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Tytuł:
Określenie pochodzenia wyłączonych drzewostanów nasiennych sosny rychtalskiej (Pinus sylvestris L.) z wykorzystaniem markerów mikrosatelitarnych
Determination of the origin of the rychtal Scots pine (Pinus sylvestris L.) seed tree stands using microsatellite markers
Autorzy:
Wójkiewicz, B.
Żukowska, W.B.
Urbaniak, L.
Kowalczyk, J.
Litkowiec, M.
Lewandowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/985719.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
sosna zwyczajna
Pinus sylvestris
sosna rychtalska
drzewostany nasienne
pochodzenie roslin
zmiennosc genetyczna
analiza DNA
markery mikrosatelitarne
scots pine
genetic variation
gene pool
genetic structure
ssr markers
Opis:
The rychtal pine is one of the most valuable ecotypes of Scots pine (Pinus sylvestris L.) approved for the breeding purposes in Poland. However, it occupies stands typical for oaks and beeches as shown by the compatibility analysis of species composition in relation to the habitat type in which they occur. Such result raises some doubts in terms of the naturalness of the rychtal pine and calls its history and origin into question. In the present study, we used the set of nuclear microsatellite markers to characterize and compare the gene pool composition of the selected seed tree stands of the rychtal pine with 200−year−old pine trees which grow at the Syców Forest District (SW Poland). We aimed to know to what extent the set of alleles specified for the group of the oldest trees from natural habitats is represented in the younger forest tree stands of the rychtal pine. The analysis of molecular variance (AMOVA) and clustering analysis showed that the gene pool of the studied pine populations was homogenous (FST=0,02%, K=1). The parameters of genetic variation were similar for all populations except for the mean number of alleles. On average, 25 new alleles were found in two rychtal pine seed tree stands as compared to the set of alleles found in the group of old pine trees. However, all alleles defined for old pines were also present in the gene pool of younger rychtal pine forest stands. The differences in the gene pool richness result most likely from quite high differences in the number of individuals analyzed from each population. In conclusion, our results indicate the common origin of the studied Scots pine populations.
Źródło:
Sylwan; 2019, 163, 08; 637-644
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Location of markers of aluminium tolerance genes on rye chromosomes (Secale cereale L.)
Autorzy:
Wiśniewska, Iwona
Rafalski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/2199029.pdf
Data publikacji:
2006-06-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
aluminium tolerance
chromosomal location
DNA-DNA hybridisation
PCR markers
wheat/rye addition lines
Opis:
The aim of presented work was to identify of PCR amplifiedDNAfragments differentiating aluminium tolerant and sensitive forms of rye and to locate the markers on rye chromosomes. For identification of markers, the PCR system with semi-specific primers targeting intron-exon sequences of plant genes was applied. The modified method of bulked segregant analysis was used. The pooled DNAs of two or three F2 segregating populations were screened together with DNA of their parental inbred lines. Potential marker of tolerance gene was located on rye chromosomes using wheat/rye (Chinese Spring/Blanco) additional lines. The specific probes obtained from DNA fragments differentiating sensitive and tolerant forms of rye were hybridized to PCR amplified DNA fragments of sensitive and tolerant forms of rye and the set of wheat/rye addition lines. Independently of the method of digoxygenin labelling (primer extension or Taq polymerase reaction), the probes obtained showed similar hybridization patterns. The results of hybridisation of 21 probes prepared from 12 DNA fragments confirmed connection of selected DNA fragments with to aluminium tolerance or sensitivity. Most of these DNA fragments originated from tolerant forms of rye. Using this method it was possible to locate eight DNA fragments on rye chromosomes. Three DNA fragments hybridised to chromosome 4R, two DNA fragments to chromosome 6R and single DNA fragments to chromosomes 1R, 2R and 3R. Four DNA fragments indicating clear relationship with character studied were not located on particular chromosomes using this set of wheat/rye addition lines. Hybridisation of probes prepared from four DNA fragments revealed length polymorphism. Probes prepared from two DNA fragments were characterised as dominant markers. In other cases the type of marker (dominant/codominat) was not fully documented.
Źródło:
Plant Breeding and Seed Science; 2006, 53; 43-52
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ metod izolacji DNA na podobieństwo genetyczne linii pszenicy ozimej (Triticum aestivum L.) przy zastosowaniu techniki RAPD-Pcr
The effect of DNA isolation methods on genetic similarity of winter wheat (Triticum aestivum L.) lines using RAPD-PCR techniques
Autorzy:
Tomkowiak, A.
Kurasiak-Popowska, D.
Weigt, D.
Mikołajczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/1366472.pdf
Data publikacji:
2015
Wydawca:
Centralny Ośrodek Badawczo-Rozwojowy Aparatury Badawczej i Dydaktycznej, COBRABiD
Tematy:
izolacja DNA
pszenica ozima
polimorfizm markerów RAPD
isolation of DNA
winter wheat
polymorphism of RAPD markers
Opis:
Celem badań było porównanie wpływu 4 metod izolacji DNA na wynik analizy markerami molekularnymi RAPD-PCR (ang. Random Amplified Polymorphic DNA - Polymerase Chain Reaction) linii pszenicy ozimej. Przeprowadzono izolację DNA metodą Thomsona i Henry'ego, metodą kolumienkową przy pomocy kitu Quiagen DNeasy Plant Kit, przy pomocy kitu Genomic Mini AX Plant firmy A&A Biotechnology oraz zestawu Maxwell® 16 LEV Plant DNA Kit firmy Promega.Po izolacji przeprowadzono analizy molekularne RAPD-PCR, które wykorzystano jako wskaźnik służący do oceny metod izolacji DNA. Najlepszą metodą izolacji okazała się metoda z wykorzystaniem Quiagen DNeasy Plant Kit. Jakość DNA otrzymanego po izolacji tym kitem była najwyższa, a otrzymane elektroforogramy były najbardziej czytelne. Jest to jednak metoda izolacji DNA najdłuższa i najdroższa, co ogranicza jej wykorzystanie przy dużej liczbie badanych genotypów. Dobrą metodą izolacji DNA ze względu na jakość otrzymanych obrazów elektroforetycznych była metoda izolacji kitem Genomic Mini AX Plant. Metodą szybką i tanią okazała się metoda Thomsona i Henry'ego, niestety mniej wydajną w porównaniu do poprzednich. Metodą o największym zróżnicowaniu ilości otrzymanego DNA była izolacja z wykorzystaniem zestawu Maxwell.
The aim of this study was to compare the effect of 4 DNA isolation methods on the results of analyses using molecular markers of Random Amplified Polymorphic DNA - Polymerase Chain Reaction (RAPD-PCR) in winter wheat lines. DNA was isolated using the method proposed by Thomson and Henry, the column method with a Quiagen DNeasy Plant Kit, a Genomic Mini AX Plant Kit by A&A Biotechnology and the Maxwell® 16 LEV Plant DNA Kit by Promega. After isolation RAPD-PCR analyses were carried out. The molecular study was an evaluation indicator of the DNA isolation methods. The best isolation method was that using the Quiagen DNeasy Plant Kit. Isolation using this kit provided the best DNA quality and the greatest legibility of electropherograms. However, it is the most time- and cost-intensive method of DNA isolation, which limits its applicability at a large number of tested genotypes. In terms of electrophoretic image quality good DNA isolation was provided by the method using a Genomic Mini AX Plant Kit. A rapid and cheap method was that proposed by Thomson and Henry; unfortunately, it was less efficient than the former ones. The greatest variation in the amounts of obtained DNA was observed for isolation using the Maxwell kit.
Źródło:
Aparatura Badawcza i Dydaktyczna; 2015, 20, 2; 90-109
2392-1765
Pojawia się w:
Aparatura Badawcza i Dydaktyczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie markerów DNA w selekcji marchwi z cechą cytoplazmatycznej męskiej sterylności typu płatkowego
The use of DNA markers in the selection of carrot lines with cytoplasmic male sterility trait
Autorzy:
Szczechura, W.
Nowakowska, M.
Nowak, K.
Kamiński, P.
Nowak, R.
Kozik, E.U.
Powiązania:
https://bibliotekanauki.pl/articles/2119577.pdf
Data publikacji:
2019
Wydawca:
Instytut Ogrodnictwa
Tematy:
CMS
carrot
DNA markers
marker-assisted selection (MAS)
Opis:
Cytoplasmic male sterility (CMS) is a maternally inherited trait in which a plant is unable to produce functional pollen. It occurs in many plants, including carrots. The expression of this trait depends on environmental conditions, especially temperature – therefore, DNA markers become an excellent tool for selecting carrot genotypes with this feature. In this study, we used seventeen DNA markers available in the literature, linked with various mitochondrial DNA sequences that are associated with the CMS trait. Their amplification was tested on male sterile and male fertile plants. Only five markers – A321/392, I214/401/435, K751, N795, O790 – correctly identified the tested genotypes. They can be used as selection tools in carrot breeding programs.
Źródło:
Zeszyty Naukowe Instytutu Ogrodnictwa; 2019, 27; 53-62
2300-5882
2391-8969
Pojawia się w:
Zeszyty Naukowe Instytutu Ogrodnictwa
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Weryfikacja pochodzenia drzewiastych form kosodrzewiny na terenie Tatrzańskiego Parku Narodowego na podstawie polimorfizmu miejsc insercji transpozonów
Origin assessment of woody mountain pine forms in the Tatra National Park based on transposon insertional polymorphism
Autorzy:
Polok, K.
Zwijacz-Kozica, T.
Zieliński, R.
Powiązania:
https://bibliotekanauki.pl/articles/989538.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Tatrzanski Park Narodowy
sosna gorska
Pinus mugo
sosna drzewokosa
Pinus x rhaetica
pochodzenie roslin
identyfikacja
metody badan
insercja transpozonow
pinus×rhaetica
dna markers
ssap
genetic similarity
Opis:
Closely related Pinus species, mountain pine (Pinus mugo Turra) and Scots pine (Pinus sylvestris L.), belong to native woody species in the Tatra National Park (TPN, southern Poland). Their occurrence in close proximity can lead to the formation of natural hybrids known as Pinus × rhaetica, which is a woody, often polycormic form. Pinus×rhaetica is described in the TPN, but there has been a great deal of disagreement over its origin. The goal of the studies was to verify the taxonomic status of individuals identified as Pinus×rhaetica that grew in the eight stands together with P. mugo and P. sylvestris by SSAP (Sequence Specific Amplification Polymorphism) analysis of transposon insertional polymorphism. In total, 34 Pinus×rhaetica, 25 P. mugo and 27 P. sylvestris individuals were tested in addition to 20 individuals of P. uliginosa from ‘Torfowisko pod Węglińcem' and ‘Wielkie Torfowisko Batorowskie' as well as 25 individuals of P. uncinata from the Austrian Alps as the control groups. Four transposon sequences were employed: a DNA transposon from the CACTA family, Tpo and retrotransposons – two gypsy (Ogre, IFG7) and one copia like (Bare). All species belonging to the Pinus mugo complex are highly variable with 49−81% polymorphic loci and genetic diversity, HTequals 0.228−0.307 with the highest values in Pinus×rhaetica. Surprisingly, P. sylvestris proves to be the least variable species, likely because of a narrow gene pool in small, scattered stands in the Tatras. Very low Nei's genetic similarities between P. sylvestris and Pinus mugo complex, especially in comparison with P. uliginosa (I=0.548) and P. mugo (I=0.558) exclude unequivocally the possibility of spontaneous hybridization among these taxa. Thus, it undermines the hypothesis about hybrid origin of Pinus×rhaetica in the Tatras. It proves to be a morphological form of P. mugo as assessed from the Nei's coefficient, I=0.985 which is well within a range of conspecific populations. Finally, none of the studied individuals of Pinus×rhaetica are derived from seeds of Alpine P. uncinata.
Źródło:
Sylwan; 2016, 160, 07; 573-581
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ działalności antropogenicznej na obecną strukturę genetyczną populacji drzew leśnych
Antropogenic influence on the present genetic structure of forest tree populations
Autorzy:
Nowakowska, Justyna Anna
Powiązania:
https://bibliotekanauki.pl/articles/470370.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Kardynała Stefana Wyszyńskiego w Warszawie
Tematy:
drzewa leśne
markery DNA
inżynieria genetyczna
gospodarka leśna
forest trees
DNA markers
genetic engineering
forest management
Opis:
Human activity has always had an impact on the species composition and genetic structure of species that make up the forest ecosystem. The results of DNA-based studies confirm the influence of current and past forest management on the current level of genetic variability of forest tree populations. Molecular analysis enables the study of gene flow between generations and reflects the history of post-glacial migration of species from southern refugia of Europe to Poland. Thanks to DNA markers, it is possible to precisely distinguish species hybrids, e.g. between Japanese and European larches. In timber trade market, the comparison of the DNA profiles of a stolen tree with a stump left in the forest provides strong evidence in judiciary pursuits concerning the illegal logging. Genetic transformation of the tree genome allows to obtain wood with altered lignin structure, higher increment and increased assimilation of CO2 in the leaves. Genetic modifications are also aimed at enhanced immunity of cultured tree species to fungal pathogens or insect pests and to plant protection products on fast-growing GMO trees.
Działalność człowieka od zawsze miała wpływ na skład gatunkowy i strukturę genetyczną gatunków tworzących ekosystem leśny. Wyniki badań DNA potwierdzają wpływ obecnej i przeszłej gospodarki leśnej na obecny poziom zmienności genetycznej populacji drzew leśnych. Analiza molekularna pozwala określić pokrewieństwo genetyczne gatunków drzew leśnych, umożliwia badanie przepływu genów między pokoleniami, oraz odzwierciedla historię polodowcowej migracji gatunków z refugiów południowej Europy do Polski. Dzięki markerom DNA można precyzyjnie odróżnić mieszańce gatunkowe np. modrzewia japońskiego i europejskiego. Porównanie profili DNA kradzionego drzewa z pniakiem pozostawionym w lesie, dostarcza mocnych dowodów w sprawach dotyczących nielegalnego handlu drewnem. Genetyczna transformacja genomu drzew pozwala uzyskać drewno o zmienionej strukturze lignin, oraz zwiększoną asymilację CO2 w liściach. Modyfikacje genetyczne zmierzają też w kierunku wzmocnionej odporności hodowanych gatunków drzew na patogeny grzybowe lub szkodniki owadzie oraz na środki ochrony roślin na plantacjach drzew szybkorosnących GMO.
Źródło:
Studia Ecologiae et Bioethicae; 2018, 16, 3; 63-71
1733-1218
Pojawia się w:
Studia Ecologiae et Bioethicae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stan zdrowotny i zróżnicowanie genetyczne buka zwyczajnego w Nadleśnictwie Siewierz na podstawie analiz chloroplastowego DNA
Health condition and genetic differentiation level of beech in the Siewierz Forest District assessed with cpDNA markers
Autorzy:
Nowakowska, J.A.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/1013561.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
zroznicowanie genetyczne
cpDNA zob.DNA chloroplastowy
drzewa zdrowe
podatnosc na choroby
czynniki genetyczne
lesnictwo
Phytophthora
drzewa chore
Nadlesnictwo Siewierz
czynniki chorobotworcze
grzyby
buk zwyczajny
fytoftoroza
stan zdrowotny drzew
DNA chloroplastowy
Fagus sylvatica
drzewa lesne
fagus sylvatica
chloroplast dna markers
heterozygosity level
phythopthora spp.
Opis:
Beech decline phenomenon has been observed in Poland since the 1980s. The reasons are still unclear but the discovery of pathogens genus Phytophthora shed a new light on it. Stems as well as roots of beech trees were severely affected by P. citricola and P. cambivora. In order to find out the genetic background of infection, three loci of chloroplast microsatellite DNA were investigated in the genetic differentiation study of beeches growing in the Siewierz Forest District in Poland. Parameters of genetic diversity (h Nei) and differentiation (GST) in the chloroplast genome were estimated and compared between healthy and damaged trees. Healthy beeches were more heterozygous (h=0.243) than the damaged ones (h=0.113), and the distribution of cpDNA alleles was different between these two groups. Healthy trees were nearly 50% more genetically diversed than declining ones.
Źródło:
Sylwan; 2008, 152, 09; 11-20
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmiany w strukturze genetycznej naturalnego odnowienia dębu (Quercus petraea [Matt.] Liebl.) w odniesieniu do drzew matecznych
Changes in genetic structure of sessile oak (Quercus petraea [Matt.] Liebl.) natural regeneration in relation to maternal trees
Autorzy:
Nowakowska, J.A.
Michalska, A.
Zachara, T.
Powiązania:
https://bibliotekanauki.pl/articles/990023.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
drzewa lesne
badania genetyczne
dab bezszypulkowy
Quercus petraea
odnowienia naturalne
struktura genetyczna
markery DNA
drzewa potomne
drzewa mlode
zroznicowanie genetyczne
drzewa mateczne
zmiennosc genetyczna
ssr markers
genetic differentiation
natural regeneration
Opis:
The genetic variability of sessile oak (Quercus petraea L.) mature stand and its natural progeny was investigated. Comparison between genetic structure of parental and progeny trees was based on frequencies of nuclear microsatellite (SSR) alleles occurring in three DNA loci. A slight (4%) increase of gene pool between oak mature and progeny trees was revealed by heterozygosity level estimation, maintaining 86.3% of genetic similarity between generations. Also allele richness, partition probability of basic clustering and inbreed coefficient proved the high genetic similarity between parental and progeny of investigated oak trees. The gene flow occurred within the stands as far as rare alleles were transmitted or new ones appeared in the progenies. The results highlight the necessity of such a study for silvicultural measures taken in order to proceed natural or artificial regeneration in forest tree stand.
Źródło:
Sylwan; 2014, 158, 02; 83-89
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hybridity of a plant created in a combination of crossing of (Vaccinium uliginosum L. × V. vitis-idaea L.) × Oxycoccus macrocarpus (Aiton) Pursh at the tetraploid level
Autorzy:
Marozau, Aleh
Baranov, Oleg Yu.
Powiązania:
https://bibliotekanauki.pl/articles/2045745.pdf
Data publikacji:
2018
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
consecutive crossing
DNA markers
Ericaceae
hybridisation
sequencing
tetraploids
Opis:
The aim of the study was to determine the hybridity of the F1 generation of Vaccinium cf. uliginosum × V. vitis-idaea × Oxycoccus macrocarpus created through the consecutive crossing of some common berry species of the family Ericaceae (bog whortleberry, cowberry and marsh cranberry) at the tetraploid level. Certain aspects of phenology and morphometric parameters of vegetative organs of the supposed hybrid and parent plants were analysed using traditional methods of comparative analysis. Molecular genetic assay, including random amplification of polymorphic DNA, simple sequence repeat and sequencing, were also used. Comparison of the phenological and morphometric features of the experimental plant and its parents allows suggesting that in the combination of crossing of (V. uliginosum × V. vitis-idaea) × O. macrocarpus (the cultivar Searles), a three-species hybrid was created. The allelic variants, specific for V. uliginosum, V. vitis-idaea and O. macrocarpus, were detected in the V. cf. uliginosum × V. vitis-idaea × O. macrocarpus genotype. A next-generation sequencing approach is suggested for estimating the share of the genomes of Vaccinium spp. in the formation of the interspecies hybrid.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2018, 60, 4; 281-291
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Charakterystyka genetyczna świerka Schrenka (Picea schrenkiana) w gradiencie wysokościowym i geograficznym gór Tien-Szan w Kirgistanie
Genetic characteristic of Schrenk spruce (Picea schrenkiana) in the altitudal and geographical gradient in Kyrgyz part of Tien-Shan mountains
Autorzy:
Magnuszewski, M.
Nowakowska, J.A.
Zasada, M.
Orozumbekov, A.
Powiązania:
https://bibliotekanauki.pl/articles/991108.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Kirgistan
drzewa lesne
swierk Schrenka
Picea schrenkiana
zmiennosc genetyczna
polimorfizm DNA
markery SSR
zroznicowanie genetyczne
ssr markers
genetic differentiation
genetic distance
picea schrenkiana
Opis:
The study showed the genetic structure of nine Schrenk spruce stands, which represented altitude and geographical variants in the Tien−Shan mountains in Kyrgyzstan. Comparison between genetic structure of stands was based on frequencies of nuclear microsatellite (SSR) alleles occurring in three DNA loci. The total genetic differentiation of Schrenk spruce populations was low (FST=0.0651). Eight main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers.
Źródło:
Sylwan; 2014, 158, 05; 361-369
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie zmienności genetycznej pokolenia matecznego i sztucznie wyhodowanego potomstwa sosny zwyczajnej na podstawie analiz DNA
Comparison of the genetic variability of Scots pine trees and their progeny from nursery production based on DNA analyses
Autorzy:
Konecka, A.
Tereba, A.
Bieniek, J.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/985843.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
sosna zwyczajna
Pinus sylvestris
zmiennosc genetyczna
analiza DNA
markery mikrosatelitarne
drzewa mateczne
drzewa potomne
sadzonki z zakrytym systemem korzeniowym
genetic diversity
ssr markers
forest nursery production
pinus sylvestris l.
Opis:
The production of forest tree species in forest nurseries is performed via two main breeding systems: i) the traditional (conventional) way with the seedlings grown in soil, and ii) plants cultivated in the containers. The aim of the study was to assess the level of genetic variability in the populations of the mother stands and the progeny populations of Scots pine cultured with traditional way (in soil) and in containers in two nurseries in Olsztynek (N Poland) and Oleszyce (S Poland) forest districts. Four polymorphic microsatellite markers (SPAG 7.14, SPAC 11.6, SPAC 12.5 and SsrPt_ctg4363) were used to evaluate the genetic variability of the studied populations. The basic hypothesis assumed that higher gene pool characterizes the seedlings grown in the containers comparing to the seedlings grown in the ground. The results confirmed that. Seedlings from containerized breeding had larger gene pool and were more diverse than plants with conventional breeding, both in Olsztynek and Oleszyce. Our study revealed a significant human impact on shaping the pool of forest genetic resources of Polish forests at the early stage of nursery production and showed the need for a broader study on further stages of cultivation of forests.
Źródło:
Sylwan; 2018, 162, 01; 32-40
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of Polymorphic DNA Sequences in the Identfication of Individuals and its Possible Use in Biometric Systems
Autorzy:
Kartasińska, E.
Brągoszewska, A.
Powiązania:
https://bibliotekanauki.pl/articles/308733.pdf
Data publikacji:
2015
Wydawca:
Instytut Łączności - Państwowy Instytut Badawczy
Tematy:
DNA identifcation
DNA matching
genetic markers
genetic profiling
Opis:
The article presents the achievements in DNA forensic science practice, the latest developments as well as future trends. The article concerns also other than forensic DNA applications as well as expectations, concerns and obstacles. DNA identification technology unlike other biometric techniques requires the collection of biological material and the identification is not performed in real time. DNA utilized in most of forensic identification, is present almost in every living cell in the body. What is more, each cell of the same body has the same DNA molecule which means that it is possible to compare the DNA sampled from different sources, for example saliva with blood or semen from the same person. Rapid development and reliability of DNA technology contribute to the fact that the analysis of polymorphic DNA sequences constitutes a very important evidence used in the court. The unique properties of DNA and rapid development of DNA analytical devices allow to claim that DNA may assume a more important position amongst biometric data in the future.
Źródło:
Journal of Telecommunications and Information Technology; 2015, 2; 11-17
1509-4553
1899-8852
Pojawia się w:
Journal of Telecommunications and Information Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zróżnicowanie fenotypowe i zmienność tła genetycznego polskich proweniencji sosny zwyczajnej
Phenotypic differentiation and genetic background variation of Polish provenances of Pinus sylvestris L.
Autorzy:
Hebda, A.
Skrzyszewski, J.
Wachowiak, W.
Powiązania:
https://bibliotekanauki.pl/articles/987201.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
drzewa lesne
sosna zwyczajna
Pinus sylvestris
proweniencje krajowe
zmiennosc fenotypowa
zmiennosc genetyczna
markery mikrosatelitarne
DNA mikrosatelitarny
polimorfizm loci
zmiennosc adaptacyjna
scots pine
phenotypic differentiation
neutral variation
microsatellite markers
environment correlation
local adaptation
Opis:
We looked at the patterns of differentiation at a set of phenotypic traits of Scots pine populations derived from different environmental conditions of the species distribution range in Poland. The growth traits were tested over 47 years at the provenance trial experiment in the Carpathians. The phenotypic differentiation of the populations was compared to diversity estimates at a set of ten nuclear microsatellite markers. Scots pine populations were highly differentiated concerning diameter at breast high, stand volume, stem straightness and crown width according to the prove− nance and climatic zones. At the same time a low genetic diversity for microsatellite markers and no evidence of population structure was found among proveniences. Local populations from the Carpathian Mountains had lower genetic variation and weaker growth compared to lowland provenances. Among the tested populations Scots pine from lowlands were characterized by the great growth potential and good adaptation to the severe climate of the Carpathians. Populations that showed high quantitative traits differentiation were genetically homogenous at the neutral loci. The results indicate that there are some regions in the genome under selection that drive species adaptive variation.
Źródło:
Sylwan; 2017, 161, 04; 277-286
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
Autorzy:
Gutkowska, J.
Borys, M.
Tereba, A.
Tkaczyk, M.
Oszako, T.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/1293265.pdf
Data publikacji:
2017
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
lesnictwo
RDLP Krosno
drzewostany swierkowe
stan zdrowotny lasu
drzewa lesne
swierk pospolity
Picea abies
zroznicowanie genetyczne
markery molekularne
markery mikrosatelitarne
DNA mitochondrialny
Norway spruce
health state
SSR markers
mtDNA
genetic diversity
Opis:
The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
Źródło:
Leśne Prace Badawcze; 2017, 78, 1
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pochodzenie jodły pospolitej (Abies alba Mill.) z Nadleśnictwa Osusznica w świetle badań DNA cytoplazmatycznego - ostoja jodły sudeckiej na Pomorzu?
Origin of silver fir (Abies alba Mill.) from Osusznica Forest District revealed by cytoplasmic DNA markers - refugium of Sudety fir in Pomerania?
Autorzy:
Dzialuk, A.
Czarnecki, J.
Gout, R.
Filipiak, M.
Powiązania:
https://bibliotekanauki.pl/articles/993081.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
Nadlesnictwo Osusznica
drzewa lesne
zmiennosc genetyczna
jodla pospolita
Abies alba
pochodzenie roslin
markery genetyczne
DNA chloroplastowy
DNA mitochondrialny
origin of silver fir
abies alba
mt dna
cp dna
genetic markers
Opis:
Genetic markers in mitochondrial and chloroplast DNA were analysed to determine the native status and possibly trace the origin of the silver fir (Abies alba Mill.) growing outside this species natural range in Osusznica Forest District (northern Poland). The provenance of the extralimital populations were determined through comparison with reference samples from the native range of silver fir in Poland and Ukraine. Our results suggest that the Sudety Mts. are the most probable region of the origin of fir from Osusznica. It also seems that the plus seed stands in Osusznica can be treated as a valuable archive of the genetic resources of the fir from the Sudety and, if necessary, may support program of this species restitution.
Źródło:
Sylwan; 2013, 157, 02; 139-148
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
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