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Wyszukujesz frazę "expression pattern" wg kryterium: Temat


Wyświetlanie 1-6 z 6
Tytuł:
Characterization of a novel transcript variant of human STAU1 gene
Autorzy:
Fa-hui, Nie
Hai-feng, Yao
Rui-feng, Qi
Xin, Li
Cai-bin, Wu
Powiązania:
https://bibliotekanauki.pl/articles/1040700.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
expression pattern
STAU1
Opis:
Human STAU1 is one member of the family of double-stranded RNA (dsRNA)-binding proteins. It is thought to function in transporting mRNA, controlling translation and eliciting mRNA decay in neurons, and to function in infection of influenza virus and human immunodeficiency virus type 1 (HIV-1). Four transcripts coding two isoforms have been identified before. In this study, we have isolated a novel transcript of STAU1, coding a novel isoform that has six amino acids more (SFPLKQ) than isoform a. In order to examine the tissue distribution of this novel isoform, we have performed RT-PCR experiments and the analysis showed that it was highly expressed in heart, liver, kidney and pancreas.
Źródło:
Acta Biochimica Polonica; 2008, 55, 3; 473-478
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and characterization of novel human short-chain dehydrogenase/reductase SCDR10B which is highly expressed in the brain and acts as hydroxysteroid dehydrogenase*
Autorzy:
Huang, Chaoqun
Wan, Bo
Gao, Bo
Hexige, Saiyin
Yu, Long
Powiązania:
https://bibliotekanauki.pl/articles/1040586.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
expression pattern
clone
brain
hydroxysteroid dehydrogenase
Opis:
Hydroxysteroid dehydrogenase belongs to the subfamily of short-chain dehydrogenases/reductases (SDR), and 11-β-hydroxysteroid dehydrogenase catalyzes the interconversion of inactive glucocorticoids (cortisone in human, dehydrocorticosterone in rodents) and active glucocorticoids (cortisol in human, corticosterone in rodents). We report here the cloning and characterization of a novel human SDR gene SCDR10B which encodes a protein with similarity to 11β-hydroxysteroid dehydrogenase 1. SCDR10B was isolated from a human brain cDNA library, and was mapped to chromosome 19p13.3 by browsing the UCSC genomic database. It contains an ORF with a length of 858 bp, encoding a protein with a transmembrane helix and SDR domain. Its molecular mass and isoelectric point are predicted to be 30.8 kDa and 10.3 kDa, respectively. SCDR10B protein is highly conserved in mammals and fish. Phylogenetic tree analysis indicated that SCDR10B stands for a new subgroup in the 11β-hydroxysteroid dehydrogenase family. Northern blot analysis showed that SCDR10B was highly expressed in brain, and a strong expression signal was detected in hippocampal neurons by immunohistochemical analysis. RT-PCR and immunohistochemical analysis showed that SCDR10B was up-regulated in lung-cancer cell lines and human lung cancer. SCDR10B can catalyze the dehydrogenation of cortisol in the presence of NADP+, and therefore it is a hydroxysteroid dehydrogenase.
Źródło:
Acta Biochimica Polonica; 2009, 56, 2; 279-289
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physiological adaptations to osmotic stress and characterization of a polyethylene glycol-responsive gene in Braya humilis
Autorzy:
Lirong, W.
Pengshan, Z.
Xin, Z.
Xiaopeng, W.
Xiaofei, M.
Yi, L.
Powiązania:
https://bibliotekanauki.pl/articles/58230.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
physiological adaptation
osmotic stress
polyethylene glycol
gene
Braya humilis
drought
expression pattern
Opis:
Braya humilis (Brassicaceae) is a widely distributed plant in arid and semi-arid regions of northern Asia. This plant is well adapted to extremely arid conditions and is a promising candidate species to discover novel drought tolerance strategies. However, not much information about the mechanism(s) mediating drought resistance in this species is currently available. Therefore, the present study aimed to characterize the physiological traits and expression patterns of a polyethylene glycol (PEG)-responsive gene in B. humilis responding to different levels of osmotic stress induced by PEG-6000. Several important physiological parameters were examined, including the levels of relative water content, soluble protein, malondialdehyde, and antioxidant enzyme activity. A tolerance threshold between 20 and 30% PEG-6000 was identified for B. humilis. The water status and oxidative damage below this threshold were maintained at a relatively constant level during the 12 h of treatment. However, once the threshold was exceeded, the water status and oxidative damage were obviously affected after treatment for 4 h. The soluble protein results suggest that B. humilis maintains a vigorous resistance to osmotic stress and that it may play a greater role in osmotic regulation at late stages of stress. Moreover, superoxide dismutase and catalase may be important at preventing oxidative damage in plants at early stages of stress, while peroxidase may be more involved in some biological processes that resist osmotic stress at the late stage, especially in severely damaged plants. Furthermore, a PEG-responsive gene, BhCIPK12, was identified by differential display reverse transcription-polymerase chain reaction (PCR), cloned, and characterized by quantitative real-time PCR. We hypothesized that this gene may play an important role in mediating osmotic stress or drought resistance in plants. Altogether, these results provide valuable insights into the mechanism(s) mediating drought tolerance in B. humilis.
Źródło:
Acta Societatis Botanicorum Poloniae; 2016, 85, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ERF022 controls somatic embryogenesis in Arabidopsis via ethylene-related mechanism
Autorzy:
Nowak, K.
Gaj, M.D.
Powiązania:
https://bibliotekanauki.pl/articles/80300.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
gene encoding
transcription factor
somatic embryogenesis
ERF022 gene
Arabidopsis
expression pattern
tf gene
plant stress
ethylene biosynthesis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Calcium and MAP kinase signaling in PAMP-triggered immunity
Autorzy:
Scheel, D.
Powiązania:
https://bibliotekanauki.pl/articles/80214.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
pathogen-associated molecular pattern
plasma membrane
peptidoglycan
lipopolysaccharide
mitogen-activated kinase signalling
reactive oxygen species
gene expression
calcium
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A calcium-dependent protein kinase-NADPH oxidase activation circuit in rapid defense signal propagation
Autorzy:
Seybold, H.
Dubiella, U.
Lassing, R.
Schulze, W.
Romeis, T.
Powiązania:
https://bibliotekanauki.pl/articles/80432.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
calcium-dependent protein kinase
phosphorylation
NADPH oxidase
pathogen-associated molecular pattern
innate immune system
plant
phytohormone
gene expression
reactive oxygen species
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-6 z 6

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