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Wyszukujesz frazę "cord blood" wg kryterium: Temat


Wyświetlanie 1-6 z 6
Tytuł:
Anti-Anisakis antibodies in human umbilical cord blood
Przeciwciała anty-Anisakis w ludzkiej krwi pępowinowej
Autorzy:
Figueiredo Jr, Israel
Vericimo, Mauricio Afonso
São Clemente, Sergio Carmona
Brasil Teixeira, Gerlinde Platais
Powiązania:
https://bibliotekanauki.pl/articles/1030876.pdf
Data publikacji:
2018
Wydawca:
Medical Communications
Tematy:
Anisakis spp.
ELISA
cord blood
fish
newborn
Opis:
Introduction: Little is known about the influence of seroreactivity to fish nematode antigens on the unborn child. Aim: The objective of this study was to ascertain whether infants born to women with Anisakis spp. seroreactivity had problems with growth at birth, or during neonatal care. Methods: We conducted a retrospective database search of puerperal women interviewed at two perinatal facilities in Niterói, Brazil. Neonates were selected by the presence of anti-Anisakis IgG antibodies in cord blood and categorised, by analysis of maternal blood results, as reactive or nonreactive. The Mann–Whitney U test was used for hypothesis testing in continuous variables. A generalised linear model was used for binary logistic regression. Results: Of the 147 neonates studied, 121 were labelled as nonreactive and 26, as reactive. There were no significant betweengroup differences in maternal age (p = 0.193), number of prenatal visits (p = 0.362), presence of prenatal conditions (p = 0.980), mode of delivery (p = 0.193), gestational age (p = 0.266), birth weight (p = 0.294), need for resuscitation (p = 0.675), development of conditions during hospital stay (p = 0.201), or length of hospital stay (p = 0.528). There was also no significant association between IgG positivity in cord blood in raw fish intake (p = 1.000) or intake >2 times/week (p = 0.729). Conclusions: The presence of anti-Anisakis IgG in cord blood of infants born to women seroreactive to Anisakis did not pose a hazard to this neonatal population and did not influence growth, conditions at birth, or development of any clinical conditions before hospital discharge.
Wstęp: Niewiele wiemy na temat wpływu seroreaktywności w kierunku antygenów nicieni pasożytujących na rybach na płód. Cel pracy: Niniejsze badanie miało na celu stwierdzenie, czy seroreaktywność matki w kierunku Anisakis spp. wpływa na rozwój dziecka w trakcie ciąży lub bezpośrednio po urodzeniu. Metody: W powyższym celu przeprowadzono retrospektywne badanie analizujące dane pacjentek objętych opieką dwóch placówek położniczych w Niterói w Brazylii. Uwzględnione w badaniu noworodki zostały wyłonione na podstawie obecności przeciwciał IgG anty-Anisakis w krwi pępowinowej i podzielone na dwie grupy, jako charakteryzujące się seroreaktywnością bądź jej brakiem, na podstawie wyników badania krwi matki. W celu sprawdzenia hipotezy dotyczącej zmiennych ciągłych posłużono się testem U Manna–Whitneya. W celu przeprowadzenia binarnej regresji logistycznej zastosowano uogólniony model liniowy. Wyniki: Spośród 147 noworodków objętych badaniem 121 dzieci zaklasyfikowano jako niereaktywne, a 26 – jako reaktywne w kierunku anisakiozy. Nie stwierdzono występowania statystycznie istotnych różnic pomiędzy grupami pod względem wieku matki (p = 0,193), liczby wizyt w okresie ciąży (p = 0,362), występowania u płodu jednostek klinicznych w okresie prenatalnym (p = 0,980), sposobu rozwiązania (p = 0,193), wieku ciąży w momencie rozwiązania (p = 0,266), masy urodzeniowej (p = 0,294), konieczności resuscytacji po urodzeniu (p = 0,675), pojawienia się problemów zdrowotnych u dziecka w trackie pobytu w szpitalu (p = 0,201) ani też długości pobytu w szpitalu (p = 0,528). Nie stwierdzono również statystycznie istotnej korelacji pomiędzy obecnością przeciwciał klasy IgG w krwi pępowinowej a obecnością surowych ryb w diecie matki (p = 1,000) ani też spożyciem surowych ryb częstszym niż dwa razy w tygodniu (p = 0,729). Wnioski: W badanej przez nas populacji noworodków obecność przeciwciał anty-Anisakis klasy IgG w krwi pępowinowej noworodków urodzonych przez matki charakteryzujące się seroreaktywnością w kierunku nicieni z rodziny Anisakis nie stwarzała zagrożenia i nie miała wpływu na rozwój fizyczny płodu, występowanie u niego jednostek chorobowych w momencie urodzenia ani ich pojawienie się w okresie pobytu w szpitalu po urodzeniu.
Źródło:
Pediatria i Medycyna Rodzinna; 2018, 14, 3; 310-313
1734-1531
2451-0742
Pojawia się w:
Pediatria i Medycyna Rodzinna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on free amino acid concentrations in umbilical cord blood plasma of newborns from normoglycaemic and gestational diabetes-complicated pregnancies
Autorzy:
Hawryluk, Jarosław
Grafka, Agnieszka
Gęca, Tomasz
Dzida, Grzegorz
Łopucki, Maciej
Powiązania:
https://bibliotekanauki.pl/articles/972581.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
pregnancy
gestational diabetes mellitus
amino acids
umbilical cord blood
Opis:
Introduction and objective. Gestational diabetes mellitus is a common disease among pregnant women. The aim of the study was to compare plasma concentrations of 24 amino acids in umbilical cord blood in newborns from normoglycaemic pregnancies versus those complicated by gestational diabetes. The relationship between birth weight and length of newborns and placental concentrations of individual amino acids were also assessed. Material and methods. The study comprised 76 pregnant women at the gestational age of 37 weeks and more. The control group consisted of 31 women whose 75-g glucose load test excluded gestational diabetes mellitus. The study group encompassed 45 women with diagnosed gestational diabetes mellitus. The placental plasma concentrations of 24 amino acids were determined using ion-exchange chromatography with an automated amino acid analyser. Results. The concentrations of cysteic acid, aspartic acid, threonine, glutamic acid, cystine, and alpha-aminobutyric acid in umbilical cord blood plasma were higher in the group complicated by gestational diabetes compared to normoglycemic pregnancies; otherwise, concentrations of glutamine, alanine, valine, phenylalanine, lysine and, arginine were lower in the group complicated by gestational diabetes than in the normoglycaemic group. Conclusions. The results show that despite optimal control of carbohydrate metabolism during gestational diabetes mellitus, there are many abnormalities in the amino acids metabolism. This suggests that research on the effects of amino acids concentration in umbilical cord blood on the foetal development in gestational diabetes-complicated pregnancies should be continued.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2016, 10, 1; 12-22
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimisation of transfection conditions of CD34+ hematopoietic cells derived from human umbilical cord blood
Autorzy:
Ołdak, Tomasz
Kruszewski, Marcin
Machaj, Eugeniusz
Gajkowska, Agnieszka
Pojda, Zygmunt
Powiązania:
https://bibliotekanauki.pl/articles/1043723.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CD34+ cells
umbilical cord blood
green fluorescent protein
transfection
Opis:
Human umbilical cord blood is frequently used as a source of transplantable hematopoietic cells and more recently as a target of gene therapy - a new approach for treatment of various disorders. The aim of our study was optimisation of the transfection conditions of cord blood-derived CD34+ hematopoietic cells. Mononuclear cells fraction was isolated from cord blood samples by density gradient centrifugation. Subsequently, CD34+ hematopoietic cells were separated on immunomagnetic MiniMACS columns. Pure population of CD34+ cells was incubated in a serum free medium supplemented with thrombopoietin, stem cell factor and Flt-3 ligand for 48 h and then transfected with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene. We studied the influence of various pulse settings and DNA concentrations on the transfection efficiency, measured by flow cytometry as the fluorescence of target cells due to the expression of EGFP. The optimal settings were as follows: 4 mm cuvette, 1600 μF, 550 V/cm, and 10 μg of DNA per 500 μl. With these settings we obtained a high transfection frequency (41.2%) without a marked decrease of cell viability. An increase of the pulse capacitance and/or of DNA concentration resulted in a greater electroporation efficiency, but also in a decrease of cell viability. In conclusion, the results described here allow one to recommend electroporation as an efficient method of gene delivery into CD34+ hematopoietic cells derived from human umbilical cord blood.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 625-632
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nonviral transfection of human umbilical cord blood dendritic cells is feasible, but the yield of dendritic cells with transgene expression limits the application of this method in cancer immunotherapy
Autorzy:
Markowicz, Sergiusz
Niedzielska, Joanna
Kruszewski, Marcin
Ołdak, Tomasz
Gajkowska, Agnieszka
Machaj, Eugeniusz
Skurzak, Henryk
Pojda, Zygmunt
Powiązania:
https://bibliotekanauki.pl/articles/1041291.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CD34+ cells
umbilical cord blood
green fluorescent protein
electroporation
gene transfer
dendritic cells
Opis:
Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein antigens for immunotherapy purposes. Cord blood mononuclear cells were cultured in serum-free medium in the presence of granulocyte-monocyte colony stimulating factor (GM-CSF), stem cell factor (SCF) and Flt-3 ligand (FL), to generate DC from their precursors, and thereafter transfected by electroporation. Maturation of DC was induced by stimulation with GM-CSF, SCF, FL and phorbol myristate acetate (PMA). Transfected DC strongly expressed EGFP, but transfection efficiency of DC, defined as HLA-DR+ cells lacking lineage-specific markers, did not exceed 2.5%. Expression of the reporter gene was also demonstrated in the DC generated from transfected, purified CD34+ cord blood cells, by stimulation with GM-CSF, SCF, FL, and tumor necrosis factor α (TNF-α). Transfection of CD34+ cells was very efficient, but proliferation of the transfected cells was much reduced as compared to the untransfected cells. Therefore, the yield of transgene-expressing DC was relatively low. In conclusion, nonviral transfection of cord blood DC proved feasible, but considering the requirements for immunotherapy in cancer patients, transfection of differentiated DC or generation of DC from transfected hematopoietic stem cells provide only a limited number of DC expressing the transgene.
Źródło:
Acta Biochimica Polonica; 2006, 53, 1; 203-212
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of lipids, lipoproteins and apolipoproteins concentrations in cord blood serum of newborns from rural and urban environments
Autorzy:
Pac-Kozuchowska, E
Powiązania:
https://bibliotekanauki.pl/articles/51130.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
cord blood serum
lipoprotein
risk factor
lipid
concentration
life style
rural environment
newborn
apolipoprotein
urban environment
Opis:
The purpose of the present study was to check the infl uence of rural and urban environments on some of the parameters of lipids metabolism in the cord blood serum in healthy newborns, as well as the evaluation of the concentration of lipids, lipoproteins and apolipoproteins in the cord blood serum with relation to risk factors of atherosclerosis in the family of the studied newborns. The study included 75 newborns (37 from rural areas and 38 from urban areas). Newborns weight and length were recorded at birth. On the basis of the family history taken from the mothers, the atherosclerosis risk factors were established in the families of the studied newborns. In all of the studied newborns, concentration of triglycerides, total cholesterol and LDL, VLDL and HDL cholesterol as well as of apolipoproteins (apo-AI, apo-B) in the cord blood serum sampled soon after birth were performed. No statistically signifi cant differences between the mean levels of triglycerides, total cholesterol and cholesterol of fractions: LDL, VLDL, HDL and apolipoproteins (apo-AI, apo-B) in the cord blood serum in the newborns from rural and urban areas were found. However, when analysing the concentration of parameters of lipid metabolism in cord blood serum in newborns with regard to gender, higher concentrations of total cholesterol, LDL cholesterol and apo-AI in female newborns from rural areas, and higher HDL cholesterol and apo-AI in female newborns from urban regions were confi rmed. When analysing the concentration of lipids and lipoproteins in cord blood serum in newborns from families with risk factors confi rmed as compared to the families without that risk, both in the rural and urban regions no signifi cant differences were confi rmed. The studies have not proved any signifi cant differences between the levels of lipids, lipoproteins and apolipoproteins in the cord blood serum in newborns from rural and urban areas.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 1
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Źródła komórek macierzystych krwiotworzenia dla potrzeb transplantacji
Sources of hematopoietic stem cells for transplantation
Autorzy:
Rzepecki, Piotr
Gawroński, Krzysztof
Młot, Beata
Oborska, Sylwia
Pielichowski, Wojciech
Waśko-Grabowska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1031036.pdf
Data publikacji:
2011
Wydawca:
Medical Communications
Tematy:
bone marrow
growth factors
hematopoietic stem cells transplantation
mobilization of hematopoietic stem cells
peripheral blood
umbilical cord blood
czynniki wzrostu
krew obwodowa
krew pępowinowa
mobilizacja komórek macierzystych krwiotworzenia
przeszczepianie krwiotwórczych komórek macierzystych
szpik kostny
Opis:
Bone marrow transplantation dates back to the ‘50s, but greatest progress in this therapeutic modality applied successfully in the treatment of several conditions took place mostly during the past 25 years. Among all organ transplantations, bone marrow transplants are second only to kidney transplants. In Poland this therapeutic technique also undergoes a rapid development. During the past 10 years, absolute numbers of various forms of transplantation increased 100-fold, starting from 6 procedures in 1989 to over 700 at present. Essentially, the term bone marrow transplantation (BMT), present in names of international associations and registers, is in fact a colloquialism (e.g. European Group for Blood and Marrow Transplantation). A much more appropriate term would be hematopoietic cell transplantation (HCT). This is because progenitor cells for transplantation may be obtained not only from bone marrow, but also from peripheral blood and umbilical cord blood. The term hematopoietic cell transplantation has a much broader meaning and includes: classic transplantation of bone marrow obtained at surgery, transplantation of peripheral blood stem cells (PBSCT) and umbilical cord blood-derived stem cells. All hematopoietic stem cells express on their surface the CD34+ antigen and glycosylated transmembrane protein, a member of the family of adhesion molecules. In healthy persons, expression of this antigen in bone marrow cells is at the level of 1-3%, while in peripheral blood – 0.01-0.1%, and in umbilical cord blood – 0.1-0.4%. The first source (transplantation material) of hematopoietic progenitor cells was bone marrow, while transplantation of stem cells obtained from peripheral blood and umbilical cord blood started much later (during the ‘80s of the past century).
Źródło:
Current Gynecologic Oncology; 2011, 9, 3; 186-198
2451-0750
Pojawia się w:
Current Gynecologic Oncology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-6 z 6

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