreased level in the expression HSF-1, NF-kB, Hif-1 and IL6 m-RNA was observed in the patient when compared to the control group. A low expression in HSPA1A, HSPB1, IL10 and CRP m-RNA compared with the control group was also noted. Conclusion. It is possible that the lower level of transcriptional factors: HSF-1, NF-kB and Hif-1 as well as IL6 m-RNA may be associated with the effect of the PTSD patient’s treatment.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2017, 24, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reference genes for gene expression studies on non-small cell lung cancer
    Autorzy:
    Gresner, Peter
    Gromadzinska, Jolanta
    Wasowicz, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1040589.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    non-small cell lung cancer
    reference genes
    data normalization
    real-time PCR
    gene expression
    Opis:
    Study Objective: The aim of this study was to test a panel of 6 reference genes in order to identify and validate the most suitable reference genes for expression studies in paired healthy and non-small cell lung cancer tissues. Method: Quantitative real-time PCR followed by the NormFinder- and geNorm-based analysis was employed. The study involved 21 non-small cell lung cancer patients. Results: The analysis of experimental data revealed HPRT1 as the most stable gene followed by RPLP0 and ESD. In contrast, GAPDH was found to be the least stable gene. HPRT1 together with ESD was revealed as the pair of genes introducing the least systematic error into data normalization. Validation by bootstrap random sampling technique and by normalizing exemplary gene expression data confirmed the results. Conclusion: Although HPRT1 and ESD may by recommended for data normalization in gene expression studies on non-small cell lung cancer, the suitability of selected reference genes must be unconditionally validated prior to each study.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 2; 307-316
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Przegląd badań nad regulacją ekspresji genów głównych odporności roślin na patogeny
    Studies review of gene expression regulation of the plant major resistance genes against pathogens
    Autorzy:
    Świątek, Mariusz
    Śliwka, Jadwiga
    Powiązania:
    https://bibliotekanauki.pl/articles/2198190.pdf
    Data publikacji:
    2011-12-29
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    ekspresja genów
    geny R
    odporność roślin
    patogeny roślin
    gene expression
    plant pathogens
    plant resistance
    R genes
    Opis:
    Rośliny, podobnie jak zwierzęta, wykształciły wielopoziomowe mechanizmy obronne, które nadają im odporność na szkodniki i patogeny. Pierwotna odpowiedź obronna nie zawsze jednak zapewnia wystarczający poziom odporności na patogeny, które dysponują szerokim wachlarzem mechanizmów przystosowawczych. Inny typ odporności jest warunkowany przez geny główne odporności — geny R. Kodowane przez nie białka, pełniące rolę receptorów, oddziałują z produktami genów awirulencji patogenów i uruchamiają szlak transdukcji sygnału, który prowadzi do reakcji nadwrażliwości, co zapobiega szerzeniu się infekcji. W związku z wysokimi zdolnościami adaptacyjnymi patogenów, poszukuje się nowych genów odporności w dzikich gatunkach roślin, które zapewniłyby roślinom uprawnym trwałą odporność. Mimo dużej liczby zidentyfikowanych i sklonowanych genów R, badaniami ekspresji objęto jak dotąd niewiele z nich. Geny R, pomimo podobieństw budowy, wykazują różny wzór ekspresji pod wpływem działania czynników biotycznych i abiotycznych. Większość genów R ulega konstytutywnej ekspresji, lecz obserwowane są także przypadki wzmacniania lub indukcji ekspresji w wyniku kontaktu z patogenem. Badania molekularne dotyczące ekspresji genów R mogą mieć w niedalekiej przyszłości zastosowanie aplikacyjne w selekcji roślin do hodowli odpornościowej, na co wskazują wyniki dotychczasowych doświadczeń. Celem przeglądu jest usystematyzowanie informacji uzyskanych z dotychczasowych badań nad ekspresją genów odporności roślin na patogeny.
    Plants, like animals, have developed multi-layered defense mechanisms that make them resistant to pests and pathogens. Innate basal defense response do not always provide a sufficient level of resistance to pathogens that use a wide range of adaptive mechanisms. Another type of resistance is that conferred by the major resistance genes — R genes. Proteins coded by those genes act as receptors that interact with the corresponding products of avirulence genes of the pathogens. They trigger then signal transduction pathway that leads to hypersensitive reaction, thus preventing the spreading of infection. Due to the high adaptability of pathogens, scientists are forced to search for new resistance genes in wild species of plants that would provide more durable resistance in crops. Despite the fact that the large number of R genes has been identified and cloned up to date, the expression surveys were performed only on a few of them. The R genes have structural similarities but often show different pattern of expression under the influence of biotic and abiotic factors. Most of the R genes are constitutively expressed, but there are also cases of enhancing or inducing the expression as a result of interaction with the pathogen. Molecular research including gene expression issue may have an application aspect in the near future in selection of plants for resistance breeding programs, as was demonstrated in the some experiments. The objective of this review is to pool the information obtained from previous studies on gene expression of plant resistance genes to pathogens.
    Źródło:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2011, 262; 89-101
    0373-7837
    2657-8913
    Pojawia się w:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
    Autorzy:
    Wolińska, L.
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Podlasz, P.
    Kolwicz, S.
    Piasecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363152.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    toksyczność ostra
    CYP1A
    cyp1b1
    geny vtg
    ekspresja mRNA
    stężenie nie wywołujące efektu
    WWA
    Real-Time qPCR
    acute toxicity
    cyp1a genes
    cyp1b1 genes
    vtg genes
    mRNA expression
    No Effect Concentration
    PAHs
    Opis:
    Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 26-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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