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Tytuł:
Analiza wstępowania szpitalnych zakażeń układu moczowego w 10-letniej obserwacji oddziału chorób wewnętrznych i nefrologii w Małopolsce
Autorzy:
Wałaszek, Marta
Kołpa, Małgorzata
Wolak, Zdzisław
Sydor, Antoni
Pelczar, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2097675.pdf
Data publikacji:
2017-06-30
Wydawca:
Państwowa Wyższa Szkoła Zawodowa w Tarnowie
Tematy:
ddział chorób wewnętrznych
zakażenie szpitalne
Healthcare – Associated Infections (HAI)
szpitalne zakażenie układu moczowego (UTI)
E. coli
P. aeruginosa
internal medicine department
hospital-acquired infections
healthcare associated infections
urinary tract infections
Opis:
Wstęp: Zakażenia szpitalne są jednym z poważniejszych zagrożeń zdrowia pacjenta podczas hospitalizacji. Wśród nich zakażenia szpitalne HAI (Healthcare – Associated Infections). Najczęściej występującą formą są szpitalne zakażenia układu moczowego (UTI – Urinary Tract Infection). Cel: W celu zbadania częstości występowania szpitalnych zakażeń układu moczowego poddano analizie strukturę zakażeń szpitalnych występujących u pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii Szpitala Wojewódzkiego im. Św. Łukasza w Tarnowie. Materiał i metody: Analizowano dane dotyczące 13 965 pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii w latach od 2006 do 2015. W analizie materiału wykorzystano standardowe metody epidemiologiczne i ujednolicone definicje zakażeń szpitalnych wydane przez ECDC (European Center for Disease Prevention and Control) oraz CDC (Centers for Disease Control and Prevention). Wyniki: Wykryto 237 szpitalnych UTI co stanowiło 33% wszystkich zakażeń szpitalnych wykrytych w badanym oddziale. Zachorowalność wynosiła 1,7%, w tym: 1,5% dla potwierdzonych mikrobiologicznie zakażeń (UTI-A: Microbiologically Confirmed Symptomatic UTI) i 0,2% bez potwierdzenia mikrobiologicznego (UTI-B: Not Microbiologically Confirmed Symptomatic UTI). Współczynnik gęstości zachorowań na 1000 osobodni pobytu (ID – density incidence /1000 osobodni pobytu) wynosił 0,2/1000. Rozpoznano 168 przypadków zakażeń UTI związanych z cewnikiem moczowym i 69 przypadków bez cewnika moczowego. Współczynnik gęstości zachorowań dla UTI z cewnikiem moczowym wynosił 3,3 na 1000 osobodni z cewnikiem moczowym. Wśród czynników etiologicznych, które izolowano z materiałów pochodzących od pacjentów z zakażeniem układu moczowego (UTI) dominowały: Escherichia coli 63 (29%), Enterococcus spp. 37 (16%), Klebsiella spp. 23 (11%). Wnioski: Dziesięcioletnia obserwacja szpitalnych UTI występujących w oddziale chorób wewnętrznych i nefrologii wykazała możliwość przeprowadzenia bardzo dokładnej analizy epidemiologicznej tych zakażeń. Porównanie gęstości występowania szpitalnych UTI uzyskane w badanym oddziale z odnotowaną w programach CDC pozwala wnioskować, iż sytuacja epidemiologiczna w badanym oddziale nie odbiega znacząco od sytuacji w innych krajach.
Introduction: Hospital-acquired infections are one of the most serious health threats during a patient’s stay in hospital, including healthcare associated infections (HAI). The most typical form of hospital-acquired infections is urinary tract infection (UTI). Objective: To examine the frequency of appearing UTIs, the structure of UTIs in in-patients in the department of internal medicine and nephrology at Saint Lucas’s general hospital in Tarnów was analysed. Materials and methods: Data analysis of 13 965 in-patients staying in the department of internal medicine and nephrology from 2006 to 2015 was carried out. To investigate these data epidemiological methods and standard definitions of hospital- acquired infections issued by European Center for Disease Prevention as well as Control and Centers for Disease Control and Prevention were used. Results: 237 hospital-acquired UTIs were revealed, which is 33% of all UTIs revealed in the investigated ward. The UTI incidence rate was 1.7% including 1.5% for microbiologically confirmed symptomatic UTIs and 0.2% not microbiologically confirmed symptomatic UTIs. The incidence density rate per 1 000 person-days was 0.2 over 1 000. The number of revealed catheter-related cases was 168, and not catheter-related cases – 69. The incidence density rate of UTIs associated with urinary catheters was 3.3 per 1 000 person-days. The dominant etiological factors, which were taken to be detected from the infected patients’ specimens, were: Escherichia coli 63 (29%), Enterococcus spp. 37(16%), Klebsiella spp. 23 (11%). Conclusions: A 10 year observation of UTIs, which have appeared in the department of internal medicine and nephrology, allowed to conduct the accurate analysis of these infections. The comparison of urinary tract infection rates done in the investigated ward, and recorded in the Research Participation Programs at the Centers for Disease Control and Prevention (CDC), allows to draw the conclusion that the presented epidemiological situation does not differ significantly from other countries.
Źródło:
Health Promotion & Physical Activity; 2017, 2, 3; 141-154
2544-9117
Pojawia się w:
Health Promotion & Physical Activity
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia
Autorzy:
Geta, Kindu
Kebede, Ameha
Chemedissa, Meseret
Powiązania:
https://bibliotekanauki.pl/articles/1076786.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antibiotics
Antimicrobial resistance
B. cereus
Debre Markos
E. coli
Enterobacter
Fruit juices
Klebsiella
Pathogens
Ps. aeruginosa
Salmonella
Shigella
Staphylococcus aureus
Opis:
The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.
Źródło:
World News of Natural Sciences; 2019, 24; 365-371
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of microbiological quality of water in the Nowohucki Reservoir with particular regard to microorganisms potentially dangerous to humans
Autorzy:
Wolny-Koładka, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1035482.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
"E. coli"
"E. faecalis"
"Nowohucki Reservoir"
"mesophilic bacteria"
"microbiological contamination of water"
"surface water"
Opis:
Introduction. This study was aimed to assess the microbiological quality of water in the Nowohucki Reservoir (Kraków, Poland) as well as to determine whether its waters contain microorganisms potentially dangerous from an epidemiological point of view. Material and methods. Microbiological analyses included the determination of the number of mesophilic and psychrophilic bacteria, coliforms, fecal E. coli, as well as E. faecalis, C. perfringens, Staphylococcus spp. and Salmonella spp.. Water samples were collected 4 times per year on April 27th 2015 (spring), July 10th 2015 (summer), October 12th 2015 (autumn) and December 29th 2015 (winter) at 5 points within the area of the reservoir. Water and air temperature was measured onsite. Results. It was found that the prevalence of the analyzed microorganisms was affected by changing water and air temperature as well as by using this reservoir during holiday season for swimming purposes by local residents. All analyzed microbiological indicators of poor water quality were found in the analyzed water samples, which may pose a potential health risk to people swimming in the considered reservoir. Conclusions. From an epidemiological point of view, it is reasonable to include the Nowohucki Reservoir into a constant sanitary monitoring programme.
Wstęp. Badania miały na celu ocenę stanu mikrobiologicznego wód Zalewu w Nowej Hucie (Kraków, Polska) a także określenie, czy w zbiorniku występują drobnoustroje potencjalnie niebezpieczne z epidemiologicznego punktu widzenia. Materiał i metody. Analizy mikrobiologiczne obejmowały określenie liczebności bakterii mezofilnych, psychrofilnych, bakterii grupy coli, form kałowych E. coli, a także E. faecalis, C. perfringens, Staphylococcus spp. i Salmonella spp.. Próbki wód pobierane były 4 razy w roku w następujących terminach: 27.04.2015 r. (wiosna), 10.07.2015 r. (lato), 12.10.2015 r. (jesień) i 29.12.2015 r. (zima) w 5 punktach na terenie zalewu. Ponadto, w każdym punkcie pomiarowym mierzono temperaturę wody oraz powietrza. Wyniki. Stwierdzono, że na liczebność badanych drobnoustrojów wpływała zmieniająca się temperatura wody i powietrza oraz użytkowanie w sezonie wakacyjnym zalewu w charakterze kąpieliska przez okolicznych mieszkańców. W pobranych próbkach zidentyfikowano wszystkie badane wskaźniki czystości mikrobiologicznej wód, które stanowią potencjalne zagrożenie dla kąpiących się w zbiorniku ludzi. Wnioski. Z epidemiologicznego punktu widzenia zasadnym jest objęcie wód Zalewu w Nowej Hucie stałym monitoringiem sanitarnym.
Źródło:
Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 4; 19-26
1505-7054
2084-6312
Pojawia się w:
Medycyna Środowiskowa - Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose
Autorzy:
Sarabia-Sainz, Andre-i
Ramos-Clamont, Gabriela
Winzerling, Joy
Vázquez-Moreno, Luz
Powiązania:
https://bibliotekanauki.pl/articles/1039957.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
neoglycans
bacterial recognition
E. coli K88 adhesion
glycation of serum albumin
Opis:
Recently, glyco-therapy is proposed to prevent the interaction of bacterial lectins with host ligands (glycoconjugates). This interaction represents the first step in infection. Neoglycans referred to as PSA-Lac (PSA-Glu (β1-4) Gal) were obtained by conjugation of porcine serum albumin (PSA) with lactose at 80 °C, 100 °C and 120 ºC. Characterization studies of the products showed that PSA could contain 1, 38 or 41 added lactoses, depending on the reaction temperature. These neoglycans were approximately 10 times more glycated than PSA-Lac obtained in previous work. Lactose conjugation occurred only at lysines and PSA-Lac contained terminal galactoses as confirmed by Ricinus communis lectin recognition. Furthermore, Escherichia coli K88+, K88ab, K88ac and K88ad adhesins showed affinity toward all PSA-Lac neoglycans, and the most effective was the PSA-Lac obtained after 100 ºC treatment. In vitro, this neoglycan partially inhibited the adhesion of E. coli K88+ to piglet mucin (its natural ligand). These results provide support for the hypothesis that glycated proteins can be used as an alternative for bioactive compounds for disease prevention.
Źródło:
Acta Biochimica Polonica; 2011, 58, 1; 95-100
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CEA-negative glioblastoma and melanoma cells are sensitive to cytosine deaminase/5-fluorocytosine therapy directed by the carcinoembryonic antigen promoter.
Autorzy:
Dąbrowska, Anna
Szary, Jarosław
Kowalczuk, Małgorzata
Szala, Stanisław
Ugorski, Maciej
Powiązania:
https://bibliotekanauki.pl/articles/1041550.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CEA promoter
E. coli cytosine deaminase
gene-directed enzyme prodrug therapy
Opis:
Recent studies have suggested that carcinoembryonic antigen (CEA)-promoter sequences are active only in CEA-positive cells, filing in the criteria for tumor specific targeting of suicide genes. However, the present study on gene therapy of colon cancer and cell-specificity of CEA promoter, provide evidence that CEA-positive and CEA-negative cells transfected with E. coli cytosine deaminase (CD) gene under the control of CEA promotor sequence are sensitive to enzyme/pro-drug therapy with 5-fluorocytosine (5-FC). Individual clones derived from the CEA-negative cell lines: melanoma Hs294T and glioblastoma T98G after transfection with CD differed profoundly in their sensitivity to 5-FC. The IC50 values for several clones of the CEA-negative cells were almost the same as for CEA-positive colon cancer cells. Such 5-FC-sensitive clones derived from the population of CEA-negative cells, present even in small number, because of the very effective bystender effect of this enzyme/pro-drug system can cause severe problems during therapy by efficiently killing surrounding normal cells. Safety is the major issue in gene therapy. Our data suggest that the safety of gene-directed enzyme pro-drug therapy (GDEPT) with CEA promoter driven expression of therapeutic genes is not so obvious as it has originally been claimed.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 723-732
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cloning of a Rift Valley Fever fusion gene in a plant virus derived replicon vector
Autorzy:
Omosimua, Rebecca Oziohu
Iyappan, Gowtham
Obembe, Olawole
Ogunkanmi, Adebayo
Sathishkumar, Ramalingam
Powiązania:
https://bibliotekanauki.pl/articles/1193478.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
DNA Cloning
Escherichia coli DH5α cells
PBYR2e3K2Mc-GFP
PBYR2e3K2Mc-RVFV
RVF disease
RVFV fusion gene
bean yellow dwarf derived vector
emerging disease
geminivirus vector
zoonotic disease
Opis:
Rift Valley fever (RVF) disease is an emerging viral zoonotic disease caused by Rift Valley fever virus (RVFV). RVF disease is seemingly becoming more severe in endemic populations and in areas of new outbreak. RVF disease is listed by WHO as requiring urgent research and development attention. There is no therapeutics or licensed vaccine for human use in the case of an eventual outbreak. There is a need to clone RVFV genes in cloning vectors which may be useful to produce antigens in plant cells or may be tested directly as DNA vaccines. A synthesized Rift Valley fever virus fusion gene was cloned in a bean yellow dwarf virus derived replicon vector; PBYR2e3K2Mc-GFP by removing and replacing the GFP gene. The cloned PBYR2e3K2Mc-RVFV fusion gene was confirmed by PCR, restriction digestion and DNA sequencing. This cloned RVFV fusion gene in plant virus vector can be used for subsequent protein expression in plants cells or used directly tested as DNA vaccines in future studies.
Źródło:
World Scientific News; 2021, 158; 159-172
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
COMPARATIVE IN VITRO STUDIES OF FURAZIDIN AND NITROFURANTOIN ACTIVITIES AGAINST COMMON UROPATHOGENS INCLUDING MULTIDRUG-RESISTANT STRAINS OF E. COLI AND S. AUREUS
Autorzy:
Klesiewicz, Karolina
Karczewska, Elżbieta
Nowak, Paweł
Mrowiec, Paulina M.
Skiba-Kurek, Iwona
Białecka, Joanna
Majka, Zbigniew
Berdzik-Kalarus, Sylwia
Budak, Alicja
Zajdel, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/895689.pdf
Data publikacji:
2018-06-30
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
ESBL-positive E. coli
MRSA
furazidin
nitrofurantoin
urinary tract infections
Opis:
Abstract: Urinary tract infections caused by wide range of pathogens including gram-negative and gram-positive bacteria as well as fungi are a severe public health problem. The predominant causative agent of both uncomplicated and complicated urinary tract infections is Escherichia coli. In an era of increasing bacterial resistance to antimicrobial agents and a high prevalence of multidrug-resistant (MDR) strains in community and hospital acquired infections, the re-evaluation of older generations of antimicrobial agents, such as nitrofuran derivatives, seems to be a reasonable approach. The aim of the study was to evaluate furazidin activity against common uropathogens in comparison to nitrofurantoin and other selected antimicrobial agents, routinely used in the treatment of urinary tract infections. Furazidin exhibited lower MICs than nitrofurantoin when tested against gram-negative and gram-positive bacteria including clinical MDR E. coli and methicillin-resistant Staphylococcus aureus. The MICs for furazidin ranged from 4 to 64 mg/L for Enterobacteriaceae strains, from 2 to 4 mg/L for gram-positive cocci, and 0.5 mg/L for anaerobic bacteria. The MICs for nitrofurantoin ranged from 16 to 64 mg/L for Enterobacteriaceae strains, from 8 to 64 mg/L for gram-positive cocci, and 4 mg/L for anaerobic bacteria. In addition, both nitrofurans displayed better activity against the tested bacterial strains than ciprofloxacin, fosfomycin, trimethoprim and co-trimoxazole. Nitrofuran derivatives displayed higher antimicrobial activity than other antimicrobial agents regardless of bacteria species or resistance mechanism.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 3
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli
Autorzy:
Bzdzion, Lukasz
Krezel, Hanna
Wrzeszcz, Karol
Grzegorek, Irmina
Nowinska, Katarzyna
Chodaczek, Grzegorz
Swietnicki, Wieslaw
Powiązania:
https://bibliotekanauki.pl/articles/1038684.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Escherichia coli (E. coli)
type III secretion system (T3SS)
enzyme inhibitor
small molecule
Opis:
Enteropathogenic E. coli (EPEC) is a human pathogen using type III secretion system for delivery of proteins directly into the human host. The system contains a single ATPase, EscN, which is essential for uncoupling of proteins from their complexes with chaperones before the delivery. The structure of EscN ATPase (PDB code: 2obm) was used to screen computationally for small molecule inhibitors blocking its active site. Two lead candidates were examined but only one, Compound 54, was selected for further optimization. After extended QSAR optimization, two derivatives were found to be competitive inhibitors of EscN capable of blocking ATPase activity with a Ki below 50 µM. One candidate, WEN05-03, with a Ki=16±2 µM, was also minimally toxic to mammalian cells as determined by other assays. In the cell infection model of HeLa cells with EPEC, Compound WEN05-03 completely blocked actin cluster formation at 100 µM concentration, when analyzed by confocal microscopy. The second best inhibitor of EscN ATPase activity was WEN04-34 with a Ki=46±2 µM. However, the compound was highly toxic to the BALB/3T3 cell line. In summary, the work identifies a compound blocking bacterial ATPase in its active site without causing cellular toxicity to the host cells. It is the first report showing feasibility of using bacterial virulence system ATPase as a target for safe, non-toxic compounds and offering a proof-of-concept for non-antibiotic alternatives.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 49-63
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and enumeration of Enteric bacteria associated with food handlers and surfaces of food manufacturing industry located in Hub city, Pakistan
Autorzy:
Ali, Razim
Hayat, Amir
Fatima, Madiha
Noman, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1182928.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
enterobacteriaceae
foodborne microbes
hygiene
surface contamination
food handlers
e. coli
sanitation
Opis:
Foodborne microorganisms harbor and adheres itself to the food material and surrounding surfaces for a long time and influence the food quality and consumers health. Among these microbes the presence of Enteric indicator bacteria in food premises confers the indication of entero-pathogens, i.e. E. coli, Salmonella, Shigella and Campylobacters that could cause severe systemic infections in consumers. In this study, the hygienic status of confectionery and supplementary food processing facility was evaluated. A total of 10497 examinations were performed on 3499 swab samples collected from food premises and handlers for the analysis of Enteric indicator bacteria. From swabs, 1277 (12.2%) isolates were identified in which Enterobacteriaceae were found with higher frequency 604 (47.3%) followed by Coliforms 293 (30.8%) and Escherichia coli 280 (21.9%) respectively. The mean count (CFU/cm2) was found maximum for plain surfaces (floors, walls and door), while the lowest was for equipment and machinery. Overall isolates percent prevalence was determined where Enterobacteriaceae were 47%, Coliforms 31% and Escherichia coli 22%. Majority of the floor surfaces were highly contaminated, where washing and sanitation practices were observed to be inappropriate. Worker hygiene status was lacking essential food safety and hygiene standards. In general, the Enteric bacteria were found with higher ratio, that could affect the food quality and quantity both to a greater extent with some influences on consumers health.
Źródło:
World Scientific News; 2016, 49, 2; 192-203
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of SOS-induced Pol II, Pol IV, and Pol V DNA polymerases on UV-induced mutagenesis and MFD repair in Escherichia coli cells.
Autorzy:
Wrzesiński, Michał
Nowosielska, Anetta
Nieminuszczy, Jadwiga
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1041471.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
MFD
polB
UV induced mutagenesis
E. coli
dinB
mutD5
umuDC
Opis:
Irradiation of organisms with UV light produces genotoxic and mutagenic lesions in DNA. Replication through these lesions (translesion DNA synthesis, TSL) in Escherichia coli requires polymerase V (Pol V) and polymerase III (Pol III) holoenzyme. However, some evidence indicates that in the absence of Pol V, and with Pol III inactivated in its proofreading activity by the mutD5 mutation, efficient TSL takes place. The aim of this work was to estimate the involvement of SOS-inducible DNA polymerases, Pol II, Pol IV and Pol V, in UV mutagenesis and in mutation frequency decline (MFD), a mechanism of repair of UV-induced damage to DNA under conditions of arrested protein synthesis. Using the argE3→Arg+ reversion to prototrophy system in E. coli AB1157, we found that the umuDC-encoded Pol V is the only SOS-inducible polymerase required for UV mutagenesis, since in its absence the level of Arg+ revertants is extremely low and independent of Pol II and/or Pol IV. The low level of UV-induced Arg+ revertants observed in the AB1157mutD5ΔumuDC strain indicates that under conditions of disturbed proofreading activity of Pol III and lack of Pol V, UV-induced lesions are bypassed without inducing mutations. The presented results also indicate that Pol V may provide substrates for MFD repair; moreover, we suggest that only those DNA lesions which result from umuDC-directed UV mutagenesis are subject to MFD repair.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 139-147
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Escherichia coli in sewage sludge - detection method
Escherichia coli w osadach ściekowych - metoda wykrywania
Autorzy:
Machnicka, A.
Powiązania:
https://bibliotekanauki.pl/articles/106338.pdf
Data publikacji:
2014
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
E.coli
sewage sludge
enrichment of Escherichia coli
detection of Escherichia coli
E. coli
osady ściekowe
namnażanie Escherichia coli
wykrywanie Escherichia coli
Opis:
Escherichia coli is Gram-negative optionally anaerobic roads which belongs to Enterobacteriaceae family. Includes in a physiological bacterial flora of human and warm-blooded animals large intestine. Escherichia coli is being met in abiotic elements of the environment so as waters, wastewater, sewage sludge, soil and the food. This bacterium is showing the pathogenicity in named terms for the peoples, triggering diseases mainly: gastrointestinal tract and urinary. Quality and quantitative proposed detections method of the bacteria E. coli contains five/six steps: - appointment dry suspended solid, - preparation averaged, test of sample and resuscitation of bacteria, - making dilutions, - enrichment and differentiation in chromogenic-selective medium, - enumerating the amount of cfu E. coli in 1 g of a dry weight, - optionally, the biochemical identification.
Escherichia coli jest Gram-negatywną, względnie beztlenową pałeczką należącą do rodziny Enterobacteriaceae. Wchodzi w skład fizjologicznej flory bakteryjnej jelita grubego człowieka oraz zwierząt stałocieplnych. Spotyka się ją w elementach abiotycznych środowiska, takich jak wody, ścieki, osady ściekowe, gleba i żywność. Bakteria ta w określonych warunkach wykazuje chorobotwórczość dla człowieka, wywołując głównie schorzenia układów pokarmowego i moczowego. Proponowana metoda jakościowa i ilościowa wykrywania bakterii E. coli zawiera pięć/sześć etapów: - oznaczenie suchej masy osadu, - przygotowanie próbki uśrednionej i badawczej oraz przywrócenie bakteriom aktywności fizjologicznej, - wykonanie rozcieńczeń, - namnażanie i identyfikacja na podłożu chromogennym-selektywnym, - wyliczenie ilości jtk E. coli w 1 g suchej masy osadu, - opcjonalnie identyfikacja biochemiczna.
Źródło:
Chemistry-Didactics-Ecology-Metrology; 2014, 19, 1-2; 79-85
2084-4506
Pojawia się w:
Chemistry-Didactics-Ecology-Metrology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of significance of AlkB and AlkA proteins in DNA repair in Escherichia coli model
Autorzy:
Sokołowska, B.
Maciejewska, A. M.
Jóźwik, A.
Kuśmierek, J. T.
Powiązania:
https://bibliotekanauki.pl/articles/333316.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Śląski. Wydział Informatyki i Nauki o Materiałach. Instytut Informatyki. Zakład Systemów Komputerowych
Tematy:
rozpoznawanie obrazów
klasyfikator najbliższych sąsiadów
analiza cech pracy odbiornika
naprawa DNA
adaptacyjna odpowiedź
powstawanie mutacji
bakterie e.coli
pattern recognition
fuzzy k-NN classifier
ROC analysis
DNA repair
adaptive response
mutagenesis
AlkB dioxygenase
AlkA glycosylase
E.coli
Opis:
The paper concerns estimation of significance of differences of mutagenesis level between the wild-type strain (wt) and its derivatives which differ in DNA repair ability, namely alkA and alkB strain, devoided AlkA glycosylase and AlkB dioxygenase activity, respectively. The strains were analyzed for their ability to repair 1,N6-ethenoadenine (εA) - chloroacetaldehyde adduct to DNA. The analysis was done using classical statistical and pattern recognition methods. The obtained results confirmed that AlkB dioxygenase plays the most important role in εA repair in E. coli in the experimental modeling.
Źródło:
Journal of Medical Informatics & Technologies; 2011, 17; 321-326
1642-6037
Pojawia się w:
Journal of Medical Informatics & Technologies
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system
Autorzy:
Mazurkiewicz-Pisarek, Anna
Mazurkiewicz, Alina
Mikiewicz, Diana
Baran, Piotr
Ciach, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/16706242.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
factor VIII
hemophilia type A
recombinant coagulation factor VIII
prokaryotic expression system
E. coli
recombinant protein production system
Opis:
In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 3; 247-262
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of bacteria from soil sample of Tamil Nadu and their in vitro interaction
Autorzy:
Sankari, D.
Khusro, Ameer
Aarti, Chirom
Powiązania:
https://bibliotekanauki.pl/articles/1031683.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antagonism
B. subtilis
CFU
E. coli
Interaction
P. aeruginosa
Opis:
The present study was investigated to determine the competitive interaction between Gram positive (B. subtilis) and Gram negative (P. aeruginosa and E. coli) bacteria in vitro. Bacteria of interest were isolated from soil sample and were identified through morphological and biochemical tests. Bacterial cultures were grown alone and as co- culture in test tubes containing sterile broth. Bacterial growth and their growth inhibition in co- culture tubes were analyzed till 96 h through spectrophotometric assay and colony forming unit (CFU) assay. Spectrophotometric analysis and CFU/mL assay showed that the effect of B. subtilis on P. aeruginosa and E. coli in co- culture tubes was growth inhibitory. This is accompanied by the reduction in absorbance value and CFU/mL of the co-culture tubes. The bactericidal activity of B. subtilis was measured on P. aeruginosa and E. coli using agar well diffusion method. B. subtilis showed zone of inhibition of 8 mm and 6 mm against P. aeruginosa and E. coli, respectively. The antagonistic activity test of B. subtilis favoured the spectrophotometric and colony forming assay results. These results suggest that B. subtilis develop adaptational pathways by extracellular signaling molecules and antibacterial factors in order to compete with bacterial neighbors.
Źródło:
World News of Natural Sciences; 2020, 28; 67-75
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LBP gene methylation involved in mRNA expression and resistance to E. coli F18 in weaned piglets
Autorzy:
Gan, L.N.
Bao, W.B.
Wu, S.L.
Qin, W.Y.
Sun, L.
Zhao, C.X.
Powiązania:
https://bibliotekanauki.pl/articles/2087872.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
LBP gene
methylation analysis of the LBP region
E.coli F18 strain
weaned piglets
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 643-650
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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