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    Tytuł:
    Calcium phosphate nanoparticles for gene transfer and silencing - a 2D and 3D cell culture model study
    Autorzy:
    Bialas, N.
    Sanchez, L. R.
    Sokolova, V.
    Epple, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/285631.pdf
    Data publikacji:
    2017
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    nanoparticles
    gene transfer
    silencing
    Źródło:
    Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 34
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The first evidence of a host-to-parasite mitochondrial gene transfer in Orobanchaceae
    Autorzy:
    Kwolek, Dagmara
    Denysenko-Bennett, Magdalena
    Góralski, Grzegorz
    Cygan, Magdalena
    Mizia, Patryk
    Piwowarczyk, Renata
    Szklarczyk, Marek
    Joachimiak, Andrzej J.
    Powiązania:
    https://bibliotekanauki.pl/articles/952048.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    orobanche
    hgt
    horizontal gene transfer
    atp6
    mitochondrion
    Opis:
    Several parasitic plants are known to have acquired mitochondrial genes via a horizontal transfer from their hosts. However, mitochondrial gene transfer in this direction has not yet been found in the parasite-rich family Orobanchaceae. Based on a phylogenetic analysis of the mitochondrial atp6 gene in selected species of Orobanche s.l., we provide evidence of a host-to-parasite transfer of this gene in O. coerulescens, which is a Eurasiatic species that parasitises Artemisia (Asteraceae). We did not find the original Orobanche atp6 gene in this species, which suggests that it has been replaced by a gene that was acquired from Asteraceae. In addition, our data suggest the occurrence of a second HGT event in the atp6 sequence – from Asteraceae to Phelipanche. Our results support the view that the transfer of genetic material from hosts to parasites influences the mitochondrial genome evolution in the latter.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Achievements in horizontal gene transfer studies in parasitic plants
    Autorzy:
    Góralski, G.
    Denysenko-Bennett, M.
    Burda, A.
    Staszecka-Moskal, N.
    Kwolek, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/2142106.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    evolution
    HGT
    horizontal gene transfer
    hosts
    parasitic plants
    Opis:
    Horizontal gene transfer (HGT) is a process that allows genetic material to flow between even distantly related organisms. It is primarily observed in bacteria and protists but also in different lineages of eucaryotes. The first HGT cases in plants were discovered at the beginning of the 21st century and have been intensively studied ever since. Researchers have placed particular emphasis on the plant kingdom, especially parasitic plants. This review presents the current state of knowledge about this phenomenon in plants, with a special focus on parasitic plants. Among the described factors facilitating HGT, close physical contact between organisms is believed to be one of the most important. It is noted especially in the case of parasitism and similar relationships. For that reason, reported occurrences of this phenomenon in holoparasites, hemiparasites, and mycoheterotrophic plants are compared. The mechanisms responsible for HGT in plants still remain unclear, however, the studies described here suggest that both DNA and RNA may play a role as a carrier in that process. Also, the transfer between genomes of different organelles in the cell, intracellular gene transfer (IGT), and its relationships with HGT are described. The occurrence of the HGT and IGT phenomena concerning different genomes: nuclear, mitochondrial, and plastid is discussed in the review. Finally, some future areas of research in the field are proposed.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2021, 63, 2; 17-28
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vivo gene transfer using cetylated polyethylenimine.
    Autorzy:
    Sochanik, Aleksander
    Cichoń, Tomasz
    Makselon, Monika
    Stróżyk, Małgorzata
    Smolarczyk, Ryszard
    Jazowiecka-Rakus, Joanna
    Szala, Stanisław
    Powiązania:
    https://bibliotekanauki.pl/articles/1041547.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    modified PEI
    gene transfer
    non-viral vectors
    cationic lipids
    Opis:
    This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be incorporated into liposomes in order to be suitable for gene transfer studies. Serum-induced plasmid DNA degradation assay demonstrated that CT-PEI-containing liposomal carriers could protect complexed DNA (probably via condensation). In vitro luciferase gene expression achieved using medium supplemented with 10% serum was comparable to that achieved in serum-reduced medium and was highest for CT-PEI/cholesterol liposomes, followed by CT-PEI/dioleoylphosphatidylcholine liposomes and PEI 600 Da (uncetylated) carrier. In vivo systemic transfer into mice was most efficient when liposome formulations contained CT-PEI and cholesterol. Higher luciferase expression was then observed in lungs than in liver. In conclusion: liposomes containing cetylated polyethylenimine and cholesterol are a suitable vehicle for investigating systemic plasmid DNA transfer into lungs.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 693-702
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Primary endosymbiosis: have cyanobacteria and Chlamydiae ever been roommates?
    Autorzy:
    Deschamps, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/56711.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    primary endosymbiosis
    endosymbiosis
    Cyanoprokaryota
    Chlamydia
    Archaeplastida
    gene transfer
    photosynthesis
    Opis:
    Eukaryotes acquired the ability to process photosynthesis by engulfing a cyanobacterium and transforming it into a genuine organelle called the plastid. This event, named primary endosymbiosis, occurred once more than a billion years ago, and allowed the emergence of the Archaeplastida, a monophyletic supergroup comprising the green algae and plants, the red algae and the glaucophytes. Of the other known cases of symbiosis between cyanobacteria and eukaryotes, none has achieved a comparable level of cell integration nor reached the same evolutionary and ecological success than primary endosymbiosis did. Reasons for this unique accomplishment are still unknown and difficult to comprehend. The exploration of plant genomes has revealed a considerable amount of genes closely related to homologs of Chlamydiae bacteria, and probably acquired by horizontal gene transfer. Several studies have proposed that these transferred genes, which are mostly involved in the functioning of the plastid, may have helped the settlement of primary endosymbiosis. Some of these studies propose that Chlamydiae and cyanobacterial symbionts coexisted in the eukaryotic host of the primary endosymbiosis, and that Chlamydiae provided solutions for the metabolic symbiosis between the cyanobacterium and the host, ensuring the success of primary endosymbiosis. In this review, I present a reevaluation of the contribution of Chlamydiae genes to the genome of Archaeplastida and discuss the strengths and weaknesses of this tripartite model for primary endosymbiosis.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Transient expression assay for optimization of direct gene transfer into cucumber meristem protoplasts by electroporation
    Autorzy:
    Burza, W
    Wochniak, P.
    Wroblewski, T.
    Malepszy, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2048135.pdf
    Data publikacji:
    1995
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    electroporation
    gene transfer
    protoplast
    transient expression
    cucumber
    Cucumis sativus
    Opis:
    The paper presents a new way of obtaining viable and very homogeneous cucumber protoplasts. Protoplasts from cells formed in the shoot tip meristem culture were isolated from suspension. Plasmid pBI121 was introduced using impulse electric field. Effectiveness of transformation process was determined by the transient expression of ß-glucuronidase (GUS) gene, controlled by promotor 35S. The activity of ß-glucuronidase enzyme as a product of GUS reporter gene was estimated by fluorimetric method (JEFFERSON 1987). Parameters of electroporation process were optimized. The transient expression of GUS gene was measured 24 h after electroporation. The highest effectiveness of transformation process was achieved using three electric impulses at the initial voltage of 250-350 V at 10-sec. intervals as a result of discharging a 140 µF capacitor and 50-70 µg × cm⁻³ plasmid DNA in the presence of 50 µg × cm⁻³ carrier DNA. The system presented is an effective method of exogenic DNA transfer, which is indicated by a high transient expression of the reporter gene. In comparison to Agrobacterium tumefaciens and A. rhizogenes, this alternative method of gene transfer can be used for obtaining transgenic cucumber plants.
    Źródło:
    Journal of Applied Genetics; 1995, 36, 1; 1-10
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Involvement of plastid, mitochondrial and nuclear genomes in plant-to-plant horizontal gene transfer
    Autorzy:
    Sanchez-Puerta, M.V.
    Powiązania:
    https://bibliotekanauki.pl/articles/57778.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    angiosperm
    plastid
    mitochondrial genome
    nuclear genome
    genome
    horizontal gene transfer
    gene conversion
    parasite
    Opis:
    This review focuses on plant-to-plant horizontal gene transfer (HGT) involving the three DNA-containing cellular compartments. It highlights the great incidence of HGT in the mitochondrial genome (mtDNA) of angiosperms, the increasing number of examples in plant nuclear genomes, and the lack of any convincing evidence for HGT in the well-studied plastid genome of land plants. Most of the foreign mitochondrial genes are non-functional, generally found as pseudogenes in the recipient plant mtDNA that maintains its functional native genes. The few exceptions involve chimeric HGT, in which foreign and native copies recombine leading to a functional and single copy of the gene. Maintenance of foreign genes in plant mitochondria is probably the result of genetic drift, but a possible evolutionary advantage may be conferred through the generation of genetic diversity by gene conversion between native and foreign copies. Conversely, a few cases of nuclear HGT in plants involve functional transfers of novel genes that resulted in adaptive evolution. Direct cell-to-cell contact between plants (e.g. host-parasite relationships or natural grafting) facilitate the exchange of genetic material, in which HGT has been reported for both nuclear and mitochondrial genomes, and in the form of genomic DNA, instead of RNA. A thorough review of the literature indicates that HGT in mitochondrial and nuclear genomes of angiosperms is much more frequent than previously expected and that the evolutionary impact and mechanisms underlying plant-to-plant HGT remain to be uncovered.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Plastid origin: who, when and why?
    Autorzy:
    Ku, Chuan
    Roettger, M.
    Zimorski, V.
    Nelsen-Sathi, S.
    Sousa, F.L.
    Martin, W.F.
    Powiązania:
    https://bibliotekanauki.pl/articles/59262.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    plastid
    Cyanoprokaryota
    endosymbiosis
    evolution
    gene transfer
    genomics
    organelle
    photosynthesis
    phylogenesis
    Opis:
    The origin of plastids is best explained by endosymbiotic theory, which dates back to the early 1900s. Three lines of evidence based on protein import machineries and molecular phylogenies of eukaryote (host) and cyanobacterial (endosymbiont) genes point to a single origin of primary plastids, a unique and important event that successfully transferred two photosystems and oxygenic photosynthesis from prokaryotes to eukaryotes. The nature of the cyanobacterial lineage from which plastids originated has been a topic of investigation. Recent studies have focused on the branching position of the plastid lineage in the phylogeny based on cyanobacterial core genes, that is, genes shared by all cyanobacteria and plastids. These studies have delivered conflicting results, however. In addition, the core genes represent only a very small portion of cyanobacterial genomes and may not be a good proxy for the rest of the ancestral plastid genome. Information in plant nuclear genomes, where most genes that entered the eukaryotic lineage through acquisition from the plastid ancestor reside, suggests that heterocyst-forming cyanobacteria in Stanier’s sections IV and V are most similar to the plastid ancestor in terms of gene complement and sequence conservation, which is in agreement with models suggesting an important role of nitrogen fixation in symbioses involving cyanobacteria. Plastid origin is an ancient event that involved a prokaryotic symbiont and a eukaryotic host, organisms with different histories and genome evolutionary processes. The different modes of genome evolution in prokaryotes and eukaryotes bear upon our interpretations of plastid phylogeny.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The evolution of land plants: a perspective from horizontal gene transfer
    Autorzy:
    Wang, Qia
    Sun, H.
    Huang, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/56538.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    land plant
    evolution
    horizontal gene transfer
    mitochondrial genome
    adaptation
    genetic information
    Opis:
    Recent studies suggest that horizontal gene transfer (HGT) played a significant role in the evolution of eukaryotic lineages. We here review the mechanisms of HGT in plants and the importance of HGT in land plant evolution. In particular, we discuss the role of HGT in plant colonization of land, phototropic response, C4 photosynthesis, and mitochondrial genome evolution.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2014, 83, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nonviral transfection of human umbilical cord blood dendritic cells is feasible, but the yield of dendritic cells with transgene expression limits the application of this method in cancer immunotherapy
    Autorzy:
    Markowicz, Sergiusz
    Niedzielska, Joanna
    Kruszewski, Marcin
    Ołdak, Tomasz
    Gajkowska, Agnieszka
    Machaj, Eugeniusz
    Skurzak, Henryk
    Pojda, Zygmunt
    Powiązania:
    https://bibliotekanauki.pl/articles/1041291.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CD34+ cells
    umbilical cord blood
    green fluorescent protein
    electroporation
    gene transfer
    dendritic cells
    Opis:
    Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein antigens for immunotherapy purposes. Cord blood mononuclear cells were cultured in serum-free medium in the presence of granulocyte-monocyte colony stimulating factor (GM-CSF), stem cell factor (SCF) and Flt-3 ligand (FL), to generate DC from their precursors, and thereafter transfected by electroporation. Maturation of DC was induced by stimulation with GM-CSF, SCF, FL and phorbol myristate acetate (PMA). Transfected DC strongly expressed EGFP, but transfection efficiency of DC, defined as HLA-DR+ cells lacking lineage-specific markers, did not exceed 2.5%. Expression of the reporter gene was also demonstrated in the DC generated from transfected, purified CD34+ cord blood cells, by stimulation with GM-CSF, SCF, FL, and tumor necrosis factor α (TNF-α). Transfection of CD34+ cells was very efficient, but proliferation of the transfected cells was much reduced as compared to the untransfected cells. Therefore, the yield of transgene-expressing DC was relatively low. In conclusion, nonviral transfection of cord blood DC proved feasible, but considering the requirements for immunotherapy in cancer patients, transfection of differentiated DC or generation of DC from transfected hematopoietic stem cells provide only a limited number of DC expressing the transgene.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 203-212
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nowe spojrzenie na bakteryjne patogeny odzwierzęce stanowiące zagrożenie dla człowieka
    New insight into bacterial zoonotic pathogens posing health hazards to humans
    Autorzy:
    Ciszewski, Marcin
    Czekaj, Tomasz
    Szewczyk, Eligia M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2166193.pdf
    Data publikacji:
    2015-02-18
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    epidemiologia
    bakteryjne infekcje odzwierzęce
    ewolucja patogenów
    przełamywanie barier międzygatunkowych
    różnorodność genetyczna
    poziomy transfer genów
    epidemiology
    bacterial zoonotic infections
    pathogen evolution
    crossing interspecies barriers
    genetic variation
    horizontal gene transfer
    Opis:
    W artykule omówiono zmiany, jakim podlegają patogeny zwierzęce, które na drodze ewolucji stały się chorobotwórcze dla ludzi. Ryzyko zakażenia dotyczy szczególnie osób mających bezpośredni kontakt ze zwierzętami w ramach obowiązków zawodowych – weterynarzy, hodowców, osób pracujących w zakładach zajmujących się ubojem lub przetwórstwem surowców pochodzenia zwierzęcego. W artykule przedstawiono drobnoustroje wskazywane w raportach epidemiologicznych Europejskiego Centrum Zapobiegania i Kontroli Chorób (European Centre for Disease Prevention and Control, ECDC), które od 19 lat gromadzi i publikuje w postaci raportów dane na temat zoonoz ze wszystkich krajów członkowskich Unii Europejskiej. Obecnie ECDC monitoruje występowanie chorób wywoływanych przez 11, uznanych za najważniejsze, czynników etiologicznych zoonoz, z których 7 stanowią patogeny bakteryjne: Salmonella spp., Campylobacter spp., Listeria monocytogenes, Mycobacterium bovis, Brucella spp., Coxiella burnetti oraz Verotoxin-producing E. coli (VTEC) / Shiga-like toxin-producing E. coli (STEC). Za szczególnie ważne uważa się te, które mogą rozprzestrzeniać się za pośrednictwem żywności. W artykule uwzględniono także wybrane nowe zagrożenia, niesione przez gatunki jeszcze niemonitorowane – paciorkowce Streptococcus iniae, S. suis i S. dysgalactiae oraz gronkowce Staphylococcus intermedius i S. pseudintermedius. Drobnoustroje te w ciągu ostatnich lat przełamały barierę międzygatunkową. Przyczyny tego zjawiska nie są znane, ale wiąże się je ze zmiennością drobnoustrojów oraz ich adaptacją do życia w zmieniającym się środowisku, co wynika z łatwości rearanżacji DNA i poziomego transferu genów. Skalę problemu nowych infekcji odzwierzęcych odzwierciedla istotny wzrost liczby publikacji w tej dziedzinie w ostatnich latach. Med. Pr. 2014;65(6):819–829
    This article presents the problem of evolutionary changes of zoonotic pathogens responsible for human diseases. Everyone is exposed to the risk of zoonotic infection, particularly employees having direct contact with animals, i.e. veterinarians, breeders, butchers and workers of animal products’ processing industry. The article focuses on pathogens monitored by the European Centre for Disease Prevention and Control (ECDC), which has been collecting statistical data on zoonoses from all European Union countries for 19 years and publishing collected data in annual epidemiological reports. Currently, the most important 11 pathogens responsible for causing human zoonotic diseases are being monitored, of which seven are bacteria: Salmonella spp., Campylobacter spp., Listeria monocytogenes, Mycobacterium bovis, Brucella spp., Coxiella burnetti and Verotoxin- producing E. coli (VTEC) / Shiga-like toxin producing E. coli (STEC). As particularly important are considered foodborne pathogens. The article also includes new emerging zoonotic bacteria, which are not currently monitored by ECDC but might pose a serious epidemiological problem in a foreseeable future: Streptococcus iniae, S. suis, S. dysgalactiae and staphylococci: Staphylococcus intermedius, S. pseudintermedius. Those species have just crossed the animal-human interspecies barrier. The exact mechanism of this phenomenon remains unknown, it is connected, however, with genetic variability, capability to survive in changing environment. These abilities derive from DNA rearrangement and horizontal gene transfer between bacterial cells. Substantial increase in the number of scientific publications on this subject, observed over the last few years, illustrates the importance of the problem. Med Pr 2014;65(6):819–829
    Źródło:
    Medycyna Pracy; 2014, 65, 6; 819-829
    0465-5893
    2353-1339
    Pojawia się w:
    Medycyna Pracy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
    Autorzy:
    Humphreys, M W
    Thomas, H M
    King, I P
    Morgan, W G
    Meredith, M R
    Harper, J A
    Humphreys, M O
    Bettany, A J E
    Dalton, S J
    James, A R
    Ougham, H J
    Thomas, H
    Powiązania:
    https://bibliotekanauki.pl/articles/2046675.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    RNA
    in situ
    tissue culture
    gene transfer
    chromosome segment
    anther culture
    androgenesis
    plant breeding
    introgression mapping
    fluorescence
    hybridization
    Lolium-Festuca complex
    drought resistance
    gene isolation
    tetraploid hybrid
    DNA
    meiosis
    grass
    chromosome behaviour
    Opis:
    Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 3; 273-284
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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