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Wyświetlanie 1-15 z 27
Tytuł:
Transcriptional pattern of TGF-beta1 inhibitory effect on mouse C2C12 myoblasts differentiation
Autorzy:
Wicik, Z.
Sadkowski, T.
Jank, M.
Motyl, T.
Powiązania:
https://bibliotekanauki.pl/articles/30377.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
transforming growth factor-beta 1
myogenesis
microarray
DNA microarray
differentiation
myoblast
mouse
mice
muscle cell
myosin heavy chain
Opis:
The aim of the present study was to define the effect of TGF-β1 on C2C12 myoblasts myogenesis. TGF-β1 together with its receptor is a negative auto-paracrine regulator of myogenesis, which influences the proliferation, differentiation, and functions of muscle cells. TGF-β1 exerts highly significant inhibitory effect on differentiation of C2C12 mouse myoblasts manifested by the impairment of cell fusion and very low expression of myosin heavy chain. The study of differentiating C2C12 mouse myoblasts treated with TGF-β1 revealed 502 genes (436 down-regulated and 66 up-regulated) with statistically different expression. TGF-β1-regulated genes were identified to be involved in 29 biological processes, 29 molecular functions groups and 59 pathways. The strongest inhibiting effect of TGF-β1 was observed in the cadherin and Wnt pathways. The key-genes that could play the role of TGF-β1 targets during myoblasts differentiation was identified such as: Max, Creb1, Ccna2, Bax, MdfI, Tef, Tubg1, Cxcl5, Rho, Calca and Lgals4.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermodynamics of irreversible plant cell growth
Autorzy:
Pietruszka, M
Lewicka, S.
Pazurkiewicz-Kocot, K.
Powiązania:
https://bibliotekanauki.pl/articles/58662.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
thermodynamics
irreversible process
plant cell
plant growth
cell wall
yielding
growth stimulator
growth inhibitor
modified growth equation
Opis:
The time-irreversible cell enlargement of plant cells at a constant temperature results from two independent physical processes, e.g. water absorption and cell wall yielding. In such a model cell growth starts with reduction in wall stress because of irreversible extension of the wall. The water absorption and physical expansion are spontaneous consequences of this initial modification of the cell wall (the juvenile cell vacuolate, takes up water and expands). In this model the irreversible aspect of growth arises from the extension of the cell wall. Such theory expressed quantitatively by time-dependent growth equation was elaborated by Lockhart in the 60's.The growth equation omit however a very important factor, namely the environmental temperature at which the plant cells grow. In this paper we put forward a simple phenomenological model which introduces into the growth equation the notion of temperature. Moreover, we introduce into the modified growth equation the possible influence of external growth stimulator or inhibitor (phytohormones or abiotic factors). In the presence of such external perturbations two possible theoretical solutions have been found: the linear reaction to the application of growth hormones/abiotic factors and the non-linear one. Both solutions reflect and predict two different experimental conditions, respectively (growth at constant or increasing concentration of stimulator/inhibitor). The non-linear solution reflects a common situation interesting from an environmental pollution point of view e.g. the influence of increasing (with time) concentration of toxins on plant growth. Having obtained temperature modified growth equations we can draw further qualitative and, especially, quantitative conclusions about the mechanical properties of the cell wall itself. This also concerns a new and interesting result obtained in our model: We have calculated the magnitude of the cell wall yielding coefficient (T) [m3 J-1•s-1] in function of temperature which has acquired reasonable numerical value throughout.
Źródło:
Acta Societatis Botanicorum Poloniae; 2006, 75, 3; 183-190
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Therapeutic potential of heme oxygenase-1 in cardiovascular disease
Autorzy:
Jazwa, A.
Florczyk, U.
Stepniewski, J.
Jozkowicz, A.
Dulak, J.
Powiązania:
https://bibliotekanauki.pl/articles/80176.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
angiogenesis
angiogenic factor
antiapoptotic effect
antiinflammatory effect
antioxidant effect
carbon monoxide
cardiovascular disease
cytoprotection
endothelial cell
ferrous iron
heme oxygenase-1
hypoxia
iron
stem cell
stromal cell
therapeutic potential
vascular endothelial growth factor-A
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The two-phase Hell-Shaw flow: construction of an exact solution
Autorzy:
Malaikah, K. R.
Powiązania:
https://bibliotekanauki.pl/articles/265877.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
falka Laplace'a
twierdzenie Schwarza
Laplace growth
Hele-Shaw cell
Schwarz function
free boundary problem
Opis:
We consider a two-phase Hele-Shaw cell whether or not the gap thickness is time-dependent. We construct an exact solution in terms of the Schwarz function of the interface for the two-phase Hele-Shaw flow. The derivation is based upon the single-valued complex velocity potential instead of the multiple-valued complex potential. As a result, the construction is applicable to the case of the time-dependent gap. In addition, there is no need to introduce branch cuts in the computational domain. Furthermore, the interface evolution in a two-phase problem is closely linked to its counterpart in a one-phase problem.
Źródło:
International Journal of Applied Mechanics and Engineering; 2013, 18, 1; 249-257
1734-4492
2353-9003
Pojawia się w:
International Journal of Applied Mechanics and Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The shRNA-mediated silencing of VEGF-C illustrates its role in proliferation, chemosensitization, tumour colonization, and anchorage independence
Autorzy:
Tambe, P.
Purohit, I.
Suneja, D.
More, S.
Desai, P.
Shrivastava, N.
Powiązania:
https://bibliotekanauki.pl/articles/80325.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
vascular endothelial growth factor
gene expression
endothelial cell
breast cancer
lymphangiogenesis
metastasis
proliferation
chemosensitivity
tumour cell
RNAi
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of Arabidopsis thaliana aladin-related protein in the regulation of plant growth
Autorzy:
Queval, G.
Foyer, C.
Powiązania:
https://bibliotekanauki.pl/articles/80756.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
root meristem
thioredoxin
tripeptide thiol glutathione
cell cycle
nuclear pore complex
Arabidopsis thaliana
AAAS gene
plant growth
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The presence of CD25 on bovine WC1 plus gamma delta T cells is positively correlated with their production of IL-10 and TGF-beta, but not IFN-gamma
Autorzy:
Maslanka, T.
Jaroszewski, J.J.
Markiewicz, W.
Ziolkowski, H.
Barski, D.
Powiązania:
https://bibliotekanauki.pl/articles/32183.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cattle
WC1 cell
T cell
T lymphocyte
transforming growth factor-beta
interleukin 10
interferon-gamma
CD25 antigen
CD25 molecule
Opis:
WC1+ cells in cattle exhibit both regulatory and effector activities. However, it has not been elucidated whether they are so plastic that both activities co-exist in one cell or there are separate subpopulations of effector and regulatory cells. Since the production of IFN-γ and IL-10 seems to be related to WC1+ cells’ effector and regulatory function, respectively, the main aim of this study was to determine whether those cytokines are produced by separate subpopulations of WC1+, or are co-produced by the same cells. Due to increasingly frequent emphasised role of consumption of IL-2 in the mechanism of suppressor action of mouse CD25+CD4+ T regulatory cells, expression of the receptor’s α chain for interleukin 2 (CD25) on WC1+ lymphocytes has been evaluated. An average of 5.21% of WC1+ cells obtained from PBMCs of 12-month-old heifers show constitutive expression of the CD25 molecule, with CD25highWC1+ and CD25lowWC1+ cells accounting for 1.05% and 4.10% of WC1+ lymphocytes, respectively. For detection of intracellular cytokine production, PBMCs were stimulated with concanavalin A. Both IFN-γ- and IL-10-producing cells within the CD25-WC1+ and CD25+WC1+ subpopulations were mainly separate subpopulations. The average percentage of IFN-γ+IL-10-, IFN-γ-IL-10+ and IFN-γ+IL-10+ cells among CD25-WC1+ lymphocytes was 4.03%, 2.67% and 0.51%, respectively. A positive correlation was observed between the presence of the CD25 molecule on WC1+ lymphocytes and production of IL-10 and TGF-β, because the average percentage of IFN-γ-IL-10+ and IFN-γ+IL-10+ among CD25+WC1+ lymphocytes was 3 and 4.5 times higher as compared to the corresponding cells in the CD25-WC1+ subpopulation, whereas the percentage of IFN-γ+IL-10- cells in both the subpopulations was not significantly different. The percentage of TGF-β+ cells within the CD25+WC1+ subpopulation was 2.72 times as high as that of CD25-WC1+ lymphocytes. Therefore, with respect to the production of IFN-γ, IL-10 and TGF-β, CD25+WC1+ lymphocytes turn out to have a more suppressor profile than CD25-WC1+.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The pattern of lignin deposition in the cell walls of internodes during barley (Hordeum vulgare L.) development
Autorzy:
Begovic, L.
Ravlic, J.
Lepedus, H.
Leljak-Levanic, D.
Cesar, V.
Powiązania:
https://bibliotekanauki.pl/articles/19860.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant development
barley
Hordeum vulgare
lignin
cell wall
internode
stem
growth stage
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of selective COX-2 inhibitor on phase of healing surgical wounds: proliferation and secretion of bFGF by endothelial cells
Autorzy:
Jasiak, Łukasz
Kowalczyk, Mateusz
Mazan, Paula
Kowalczyk, Edward
Sienkiewicz, Monika
Jóźwiak-Bębnista, Marta
Wiktorowska-Owczarek, Anna
Powiązania:
https://bibliotekanauki.pl/articles/763865.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
Tematy:
angiogenesis, selective COX-2 inhibitor, fibroblast growth factor, vascular endothelial cell
Opis:
The process of wound healing consists of the following phases: inflammation, proliferation, remodeling. Non-steroidal antiinflammatory drugs may be important in this process, especially in a stage called angiogenesis. For this reason, it was decided to investigate the effect of selective COX-2 (cyclooxygenase 2) inhibitor (NS-398) on the proliferation of endothelial cells and their ability to secrete bFGF (fibroblast growth factor) for vascular endothelial cells (HMEC-1). For determination of the secretion of bFGF in a cell line HMEC-1 immunosorbent ELISA assays were used. In turn, the cell proliferation assay was performed using the MTT method. Using MTT method, it was found that NS-398 at 10 μM did not affect cell viability. Whereas selective COX-2 inhibitor at 100 μM decreased cell viability in a statistically significant manner and inhibited the proliferative effect of 100 μg/mL LPS at concentrations of 10 and 100 μM. In the further step, application of NS-398 (10 and 100 μM) with LPS (100 μg/mL; inflammatory environment) reduced the secretion of bFGF in a statistically significant manner. The investigations showed that NS-398 has an antiangiogenic effect which is based on reducing the proliferation of vascular endothelial cells and inhibiting the secretion of bFGF- factor responsible for angiogenesis during wound healing.
Źródło:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2017, 72, 1
2083-3563
0066-2232
Pojawia się w:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The evaluation of the efficacy and toxicity of targeted treatment in non-small cell lung cancer patients – single centre experience
Autorzy:
Kardas, Joanna
Buraczewska, Agnieszka
Chrom, Paweł
Waśko-Grabowska, Anna
Młot, Beata
Szczylik, Cezary
Powiązania:
https://bibliotekanauki.pl/articles/773534.pdf
Data publikacji:
2017
Wydawca:
Medical Education
Tematy:
endothelial growth factor receptor
non-small cell lung cancer
tyrosine kinase inhibitor
Opis:
Introduction: Tyrosine kinase inhibitors (TKI) are the standard of treatment in patients with advanced non-small cell lung cancer (NSCLC) with EGFR (endothelial growth factor receptor) gene activating mutation. Objective: The evaluation of the efficacy and toxicity of TKI drugs in NSCLC patients treated in single centre. Material and methods: NSCLC patients treated with TKI (gefitinib, erlotynib, afatinib) between 2012– 2016 were retrospectively analysed. We evaluated: overall response rate (ORR) which is the sum of complete responses (CR) and partial remissions (PR), progression free survival (PFS), overall survival (OS) and adverse events (AE) according to CTCAE (Common Terminology Criteria for Adverse Events) scale. Results: The study group were 16 patients ORR was 50% (CR: 1, PR: 7). Median PFS and OS was 8,7 and 22,9 months respectively. Adverse events observed mainly in stage 1 and 2 were related to hyponatraemia, hyperbilirubinemia, skin toxicity and mucositis. There was one death reported due to infectious complications. Conclusion: The efficacy and toxicity of TKI in study group were found to be similar to those described in the literature.
Źródło:
OncoReview; 2017, 7, 2; 92-97
2450-6125
Pojawia się w:
OncoReview
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of undecanones and their derivatives on tumor angiogenesis and VEGF content
Autorzy:
Gibka, J
Wasiutynski, A.
Skopinska-Rozewska, E.
Siwicki, A.K.
Chorostowska-Wynimko, J.
Sommer, E.
Mazurkiewicz, M.
Glinski, M.
Skurzak, H.
Wojcik, R.
Jung, L.
Powiązania:
https://bibliotekanauki.pl/articles/31734.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mice
mouse
undecan-x-one
derivative
angiogenesis
tumour
VEGF zob.vascular endothelial growth factor
enantiomer
histological examination
vascular endothelial growth factor
sarcoma cell
Opis:
The in vivo effects of some derivatives of aliphatic ketones (2-undecanone, 3-undecanone, 4-undecanone and their derivatives) on L-l sarcoma tumor angiogenesis and VEGF content were studied in Balb/c mice. Mice that inhaled 10% solution of 3-undecanone(3-on) or 1% solution of 2-undecanone propylene acetal (Acpr2) for 3 days after tumor cells implantation, presented lower neovascular response measured by tumor-induced cutaneous angiogenesis test (TIA) and lower tumor VEGF content in 5-days tumors, than non-inhaled controls. Other substances presented various effects on tumor VEGF concentration and angiogenesis. Histological examination of lesions collected from mice inhaled Acpr2, or non-inhaled controls, revealed small diffused areas of necrosis in the former group. In both groups, slight to moderate inflammatory infiltrations were seen at the tumor's margin. In Acpr2 group, there were less small blood vessels at tumor's margin than in the control group.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 1; 105-115
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The 3D simulation model for growth and cell divisions applied to the surface layer of cells of the shoot apex
Autorzy:
Nakielski, J.
Kucypera, K.
Piekarska-Stachowiak, A.
Lipowczan, M.
Powiązania:
https://bibliotekanauki.pl/articles/80257.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
simulation model
3D model
cell division
growth
superficial layer
cell
statistical analysis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regulation of nuclear phospholipase C activity.
Autorzy:
Manzoli, Lucia
Billi, Anna
Martelli, Alberto
Cocco, Lucio
Powiązania:
https://bibliotekanauki.pl/articles/1043270.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
nucleus
cell growth
regulation
phospholipase C
Opis:
A body of evidence, linking inositide-specific phospholipase C (PI-PLC) to the nucleus, is quite extensive. The main isoform in the nucleus is PI-PLCβ1, whose activity is up-regulated in response to insulin-like growth factor-1 (IGF-1) or insulin stimulation. Whilst at the plasma membrane this PI-PLC is activated and regulated by Gαq/α11 and Gβg subunits, there is yet no evidence that qα/α11 is present within the nuclear compartment, neither GTP-γ-S nor AlF4 can stimulate PI-PLCβ1 activity in isolated nuclei. Here we review the evidence that upon occupancy of type 1 IGF receptor there is translocation to the nucleus of phosphorylated mitogen-activated protein kinase (MAPK) which phosphorylates nuclear PI-PLCβ1 and triggers its signalling, hinting at a separate pathway of regulation depending on the subcellular location of PI-PLCβ1. The difference in the regulation of the activity of PI-PLCβ1mirrors the evidence that nuclear and cytoplasmatic inositides can differ markedly in their signalling capability. Indeed, we do know that agonists which affect nuclear inositol lipid cycle at the nucleus do not stimulate the one at the plasma membrane.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 391-395
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease
Autorzy:
Khurshid, Rukhshan
Saleem, Mahjabeen
Gul-e-Raana, -
Akhthar, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1039198.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation sites
HER2/c-erbB-2
cell growth and disease
Opis:
The protein kinase c-erbB-2 belongs to the family of receptor tyrosine kinase and is involved in oncogenesis. The present study predicts different phosphorylation sites of HER2/c-erbB-2 which are important in preventing or developing cancer, especially breast cancer. Sequence homology showed highest homology (77%) with epidermal growth factor receptor kinase domain. According to PROSITE search result, active sites of c-erbB-2 are N-lobe (glycine rich phosphate binding loop). Catalytic loop with presumptive catalytically active of Asp108 is phosphorylated by tyrosine protein kinase. A-loop, activation loop, becomes phosphorylated and activates the substrate binding. The study strengthens our knowledge regarding HER2 signaling by the detection of uncharacterized signaling proteins, establishing phosphorylation of an activation loop and helps us to make assumptions about the role of such previously unidentified proteins. On the basis of importance of HER2 in breast cancer as well as in other diseases, this study provides fruitful information for designing new therapeutic strategies.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 699-703
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Osteoblasts response to novel chitosan/agarose/hydroxyapatite bone scaffold – studies on MC3T3-E1 and hFOB 1.19 cellular models
Autorzy:
Kazimierczak, Paulina
Vivcharenko, Vladyslav
Truszkiewicz, Wiesław
Wójcik, Michał
Przekora, Agata
Powiązania:
https://bibliotekanauki.pl/articles/284277.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone tissue engineering
biocompatibility
osteoconductivity
cell growth
osteogenic differentiation
Opis:
Since it is known that various cell lines may ex-press different behaviours on the scaffolds surface, a comprehensive analysis using various cellular mo-dels is needed to evaluate the biomedical potential of developed biomaterials under in vitro conditions. Thus, the aim of this work was to fabricate bone scaffolds composed of a chitosan-agarose matrix reinforced with nanohydroxyapatite and compare the biological response of two cell lines, i.e. mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4) and human foetal osteoblasts (hFOB 1.19). Within this study, the osteoblasts number on the scaffold surface and the osteogenic markers level produced by MC3T3-E1 and hFOB 1.19 cells were determined. Furthermore, changes in calcium and phosphorous ions concentrations in the culture media dedicated for MC3T3-E1 and hFOB 1.19 were estimated after the biomaterial incubation. The obtained results proved that the fabricated biomaterial is characterized by biocompatibility and osteoconductivity since it favours osteoblasts attachment and growth. It also supports the production of osteogenic markers (collagen, bALP, osteocalcin) by MC3T3-E1 and hFOB 1.19 cells. Interestingly, the developed biomaterial exhibits different ion reactivity values in the two culture media dedicated for the mentioned cell lines. It was also revealed that mouse and human osteoblasts differ in the cellular response to the fabricated scaffold. Thus, the use of at least two various cellular models is recommended to carry out a reliable biological characterization of the novel biomaterial. These results demonstrate that the tested bone scaffold is a promising biomaterial for bone regeneration applications, however further biological and physicochemical experiments are essential to fully assess its biomedical potential.
Źródło:
Engineering of Biomaterials; 2019, 22, 151; 24-29
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 27

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