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    Wyświetlanie 1-3 z 3
    Tytuł:
    Viral infections in goose flocks in Poland
    Autorzy:
    Kozdrun, W.
    Wozniakowski, G.
    Samorek-Salamonowicz, E.
    Czekaj, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/31821.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    viral infection
    goose
    flock
    Polska
    parvovirus
    circovirus
    animal disease
    Opis:
    The aim of this study was to determine the infectious agents isolated from infection - suspected geese sent for the diagnostic examination to National Veterinary Research Institute. The birds were sent from goose flocks localized in different parts of Poland. Totally, 1,013 birds from 122 flocks were examined. The presence of goose parvovirus (GPV), goose haemorrhagic polyomavirus (GHPV), and goose circovirus (GoCV) was detected by triplex PCR. The presence of GPV DNA was shown in 36 flocks. The disease was most frequently diagnosed in goslings aging 3.5 weeks (ten flocks), and 2.5 weeks (six flocks). The analysis of the nucleotide sequence of VP1 encoding region has shown close similarity of Polish GPV strains within the group which ranged from 92% to 100%. Moreover, the similarity level of these strains with GPV isolated in Europe was from 91.3% to 100%. The occurrence of GoCV DNA was shown in 25 goose flocks. The presence of GoCV DNA was found among geese aged from 2 to 6 weeks, but predominantly in those aging 3.5 (three flocks) and 5 weeks (five flocks). The sequence analysis of PCR products from the sequenced region of ORFC1 capsid protein of GoCV has shown that Polish isolates share from 85% to 91% similarity with the sequences of GoCV strains isolated in other countries. The presence of DNA of GHPV was found in 3-week-old geese. During the last 2 years the presence of GHPV was confirmed in three flocks of goslings at the age from 3 to 3.5 weeks. During the last 12 years the occurrence of co-infection with GPV and GoCV was detected in six flocks aging from 5 to 6 weeks.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
    Autorzy:
    Jin, Q.Y.
    Feng, L.L.
    Wang, Y.B.
    Li, P.
    Yang, J.F.
    Teng, M.
    Chai, S.J.
    Xing, G.X.
    Zhang, G.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539073.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    porcine circovirus type 2 (PCV2)
    screening of MAbs
    paper test
    Opis:
    A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of long-term vaccination of a breeding herd of pigs against PCV2 on reproductive parameters
    Autorzy:
    Pejsak, Z.
    Kusior, G.
    Pomorska-Mol, M.
    Podgorska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/32499.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    long-term vaccination
    vaccination
    breeding herd
    animal breeding
    pig
    PCV2 virus
    reproductive parameter
    porcine circoviral disease
    circovirus
    sow
    animal reproduction
    reproductive cycle
    Opis:
    The objective of the study was to evaluate an efficacy of sows vaccination protocols in the herd with serious problems affecting efficacy of reproduction. The study was performed in a large pig herd with about 1200 sows. Before vaccination against PCV2, farrowing rate in this farm was about 65%. Sows, boar and replacement gilts were immunized using Circovac vaccine (Merial, France) according to producer’s recommendations. Parameters of production were analyzed since 2007 until 2010 in selected batches of sows inseminated at the same weeks of the year (17th, 18th, 19th and 20th) to eliminate seasonal variability. In total, 940 sows were subjected to the study. No significant changes in management during these years were introduced. The applied protocol of sow herd long-term vaccination proved to be very efficient. All measured production parameters: reproduction rate, number of piglets born alive, birth weight of piglets and number of piglets weaned per a litter improved after implementation of immunization program. Moreover, further improvement was observed with vaccination in the following reproduction cycles. The most spectacular effect of vaccination regarded average farrowing rate that increased from 64.76% in control group to 86.93% after basic vaccination. Two years after implementation of vaccination program this parameter reached 93.6%. Number of piglets weaned per sow per a litter improved from 10.31 to 11.74 after one year of vaccination and remained relatively stable through the following year. Simultaneously, the percentage of newborn piglets with birth weight < 1 kg decreased significantly (p < 0.05). To summarize, vaccination against PCV2 influenced positively the insemination rate, number of piglets born alive and weaned per litter as well as birth body weight and percentage of piglets weighing < 1 kg.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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