- Tytuł:
- A novel method for thermodynamic study on the binding of milk carrier protein of BLG-A with Cr+3
- Autorzy:
-
Behbehania, G.R.
Divsalar, A.
Saboury, A.A. - Powiązania:
- https://bibliotekanauki.pl/articles/778861.pdf
- Data publikacji:
- 2009
- Wydawca:
- Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
- Tematy:
-
BLG-A
Cr+3
izotermiczna kalorymetria miareczkowa
kalorymetria
Cr3+
isothermal titration calorimetry
binding parameters - Opis:
- Thermodynamics of the interaction between Cr3+ with β-lactoglobulin type A (BLG-A) was investigated at pH 7.0 and 37°C by isothermal titration calorimetry. A new method to follow the effect of Cr3+ on the stability of BLG-A was introduced. The new solvation model was used to reproduce the enthalpies of BLG-A+ Cr3+ interactions over the whole range of Cr3+ concentrations. The solvation parameters recovered from the new equation are attributed to the structural change of BLG-A and its biological activity. The results obtained indicate that there is a set of two identical binding sites for Cr3+ ions with positive cooperativity. The association equilibrium constants are 14.39 and 0.49 mM-1 for the first and second binding site, respectively. The enthalpy of binding for one mole of Cr+3 ion to one mole of the binding site on BLG-A (ΔH=104.60 kJ mol-1) is obtained.
- Źródło:
-
Polish Journal of Chemical Technology; 2009, 11, 4; 24-29
1509-8117
1899-4741 - Pojawia się w:
- Polish Journal of Chemical Technology
- Dostawca treści:
- Biblioteka Nauki
Artykuł