- Tytuł:
- Differentiated Paju cells have increased resistance to toxic effects of potassium ionophores.
- Autorzy:
-
Teplova, Vera
Jääskeläinen, Elina
Salkinoja-Salonen, Mirja
Saris, Nils-Erik
Serlachius, Martina
Li, Feng-Yen
Andersson, Leif - Powiązania:
- https://bibliotekanauki.pl/articles/1043288.pdf
- Data publikacji:
- 2004
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
cereulide
Paju cells
valinomycin
mitochondria
cell differentiation
membrane potential - Opis:
- In this study we have investigated the impact of differentiation of neuronal cells on their sensitivity to microbial toxins. We used the human neural crest-derived tumor cell line Paju, which can be induced to differentiation in vitro by treatment with phorbol 12-myristate 13-acetate. Addition of the highly toxic potassium ionophores cereulide (4.5 and 9.0 ng/ml) or valinomycin (20 ng/ml), to cultures of undifferentiated Paju cells caused collapse of the mitochondrial membrane potential - measured with the fluorescent probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetrabenzimidazole carbocyanine iodide (JC-1) followed by detachment of the cells and their apoptotic death. After induced differentiation of the Paju cells, their mitochondria retained the membrane potential upon exposure to the toxins and the cells displayed increased resistance to apoptosis as compared with undifferentiated cells. This effect may be caused by an elevated expression of the anti-apoptotic protein Bcl-2 and of the neuroprotective factor, stanniocalcin, in differentiated cells.
- Źródło:
-
Acta Biochimica Polonica; 2004, 51, 2; 539-544
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł