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    Wyświetlanie 1-4 z 4
    Tytuł:
    Inhibition of poly(ADP-ribose) polymerase activity affects its subcellular localization and DNA strand break rejoining
    Autorzy:
    Ryabokon, Nadezhda
    Cieślar-Pobuda, Artur
    Rzeszowska-Wolny, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1040571.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    DNA strand break rejoining
    efficiency of PARP inhibition
    PARP foci
    poly(ADP-ribose) polymerase (PARP)
    PARP inhibitors
    Opis:
    Poly(ADP-ribose) polymerase (PARP) plays a crucial role in DNA repair. Modulation of its activity by stimulation or inhibition is considered as a potentially important strategy in clinical practice, especially to sensitize tumor cells to chemo- and radiotherapy through inhibition of DNA repair. Here we studied the effect of the three PARP inhibitors, 5-iodo-6-amino-benzopyrone (INH2BP), 1,5-isoquinolinediol (1,5-dihydroxyisoquinolinediol (1,5-IQD) and 8-hydroxy-2-methylquinazolin-4-[3H]one (NU1025), and for two of them the efficiency in slowing the rejoining of DNA strand breaks induced by H2O2 was compared. Inhibition of PARP changed its intranuclear localization markedly; cells exposed to the inhibitor NU1025 showed a significant tendency to accumulate PARP in large foci, whereas in untreated cells its distribution was more uniform. The speed and efficiency of rejoining of H2O2-induced DNA strand breaks were lower in cells incubated with a PARP inhibitor, and the kinetics of rejoining were modulated in a different manner by each inhibitor. At a concentration of 100 µM the efficiency of the inhibitors could be ranked in the order NU1025 > IQD > INH2BP. The two first compounds were able to decrease the overall PARP activity below the level detected in control cells, while INH2BP showed up to 40% PARP activity after exposure to H2O2.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 2; 243-248
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    DNA damage in subpopulations of human lymphocytes irradiated with doses in the range of 0-1 Gy of X-radiation
    Autorzy:
    Wojewódzka, M.
    Machaj, E. K.
    Goździk, A.
    Iwaneńko, T.
    Ołdak, T.
    Kruszewski, M.
    Pojda, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/147760.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    alkaline comet assay
    biodosimetry
    micronucleus test
    histone gammaH2AX foci immunofluorescence assay
    T lymphocytes
    B lymphocytes
    ionizing radiation
    DNA damage
    Opis:
    We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0-1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gammaH2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gammaH2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively.
    Źródło:
    Nukleonika; 2008, 53, 4; 145-149
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Concerted control of DNA double strand break repair and cell cycle progression in X-irradiated mammalian cells
    Autorzy:
    Sochanowicz, B.
    Szumiel, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/148697.pdf
    Data publikacji:
    2005
    Wydawca:
    Instytut Chemii i Techniki Jądrowej
    Tematy:
    ATM kinase
    DNA double strand break repair
    RAD50
    cell cycle arrest
    repair foci
    BRCT domain
    Opis:
    Upon examination of cell cycle regulation in a damaged cell, relations were discovered of the cell cycle control mechanisms with a complicated web of signalling pathways, eventually called the genome surveillance system. After infliction of DNA double strand breaks (DSB), the signalling is initiated by sensor proteins and transducer protein kinase ATM. This kinase phosphorylates downstream effector proteins, such as checkpoint kinases CHK1 and CHK2, which initiate the pathways leading to cell cycle arrest. In contrast with the older model of linear transmission of signals in a certain sequence, it is now accepted that the damage signalling system is branched and contains feedback loops. DSB's presence is signalled by sensor proteins (MRE11-RAD50-nibrin complex, MRN) to ATM and the signal is amplified through adaptor proteins, MDC1/NFBD1 or 53BP1 (Tp53 binding protein). MRN contains a forkheadassociated (FHA) domain and BRCA1 carboxyl-terminal (BRCT) domain. The combination of the FHA/BRCT domains has a crucial role for the binding of nibrin to the H2AX histone, assembling the components of repair foci. These domains also are important for interaction of other proteins localised in the foci. For example, MDC1/NFBD1 contains a FHA domain and two BRCT domains which are involved in protein interactions. The signal generated at DSBs is amplified and transduced to recruit components of DNA repair systems. In a concerted way with the sequential recruitment of components of repair foci, activation of transcription of genes takes place, that is necessary for blocking progression through the cell cycle, for DNA repair or apoptosis.
    Źródło:
    Nukleonika; 2005, 50, 4; 129-138
    0029-5922
    1508-5791
    Pojawia się w:
    Nukleonika
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of post-blasting source mechanisms of mininginduced seismic events in Rudna copper mine, Poland
    Autorzy:
    Caputa, A.
    Talaga, A.
    Rudziński, Ł.
    Powiązania:
    https://bibliotekanauki.pl/articles/100792.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Śląski. Wydział Nauk o Ziemi
    Tematy:
    source mechanism
    blasting
    foci
    analiza
    mechanizm
    górnictwo
    zdarzenia sejsmiczne
    kopalnia miedzi Rudna
    Polska
    Opis:
    The exploitation of georesources by underground mining can be responsible for seismic activity in areas considered aseismic. Since strong seismic events are connected with rockburst hazard, it is a continuous requirement to reduce seismic risk. One of the most effective methods to do so is blasting in potentially hazardous mining panels. In this way, small to moderate tremors are provoked and stress accumulation is substantially reduced. In this paper we present an analysis of post-blasting events using Full Moment Tensor (MT) inversion at the Rudna mine, Poland, underground seismic network. In addition, we describe the problems we faced when analyzing seismic signals. Our studies show that focal mechanisms for events that occurred after blasts exhibit common features in the MT solution. The strong isotropic and small Double Couple (DC) component of the MT, indicate that these events were provoked by detonations. On the other hand, post-blasting MT is considerably different than the MT obtained for strong mining events. We believe that seismological analysis of provoked and unprovoked events can be a very useful tool in confirming the effectiveness of blasting in seismic hazard reduction in mining areas.
    Źródło:
    Contemporary Trends in Geoscience; 2015, 4, 1; 26-38
    2299-8179
    Pojawia się w:
    Contemporary Trends in Geoscience
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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