Temat: flow cytometry - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Flow cytometric estimation of ploidy in anisoploid sugar-beet populations
Autorzy:: Sliwinska, E
Steen, P.
Powiązania:: https://bibliotekanauki.pl/articles/2048141.pdf
Data publikacji:: 1995
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: production
Beta vulgaris
poliploid cultivar
ploidy determination
nuclear DNA
flow cytometry
DNA
seed
sugar-beet
Opis:: Determination of ploidy level is a common practice in sugar-beet breeding and seed production. Lately many laboratories have replaced microscopic chromosome counting by flow cytometric estimation of the nuclear DNA content. The possibility of direct estimation of cytological composition in anisoploid populations instead of individual plant analyses was investigated. Diploid, triploid and tetraploid sugar-beet lines as well as their mixtures were tested. The results obtained show that the suggested method is fast, simple and, after a proper transformation of the computer values, could be applied in breeding and seed production of polyploid crops. Precise estimation of contamination by small admixture of seeds or plants of undesirable ploidy, however, requires individual plant rather than population analyses.
Źródło:: Journal of Applied Genetics; 1995, 36, 2; 111-118
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Application of flow cytometry for the study of plant genomes
Autorzy:: Dolezel, J
Powiązania:: https://bibliotekanauki.pl/articles/2046673.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ploidy
DNA content
chromosome
blood cell
flow karyotype
optical detector system
flow cytogenetics
flow cytometry
genome
nucleus
plant
Opis:: During the last fifteen years, flow cytometry and sorting greatly contributed to the improvement of our knowledge of plant genome structure and function. This paper reviews the applications of flow cytometry for the analysis of isolated nuclei and chromosomes. Because of its speed, precision and convenience, this method of analysis of the nuclear DNA content finds an enormous number of applications which cover basic research, breeding and production. The results obtained with chromosome analysis and sorting indicate that the technique might greatly simplify the analysis of plant genomes at the molecular level.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 285-302
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Estimation of the share of components of different ploidy in anisoploid sugar-beet [Beta vulgaris L.] populations by flow cytometry
Autorzy:: Sliwinska, E
Jansen, R
Powiązania:: https://bibliotekanauki.pl/articles/2046829.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Beta vulgaris
ploidy determination
breeding
flow cytometry
seed production
sugar-beet
Opis:: Flow cytometry is commonly used in sugar-beet breeding and seed production for ploidy estimation. By means of this method individual plants as well as plant populations can be analysed. An interpretation of histograms obtained from anisoploid populations, however, causes some problems to the breeder. Bulks of diploid, triploid and tetraploid sugar-beet plants in different proportion and ploidy combinations (2x + 3x, 2x + 4x, and 3x + 4x) were tested in order to determine the relation between the actual share of the components of different ploidy levels in tested populations and that calculated on the basis of flow cytometry. In this way agraph for estimation of the share of different ploidy components was obtained. Repeatability of the results proves the usefulness of flow cytometric analysis of anisoploid plant populations in breeding and seed production of polyploid crops.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 151-160
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Cytostatic and cytotoxic effects of (E)-2'-deoxy-2'-(fluoromethylene)-cytidine on a solid tumor and a leukemia cell line.
Autorzy:: Grieb, Paweł
Koronkiewicz, Mirosława
Skierski, Janusz
Powiązania:: https://bibliotekanauki.pl/articles/1044427.pdf
Data publikacji:: 2000
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: cytotoxicity
flow cytometry
fluoromethylenedeoxycytidine
apoptosis
Opis:: (E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a human colorectal carcinoma line COLO-205. Despite dCK activity being by far the highest in cells of lymphoid origin, the effects of FMdC were detectable at the lowest drug concentration only in a solid tumor cell line, and at higher concentrations they were qualitatively similar in the two tumor lines (increased cell protein content, cell cycle block and apoptosis). Apparently, low dCK activity in solid tumor cells sufficiently activates FMdC to yield cytotoxic effects, while high dCK activity in leukemia cells does not increase its cytotoxicity.
Źródło:: Acta Biochimica Polonica; 2000, 47, 1; 165-171
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Gemini (dimeric) surfactant perturbation of the human erythrocyte.
Autorzy:: Dubnièková, Martina
Bobrowska-Hägerstrand, Malgorzata
Söderström, Thomas
Igliè, Aleš
Hägerstrand, Henry
Powiązania:: https://bibliotekanauki.pl/articles/1044306.pdf
Data publikacji:: 2000
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: TEM
flow cytometry
protection against hypotonic haemolysis
exovesiculation
human erythrocyte
detergent
cationic amphiphile
shape alteration
haemolysis
Opis:: We studied the ability of di-cationic gemini surfactantsdi (amphiphiles), i.e. 1,4-butanediammonium-N,N-dialkyl-N,N,N',N'-tetramethyl bromides (Di-Cm-di-QAS (s = 4), where m = 8,11,13,16 and s = the number of alkyl groups in the spacer) to induce shape alteration, vesiculation, haemolysis and phosphatidylserine exposure in human erythrocytes, and to protect erythrocytes against hypotonic haemolysis. At high sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles rapidly induced echinocytic (spiculated) shapes and a release of exovesicles, mainly in the form of tubes, from the cell surface. Following 60 min incubation erythrocytes were sphero-echinocytic and a few cells with invaginations/endovesicles were observed. No phosphatidylserine exposure was detected. The haemolytic potency increased with an increase of the alkyl chain length. At sublytic concentrations the Di-Cm-di-QAS (s = 4) amphiphiles protected erythrocytes against hypotonic haemolysis. It is suggested that the Di-Cm-di-QAS (s = 4) amphiphiles perturb the membrane in a similar way as single-chain cationic amphiphiles, but that they do not easily translocate to the inner membrane leaflet.
Źródło:: Acta Biochimica Polonica; 2000, 47, 3; 651-660
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Comparative studies of nuclear DNA content in benign and malignant thyroid lesions.
Autorzy:: Konarska, Liliana
Skierski, Janusz
Ellert, Aleksandra
Steinbrich, Justyna
Woźniak, Agnieszka
Kałczak, Mirosław
Powiązania:: https://bibliotekanauki.pl/articles/1044110.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: DNA content
thyroid
paraffin-embedded tissue
flow cytometry
ploidy
proliferative index
DNA index
Opis:: Currently available data suggest that DNA aneuploidy is associated with aggressive behavior of and unfavorable prognosis in several malignant human tumors as compared with diploid malignancies. However, the diagnostic and prognostic importance of flow cytometric DNA measurements in the case of thyroid neoplasms remains controversial. Therefore, the aim of our study was to evaluate utility of DNA index (DI) and proliferative index (PI) in distinguishing benign from malignant thyroid lesions taking into account the possible influence of intra-tumor heterogeneity and tissue preparation mode on DNA flow-cytometry measurements. A retrospective study was performed on 71 paraffin-embedded specimens from 57 patients with benign and malignant thyroid pathologies: 13 colloid goitres, 12 parenchymatous goitres, 19 adenomas and 13 carcinomas. In 14 of 57 cases two separate specimens taken from different areas of the same lesion were analysed and DNA parameters were compared. Additionally, flow cytometry DNA analysis was parallelly performed on 3 adjacent but differently processed tissue sections (fresh, formalin-fixed and paraffin-embedded) taken from each of 26 surgically excised thyroid lesions. DNA content was also analysed in both fresh and formalin-fixed twin specimens of normal pig thyroid glands (N = 6). We demonstrated that all tumors diagnosed as thyroid carcinomas were associated with abnormal nuclear DNA content although aneuploidy was not found specific to malignant thyroid tumors. Aneuploid samples of benign thyroid lesions exhibited higher proliferative activity, expressed as mean PI values, than diploid ones. In carcinomas the mean PI values were significantly higher than in benign lesions, independently whether they concerned aneuploid or diploid tissues. Considering intra-tumor heterogeneity, the flow cytometric DNA parameters can be assumed as reproducible despite differences in the mode of tissue fixation and preparation for analysis.
Źródło:: Acta Biochimica Polonica; 2001, 48, 3; 783-793
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Karyological characterization of sugar beet gynogenetic lines cultured in vitro
Autorzy:: Svirshchevskaya, A
Dolezel, J.
Powiązania:: https://bibliotekanauki.pl/articles/2041934.pdf
Data publikacji:: 2001
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ploidy
doubled haploid
chromosome doubling
flow cytometry
DNA
in vitro
gynogenetic line
sugar-beet
Źródło:: Journal of Applied Genetics; 2001, 42, 1; 21-32
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: The flavonoids, quercetin and isorhamnetin 3-O-acylglucosides diminish neutrophil oxidative metabolism and lipid peroxidation.
Autorzy:: Zielińska, Małgorzata
Kostrzewa, Artur
Ignatowicz, Ewa
Budzianowski, Jaromir
Powiązania:: https://bibliotekanauki.pl/articles/1044184.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: flow cytometry
respiratory burst
polymorphonuclear neutrophils
reactive oxygen species
lipid peroxidation
flavonoids
Opis:: Two natural flavonoids, quercetin and isorhamnetin 3-O-acylglucosides, were examined for their inhibitory influence on the in vitro production and release of reactive oxygen species in polymorphonuclear neutrophils (PMNs). The generation of superoxide radical, hydrogen peroxide and hypochlorous acid were measured by, respectively, cytochrome c reduction, dichlorofluorescin oxidation and taurine chlorination. Membrane lipid oxidation was studied by the thiobarbituric acid method in mouse spleen microsomes. The addition of flavonoids at the concentration range 1-100 μM inhibited PMNs oxidative metabolism and lipid peroxidation in a dose-dependent manner. The results suggest that these flavonoids suppress the oxidative burst of PMNs and protect membranes against lipid peroxidation.
Źródło:: Acta Biochimica Polonica; 2001, 48, 1; 183-189
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Partial characterization of human choriocarcinoma cell line JAR cells in regard to oxidative stress.
Autorzy:: Hallmann, Anna
Klimek, Jerzy
Masaoka, Makoto
Kamiński, Marcin
Kędzior, Jakub
Majczak, Anna
Niemczyk, Edyta
Woźniak, Michał
Trzonkowski, Piotr
Wakabayashi, Takashi
Powiązania:: https://bibliotekanauki.pl/articles/1041517.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: menadione
flow cytometry
JAR cells
placenta
H2O2
oxidative stress
Opis:: Characterization of free radical-induced cell injury processes of placenta cells is of vital importance for clinical medicine for the maintenance of intrauterine fetal life. The present study has analyzed cell injury processes in cells of the choriocarcinoma cell line JAR treated with menadione, an anticancer drug, and Hg2O2 in comparison to osteosarcoma 143B cells using electron microscopic and flow cytometric techniques. Flow cytometry on JAR cells exposed to 100 μM menadione and double-stained with Annexin V and propidium iodide (PI) detected apoptotic cells reaching the maximum after 4 h of incubation with a rapid decrease thereafter. Viable cells became decreased to 46% of the control after 2 h of incubation, reaching 5% after 4 h. Cells stainable with both Annexin V and PI began to increase distinctly after 2 h of incubation, reaching 55% after 4 h. Electron microscopy showed that cells stainable with both dyes specified above had condensed nuclei and swollen cytoplasm, suggesting that they were undergoing a switch of the cell death mode from apoptosis to necrosis. On the other hand, 90% of 143B cells remained intact after 4 h of menadione treatment although the intracellular levels of superoxide were always higher than those of JAR cells treated with the drug. In contrast, JAR cells were more resistant than 143B cells to H2O2-induced cytotoxicity. These results may suggest that cytotoxicity of menadione cannot be explained simply by oxygen free radicals generated from the drug. The resistance of JAR cells to oxygen free radical-induced cytotoxicity may be advantageous for intrauterine fetal life.
Źródło:: Acta Biochimica Polonica; 2004, 51, 4; 1023-1038
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Cytotoxic effects of cladribine and tezacitabine toward HL-60.
Autorzy:: Stachnik, Krzysztof
Grieb, Paweł
Skierski, Janusz
Powiązania:: https://bibliotekanauki.pl/articles/1041452.pdf
Data publikacji:: 2005
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: tezacitabine
cladribine
flow cytometry
nucleoside analogs
leukemia
HL-60 cells
Opis:: The aim of the study was to determine the relation between the cytotoxic and cytostatic effects of tezacitabine and cladribine on a HL-60 cell line and the time of exposure of cells to these drugs. Cell viability and induction of apoptosis were assessed using flow cytometry methods. Apoptosis was confirmed by direct microscopic observation. Growth inhibition was examined by cell counting. After 24 h incubation tezacitabine was equally or less toxic compared to cladribine. However, toxicity of tezacitabine strongly rose after 48 h incubation leading to massive cell death at doses much lower than those of cladribine. Assessment of the effect of increased exposure time on the clinical efficacy of tezacitabine is indicated.
Źródło:: Acta Biochimica Polonica; 2005, 52, 2; 561-565
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Genome size in Humulus lupulus L. and H. japonicus Siebold and Zucc. [Cannabaceae]
Autorzy:: Grabowska-Joachimiak, A
Sliwinska, E.
Pigula, M.
Skomra, U.
Joachimiak, A.J.
Powiązania:: https://bibliotekanauki.pl/articles/59081.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: genome size
Humulus lupulus
Humulus japonicus
Cannabaceae
flow cytometry
karyotype
sex chromosome
Opis:: We analysed chromosome lengths, karyotype structure, and nuclear DNA content (flow cytometry) in diploid (2n=20) and triploid (2n=30) European H. lupulus var. lupulus, American H. lupulus var. neomexicanus (2n=20) and Japanese ornamental hop, H. japonicus (F/2n=16; M/2n=17). Diploid female representatives of H. lupulus var. lupulus and H. l. var. neomexicanus differed in total length of the basal chromosome set (23.16 µm and 25.99 µm, respectively) and nuclear 2C DNA amount (5.598 pg and 6.064 pg) but showed similar karyotype structure. No deviation from the additivity, both in chromosome length and 2C DNA amount was evidenced in triploid monoecious H. lupulus (2n=30, XXY). H. japonicus showed different karyotype structure, smaller basal chromosome set (F/18.04 µm, M/20.66 µm) and lower nuclear DNA amount (F/3.208 pg and M/3.522 pg). There are first evaluations of nuclear genome size in diploid, not commercial representative of European H. lupulus var. lupulus and American H. lupulus var. neomexicanus and first attempt to determine the absolute male and female genome size in two Humulus species.
Źródło:: Acta Societatis Botanicorum Poloniae; 2006, 75, 3; 207-214
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg.
Autorzy:: Kolano, B
Siwinska, D.
Maluszynska, J.
Powiązania:: https://bibliotekanauki.pl/articles/56512.pdf
Data publikacji:: 2008
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: cytogenetic analysis
diploid
hexaploid
Chenopodium album
genome size
rDNA sequence
flow cytometry
polysomaty
Opis:: Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The Variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.
Źródło:: Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 293-298
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Induction of the multixenobiotic/multidrug resistance system in various cell lines in response to perfluorinated carboxylic acids
Autorzy:: Rusiecka, Izabela
Składanowski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1040751.pdf
Data publikacji:: 2008
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: multixenobiotic resistance
multidrug resistance
perfluorinated carboxylic acids
flow cytometry
ABCB1
ABCC1
Opis:: The multixenobiotic resistance (closely related to multidrug resistance) system controls transport across the plasma membrane as a defense against toxic molecules. Multixenobiotic resistance system consists of an efflux pump, ABCB1 (also named P-glycoprotein, P-gp), and/or a molecule of the ABCC family (also named multiple resistance associated protein, MRP). ABCB1 is able to increase efflux of many low-molecular foreign molecules. Measuring system induction may be used as a biomarker of cell/organism exposure to foreign substances. Various established cell lines were tested for constitutive and induced multixenobiotic resistance proteins by Western blotting immunodetection. The pumping function was indirectly assayed with Rhodamine B by visualization of cell fluorescence in the presence of verapamil. Changes in ABC proteins were measured by flow cytometry after exposition to various perfluorinated carboxylic acids. MCF7 and HeLa cells were found to contain the highest constitutive level of both ABCB1 and ABCC1. HEK293 exhibited much less ABCB1 and no activity of pumping out Rhodamine B. The pumping activity was found to be related to the amount of the cell-type specific 170 kDa ABCB1 protein. An 8-day exposure to 10-4 M perfluorononanoic acid resulted in about 2-2.5-fold increase of ABCB1 level. That was confirmed also for short times by flow cytometry of cells exposed to perfluorinated acids and its natural congeners. Both ABCB1- and ABCC1-related fluorescence increased along with the carbon chain in acids from C6 up to C9 and decreased for C10. Measuring of multixenobiotic resistance changes in vitro induced by chemicals may be a convenient test for screening for their potential toxicity.
Źródło:: Acta Biochimica Polonica; 2008, 55, 2; 329-337
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide
Autorzy:: Wojcik, R
Powiązania:: https://bibliotekanauki.pl/articles/32265.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: beta-1,3-1,6-D-glucan
cyclophosphamide
rat
phagocytic activity
oxidative metabolism
granulocyte
monocyte
flow cytometry
peripheral blood
immunosuppression
blood neutrophil
Opis:: The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 181-188
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Induction of the multixenobiotic/multidrug resistance system in HeLa cells in response to imidazolium ionic liquids
Autorzy:: Rusiecka, Izabela
Składanowski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1039912.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: multidrug resistance
multixenobiotic resistance
imidazolium ionic liquids
flow cytometry
ABCB1
ABCC1
Opis:: The multixenobiotic/multidrug resistance (MXR/MDR) system controls transport of foreign molecules across the plasma membrane as a preventive measure before toxicity becomes apparent. The system consists of an efflux pump, ABCB1, and/or a member of the ABCC family. Ionic liquids are broadly used solvents with several unique properties such as wide liquid range, negligible vapor pressure, good thermal and chemical stability and extraordinary dissolution properties for organic and inorganic compounds. Ionic liquids containing imidazolium ring are frequently used as solvents in drug synthesis. Constitutive and induced amounts of ABCB1 and ABCC1 proteins were estimated here by Western blotting and quantified by flow cytometry in HeLa cells exposed to three homologous 1-alkyl-3-methylimidazolium and one benzyl ring substituted salts. Aliphatic substituents in position 1 of the salts caused a weak toxicity but 1-benzyl ring was strongly toxic. An 8-day long treatment with 10-4 M 1-hexyl-3-methylimidazolium chloride resulted in an about 1.5-fold increase of ABCB1 level and over 2-fold increase of ABCC1 level. The amounts of both investigated ABC-proteins were linearly dependent on the length of the imidazolium ring side chain. Such distinctive changes of the amount of MXR/MDR proteins measured in cultured cells may be a useful marker when screening for potential toxicity of various chemicals.
Źródło:: Acta Biochimica Polonica; 2011, 58, 2; 187-192
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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