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    Wyświetlanie 1-3 z 3
    Tytuł:
    Separation of Cells From Plasma by Means of Ultrasonics
    Autorzy:
    Włoch, Andrzej
    Czyż, Henryka
    Jasiński, Tadeusz
    Powiązania:
    https://bibliotekanauki.pl/articles/177025.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    ultrasound standing wave
    medical diagnostics
    body fluids
    blood
    red blood cells
    cells separation
    plasma
    Opis:
    This paper presents an analysis of use of ultrasonic standing wave in cell separation from bodily fluids based on the example of erythrocyte separation from plasma. It describes movement of red blood cells in plasma under the influence of the acoustic field (whose forces result from interaction of red blood cells with plasma as the vibrating medium) and under the influence of resistance forces in Stokes’ and Oseen’s approximation. The general properties of solutions of the motion equation are given. The solutions for the parameters of the ultrasonic wave and blood cells which are interesting in terms of practical applications in medical diagnostics are discussed. Time constants of the cell transportation to the regions of stable equilibrium in the field of ultrasonic standing wave are estimated. The formulas which determine the time needed to obtain the assumed concentration increase in plasma in nodes and/or anti-nodes of the standing wave are derived.
    Źródło:
    Archives of Acoustics; 2019, 44, 2; 357-363
    0137-5075
    Pojawia się w:
    Archives of Acoustics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Aspects of Applicability King - St. Clair Approximation in a Non-Newtonian Fluid Mechanics
    Autorzy:
    Włoch, A.
    Czyż, H.
    Jasiński, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/1032505.pdf
    Data publikacji:
    2017-07
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    acoustic radiation force
    acoustic standing wave
    medical applications of ultrasound
    separation of red blood cells from human blood
    Opis:
    This studyis a contribution to research on the biomedical and clinical applications of ultrasound. Our research concerns the procedure for the separation of human blood fractions - erythrocytes. Ultrasonic waves can be used for the separation of cells in human blood. From a physical point of view, the human blood is a suspension of liquids and solids (cell elements), and behaves like a non-Newtonian fluid. Our work is devoted to the problem of the motion of red cells in human blood under the influence of ultrasonic wave. It defines a range of the applicability of approximation consisting in neglecting the nonlinear term in the friction force. It also analyzes the general properties of the equation of motion of the cell in the case of large attenuation constants, corresponding to the values of the drift forces for the cells with radii of a few μm. Finally, it defines the applicability criterion of the so-called King-St Clair approximation consisting in the assumption of equilibrium between the drift and the Stokes viscosity forces, neglecting the term representing inertia. This approximation permits analytical estimation of the time constants for the cell transport to points of stable equilibrium in an ultrasonic standing wave field.
    Źródło:
    Acta Physica Polonica A; 2017, 132, 1; 164-166
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Surface functionalization of poly(L-lactide-co-glycolide) membranes with amphiphilic poly(2-oxazoline) for guided tissue regeneration and treatment of bone tissue defects
    Autorzy:
    Tryba, A. M.
    Krok-Borkowicz, M.
    Paluszkiewicz, C.
    Pamuła, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/283890.pdf
    Data publikacji:
    2018
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    poly (L-lactide-co-glycolide)
    poly (ethylene glycol)
    poly (2-oxazolines)
    phase separation
    Fourier transform infrared spectroscopy (FTIR)
    osteoblast-like cells
    guided tissue regeneration (GTR)
    bone tissue engineering
    Opis:
    The main challenge of this research was to functionalize the surface of poly(L-lactide-co-glycolide) (PLGA) membranes with amphiphilic poly(2-oxazoline) (POx) in order to change PLGA chemical state and properties. Poly(2-oxazolines) are very powerful polymers, which thanks to active pendant groups can be easily functionalized with biologically active molecules or peptides. The membranes were prepared by dissolving PLGA, POx, and poly(ethylene glycol) (PEG, 1000 Da) in methylene chloride (DCM), followed by PEG leaching. POx molecules were preferentially adsorbed at the interface PLGA-POx-PEG thanks to affinity to both hydrophilic (PEG) and hydrophobic (PLGA) chains. The properties of the membranes were characterized with Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and wettability tests. Cytocompatibility of the materials in contact with osteoblast-like MG-63 cells was studied by evaluation of cell viability (Alamar-Blue test), live/dead and phalloidin/DAPI staining. The results show that the presence of POx influenced topography of the PLGA membranes, but did not have an impact on their wettability. All membranes were fo-und cytocompatible with model osteoblasts. Presence of POx resulted in better cell adhesion as shown by microscopic studies after fluorescence staining for nuclei and cytoskeleton actin filaments. In summary, one-step phase separation process between PLGA, PEG, and POx, dissolved in DCM followed by drying and PEG leaching resulted in cytocompatible PLGA membranes with immobilised POx, which might be considered for guided tissue regeneration technique in periodontology and in bone tissue engineering.
    Źródło:
    Engineering of Biomaterials; 2018, 21, 147; 16-20
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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