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Wyszukujesz frazę "gene regulation" wg kryterium: Temat

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    Wyświetlanie 1-13 z 13
    Tytuł:
    Genetic regulation of alpha-amylase synthesis in rye [Secale cereale L.] grain
    Autorzy:
    Masojc, P
    Stojalowski, S
    Lapinski, M
    Miazga, D
    Powiązania:
    https://bibliotekanauki.pl/articles/2047279.pdf
    Data publikacji:
    1996
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    enzyme synthesis
    activity
    chromosome
    rye
    Secale cereale
    crossing
    alpha-amylase
    sprouting line
    duplicate gene
    addition line
    genetic regulation
    Opis:
    Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal addition lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain. The long arm of chromosome 6R was found to be responsible for increased enzyme synthesis during late stages of triticale grain maturation. Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects. Low enzyme activity showed complete dominance over high level of its synthesis. Segregation ratios, observed in F₂ and BC₁ crosses, indicated that recessive alleles at two independent duplicative loci underlie intensive alpha-amylase production.
    Źródło:
    Journal of Applied Genetics; 1996, 37, 2; 141-152
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. V. Different mechanisms of regulation regarding the open reading frames
    Autorzy:
    Borowicz, B P
    Powiązania:
    https://bibliotekanauki.pl/articles/64980.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    regulation mechanism
    gene expression
    I-18 C gene
    Chironomus tentans
    polymerase chain reaction
    DNA fragment
    Opis:
    Different mechanisms of regulation regarding the Open Reading Frames (ORFs) have been discussed to have a broader perspective that is necessary to evaluate the role of the ORFs of the I-18 C gene. This consideration includes the ORF II of the I-18 C gene which is the object of the presented research.
    Omówiono różnorodne mechanizmy regulacji dotyczące Otwartych Ram Odczytu, w celu wytworzenia szerszego spojrzenia na to zagadnienie, które jest potrzebne do oceny roli poszczególnych Otwartych Ram Odczytu (ORF) genu I-18 C. Omówienie to dotyczy również ORF II genu I-18 C, co było przedmiotem zaprezentowanych badań.
    Źródło:
    Journal of Plant Protection Research; 1997, 37, 1-2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.
    Autorzy:
    Czyż, Małgorzata
    Stasiak, Marta
    Boncela, Joanna
    Cierniewski, Czesław
    Powiązania:
    https://bibliotekanauki.pl/articles/1043802.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    αvβreceptors
    transcription regulation
    integrins
    gene expression
    Opis:
    Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αV promoter region and are directly involved in the regulation of transcriptional activity of the αV gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αV expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the αV gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced αV mRNA and αV protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αV. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the αV gene promoter and negatively regulates αV gene expression.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 1; 19-28
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metabolic enzymes that bind RNA: yet another level of cellular regulatory network?
    Autorzy:
    Cieśla, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041266.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    enzymes
    gene expression
    mRNA binding
    translation regulation
    Opis:
    Several enzymes that were originally characterized to have one defined function in intermediatory metabolism are now shown to participate in a number of other cellular processes. Multifunctional proteins may be crucial for building of the highly complex networks that maintain the function and structure in the eukaryotic cell possessing a relatively low number of protein-encoding genes. One facet of this phenomenon, on which I will focus in this review, is the interaction of metabolic enzymes with RNA. The list of such enzymes known to be associated with RNA is constantly expanding, but the most intriguing question remains unanswered: are the metabolic enzyme-RNA interactions relevant in the regulation of cell metabolism? It has been proposed that metabolic RNA-binding enzymes participate in general regulatory circuits linking a metabolic function to a regulatory mechanism, similar to the situation of the metabolic enzyme aconitase, which also functions as iron-responsive RNA-binding regulatory element. However, some authors have cautioned that some of such enzymes may merely represent "molecular fossils" of the transition from an RNA to a protein world and that the RNA-binding properties may not have a functional significance. Here I will describe enzymes that have been shown to interact with RNA (in several cases a newly discovered RNA-binding protein has been identified as a well-known metabolic enzyme) and particularly point out those whose ability to interact with RNA seems to have a proven physiological significance. I will also try to depict the molecular switch between an enzyme's metabolic and regulatory functions in cases where such a mechanism has been elucidated. For most of these enzymes relations between their enzymatic functions and RNA metabolism are unclear or seem not to exist. All these enzymes are ancient, as judged by their wide distribution, and participate in fundamental biochemical pathways.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 11-32
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A common cis-element in promoters of protein synthesis and cell cycle genes
    Autorzy:
    Wyrwicz, Lucjan
    Gaj, Paweł
    Hoffmann, Marcin
    Rychlewski, Leszek
    Ostrowski, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1041116.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    gel shift assay
    regulation of transcription
    comparative genomics
    gene expression
    promoter
    bioinformatics
    Opis:
    Gene promoters contain several classes of functional sequence elements (cis elements) recognized by protein agents, e.g. transcription factors and essential components of the transcription machinery. Here we describe a common DNA regulatory element (tandem TCTCGCGAGA motif) of human TATA-less promoters. A combination of bioinformatic and experimental methodology suggests that the element can be critical for expression of genes involved in enhanced protein synthesis and the G1/S transition in the cell cycle. The motif was identified in a substantial fraction of promoters of cell cycle genes, like cyclins (CCNC, CCNG1), as well as transcription regulators (TAF7, TAF13, KLF7, NCOA2), chromatin structure modulators (HDAC2, TAF6L), translation initiation factors (EIF5, EIF2S1, EIF4G2, EIF3S8, EIF4) and previously reported 18 ribosomal protein genes. Since the motif can define a subset of promoters with a distinct mechanism of activation involved in regulation of expression of about 5% of human genes, further investigation of this regulatory element is an emerging task.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 1; 89-98
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    STAT activation and differential complex formation dictate selectivity of interferon responses
    Autorzy:
    Wesoly, Joanna
    Szweykowska-Kulinska, Zofia
    Bluyssen, Hans
    Powiązania:
    https://bibliotekanauki.pl/articles/1041103.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    transcription factor complexes
    gene regulation
    IRFs
    JAK/STAT pathway
    IFNs
    Opis:
    Interferons (IFNs) induce gene expression by phosphorylating latent transcription factors belonging to the signal transducer and activator of transcription (STAT) family, mediated by janus kinases (Jaks). STAT dimers directly activate genes containing the IFNγ activation site (GAS) DNA element, with different STAT proteins displaying slightly different intrinsic DNA binding specificities. The combinatorial association of STATs with the additional DNA binding adaptor protein interferon regulatory factor (IRF)9 expands the range of enhancer elements that can be targeted by the JAK-STAT pathway to interferon-stimulated response element (ISRE) and IRF response element (IRE). Based on the amino-acid sequence similarity within the IRF family and functional overlap with the STAT family, in this paper we hypothesize that other IRF members could serve as adapter proteins for the STATs during IFN responses to redirect them to subsets of ISRE, GAS and/or IRE-containing IFN-stimulated genes (ISGs). In addition, the fact that STAT2 homodimers are not capable of binding consensus GAS sites leaves the possibility for a novel type of DNA-binding site bound by STAT2 homodimers and potentially other STAT complexes.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 1; 27-38
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene
    Autorzy:
    Widłak, Wiesława
    Vydra, Natalia
    Dudaladava, Volha
    Ścieglińska, Dorota
    Winiarski, Bolesław
    Krawczyk, Zdzisław
    Powiązania:
    https://bibliotekanauki.pl/articles/1041120.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Sp1
    GC-box
    regulation of gene expression
    spermatogenesis
    heat shock protein
    Opis:
    The Hsp70.2/Hst70 gene, which belongs to the 70 kDa heat-shock protein (HSP) family, is expressed specifically in primary spermatocytes and spermatids. The regulatory elements required for a high level of testis-specific expression of the gene are placed between the two major transcription start sites T1 and T2 (approximately 350 and 115 bp upstream of the starting ATG codon). Here we have shown that sequences proximal to the exon1/intron splicing site in the 5' untranslated region of the Hsp70.2/Hst70 gene, which include a highly conserved element called box B, are required for efficient expression of the chloramphenicol acetyltransferase reporter gene in testes of transgenic mice. However, in spite of the drastically reduced overall activity, the stage-specific expression pattern of the transgene was preserved after removal of these sequences. We have also shown that GC-box located downstream of the box B (approximately 210 bp upstream of the starting ATG codon) is indispensable for efficient expression of the Hsp70.2/Hst70 gene promoter in spermatogenic cells. The GC-box specifically binds proteins present in nuclear extracts from testes (putatively Sp1-like factors). A change in the pattern of such GC-box-interacting factors corresponds to activation of the Hsp70.2/Hst70 gene, confirming the importance of this regulatory element.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 1; 107-112
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Position weight matrix model as a tool for the study of regulatory elements distribution across the DNA sequence
    Autorzy:
    Jaksik, R.
    Rzeszowska-Wolny, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/229738.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    transcription factors
    TFBS
    regulation of gene expression
    regulatory sequence elements
    DNA
    position weight matrix
    PWM
    Opis:
    Ab initio methods of DNA regulatory sequence region prediction known as transcription factor binding sites (TFBS) are a very big challenge to modern bioinformatics. Although the currently available methods are not perfect they are fairly reliable and can be used to search for new potential protein-DNA interaction sites. The biggest problem of ab initio approaches is the very high false positive rate of predicted sites which results mainly from the fact that TFBS are very short and highly degenerate. Because of that they can occur by chance every few hundred bases making the task of computational prediction extremely difficult if one aims to reduce the high false positive rate keeping highest possible sensitivity to predict biologically meaningful sequence regions. In this work we present a new application that can be used to predict TFBS regions in very large datasets based on position weight matrix models (PWM’s) using one of the most popular prediction methods. The presented application was used to predict the concentration of TFBS in a set of nearly 2.2 thousand unique sequences of human gene promoter regions. The study revealed that the concentration of TFBS further than 1kbp from the transcription initiation site is constant but it decreases rapidly while getting closer to the transcription initiation site. The decreasing TFBS concentration in the vicinity of genes might result from evolutionary selection which keeps only sites responsible for interactions with proteins being part of a specific regulatory mechanism leading to cells survival.
    Źródło:
    Archives of Control Sciences; 2010, 20, 4; 491-501
    1230-2384
    Pojawia się w:
    Archives of Control Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A membrane bound NAC transcription factor is a regulator of mitochondrial retrograde regulation of the oxidative stress
    Autorzy:
    Van Breusegem, F.
    De Clercq, I.
    Vermeirssen, V.
    Van Aken, O.
    Wheelan, J.
    Vandepoele, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/80443.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    NAC transcription factor
    mitochondrial retrograde regulation
    reactive oxygen species
    protein signalling
    gene expression
    oxidative stress
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Apoplastic ROS sensing and signalling
    Autorzy:
    Kangasjarvi, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/80047.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    reactive oxygen species
    signalling
    plant cell
    stress adaptation
    acclimation
    cysteine-rich protein
    extracellular protein
    gene regulation
    marker gene
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cis-, trans-, transcriptional regulation of the Arabidopsis thaliana gene AtTrxo1 and its response upon germination and to salt
    Autorzy:
    Ortiz-Espin, A.
    Iglesias-Fernandez, R.
    Martinez-Alcala, I.
    Calderon, A.
    Camejo, D.
    Sevilla, F.
    Carbonero, P.
    Jimenez, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/80149.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    transcription regulation
    Arabidopsis thaliana
    thioredoxin
    germination
    salt stress
    abiotic stress
    gene regulation
    cis-element
    T-DNA
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comprehensive studies on biogenesis and function of tRNA-derived small noncoding RNAs in Arabidopsis thaliana
    Autorzy:
    Plewka, P.
    Kalak, M.
    Raczynska, K.D.
    Szymanski, M.
    Szweykowska-Kulinska, Z.
    Jarmolowski, A.
    Karlowski, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/80052.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    biogenesis
    molecular mechanism
    small RNA
    gene expression
    plant growth regulation
    environmental stress
    microRNA
    siRNA
    non-coding RNA
    Arabidopsis thaliana
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    AmiRNA Designer - new method of artificial miRNA design
    Autorzy:
    Mickiewicz, Agnieszka
    Rybarczyk, Agnieszka
    Sarzynska, Joanna
    Figlerowicz, Marek
    Blazewicz, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1038843.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    artificial miRNA
    RNAi
    gene regulation
    sequence specific
    thermodynamic profiles
    Opis:
    MicroRNAs (miRNAs) are small non-coding RNAs that have been found in most of the eukaryotic organisms. They are involved in the regulation of gene expression at the post-transcriptional level in a sequence specific manner. MiRNAs are produced from their precursors by Dicer-dependent small RNA biogenesis pathway. Involvement of miRNAs in a wide range of biological processes makes them excellent candidates for studying gene function or for therapeutic applications. For this purpose, different RNA-based gene silencing techniques have been developed. Artificially transformed miRNAs (amiRNAs) targeting one or several genes of interest represent one of such techniques being a potential tool in functional genomics. Here, we present a new approach to amiRNA*design, implemented as AmiRNA Designer software. Our method is based on the thermodynamic analysis of the native miRNA/miRNA* and miRNA/target duplexes. In contrast to the available automated tools, our program allows the user to perform analysis of natural miRNAs for the organism of interest and to create customized constraints for the design stage. It also provides filtering of the amiRNA candidates for the potential off-targets. AmiRNA Designer is freely available at http://www.cs.put.poznan.pl/arybarczyk/AmiRNA/.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 1; 71-77
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

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