Temat: choroby pasożytnicze - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Zwiekszenie swoistosci laboratoryjnej diagnostyki toksokarozy
Autorzy:: Zarnowska-Prymek, H
Powiązania:: https://bibliotekanauki.pl/articles/837216.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: toksokaroza
choroby pasozytnicze
parazytologia
diagnostyka laboratoryjna
Opis:: The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concem: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (≤30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction.
Źródło:: Annals of Parasitology; 2001, 47, 3; 489-496
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Zwiększenie swoistości laboratoryjnej diagnostyki toksokarozy
ENHANCEMENT OF LABORATORY DIAGNOSIS SPECIFICITY IN HUMAN TOXOCAROSIS
Autorzy:: Żarnowska-Prymek, H.
Powiązania:: https://bibliotekanauki.pl/articles/2148050.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: toksokaroza
choroby pasozytnicze
parazytologia
diagnostyka laboratoryjna
Opis:: The aim of the study was to enhance specificity of immunological diagnosis in toxocarosis. The investigations concem: detection of circulating parasite antigens using dot-ELISA technique and detection of specific IgG antigodies using ELISA test with low molecular weight (≤30 kD) excretory-secretory (ES) larval T. canis antigens. The circulating antigens were investigated in 60 sera and 20 anterior chamber fluids from children with ocular form of toxocarosis. These antigens were present only in 3 sera and 2 anterior chamber fluids in children with active intraocular inflamation and fluid antibodies titers 1:5120 and 1:20000. Comparative study with different ELISA tests indicated that use of low molecular weight antigens increase specificity of reaction.
Źródło:: Wiadomości Parazytologiczne; 2001, 47, 3; 489-496
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Znaczenie kliniczne oznaczania awidnosci swoistych przeciwcial IgG w bablowicy watroby wywolanej przez Echinoccocus granulosus
Autorzy:: Stefaniak, J
Kacprzak, E.
Paul, M.
Powiązania:: https://bibliotekanauki.pl/articles/840892.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: immunoglobulina G
choroby pasozytnicze
przeciwciala swoiste
parazytologia lekarska
badania kliniczne
pasozyty
Echinococcus granulosus
poziom awidnosci
bablowica watroby
Opis:: Role of E. granulosus-specific IgG antibody avidity in evaluation of a natural history of the parasite cysts in the liver, duration of infection and immunogenic cysts activity was studied. Thirty-one patients with liver cysts suspected of echinococcosis were examined; E. Granulosus infection was finally confirmed by a fine needle aspiration biopsy or surgery in 29 cases (93.5%) and two non-parasitic simple cysts in the liver were diagnosed. E. granulosus-specific IgG antibody was found in 21 patients (67.7%). High IgG avidity values were observed in 14 patients with liver cysts in a degcneration stage confirmed by imaging techniques. Low IgG avidity values were mostly detected in children with hypoechogenic echinococcal cysts being in a recent stage of infection. Determination of anti-E. Granulosus immunoglobulin G antibody avidity is a very usefull diagnostic tool for a determination of parasitic cysts activity and their developmental age.
Źródło:: Annals of Parasitology; 2001, 47, 3; 477-482
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Znaczenie kliniczne oznaczania awidności swoistych przeciwciał IgG w bąblowicy wątroby wywołanej przez Echinoccocus granulosus
Autorzy:: Stefaniak, J.
Kacprzak, E.
Paul, M.
Powiązania:: https://bibliotekanauki.pl/articles/2148038.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: immunoglobulina G
choroby pasozytnicze
przeciwciala swoiste
parazytologia lekarska
badania kliniczne
pasozyty
Echinococcus granulosus
poziom awidnosci
bablowica watroby
Opis:: Role of E. granulosus-specific IgG antibody avidity in evaluation of a natural history of the parasite cysts in the liver, duration of infection and immunogenic cysts activity was studied. Thirty-one patients with liver cysts suspected of echinococcosis were examined; E. Granulosus infection was finally confirmed by a fine needle aspiration biopsy or surgery in 29 cases (93.5%) and two non-parasitic simple cysts in the liver were diagnosed. E. granulosus-specific IgG antibody was found in 21 patients (67.7%). High IgG avidity values were observed in 14 patients with liver cysts in a degcneration stage confirmed by imaging techniques. Low IgG avidity values were mostly detected in children with hypoechogenic echinococcal cysts being in a recent stage of infection. Determination of anti-E. Granulosus immunoglobulin G antibody avidity is a very usefull diagnostic tool for a determination of parasitic cysts activity and their developmental age.
Źródło:: Wiadomości Parazytologiczne; 2001, 47, 3; 477-482
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Zmiany poziomu dehydrogenazy mleczanowej, gammaglutamylotranspeptydazy, glukozy i azotu alfa-aminowego w surowicy świnek morskich w przebiegu doświadczalnej glistnicy
Changes of the level of lactate dehydrogenase, gamma-glutamylotranspeptidase, glucose and alpha amino nitrogen in blood serum of guinea pigs in the course of experimental ascariasis
Autorzy:: Biaduń, W.
Powiązania:: https://bibliotekanauki.pl/articles/2152541.pdf
Data publikacji:: 1989
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia
choroby pasozytnicze
glistnica
zwierzeta doswiadczalne
swinki morskie
inwazja eksperymentalna
przebieg choroby
surowica krwi
dehydrogenaza mleczanowa
gamma-glutamylotranspeptydaza
glukoza
azot alfa-aminowy
zmiany stezenia
Opis:: The studies were carried out on 90 male guinea pigs ot which 75 were infected per os with a dose of ca. 5000 invasive eggs of Ascaris lumbricoides suis. At the 1st, 2nd, 4th, 7th and 20th day ot intection blood was taken from experimental and control animals. The activity of LDH and its liver traction (spectrophotometric method), GGTP (circle test method) was determined. The level of glucose (orthotoluidin methhod) and alpha amino nitrogen (ninhydrin method was determined. In the course of larval ascariasis an increase in the values of the studied parameters was noted, especially at the 4th and 7th day of infection. The changes in the activity of the studied enzymes evidence disturbances of the structure and function of the liver of infected animals. The increased level of glucose and alpha amino nitrogen is probably associated with a disturbance of basic energy metabolism of the host organism.
Źródło:: Wiadomości Parazytologiczne; 1989, 35, 4; 305-314
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Zmiany immunologiczne u kobiet z rzęsistkowicą narządów moczowo-płciowych i u ich partnerów seksualnych
Immunologic changes in women with urogenital trichomoniasis and in their sexual partners
Autorzy:: Cvetkova, A.
Andreeva, N.
Hristova, O.
Grigorov, L.
Kuncheva, A.
Powiązania:: https://bibliotekanauki.pl/articles/2152600.pdf
Data publikacji:: 1988
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia lekarska
choroby czlowieka
choroby ukladu moczowo-plciowego
choroby pasozytnicze
rzesistkowica
zmiany immunologiczne
kobiety
partnerzy seksualni
Opis:: The authors studied immunological changes in 44 women with chronic trichomonadal colpitis and in 36 men with symptomless infection of urethra (age 19-50 years). In all the patients all other diseases were excluded. The presence of T. vaginalis in the vagina content and the secretion of the urethra was detected by means of microscope examination of fresh slides and of culture on Trimed medium. Besides, in all the patients complete morphological picture of blood as well as erythrocyte sedimentation rate (Biernacki reaction) were examined in order to find a relationship between these parameters. In the studies on the cellular immunological response the following tests were made: a) estimate of the number of early and late erythrocyte rosettes, and the absolute number of T lymphocytes in peripheral blood, by means of the test of rosette formation (it was assumed, after Bach, that a lymphocyte with five or more erythrocytes (of sheep) around it is a rosette), b) estimate of the absolute number of erythrocyte and B lymphocyte rosettes in peripheral blood, by means of the zymosan test of rosette formation; c) skin test of the delayed type with diluted tuberculine (1 : 5000), read after 24 hrs basing on the occurrence of erythema and induration. The results were statistically analysed using methods of comparative analysis. They were compared with the results of the tests on a control group comprising 40 healthy persons (20 men and 20 women) at age of 27-58 years. The results are presented in the table. The results of the test of erythrocyte sedimentation rate, not included in the table, were within the normal range in all the examined patients. Basing on the presented results the authors are of opinion that in the trichomoniasts immunological reactions of humoral and cellular type occur, both types being detectable almost to the same extent.
Źródło:: Wiadomości Parazytologiczne; 1988, 34, 2; 191-196
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Zmiany immunologiczne u kobiet z rzęsistkowicą narządów moczowo-płciowych i u ich partnerów seksualnych
Immunologic changes in women with urogenital trichomoniasis and in their sexual partners
Autorzy:: Cvetkova, A.
Andreeva, N.
Hristova, O.
Grigorov, L.
Kuncheva, A.
Powiązania:: https://bibliotekanauki.pl/articles/836250.pdf
Data publikacji:: 1988
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia lekarska
choroby czlowieka
choroby ukladu moczowo-plciowego
choroby pasozytnicze
rzesistkowica
zmiany immunologiczne
kobiety
partnerzy seksualni
Opis:: The authors studied immunological changes in 44 women with chronic trichomonadal colpitis and in 36 men with symptomless infection of urethra (age 19-50 years). In all the patients all other diseases were excluded. The presence of T. vaginalis in the vagina content and the secretion of the urethra was detected by means of microscope examination of fresh slides and of culture on Trimed medium. Besides, in all the patients complete morphological picture of blood as well as erythrocyte sedimentation rate (Biernacki reaction) were examined in order to find a relationship between these parameters. In the studies on the cellular immunological response the following tests were made: a) estimate of the number of early and late erythrocyte rosettes, and the absolute number of T lymphocytes in peripheral blood, by means of the test of rosette formation (it was assumed, after Bach, that a lymphocyte with five or more erythrocytes (of sheep) around it is a rosette), b) estimate of the absolute number of erythrocyte and B lymphocyte rosettes in peripheral blood, by means of the zymosan test of rosette formation; c) skin test of the delayed type with diluted tuberculine (1 : 5000), read after 24 hrs basing on the occurrence of erythema and induration. The results were statistically analysed using methods of comparative analysis. They were compared with the results of the tests on a control group comprising 40 healthy persons (20 men and 20 women) at age of 27-58 years. The results are presented in the table. The results of the test of erythrocyte sedimentation rate, not included in the table, were within the normal range in all the examined patients. Basing on the presented results the authors are of opinion that in the trichomoniasts immunological reactions of humoral and cellular type occur, both types being detectable almost to the same extent.
Źródło:: Annals of Parasitology; 1988, 34, 2
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Zjadliwość szczepów Trichomonas vaginalis w zależności od postaci klinicznej rzęsistkowicy
Virulence of Trichomonas vaginalis strains according to clinical form of trichomonosis
Autorzy:: Malyszko, E.
Januszko, T.
Smorczewska-Czuprynska, B.
Ustymowicz-Farbiszewska, J.
Powiązania:: https://bibliotekanauki.pl/articles/839181.pdf
Data publikacji:: 1991
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia
choroby pasozytnicze
pasozyty
rzesistek pochwowy
Trichomonas vaginalis
szczepy chorobotworcze
zjadliwosc
rzesistkowica
postac kliniczna
Opis:: Estimation of virulence of T. vaginalis strains injected subcutaneously to mice was done. T. vaginalis strains were obtained from the urinogenital tracts of patients with different clinical forms of trichomonosis. The virulence of T. vaginalis strains measured by infiltration size was found to depend upon a clinical form of trichomonosis. It was moreover shown that the cutaneous infiltration size is proportional to a dose of Trichomonas administered subcutaneously. Histopathological analysis of cutaneous infiltration itself is not decisive as to the strains virulence, yet in addition to macroscopic examination it may be a criterium in the studies on strain pathogenicity.
Źródło:: Annals of Parasitology; 1991, 37, 2
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Zjadliwość szczepów Trichomonas vaginalis w zależności od postaci klinicznej rzęsistkowicy
Virulence of Trichomonas vaginalis strains according to clinical form of trichomonosis
Autorzy:: Małyszko, E.
Januszko, T.
Smorczewska-Czupryńska, B.
Ustymowicz-Farbiszewska, J.
Powiązania:: https://bibliotekanauki.pl/articles/2152362.pdf
Data publikacji:: 1991
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia
choroby pasozytnicze
pasozyty
rzesistek pochwowy
Trichomonas vaginalis
szczepy chorobotworcze
zjadliwosc
rzesistkowica
postac kliniczna
Opis:: Estimation of virulence of T. vaginalis strains injected subcutaneously to mice was done. T. vaginalis strains were obtained from the urinogenital tracts of patients with different clinical forms of trichomonosis. The virulence of T. vaginalis strains measured by infiltration size was found to depend upon a clinical form of trichomonosis. It was moreover shown that the cutaneous infiltration size is proportional to a dose of Trichomonas administered subcutaneously. Histopathological analysis of cutaneous infiltration itself is not decisive as to the strains virulence, yet in addition to macroscopic examination it may be a criterium in the studies on strain pathogenicity.
Źródło:: Wiadomości Parazytologiczne; 1991, 37, 2; 219-223
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Zimnica - Plasmodium falciparum - trudny problem diagnostyczny i terapeutyczny
Malaria (Plasmodium falciparum) - a difficult diagnostic and therapeutic problem
Autorzy:: Kociecka, W.
Skoryna, B.
Kurczewska, M.
Myjak, P.
Powiązania:: https://bibliotekanauki.pl/articles/837429.pdf
Data publikacji:: 1988
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia lekarska
choroby czlowieka
choroby pasozytnicze
malaria
czynniki chorobotworcze
zarodziec sierpowaty
Plasmodium falciparum
diagnostyka
terapeutyka
Opis:: The authors deal with the problem of malaria induced by Plasmodium falciparum and imported to Poland by people returning from tropics. They stress significance of the variable clinical pattern involved and of chloroquine resistance. Basing on their own observations of a definitely diagnosed malaria (22 out 35 cases observed and suspected of malaria) the authors discuss diagnostic and therapeutic difficulties encountered in 7 patients with Plasmodium falciparum-induced malaria. Two representative cases of malaria (Plasmodium falciparum) have been discussed in detail. One of the cases with malaria imported from tropics, presented severe course and an atypical clinical pattern (with involvement of central nervous system) which made appropriate diagnosis difficult and delayed application of specific causal treatment. The other unusual case involved a nurse who contracted Plasmodium through a small skin wound with which patient's blood came into contact when the nurse was drawing blood for testing. Course of the disease was grave, with deep anaemia and central nervous system and kidney involvement. In both cases the disease had favourable outcome due to complex anti-malarial therapy and multispecific medical intervention.
Źródło:: Annals of Parasitology; 1988, 34, 4-6
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Zimnica - Plasmodium falciparum - trudny problem diagnostyczny i terapeutyczny
Malaria (Plasmodium falciparum) - a difficult diagnostic and therapeutic problem
Autorzy:: Kocięcka, W.
Skoryna, B.
Kurczewska, M.
Myjak, P.
Powiązania:: https://bibliotekanauki.pl/articles/2152680.pdf
Data publikacji:: 1988
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parazytologia lekarska
choroby czlowieka
choroby pasozytnicze
malaria
czynniki chorobotworcze
zarodziec sierpowaty
Plasmodium falciparum
diagnostyka
terapeutyka
Opis:: The authors deal with the problem of malaria induced by Plasmodium falciparum and imported to Poland by people returning from tropics. They stress significance of the variable clinical pattern involved and of chloroquine resistance. Basing on their own observations of a definitely diagnosed malaria (22 out 35 cases observed and suspected of malaria) the authors discuss diagnostic and therapeutic difficulties encountered in 7 patients with Plasmodium falciparum-induced malaria. Two representative cases of malaria (Plasmodium falciparum) have been discussed in detail. One of the cases with malaria imported from tropics, presented severe course and an atypical clinical pattern (with involvement of central nervous system) which made appropriate diagnosis difficult and delayed application of specific causal treatment. The other unusual case involved a nurse who contracted Plasmodium through a small skin wound with which patient's blood came into contact when the nurse was drawing blood for testing. Course of the disease was grave, with deep anaemia and central nervous system and kidney involvement. In both cases the disease had favourable outcome due to complex anti-malarial therapy and multispecific medical intervention.
Źródło:: Wiadomości Parazytologiczne; 1988, 34, 4-6; 649-653
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Zastosowanie metody ISAGA w wykrywaniu swoistych przeciwcial IgM, IgA, IgE w nabytej i wrodzonej toksoplazmozie
Autorzy:: Paul, M
Powiązania:: https://bibliotekanauki.pl/articles/839324.pdf
Data publikacji:: 1997
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: toksoplazmoza
choroby pasozytnicze
przeciwciala swoiste
metody serologiczne
immunoglobulina M
parazytologia
immunoglobulina A
immunoglobulina E
metoda ISAGA
wykrywanie
Toxoplasma gondii
pasozyty czlowieka
Opis:: Immunocapture assays ISAGA PLUS IgA/IgM (bioMerieux) and IgE ISAGA were used to determine their usefulness in the diagnosis of acquired and congenital toxoplasmosis. Specific IgM, IgA and IgE antibodies were tested in 134 patients, namely pregnant women who seroconverted during gestation (n= 20), children with congenital toxoplasmosis (n= 5), patients with toxoplasmic lymphadenitis (n= 56) and immunocompetent individuals with chronic Toxoplasma gondii infection (n= 53). Altogether 172 sera were examined. Specific IgM antibodies were detected in all sera from pregnant women (100%) with recent T. gondii infection (1- 8 weeks after seroconversion), in all patients with toxoplasmic lymphadenopathy (1-3 months after onset of symptoms) and in their control examinations after 2 and 5 months (100%) and also in 35 (66%) out of 53 patients with chronic infection. In infants with congenital toxoplasmosis IgM were found only in one new-born; equivocal results were obtained in 3 children during the asymptomatic serological reactivation in the second year of life. Specific IgA antibodies were present in sera from 15 (75%) out of 20 women seroconverted during pregnancy; in 3 cases the results were equivocal. IgA antibodies were detected in sera from 30 (81.1%) out of 37 patients with toxoplasmic lymphadenitis examined once; in 19 patients examined 3 times IgA antibodies were present in all the cases in the first serological examination performed when clinical symptoms were first observed (100%), in 17 patients after 2 months (89.5%) and in 11 patients after 5 months (57.9%). IgA antibodies were also detected in 21 sera (39.6%) from patients with chronic T. gondii infection. In children with congenital toxoplasmosis IgA antibodies were found in 3 cases during serological reactivation after discontinuation of pyrimethamine-sulfadiazine therapy; in these cases equivocal results of IgM antibodies were present, and positive result of IgE antibodies in one case. Specific IgE antibodies were detected in sera from 17 (85%) out of 20 women with seroconversion and in 18 patients with lymphadenopathy (32.1%); in the last group IgE antibodies were not present in the follow-up examination after 5 months. IgE antibodies were detected only in 5 cases (9.4%) with chronic infection. IgA and IgE antibodies in ISAGA begin to appear about a week later than IgM antibodies; in sera collected between the 2nd and 3rd week after invasion the positive results were obtained in all cases (100%). Therefore, ISAGA PLUS IgA/IgM (bioMerieux) is useful for the diagnosis of recent T. gondii infection especially in women with suspected seroconversion during pregnancy. ISAGA PLUS IgA/IgM is more sensitive than any conventional method routinely used and so far is a specially eflicient technique for newborns and infants suspected for congenital infection and/or in diagnosing congenital toxoplasmosis during immunological recrudescence. This test has a limited value in toxoplasmosis with lymphadenopathy by reason of possibility of a long persistence of IgM and IgA antibodies detected by ISAGA. Detection of specific IgE antibodies using ISAGA technique may be useful for differential diagnosis of acute and chronic phase of T. gondii infection and also in some cases of serological reactivation of congenital toxoplasmosis.
Źródło:: Annals of Parasitology; 1997, 43, 1; 39-51
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Zastosowanie metody ISAGA w wykrywaniu swoistych przeciwciał IgM, IgA, IgE w nabytej i wrodzonej toksoplazmozie
IMMUNOSORBENT AGGLUTINATION ASSAY (ISAGA) IN DETECTION OF SPECIFIC IgM, IgA, AND IgE ANTIBODIES IN ACQUIRED AND CONGENITAL TOXOPLASMOSIS
Autorzy:: Paul, M.
Powiązania:: https://bibliotekanauki.pl/articles/2148892.pdf
Data publikacji:: 1997
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: toksoplazmoza
choroby pasozytnicze
przeciwciala swoiste
metody serologiczne
immunoglobulina M
parazytologia
immunoglobulina A
immunoglobulina E
metoda ISAGA
wykrywanie
Toxoplasma gondii
pasozyty czlowieka
Opis:: Immunocapture assays ISAGA PLUS IgA/IgM (bioMerieux) and IgE ISAGA were used to determine their usefulness in the diagnosis of acquired and congenital toxoplasmosis. Specific IgM, IgA and IgE antibodies were tested in 134 patients, namely pregnant women who seroconverted during gestation (n= 20), children with congenital toxoplasmosis (n= 5), patients with toxoplasmic lymphadenitis (n= 56) and immunocompetent individuals with chronic Toxoplasma gondii infection (n= 53). Altogether 172 sera were examined. Specific IgM antibodies were detected in all sera from pregnant women (100%) with recent T. gondii infection (1- 8 weeks after seroconversion), in all patients with toxoplasmic lymphadenopathy (1-3 months after onset of symptoms) and in their control examinations after 2 and 5 months (100%) and also in 35 (66%) out of 53 patients with chronic infection. In infants with congenital toxoplasmosis IgM were found only in one new-born; equivocal results were obtained in 3 children during the asymptomatic serological reactivation in the second year of life. Specific IgA antibodies were present in sera from 15 (75%) out of 20 women seroconverted during pregnancy; in 3 cases the results were equivocal. IgA antibodies were detected in sera from 30 (81.1%) out of 37 patients with toxoplasmic lymphadenitis examined once; in 19 patients examined 3 times IgA antibodies were present in all the cases in the first serological examination performed when clinical symptoms were first observed (100%), in 17 patients after 2 months (89.5%) and in 11 patients after 5 months (57.9%). IgA antibodies were also detected in 21 sera (39.6%) from patients with chronic T. gondii infection. In children with congenital toxoplasmosis IgA antibodies were found in 3 cases during serological reactivation after discontinuation of pyrimethamine-sulfadiazine therapy; in these cases equivocal results of IgM antibodies were present, and positive result of IgE antibodies in one case. Specific IgE antibodies were detected in sera from 17 (85%) out of 20 women with seroconversion and in 18 patients with lymphadenopathy (32.1%); in the last group IgE antibodies were not present in the follow-up examination after 5 months. IgE antibodies were detected only in 5 cases (9.4%) with chronic infection. IgA and IgE antibodies in ISAGA begin to appear about a week later than IgM antibodies; in sera collected between the 2nd and 3rd week after invasion the positive results were obtained in all cases (100%). Therefore, ISAGA PLUS IgA/IgM (bioMerieux) is useful for the diagnosis of recent T. gondii infection especially in women with suspected seroconversion during pregnancy. ISAGA PLUS IgA/IgM is more sensitive than any conventional method routinely used and so far is a specially eflicient technique for newborns and infants suspected for congenital infection and/or in diagnosing congenital toxoplasmosis during immunological recrudescence. This test has a limited value in toxoplasmosis with lymphadenopathy by reason of possibility of a long persistence of IgM and IgA antibodies detected by ISAGA. Detection of specific IgE antibodies using ISAGA technique may be useful for differential diagnosis of acute and chronic phase of T. gondii infection and also in some cases of serological reactivation of congenital toxoplasmosis.
Źródło:: Wiadomości Parazytologiczne; 1997, 43, 1; 39-51
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Zastosowanie awidnosci swoistych przeciwcial IgG dla roznicowania aktywnego i przewleklego okresu zarazenia Toxocara canis
Autorzy:: Rychlicki, W.
Powiązania:: https://bibliotekanauki.pl/articles/837130.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: Toxocara canis
toksokaroza
pomiary awidnosci
choroby pasozytnicze
przeciwciala swoiste
parazytologia
przeciwciala IgG
diagnostyka
infekcja pasozytnicza
Źródło:: Annals of Parasitology; 2004, 50, 2; 229-236
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Tytuł:: Zastosowanie awidności swoistych przeciwciał IgG dla różnicowania aktywnego i przewlekłego okresu zarażenia Toxocara canis
Use of specific immunoglobulin G antibody avidity in the differential diagnosis of active and chronic Toxocara canis infections.
Autorzy:: Rychlicki, W.
Powiązania:: https://bibliotekanauki.pl/articles/2146457.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: Toxocara canis
toksokaroza
pomiary awidnosci
choroby pasozytnicze
przeciwciala swoiste
parazytologia
przeciwciala IgG
diagnostyka
infekcja pasozytnicza
Opis:: To differentiate between acute and past Toxocara canis infections, a modified enzyme-linked immunosorbent assay, based on the dissociation of antigen-antibody complex with 6 M urea solution was used to measure levels of avidity of specific immunoglobulin G antibody against Toxocara canis excretory-secretory antigen. IgG avidity index was determined in the sera of 212 patients, divided into two groups with various stages of Toxocara infection. Patients were classified according to time period, from the onset of clinical symptoms or first positive serology result. Low IgG avidity index was found in the sera of 6.6% of patients in the acute phase, and all samples from the chronic stage of infection were characterized by high avidity values above 0.5 (mean 0.74). It was documented, that the avidity test for specific IgG antibodies is a valuable diagnostic method that helps to distinguish the early from the later phase of Toxocara canis infection. Indications for anti-parasitic treatment according to immunological activity of Toxocara infection were discussed.
Źródło:: Wiadomości Parazytologiczne; 2004, 50, 2; 229-236
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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