Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "cell cycle" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 41
Tytuł:
CAMPTOTHECIN INHIBITS MIGRATION, INVASION AND CLONOGENIC PROPERTY OF LIVER CANCER CELLS BY MODULATING MICRORNA EXPRESSION
Autorzy:
Liu, Zhenzhong
Wu, Song
Li, Xiaoqian
Powiązania:
https://bibliotekanauki.pl/articles/895460.pdf
Data publikacji:
2020-04-29
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
Hepatocellular Carcinoma
cell proliferation
cell cycle
Camptothecin
miRNA
Opis:
Camptothecin (CPT), an alkaloid natural product, extracted from Camptotheca acuminata bark, has been reported to have potential antitumor activity in diverse cancers. MicroRNAs (MiRNAs) are a class of short, non-coding RNAs that plays a crucial role in the normal physiology by attenuating translation. Here, we showed that the CPT modulates the expression of miRNAs in hepatocellular carcinoma cells (HCC). Microarray analysis reveals that CPT modulates the expression of as many as 39 miRNAs in HCC cells (Huh7), 27 miRNAs were downregulated whereas 12 miRNAs were upregulated. miR-16 is the key miRNA upregulated by CPT and targets key prosurvival proteins (MMP-2, MMP-9 and cyclin D1). Our results demonstrate that CPT is inhibiting cell viability of HCC cells significantly when compared with the untreated cells. Wound healing and colony formation assay confirm inhibition of cell migration and clonogenic property of Huh7 cells respectively, upon the dose-dependent treatment of CPT. Furthermore, the Boyden chamber assay analysis revealed a significant inhibition of number of invasive cells in CPT treated cells with comparison to untreated Huh7 cells. Mechanistically, CPT upregulates miR-16 expression which targets MMP-2, MMP-9, cyclin D1 downregulation and subsequently upregulates the expression of E-cadherin, TIMP1, p21, and p27, thereby inhibits cell migration, invasion and clonogenic property of HCC cells. In summary, CPT treatment in Huh7 cells decreases cell viability and upregulates miR-16 expression, which results in inhibition of cell migration, invasion and clonogenic property of cells, by decreasing MMP-2, MMP-9, cyclin D1 and increasing the expression of cell cycle-regulated proteins p21 and p27.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 2; 295-304
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On continuous time version of two-phase cell cycle model of Tyrcha
Autorzy:
Zwoleński, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/748777.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Matematyczne
Tematy:
cell cycle
transport equations
invariant density
Markov operators
Opis:
We consider a model of two-phase cell cycle in a maturity-structured cellular population, which consists of a system of first order linear partial differential equations (transport equations). The model is based on similar biological assumptions as models of Lasota-Mackey, Tyson-Hannsgen and Tyrcha. We examine behavior of the solutions of the system along characteristics, give conditions for existence of invariant density, and compare results with outcomes of generational model.
Źródło:
Mathematica Applicanda; 2013, 41, 1
1730-2668
2299-4009
Pojawia się w:
Mathematica Applicanda
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dual effect of 2-methoxyestradiol on cell cycle events in human osteosarcoma 143B cells.
Autorzy:
Gołębiewska, Justyna
Rozwadowski, Piotr
Spodnik, Jan
Knap, Narcyz
Wakabayashi, Takashi
Woźniak, Michał
Powiązania:
https://bibliotekanauki.pl/articles/1043807.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
osteosarcoma 143B cells
2-methoxyestradiol
apoptosis
cell cycle
Opis:
We have demonstrated for the first time that the steroid metabolite, 2-methoxyestradiol (2-ME) is a powerful growth inhibitor of human osteosarcoma 143 B cell line by pleiotropic mechanisms involving cell cycle arrest at two different points and apoptosis. The ability of 2-ME to inhibit cell cycle at the respective points has been found concentration dependent. 1 μM 2-ME inhibited cell cycle at G1 phase while 10 μM 2-ME caused G2/M cell cycle arrest. As a natural estrogen metabolite 2-ME is expected to perturb the stability of microtubules (MT) in vivo analogously to Taxol - the MT binding anticancer agent. Contrary to 2-ME, Taxol induced accumulation of osteosarcoma cells in G2/M phase of cell cycle only. The presented data strongly suggest two different mechanisms of cytotoxic action of 2-ME at the level of a single cell.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 59-65
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyanidin and peonidin inhibit SPCA-1 growth in vitro via inducing Cell Cycle Arrest and Apoptosis
Autorzy:
Yue, Heng
Xu, Qianqian
Lv, Liuzhuang
Xu, Jianfeng
Fan, Hua
Wang, Wenhan
Powiązania:
https://bibliotekanauki.pl/articles/895348.pdf
Data publikacji:
2019-06-28
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
Lung cancer
apoptosis
cell cycle
SPCA-1
cyanidin
peonidin
Opis:
Non-small cell lung cancer (NSCLC) accounts for the majority (85%) of all lung cancers. Although many therapies are available, 35–50% of patients with stage I or II NSCLC develop recurrence and metastasis. This study was designed to investigate the anti-tumor activity of cyanidin (Cy) and peonidin (Pn) on NSCLC cells (SPCA-1). SPCA-1 cell proliferation, cell cycle and early apoptosis were investigated after treatment with Cy and Pn. The underlying signaling mechanism was also explored by detecting the levels of apoptosis-related proteins using enzyme-linked immunosorbent assay (ELISA). Cy and Pn inhibited the viability of SPCA-1 cells with an IC50 of 141.08 μg/mL and 161.31 μg/mL, respectively. Meanwhile, Cy and Pn induced cell cycle arrest at G2/M phase. Cy and Pn treatment significantly increased the levels of Bax, P53, and Caspase-3, while decreasing that of Bcl-2, thereby inhibiting the growth of SPCA-1 cells. In conclusion, Cy and Pn induced early apoptosis of NSCLC cells through regulation of the levels on Caspase-3, Bax, Bcl-2, and P53. These results suggest Cy and Pn as potential anticancer drugs for the treatment of lung cancer.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 3; 503-509
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anticancer activity of some new series of 2-(substituted)amino-1,3-thiazole derivatives
Autorzy:
Bakare, Safyah B.
Powiązania:
https://bibliotekanauki.pl/articles/780055.pdf
Data publikacji:
2019
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
Thiazole
Anticancer
Cell Cycle Analysis
Annexin V
FITC
Doxorubicin
Opis:
A series of thiazole derivatives were synthesized and structurally elucidated by IR, 1H NMR, 13C NMR, mass and elemental analyses. The prepared compounds were screened for their cytotoxic activity against Leukemia HL-60 cell line. Compound 4b was considered as the most promising antitumor candidate among the tested compounds. Mechanism of action of compound 4b evaluated by flow cytometric assay revealed cell cycle arrest at G2/M phase and pre-G1 apoptosis. The ratio of apoptosis was also determined. Moreover, compound 4b increased the concentration of caspase 3 by 4 fold more than untreated control.
Źródło:
Polish Journal of Chemical Technology; 2019, 21, 3; 19-25
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of NucleoCounter for the comprehensive assessment of murine cultured neurons during infection with Equine Herpesvirus type 1 (EHV-1)
Autorzy:
Chodkowski, M.
Serafińska, I.
Brzezicka, J.
Bańbura, M.W.
Cymerys, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087859.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
NucleoCounter NC-3000
viability
cell cycle
apoptosis
neurons
EHV-1
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 831-834
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
LaCl3 induces genomic DNA instability and increases DNA methylation levels in wheat roots
Autorzy:
Lei, X.
Ma, K.
Zhang, F.
Powiązania:
https://bibliotekanauki.pl/articles/2117694.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
cell cycle
Ca 2+ -channel blocker
RAPD
CRED-RA
Triticum aestivum
Opis:
Accumulation of LaCl3 , a well-known Ca 2+ -channel blocker, can inhibit plant growth. However, the current understanding of its effects on gene expression is limited. In this paper, different concentrations of LaCl 3 (0, 0.5, 1.0, 1.5, 2.0 mM) were used to treat germinated wheat (Triticum aestivum L.) seeds for 24 h. The degree of root growth inhibition gradually increased with increasing LaCl 3 concentration. qRT-PCR analysis revealed that the expression of several key genes related to the cell cycle process, such as pcna, mcm2, rdr and cyclin B, were significantly down-regulated. Further analysis of genomic DNA instability using Random Amplified Polymorphic DNA (RAPD) and methylation levels by Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) analysis indicated a significant increase in genomic DNA polymorphisms and methylation levels. The results of this study verified that the reasons why LaCl3 treatment can inhibit the growth of wheat roots are as follows: interference in the normal progression of the cell cycle, induction of genomic DNA instability and increase in DNA methylation levels.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2021, 63, 1; 31-41
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anticancer activity of some polyamine derivatives on human prostate and breast cancer cell lines
Autorzy:
Szumilak, Marta
Galdyszynska, Malgorzata
Dominska, Kamila
Stanczak, Andrzej
Piastowska-Ciesielska, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1038652.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polyamine derivatives
prostate cancer
breast cancer
mitochondrial potential
cell cycle
apoptosis
Opis:
The aim of this study was to expand our knowledge about anticancer activity of some polyamine derivatives with quinoline or chromane as terminal moieties. Tested compounds were evaluated in vitro towards metastatic human prostate adenocarcinoma (PC3), human carcinoma (DU145) and mammary gland adenocarcinoma (MCF7) cell lines. Cell viability was estimated on the basis of mitochondrial metabolic activity using water-soluble tetrazolium WST1 to establish effective concentrations of the tested compounds under experimental conditions. Cytotoxic potential of polyamine derivatives was determined by the measurement of lactate dehydrogenase activity released from damaged cells, changes in mitochondrial membrane potential, the cell cycle distribution analysis and apoptosis assay. It was revealed that the tested polyamine derivatives differed markedly in their antiproliferative activity. Bischromane derivative 5a exhibited a rather cytostatic than cytotoxic effect on the tested cells, whereas quinoline derivative 3a caused changes in cell membrane integrity, inhibited cell cycle progression, as well as induced apoptosis of prostate and breast cancer cells which suggest its potential application in cancer therapy.
Źródło:
Acta Biochimica Polonica; 2017, 64, 2; 307-313
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biogeneza rzęski pierwotnej
Biogenesis of the primary cilium
Autorzy:
Poprzeczko, Martyna
Joachimiak, Ewa
Włoga, Dorota
Fabczak, Hanna
Powiązania:
https://bibliotekanauki.pl/articles/1033949.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Tematy:
cell cycle
ciliogenesis
ciliopathies
primary cilium
ciliogeneza
ciliopatie
cykl komórkowy
rzęska pierwotna
Opis:
Rzęski pierwotne, struktury zbudowane na bazie cytoszkieletu mikrotubularnego, występują na powierzchni niemal wszystkich komórek ssaczych. Dzięki licznym receptorom błonowym, rzęski pierwotne pośredniczą w odbieraniu i przekazywaniu bodźców ze środowiska do wnętrza komórki, i tym samym odgrywają niezwykle ważną rolę w prawidłowym rozwoju i funkcjonowaniu większości tkanek i narządów. Tworzenie rzęski (ciliogeneza) to złożony, wieloetapowy i wielopoziomowo regulowany proces ściśle związany z cyklem komórkowym. Mutacje w genach kodujących białka strukturalne lub odpowiedzialne za prawidłowe funkcjonowanie rzęsek, jak również, regulujące przebieg ciliogenezy są przyczyną ich dysfunkcji, prowadzącej w efekcie do wielonarządowych chorób zwanych ciliopatiami.
Cilia are highly specialized, microtubule-based protrusions, extended on cell surface in almost all mammalian cell types. They function as cell antennae that receive and transmit signals from the environment to the cell body. Cilia formation, so-called ciliogenesis is strictly controlled at multiple levels by a number of proteins, and correlated with the cell cycle progression. Cilia dysfunctions cause a wide range of human diseases, called ciliopathies. Moreover, ciliary defects may lead to obesity and cancer. In this article, we summarize current knowledge concerning cilia function and structure, regulation of ciliogenesis, and the most important signaling pathways and diseases affected by cilia dysfunction.
Źródło:
Kosmos; 2018, 67, 1; 179-193
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cyclic AMP deficiency stimulates a stress condition in tobacco BY-2 cells
Autorzy:
Sgobba, A.
Sabetta, W.
Blanco, E.
Paradiso, A.
Viggiano, L.
De Pinto, M.
Powiązania:
https://bibliotekanauki.pl/articles/80076.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
cyclic amphipathic peptide
stress condition
tobacco
BY-2 cell
cell cycle progression
plant
subunit
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ascorbate-glutathione dependent redox homeostasis in stress response and cell cycle control
Autorzy:
De Gara, L.
Locato, V.
Cimini, S.
Novo Uzal, E.
De Pinto, M.C.
Powiązania:
https://bibliotekanauki.pl/articles/80263.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
ascorbate
glutathione
homeostasis
signalling pathway
programmed cell death
hydrogen peroxide
stress response
cell cycle
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lipid peroxidation and cell cycle signaling : 4-hydroxynonenal, a key molecule in stress mediated signaling.
Autorzy:
Yang, Yusong
Sharma, Rajendra
Sharma, Abha
Awasthi, Sanjay
Awasthi, Yogesh
Powiązania:
https://bibliotekanauki.pl/articles/1043608.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glutathione S-transferase
RLIP76
4-hydroxynonenal
RalBP1
cell cycle signaling
apoptosis
Opis:
Role of lipid peroxidation products, particularly 4-hydroxynonenal (4-HNE) in cell cycle signaling is becoming increasingly clear. In this article, recent studies suggesting an important role of 4-HNE in stress mediated signaling for apoptosis are critically evaluated. Evidence demonstrating the modulation of UV, oxidative stress, and chemical stress mediated apoptosis by blocking lipid peroxidation by the α-class glutathione S-transferases (GSTs) is presented which suggest an important role of these enzymes in protection against oxidative stress and a role of lipid peroxidation products in stress mediated signaling. Overexpression of 4-HNE metabolizing GSTs (mGSTA4-4, hGSTA4-4, or hGST5.8) protects cells against 4-HNE, oxidative stress (H2O2 or xanthine/xanthine oxidase), and UV-A mediated apoptosis by blocking JNK and caspase activation suggesting a role of 4-HNE in the mechanisms of apoptosis caused by these stress factors. The intracellular concentration of 4-HNE appears to be crucial for the nature of cell cycle signaling and may be a determinant for the signaling for differentiation, proliferation, transformation, or apoptosis. The intracellular concentrations of 4-HNE are regulated through a coordinated action of GSTs (GSTA4-4 and hGST5.8) which conjugate 4-HNE to GSH to form the conjugate (GS-HNE) and the transporter 76 kDa Ral-binding GTPase activating protein (RLIP76), which catalyze ATP-dependent transport of GS-HNE. A mild stress caused by heat, UV-A, or H2O2 with no apparent effect on the cells in culture causes a rapid, transient induction of hGST5.8 and RLIP76. These stress preconditioned cells acquire ability to metabolize and exclude 4-HNE at an accelerated pace and acquire relative resistance to apoptosis by UV and oxidative stress as compared to unconditioned control cells. This resistance of stress preconditioned cells can be abrogated by coating the cells with anti-RLIP76 antibodies which block the transport of GS-HNE. These studies and previous reports discussed in this article strongly suggest a key role of 4-HNE in stress mediated signaling.
Źródło:
Acta Biochimica Polonica; 2003, 50, 2; 319-336
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
IAA and BAP affect protein phosphorylation-dependent processes during sucrose-mediated G1 to S and G2 to M transitions in root meristem cells of Vicia faba
Autorzy:
Polit, J T
Maszewski, J.
Rosiak, M.
Powiązania:
https://bibliotekanauki.pl/articles/58127.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Vicia faba
cell cycle
sucrose
auxin
protein phosphorylation
cytokinin
control point
meristem
root
Opis:
In carbohydrate-starved root meristems of Vicia faba subsp. minor, the expression of two Principal Control Points located at the final stages of the G1 (PCP1) and G2 (PCP2) phases has been found to be correlated with a marked decrease of protein phosphorylation within cell nuclei, nucleoli and cytoplasm. Adopting the same experimental model in our present studies, monoclonal FITC conjugated antibodies that recognize phosphorylated form of threonine (αTPab-FITC) were used to obtain an insight about how the indole-3-acetic acid (IAA), benzyl-6-aminopurine (BAP), and the mixture of both phytohormones influence the time-course changes in an overall protein phosphorylation during sucrose-mediated PCP1→S and PCP2→M transitions. Unsuspectedly, neither IAA, BAP, nor the mixture of both phytohormones supplied in combination with sucrose did up-regulate protein phosphorylation. However using the block-and-release method, it was shown that root meristems of Vicia provided with sucrose alone indicated higher levels of αTPab-FITC. Contrarily, phytohormones supplied in combination with sucrose induced apparent decline in phosphorylation of cell proteins, which - when compared with the influence of sucrose alone - became increasingly evident in time. Thus, it seems probable, that a general decline in the amount of αTPab-FITC labeled epitopes may overlay specific phosphorylations and dephosphorylations governed by the main cell cycle kinases and phosphatases.
Źródło:
Acta Societatis Botanicorum Poloniae; 2004, 73, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of roscovitine, a selective CDK inhibitor, on cancer cells: bi-functionality increases its therapeutic potential
Autorzy:
Węsierska-Gądek, Józefa
Borza, Andreea
Komina, Oxana
Maurer, Margarita
Powiązania:
https://bibliotekanauki.pl/articles/1040546.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
inhibitors of cyclin-dependent kinases
cell cycle arrest
cyclin-dependent kinases
apoptosis
roscovitine
Opis:
Increased expression and activity of proteins driving cell cycle progression as well as inactivation of endogenous inhibitors of cyclin-dependent kinases (CDKs) enhance the proliferative potential of cells. Escape of cells during malignant transformation from the proper cell cycle control rendering them independent from growth factors provides rationale for therapeutic targeting of CDKs. Exposure of rapidly growing human MCF-7 breast cancer and HeLa cervix cancer cells to roscovitine (ROSC), a selective inhibitor of CDKs, inhibits their proliferation by induction of cell cycle arrest and/or apoptosis. The outcome strongly depends on the intrinsic traits of the tumor cells, on their cell cycle status prior to the onset of treatment and also on ROSC concentration. At lower dose ROSC primarily inhibits the cell cycle-related CDKs resulting in a strong cell cycle arrest. Interestingly, ROSC arrests asynchronously growing cells at the G2/M transition irrespective of the status of their restriction checkpoint. However, the exposure of cancer cells synchronized after serum starvation in the late G1 phase results in a transient G1 arrest only in cells displaying the intact G1/S checkpoint. At higher dosage ROSC triggers apoptosis. In HeLa cells inhibition of the activity of CDK7 and, in consequence, that of RNA polymerase II is a major event that facilitates the initiation of caspase-dependent apoptosis. In contrast, in the caspase-3-deficient MCF-7 breast cancer cells ROSC induces apoptosis by a p53-dependent pathway. HIPK2-mediated activation of the p53 transcription factor by phosphorylation at Ser46 results in upregulation of p53AIP1 protein. This protein after de novo synthesis and translocation into the mitochondria promotes depolarization of the mitochondrial membrane.
Źródło:
Acta Biochimica Polonica; 2009, 56, 3; 495-501
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Human SUV3 helicase regulates growth rate of the HeLa cells and can localize in the nucleoli
Autorzy:
Szewczyk, Maciej
Fedoryszak-Kuśka, Natalia
Tkaczuk, Katarzyna
Dobrucki, Jurek
Waligórska, Agnieszka
Stępień, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1038704.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
SUV3 helicase
SUPV3L1
dual targeting
nucleolus
mitochondria
cell cycle
Opis:
The human SUV3 helicase (SUV3, hSUV3, SUPV3L1) is a DNA/RNA unwinding enzyme belonging to the class of DexH-box helicases. It localizes predominantly in the mitochondria, where it forms an RNA-degrading complex called mitochondrial degradosome with exonuclease PNP (polynucleotide phosphorylase). Association of this complex with the polyA polymerase can modulate mitochondrial polyA tails. Silencing of the SUV3 gene was shown to inhibit the cell cycle and to induce apoptosis in human cell lines. However, since small amounts of the SUV3 helicase were found in the cell nuclei, it was not clear whether the observed phenotypes of SUV3 depletion were of mitochondrial or nuclear origin. In order to answer this question we have designed gene constructs able to inhibit the SUV3 activity exclusively in the cell nuclei. The results indicate that the observed growth rate impairment upon SUV3 depletion is due to its nuclear function(s). Unexpectedly, overexpression of the nuclear-targeted wild-type copies of the SUV3 gene resulted in a higher growth rate. In addition, we demonstrate that the SUV3 helicase can be found in the HeLa cell nucleoli, but it is not detectable in the DNA-repair foci. Our results indicate that the nucleolar-associated human SUV3 protein is an important factor in regulation of the cell cycle.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 177-181
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 41

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies