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    Tytuł:
    Differences of α3β1 integrin glycans from different human bladder cell lines.
    Autorzy:
    Lityńska, Anna
    Przybyło, Małgorzata
    Książek, Dorota
    Laidler, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1044370.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    integrins
    bladder cell lines
    oligosaccharides
    Opis:
    Expression as well as properties of integrins are altered upon transformation. Cell adhesion regulated by integrins is modulated by glycosylation, one of the most frequent biochemical alteration associated with tumorogenesis. Characterisation of carbohydrate moieties of α3β1 integrin on the cultured human bladder carcinoma (T-24, Hu456, HCV 29T) and human normal ureter and bladder epithelium (HCV 29, Hu609) cell lines was carried out after an electrophoresis and blotting, followed by immunochemical identification of α3 and β1 integrin chains and analysis of their carbohydrates moieties using highly specific digoxigenin-labelled lectins. In all the studied cell lines α3β1 integrin was glycosylated although in general each subunit differently. Basic structures recognized in β1 subunit were tri- or tetraantennary complex type glycans in some cases sialylated (T-24, HCV 29, HCV 29T) and fucosylated (Hu609, HCV 29T). Positive reaction with Phaseolus vulgaris agglutinin and Datura stramonium agglutinin suggesting the presence of β1-6 branched N-linked oligosaccharides was found in cancerous cell lines (T-24, Hu456) as well as in normal bladder epithelium cells (Hu609). High mannose type glycan was found only in β1 subunit from Hu456 transitional cell cancer line. On the other hand α3 subunit was much less glycosylated except the invasive cancer cell line T-24 where high mannose as well as sialylated tri- or tetraantennary complex type glycans were detected. This observation suggests that changes in glycosylation profile attributed to invasive phenotype are rather associated with α3 not β1 subunit.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 2; 427-434
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of differentiation agents on inflammatory and oxidative responses of the human neuroblastoma cell line SK-N-SH
    Autorzy:
    Niewiarowska-Sendo, Anna
    Patrzalek, Katarzyna
    Kozik, Andrzej
    Guevara-Lora, Ibeth
    Powiązania:
    https://bibliotekanauki.pl/articles/1038980.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Neuroblastoma cell lines
    RA
    PMA
    Opis:
    Obtaining a suitable experimental cellular model is a major problem for neuroscience studies. Neuroblastoma cell lines have been often applied in studies related to pathological disorders of nervous system. However, in the search for an ideal model, these cells must be differentiated to cancel their tumor character. The subsequent reactions that are caused by differentiation are not always indifferent to the same model. We evaluated the effect of two well known substances, used for SH-N-SK cell line differentiation, retinoic acid (RA) and phorbol-12-myristate-13-acetate (PMA), on the induction of pro-inflammatory and pro-oxidative reactions in these cells. Cells differentiated with PMA were able to produce significantly higher amounts of pro-inflammatory cytokines whereas the release of nitric oxide radicals was similar to that in undifferentiated cells. On the contrary, in RA-differentiated cells no significant changes in cytokine production were observed and the nitric oxide release was decreased. Additionally, the RA-differentiated neuronal model was more sensible to lipopolysaccharide stimulation, producing pro-inflammatory cytokines abundantly. These results suggest that RA-differentiated SH-N-SK cells provide a more suitable experimental model for the study of molecular and cellular mechanisms of the inflammation and oxidative stress in neuronal cells.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 3; 435-443
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation.
    Autorzy:
    Lityńska, Anna
    Przybyło, Małgorzata
    Pocheć, Ewa
    Laidler, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1043727.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycosylation
    integrins
    bladder cell lines
    adhesion
    Opis:
    Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the α2, a5 and β1 integrin subunits inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the β1 integrin subunit. Antibodies to α3 integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that α3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. N-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 3; 643-650
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of β1-integrins and N-cadherin in bladder cancer and melanoma cell lines.
    Autorzy:
    Laidler, Piotr
    Gil, Dorota
    Pituch-Noworolska, Anna
    Ciołczyk, Dorota
    Książek, Dorota
    Przybyło, Małgorzata
    Lityńska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044269.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cytofluorimetry
    cancer
    integrins
    cadherins
    cell lines
    Opis:
    Changes in the expression of integrins and cadherins might contribute to the progression, invasion and metastasis of transitional cell cancer of the bladder and of melanomas. The expression of α5 (P < 0.001), α2 and β1 (P < 0.05 - P < 0.001) integrin subunits in melanoma cells from noncutaneous metastatic sites (WM9, A375) were significantly increased as compared to cutaneous primary tumor (WM35) and metastatic (WM239) cell lines. These differences might be ascribed to the invasive character of melanoma cells and their metastasis to the noncutaneous locations. The significantly heterogeneous expression of β1 integrin subunit in two malignant bladder cancer cell lines (T24 and Hu456) and nonsignificant differences in the expression of α2, α3, and α5 subunits between malignant and non-malignant human bladder cell lines do not allow an unanimous conclusion on the role of these intergrin subunits in the progression of transitional cancer of bladder. The adhesion molecule, expressed in all studied melanoma and bladder cell lines, that reacted with anti-Pan cadherin monoclonal antibodies was identified as N-cadherin except in the HCV29 non-malignant ureter cell line. However, neither this nor any other bladder or melanoma cell line expressed E-cadherin. The obtained results imply that the replacement of E-cadherin by N-cadherin accompanied by a simultaneous increase in expression of a2, a3 and a5 integrin subunits clearly indicates an increase of invasiveness of melanoma and, to a lesser extent, of transitional cell cancer of bladder. High expression of N-cadherin and a5 integrin subunit seems to be associated with the most invasive melanoma phenotype.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 1159-1170
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Novel potential anticancer agents
    Autorzy:
    Jęśkowiak, Izabela
    Ryng, Stanisław
    Powiązania:
    https://bibliotekanauki.pl/articles/1178224.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    anticancer agents
    carcinoma cell lines
    drug design
    Opis:
    Cancer is a widespread and lethal disease. It is considered as the first leading cause of deaths in economically developed countries. Nonetheless, the search for an effective treatment of cancer is still a major challenge. In this review, we analyzed the core structure of compounds and their impact on antitumor activity. Diversity of heterocyclic ring in the innovatory anticancer substances showed the potential in the design and development of new anticancer drugs.
    Źródło:
    World Scientific News; 2018, 93; 40-49
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Actin in human colon adenocarcinoma cells with different metastatic potential.
    Autorzy:
    Nowak, Dorota
    Krawczenko, Agnieszka
    Duś, Danuta
    Malicka-Błaszkiewicz, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1043684.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    state of actin polymerization
    colon adenocarcinoma cell lines
    actin
    Opis:
    Four human colon adenocarcinoma cell line variants with different metastatic potential were used to examine whether a correlation exists between actin level, state of actin polymerization and invasiveness of tumour cells. Monomeric (G), total (T) and filamentous (F) actin were determined in the cytosolic fraction of these cells. A statistically significant decrease in G actin level and increase in the state of actin polymerization (measured by F:G actin ratio) were found in the cytosol of three cell variants with higher metastatic potential and invasiveness (EB3, 3LNLN, 5W) compared with the parental cell line (LS180). Our experimental data lead to the conclusion that there is a correlation between the metastatic capacity of human colon adenocarcinoma cells and the state of actin polymerization.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 823-828
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The structural and functional analysis of the human HSPA2 gene promoter region
    Autorzy:
    Pigłowski, Wojciech
    Nowak, Radosława
    Krawczyk, Zdzisław
    Ścieglińska, Dorota
    Powiązania:
    https://bibliotekanauki.pl/articles/1041117.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    expression regulation
    cancer cell lines
    transcript structure
    HSPA2
    Opis:
    HSPA2 is a human counterpart of the testis-specific rodent Hst70/Hsp70.2 gene. In contrast to the latter, the expression of the human HSPA2 gene is not limited to the testis, and recent data show that human tumor cells can express this gene at significant levels. The characteristics of HSPA2 expression suggests that it can influence the phenotype and survival of cancer cells similarly as overexpression of major members of the HSP70 gene family. Until now, neither the structure of the transcription unit of the human HSPA2 gene has been established nor a functional analysis of its promoter performed. In this study we established that the human HSPA2 gene, in contrast to its rodent counterparts, is intronless and has a single transcription start site. We also show that the same type of HSPA2 transcripts are synthesized in the testes and in cancer cell lines. In order to perform a functional study of the HSPA2 promoter, we used a transient transfection assay and found that the 392 bp fragment upstream of the ATG codon was a minimal region required for efficient transcription, while a 150 bp deletion from the 5' end of this region dramatically reduced the promoter activity. Delineation of the minimal promoter is a basic step toward identifiying the cis and trans elements involved in the regulation of the HSPA2 gene expression in cancer cells.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 1; 99-106
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression and hypoxia-responsiveness of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 in mammary gland malignant cell lines
    Autorzy:
    Minchenko, Oleksandr
    Opentanova, Iryna
    Ogura, Tsutomu
    Minchenko, Dmytro
    Komisarenko, Sergiy
    Caro, Jaime
    Esumi, Hiroyasu
    Powiązania:
    https://bibliotekanauki.pl/articles/1041336.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    dimethyloxalylglycine
    hypoxia
    mammary gland cancer cell lines
    PFKFB4
    HIF-1
    Opis:
    Recently, we have shown that PFKFB4 gene which encodes the testis isoenzyme of PFKFB is also expressed in the prostate and hepatoma cancer cell lines. Here we have studied expression and hypoxic regulation of the testis isoenzyme of PFKFB4 in several malignant cell lines from a female organ - the mammary gland. Our studies clearly demonstrated that PFKFB4 mRNA is also expressed in mammary gland malignant cells (MCF-7 and T47D cell lines) in normoxic conditions and that hypoxia strongly induces it expression. To better understand the mechanism of hypoxic regulation of PFKFB4 gene expression, we used dimethyloxalylglycine, a specific inhibitor of HIF-1α hydroxylase enzymes, which strongly increases HIF-1α levels and mimics the effect of hypoxia. It was observed that PFKFB4 expression in the MCF7 and T47D cell lines was highly responsive to dimethyloxalylglycine, suggesting that the hypoxia responsiveness of PFKFB4 gene in these cell lines is regulated by HIF-1 proteins. Moreover, desferrioxamine and cobalt chloride, which mimic the effect of hypoxia by chelating or substituting for iron, had a similar stimulatory effect on the expression of PFKFB mRNA. In other mammary gland malignant cell lines (BT549, MDA-MB-468, and SKBR-3) hypoxia and hypoxia mimics also induced PFKFB4 mRNA, but to variable degrees. The hypoxic induction of PFKFB4 mRNA was equivalent to the expression of PFKFB3, Glut1, and VEGF, which are known HIF-1-dependent genes. Hypoxia and dimethyloxalylglycine increased the PFKFB4 protein levels in all cell lines studied except MDA-MB-468. Through site-specific mutagenesis in the 5'-flanking region of PFKFB4 gene the hypoxia response could be limited. Thus, this study provides evidence that PFKFB4 gene is also expressed in mammary gland cancer cells and strongly responds to hypoxia via an HIF-1α dependent mechanism. Moreover, the PFKFB4 and PFKFB3 gene expression in mammary gland cancer cells has also a significant role in the Warburg effect which is found in all malignant cells.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 881-888
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of ROS by a novel chromone linked nitrone derivative promotes mitochondria mediated caspase dependent apoptosis in HepG2 and HeLa cells
    Autorzy:
    Mandal, Supratim
    Mallick, Suvadip
    Maiti, Sourav
    Bandyopadhya, Chandrakanta
    Pal, Chiranjib
    Powiązania:
    https://bibliotekanauki.pl/articles/1177437.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Anticancer activity
    Apoptosis
    Caspases
    Chromones
    HeLa-HepG2 cell lines
    Mitochondrial membrane potential
    Reactive oxygen species generation
    Opis:
    Chromones are organic compounds reported to induce cytotoxic effect in an extensive variety of cells. Consequently, the synthesis and reorientation of the chromone molecules are of great interest for many researchers because of their miscellaneous biological activities. The present study was designed to assess the significant antitumor effects of C-(6-Methyl-4-oxo-4H-1-benzopyran-3-yl)-N-(p-tolyl) nitrone, a novel chromone linked nitrone derivative and to elucidate the mechanism of these effects on two human cancer cell lines HepG2 and HeLa. Cell proliferation was analysed by the MTT assay. Apoptosis was evaluated by DAPI staining and flow cytometric analysis and quantified by fluorometric assays for Caspase 3 and 9. Apaf-1 and cytochrome c expression were identified by means of Western Blot analysis. The derivative showed significant dose dependent cytotoxic effects in the cancer cells and induced the reactive oxygen species and endogenous nitric oxide production. Furthermore, mitochondrial membrane potential depolarization, translocation of mitochondrial cytochrome c to cytosol, induction of Apaf-1 and activation of caspases were observed during the derivative-mediated apoptosis. These findings proposed that the novel chromone linked nitrone derivative has significant antitumor effects on HepG2 and HeLa cells and have immense scope to develop as an anticancer agent.
    Źródło:
    World Scientific News; 2018, 103; 167-185
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines
    Autorzy:
    Guvensen, N.C.
    Keskin, D.
    Gunes, H.
    Oktay, M.K.
    Yildirim, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2083066.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Centaura babylonica
    antimicrobial activity
    antiproliferative activity
    A-549
    PC-3
    MCF-7 and HeLa cell lines
    chemical
    composition
    GC-MS
    Opis:
    Introduction and objective. Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and leaves of C. babylonica (L.) L. were prepared to determine antimicrobial and antiproliferative activity against cancer cell lines. Materials and method. MIC assay was used for antimicrobial activity against gram positive and gram negative bacteria, and one yeast. MTT assay was applied to screen the antiproliferative activity of seven extracts, and to determine dose- and time-dependent effects of the aceton extract on A549, PC-3, MCF-7, and HeLa cell lines. Results. The aceton extract of C.babylonica (L.) L. showed the best antibacterial activity against Bacillus cereus, P. aeruginosa and C. albicans (MIC: 1.6 mg/mL). GC-MS analyses allowed six compounds to be determined; the main constituents of acetone extract from C. babylonica (L.) L. were diacetone alcohol (53.47 %), 1-dexadecene (10.19 %) and 1-tetradecene (8.67 %). In addition, seven different solvent extracts at 500 μg/mL caused antiproliferative activity between 84% – 88%, compared to control. Dose-dependent effects of the extracts on A549 cells indicated that chloroform, ethyl acetate, and aceton extract were the most effective extracts with the IC50 values of 9, 33, and 36 μg/mL, respectively. Conclusions. The results clearly demonstrate that C. babylonica (L.) L. exhibited a strong antimicrobial effect and antiproliferative activity against cancer cells in vitro. Further studies are required to isolate and characterize the active pure compounds responsible for the antimicrobial and antiproliferative activities.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 2; 290-297
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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