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    Wyświetlanie 1-8 z 8
    Tytuł:
    The effect of NAA and BAP on the multiplication of chamomile (Matricaria chamomilla L.) callus tissue in vitro
    Autorzy:
    Mazur, Paweł
    Kulpa, Danuta
    Powiązania:
    https://bibliotekanauki.pl/articles/1075653.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Campyliaceae
    Chamomile
    Matricaria chamomilla
    callus tissue
    growth regulators
    in vitro
    multiplication
    Opis:
    The aim of the study was to determine the effect of the additive on NAA and BAP on the formation of callus tissue on leaf chamomile explants, cultivar, and to determine the optimal content of said growth regulators for the initiation of callus tissue in vitro. During the experiment fragments of chamomile from the collection of the Department of Genetics, Breeding and Biotechnology of Plant were laid on a medium with mineral composition according to Murashige and Skoog - MS (1962) enriched with auxin NAA in concentrations of 3 and 5 mg·dm-3 and cytokinin BAP in a concentration of 1, 2 , 5, 7 mg·dm-3. Based on the conducted experiment it was found that the initiation of callus chamomile tissue is possible in the case of using leaf explants lined with MS in the combination of auxin NAA in concentration 3 or 5 mg·dm-3 and BAP from 1 to 7 mg·dm-3. The propagation of chamomile callus tissue should be carried out on MS proliferating medium enriched with NAA at a rate of 3 mg·dm-3 and BAP in an amount of 7 mg·dm-3. Explants laid on a medium with this composition develop a large mass of callus tissue.
    Źródło:
    World Scientific News; 2019, 125; 245-251
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method
    Proantocyjanidyny w tkankach kalusowych i w transformowanych korzeniach Rhodiola kirilowii i Rhodiola rosea - oznaczenie za pomocą metody UPLC-MS/MS
    Autorzy:
    Gryszczynska, A.
    Krajewska-Patan, A.
    Dreger, M.
    Buchwald, W.
    Pietrosiuk, A.
    Zych, M.
    Karasiewicz, M.
    Bogacz, A.
    Kujawski, R.
    Furmanowa, M.
    Czerny, B.
    Mielcarek, S.
    Gazda, P.
    Mrozikiewicz, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/72184.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    proanthocyanidin
    Rhodiola kirilowii
    Rhodiola rosea
    callus tissue
    transformed root
    root
    determination
    ultra performance liquid chromatography-tandem mass spectrometric method
    Opis:
    Several species of Rhodiola genus (Crassulaceae family) like Rhodiola kirilowii and Rhodiola rosea are used in official or traditional medicine. The aim of this study was to determine qualitative and quantitative content of proanthocyanidins using ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) in the callus tissues and in the transformed roots (infected by Agrobacterium rhizogenes LBA 9402 strain) of R. kirilowii and R. rosea. This validated assay allows to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG). Our results concerning the material from in vitro cultivation of R. kirilowii and R. rosea indicate that R. rosea callus can be a better source of catechin when compared with other tested materials, especially when the content of (-)-gallate epigallocatechin is taken under consideration (3.429 mg/100 g of dry powdered material). The application of UPLC MS/MS method allowed to determine the content of proanthocyanidins in tested samples with satisfactory precision and can be used in the phytochemical investigations of Rhodiola sp. in vitro cultivated tissues.
    Niektóre gatunki z rodzaju Rhodiola (rodzina Crassulaceae), jak Rhodiola kirilowii i Rhodiola rosea, są stosowane w medycynie oficjalnej lub ludowej. Celem przedstawionych badań było oznaczenie jakościowe i ilościowe proantocyjanidyn w tkankach kalusowych i w transformowanych (za pomocą szczepu Agrobacterium rhizogenes LBA 9402) korzeniach Rhodiola kirilowii i Rhodiola rosea przy zastosowaniu metodyki wykorzystującej ultrasprawny chromatograf cieczowy sprzężony z tandemowym spektrometrem mas (metoda UPLC MS/MS). Ta zawalidowana metodyka pozwoliła na określenie stężeń pięciu flawan-3-oli: (+)-katechiny, (-)-epikatechiny, (-)-epigalokatechiny, galusanu (-)-epikatechiny (ECG) oraz galusanu (-)-epigalokatechiny (EGCG). Przedstawione w pracy wyniki dotyczące materiału pochodzącego z kultur in vitro R. kirilowii i R. rosea wskazują, że kalus R. rosea jest lepszym źródłem katechin w porównaniu do pozostałych badanych surowców, szczególnie, gdy bierze się pod uwagę zawartość galusanu epigalokatechiny (3.429 mg/100 g suchego sproszkowanego surowca). Zastosowanie opracowanej metodyki z wykorzystaniem ultrasprawnego chromatografu cieczowego sprzężonego z tandemowym spektrometrem mas pozwoliło z zadawalającą precyzją oznaczyć zawartości proantocyjanidyn w analizowanych próbkach. Metoda ta może być stosowana w fitochemicznych badaniach hodowanych in vitro tkanek rodzaju Rhodiola.
    Źródło:
    Herba Polonica; 2012, 58, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
    Autorzy:
    Siong, P.K.
    Taha, R.M.
    Rahiman, F.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/19314.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    somatic embryogenesis
    plant regeneration
    hypocotyl
    leaf explant
    Brassica oleracea var.botrytis
    cauliflower
    embryogenic callus
    callus
    tissue culture
    Opis:
    We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
    Autorzy:
    Nawrot-Chorabik, K
    Powiązania:
    https://bibliotekanauki.pl/articles/41643.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    silver fir
    Abies alba
    tissue culture
    embryogenic suspensor mass
    somatic embryogenesis
    callus induction
    differentiation
    Opis:
    The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
    Źródło:
    Dendrobiology; 2008, 59; 31-40
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
    Autorzy:
    Li, J.
    Kang, Y.
    Qiang, S.
    Peng, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/56457.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    propagation
    goldenrod
    Solidago canadensis
    leaf
    nodal explant
    callus formation
    micropropagation
    adventitious shoot
    tissue culture
    Opis:
    Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2012, 81, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somaclonal variation in winter wheat [Triticum aestivum L.]: frequency, occurrence and inheritance
    Autorzy:
    Cheng, X Y
    Gao, M.W.
    Liang, Z.Q.
    Liu, G.Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044461.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    inheritance
    tissue culture
    Triticum aestivum
    occurrence
    in vitro
    winter wheat
    gene mutation
    plant breeding
    callus induction
    frequency
    somaclonal variation
    wheat
    embryo
    Opis:
    Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. General responses of regenerated plants were investigated and a total of 7142 R₂ spike lines from 1593 R₁ plants were assessed in the field for somaclonal variants in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic inheritance. From regenerated plantlets, 81% survived and 63% produced fertile plants. Forms with reduced plant height, length of spike and other morphological abnormalities were found in this progeny. Populations of R₁ plants were highly variable due mainly to the physiological disturbances resulting from the in vitro process. Overall somaclonal variation frequencies were 14.2% per plant basis and 5.3% per R₂ spike basis. The variants were similar in the three different R₂ generations with predominant variants being negative in plant height, maturity, awns, spike type and plant type. Both uniform R₂ variant families and spike lines were found in addition to the segregating variants which constituted the majority. On average, in a variant family or line, 18% and 14% of their component lines and plants were variants, respectively. Inheritability was demonstrated for the uniform variant families and spike lines as well as segregated variants. Of those 134 selections, about 70% were classified as inheritable. Both recessive and dominant gene mutations at one, two or three loci were evident in some variants as suggested by the segregating data.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 59-72
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
    Autorzy:
    Ullrich, S E
    Kleinhofs, A.
    Hou, L.
    Jones, B.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044453.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    tissue culture
    doubled haploid
    mutagenesis
    breeding programme
    anther culture
    in vitro
    callus culture
    somaclonal variation
    barley
    mutation
    Hordeum vulgare
    embryo
    molecular biology
    Opis:
    Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 49-58
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)
    Kwitnienie i mikropropagacja in vitro lisianthusa (Eustoma grandiflorum) w reakcji na regulatory wzrostu roslin (NAA i BA)
    Autorzy:
    Kaviani, B.
    Zamiraee, F.
    Zanjani, S.B.
    Tarang, A.
    Torkashvand, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542950.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro culture
    ornamental plant
    flowering
    micropropagation
    Lisianthus
    Eustoma grandiflorum
    plant response
    plant growth regulator
    NAA potassium salt
    axillary bud
    callus
    plant tissue culture
    Opis:
    In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
    Kwitnienie i mikropropagacja in vitro są użyteczne w programach hodowli roślin oraz produkcji komercyjnej ważnych roślin ozdobnych. Warunki in vitro, łącznie ze składnikami pożywek, rodzajem, stężeniem oraz proporcją regulatorów wzrostu roślin, a także warunkami hodowli, w sposób istotny wpływają na kwitnienie i mikropropagację in vitro. Nie istnieje żadne badanie dotyczące kwitnienia in vitro lisanthiusa (Eustoma grandiflorum). W niniejszym badaniu opracowano kwitnienie i wysoką częstotliwość mikropropagacji in vitro dla E. grandiflorum, który jest rośliną ozdobną. Mikropropagacja jest skutecznym narzędziem rozmnażania roślin ozdobnych na dużą skalę. Celem niniejszego badania była ocena wpływu różnych stężeĔ NAA i BA na mikropropagację i kwitnienie lisianthiusa in vitro. Używana pożywka hodowlana została wzbogacona za pomocą 0; 0,1; 0,2 i 2 mg L-1 NAA i BA. Przy powstawaniu eksplantów największa długość łodygi (2,07 cm na roślinę) była uzyskana na pożywce uzupełnionej o 0,1 mg L-1 BA (bez NAA). Maksymalna liczba łodyg (5,80 na roślinę) została wytworzona na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,2 mg L-1 NAA. Eksplanty pączków na pożywce hodowlanej zawierającej 0,2 mg L-1 NAA (bez BA) oraz 0,1 mg L-1 NAA wraz z 2 mg L-1 BA wytworzyły maksymalną liczbę węzłów (3,20 na roślinę). Największą liczbę korzeni (14,53 na roślinę) oraz największą długość korzenia (3,87 na roślinę) zaobserwowano na 0,2 mg L-1 NAA bez BA jak również 0,2 mg L-1 BA plus 0,2 mg L-1 NAA oraz 0,2 mg L-1 BA bez NAA. Eksplanty tworzyły kwiat na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,1 mg L-1 NAA bez przeniesienia kalusa. Kwiat był tworzony z kalusa na pożywce zawierającej 0,1 mg L-1 BA wraz z 2 mg L-1 NAA. Zregenerowane rośliny wykazały 98% przeżycie w szklarni podczas aklimatyzacji. Zaaklimatyzowane rośliny były morfologicznie podobne to swych roślin macierzystych.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 145-155
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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