- Tytuł:
- Protective antigen domain 4 of Bacillus anthracis as a candidate for use as vaccine for anthrax
- Autorzy:
-
Żakowska, D.
Graniak, G.
Rutyna, P.
Naylor, K.
Glowacka, P.
Niemcewicz, M. - Powiązania:
- https://bibliotekanauki.pl/articles/2085049.pdf
- Data publikacji:
- 2019
- Wydawca:
- Instytut Medycyny Wsi
- Tematy:
-
Bacillus anthracis
cloning pag gene
domain 4
protective antigen
protein
recombinant
vaccine - Opis:
- Existing research for using the protective antigen (PA) of Bacillus anthracis as a vaccine component shows that protection against anthrax may be obtained using fragments of this protein. The aim of the research is to check whether the selected protein fragment of the protective antigen (domain 4) encoded by an appropriate nucleotide sequence of gene pag of B. anthracis, was expressed in the bacterial system of E. coli. In order to examine the selected sequence of the pag gene, a PCR reaction and a highly effective TOPO cloning strategy were used, followed by purification of the recombinant proteins and their detection by a western-blot method. In the planning of the PA4 antigen expression a higher level of effectiveness in production of small protein – domain 4 – was anticipated. As a result, the 139 amino acids protein fragment of B. anthracis PA (domain 4) was isolated. The research may have found the basis for in vivo research aimed at finding potential anthrax vaccine components.
- Źródło:
-
Annals of Agricultural and Environmental Medicine; 2019, 26, 3; 392-395
1232-1966 - Pojawia się w:
- Annals of Agricultural and Environmental Medicine
- Dostawca treści:
- Biblioteka Nauki
Artykuł