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    Wyświetlanie 1-13 z 13
    Tytuł:
    Plasma membrane Ca2+ -ATPase in excitable and nonexcitable cells.
    Autorzy:
    Żylińska, Ludmiła
    Soszyński, Mirosław
    Powiązania:
    https://bibliotekanauki.pl/articles/1044284.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    calcium homeostasis
    brain
    erythrocytes
    plasma membrane Ca2+ -ATPase
    Opis:
    There is a significant number of data confirming that the maintenance of calcium homeostasis in a living cell is a complex, multiregulated process. Calcium efflux from excitable cells (i.e., neurons) occurs through two main systems - an electrochemically driven Na+/Ca2+ exchanger with a low Ca2+ affinity (K0.5 = 10-15 μM), and a plasmalemmal, specific Ca2+-ATPase, with a high Ca2+ affinity (K0.5 < 0.5-1 μM), whereas in nonexcitable cells (i.e., erythrocytes) the calcium pump is the sole system responsible for the extrusion of calcium ions. The plasma membrane Ca2+-ATPase (PMCA) is a ubiquitously expressed protein, and more than 26 transcripts of four PMCA genes are distributed in a tissue specific manner. Differences in the structure and localization of PMCA variants are thought to correlate with specific regulatory properties and may have consequences for proper cellular Ca2+ signaling. The regulatory mechanisms of calcium pump activity have been studied extensively, resulting in a new view of the functioning of this important molecule in the membranes.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 3; 529-539
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Regulation of Ca2+ release from internal stores in cardiac and skeletal muscles.
    Autorzy:
    Wrzosek, Antoni
    Powiązania:
    https://bibliotekanauki.pl/articles/1044313.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Ca2+-induced Ca2+ release
    calcium channels
    dihydropyridine receptor
    calcium sparks
    excitation-contraction coupling
    ryanodine receptor
    Opis:
    It is widely accepted that Ca2+ is released from the sarcoplasmic reticulum by a specialized type of calcium channel, i.e., ryanodine receptor, by the process of Ca2+-induced Ca2+ release. This process is triggered mainly by dihydropyridine receptors, i.e., L-type (long lasting) calcium channels, directly or indirectly interacting with ryanodine receptor. In addition, multiple endogenous and exogenous compounds were found to modulate the activity of both types of calcium channels, ryanodine and dihydropyridine receptors. These compounds, by changing the Ca2+ transport activity of these channels, are able to influence intracellular Ca2+ homeostasis. As a result not only the overall Ca2+ concentration becomes affected but also spatial distribution of this ion in the cell. In cardiac and skeletal muscles the release of Ca2+ from internal stores is triggered by the same transport proteins, although by their specific isoforms. Concomitantly, heart and skeletal muscle specific regulatory mechanisms are different.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 3; 705-723
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Changes of Calcium and Magnesium Content in Biomass of Goats Rue (Galega orientalis Lam.) during Vegetation
    Zmiany zawartości wapnia i magnezu w biomasie rutwicy wschodniej (Galega orientalis Lam.) podczas wegetacji
    Autorzy:
    Symanowicz, B.
    Kalembasa, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/387986.pdf
    Data publikacji:
    2012
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    rutwica wschodnia (Galega orientalis Lam.)
    wapń
    magnez
    rok uprawy
    faza rozwojowa
    biomasa
    liście
    łodyga
    stosunek Ca : Mg
    fodder galega (Galega orientalis Lam.)
    calcium
    magnesium
    year of cultivation
    development phase
    biomass
    leaves
    stem
    Ca : Mg ratio
    Opis:
    Calcium and magnesium are among macroelements, both in plant and animal feeding. Green forage, especially that obtained from legume plants, is their main source in ruminants’ feed. The optimum content of those elements in fodder positively affects its quality. The aim of this study was to trace changes in calcium and magnesium content in fodder galega (Galega orientalis) during the vegetation period, depending on the year of cultivation and development phase. The results are based on two field experiments, conducted for the third and seventh year. Samples were taken during the harvest from 1 m2 of the field during the following development phases: budding, start of blossoming, full bloom, end of blossoming and full ripeness. Subsequently, the samples were dried and ground. Calcium and magnesium were determined by the ICP-AES method following dry mineralisation. Statistical calculations have revealed significant variation in calcium and magnesium content in fodder galega (Galega orientalis), depending on the year of cultivation and development phase. The average calcium content in dry matter of the test plant was equal to 15.57 g kg–1, while that of magnesium was 2.54 g kg–1. The largest amounts of calcium and magnesium were found in the leaves of the test plant in the third year of cultivation. Considering different development phases of fodder galega, it can be concluded that the highest level of calcium was determined at the end of the blossoming phase and the highest level of magnesium – during the full ripeness phase. The average Ca : Mg ratio was equal to 6.12 : 1.
    Wapń i magnez należą do podstawowych składników mineralnych organizmów zwierzęcych. Są one niezbędne do funkcjonowania całego organizmu i metabolizmu komórek. Podstawowym źródłem Ca i Mg w żywieniu zwierząt przeżuwających są zielonki, szczególnie roślin bobowatych. Optymalna zawartość tych składników w paszy korzystnie wpływa na jej jakość. Celem przeprowadzonych badań było prześledzenie zmian w zawartości wapnia i magnezu w biomasie rutwicy wschodniej w zależności od roku uprawy i fazy rozwojowej. Wyniki badań uzyskano na podstawie dwóch doświadczeń polowych prowadzonych trzeci i siódmy rok. Podczas zbioru pobrano próbki z 1 m2 w następujących fazach rozwojowych: pąkowanie, początek kwitnienia, pełnia kwitnienia, koniec kwitnienia i dojrzałość pełna. Następnie próbki te wysuszono i rozdrobniono. Wapń i magnez oznaczono metodą ICP-AES, po mineralizacji "na sucho". Obliczenia statystyczne wykazały istotne zróżnicowanie w zawartości wapnia i magnezu w biomasie rutwicy wschodniej (Galega orientalis Lam.) w zależności od roku uprawy i fazy rozwojowej. Średnia zawartość wapnia w suchej masie rośliny testowej wynosiła 15,57 g kg-1 a magnezu 2,54 g kg-1. Największe ilości wapnia i magnezu oznaczono w liściach rośliny testowej w trzecim roku uprawy. Rozpatrując poszczególne fazy rozwojowe rutwicy wschodniej, należy stwierdzić, że w fazie koniec kwitnienia oznaczono najwięcej wapnia, natomiast magnezu w fazie dojrzałości pełnej. Średni stosunek Ca : Mg ukształtował się na poziomie 6,12 : 1.
    Źródło:
    Ecological Chemistry and Engineering. A; 2012, 19, 7; 689-698
    1898-6188
    2084-4530
    Pojawia się w:
    Ecological Chemistry and Engineering. A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prolaktyna i inne regulatory wchłaniania wapnia
    Prolactin and other regulators of calcium absorption
    Autorzy:
    Dolińska, Barbara
    Łopata, Katarzyna
    Ryszka, Florian
    Powiązania:
    https://bibliotekanauki.pl/articles/1038579.pdf
    Data publikacji:
    2012
    Wydawca:
    Śląski Uniwersytet Medyczny w Katowicach
    Tematy:
    wapń (ca)
    wchłanianie
    prolaktyna (prl)
    osteoporoza
    calcium (ca)
    absorption
    prolactin
    osteoporosis
    Opis:
    Absorption of calcium is regulated by many endogenous and exogenous factors. Prolactin is important regulator. Numerous studies indicate that prolactin can stimulate calcium transport in the small intestine in vitro and in vivo. This is especially important during the growing demand for calcium in pregnancy and in lactation. There is report the role of prolactin in maintaining calcium homeostasis.
    Wchłanianie wapnia regulują liczne czynniki endogenne i egzogenne, m.in. prolaktyna. Jak wynika z wielu badań, może ona stymulować transport wapnia w jelicie cienkim w warunkach in vitro oraz in vivo. Jest to szczególnie ważne w okresie wzrostu zapotrzebowania na wapń w okresie ciąży i laktacji. W licznych doniesieniach wskazano na rolę prolaktyny w zachowaniu homeostazy wapniowej.
    Źródło:
    Annales Academiae Medicae Silesiensis; 2012, 66, 1; 52-56
    1734-025X
    Pojawia się w:
    Annales Academiae Medicae Silesiensis
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Content of calcium and phosphorus and the Ca:P ratio is selected species of leguminous and herbaceous plants
    Autorzy:
    Grzegorczyk, S.
    Alberski, J.
    Olszewska, M.
    Grabowski, K.
    Bałuch-Małecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1190201.pdf
    Data publikacji:
    2017
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
    Tematy:
    grasslands
    legumes
    herbs
    calcium
    phosphorus
    Ca:P
    Opis:
    Green fodder plays a key role in nutrition of ruminants, and its quality is determined by the species composition of meadow and pasture swards. Legumes and herbs are generally more abundant in nutrients, especially minerals, than grasses. Calcium (Ca) and phosphorus (P) are particularly important for animal health. The dietary levels of Ca and P should be balanced to increase their availability and utilization. The aim of this study was to determine the content of calcium and phosphorus and the Ca:P ratio in selected legume and herb species from extensively used grasslands in the Olsztyn Lakeland. The study covered the following species of leguminous plants: Trifolium pratense, Trifolium repens, Lotus corniculatus, Lathyrus pratensis, Lotus uliginosus and Vicia cracc, and herbaceous plants: Taraxacum officinale, Achillea millefolium, Plantago lanceolata, Alchemilla vulgaris, Heracleum sibiricum and Cirsium oleraceum. The investigated legumes and herbs differed significantly in their Ca and P content and the Ca: P ratio. All the species were abundant in Ca, and two taxa: Cirsium oleraceum and Heracleum sibiricum, were characterized by particularly high levels of calcium. The highest levels of phosphorus were noted in Heracleum sibiricum, whereas the lowest content of P was determined in Alchemilla vulgaris, Cirsium oleraceum and Trifolium pratense. Cirsium oleraceum was abundant in Ca and deficient in P, hence it was distinguished by the widest Ca:P ratio. Legumes and herbs are valuable components of meadow sward and a rich source of minerals, in particular Ca, in animal diets.
    Źródło:
    Journal of Elementology; 2017, 22, 2; 663-669
    1644-2296
    Pojawia się w:
    Journal of Elementology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development and SEM/EDS characterisation of porous coatings enriched in magnesium and copper obtained on titanium by PEO with ramp voltage
    Autorzy:
    Rokosz, Krzysztof
    Hryniewicz, Tadeusz
    Pietrzak, Kornel
    Dudek, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1179226.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    CP Titanium Grade 2
    Micro Arc Oxidation (MAO)
    Plasma Electrolytic Oxidation (PEO)
    calcium nitrate Mg(NO3)2∙6H2O
    linear polarization
    ramp voltage
    zinc nitrate Ca(NO3)2∙3H2O
    Opis:
    In the present paper, the SEM and EDS results of porous and enriched in calcium and/or zinc coatings, which were obtained during 3-minute treatments by Plasma Electrolytic Oxidation/ Micro Arc Oxidation processes on CP Titanium Grade 2 at ramp potentials (liner polarization) from 0 up to 650 VDC in electrolytes containing 500 g Mg(NO3)2∙6H2O and/or 500 g Cu(NO3)2∙3H2O in 1 L H3PO4, are reported. It was found that the obtained coatings, dependent on the PEO process conditions, have pores with different shapes and diameters. The Mg/P and Cu/P ratios by atomic concentration are the same and equal to 0.09±0.01 (by wt %) | 0.11±0.01 (by at%) and/or 0.40±0.08 (by wt %) | 0.19±0.04 (by at%), respectively. That may testify the hydroxyapatite-like structures of Mg-Ti-PO43– and/or Cu-Ti-PO43– have been identified to occur.
    Źródło:
    World Scientific News; 2017, 80; 29-42
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Improvement of plant heat tolerance by modification of xanthophyll cycle activity
    Autorzy:
    Trojak, Magdalena
    Skowron, Ernest
    Powiązania:
    https://bibliotekanauki.pl/articles/1178460.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    ascorbic acid (AsA);
    calcium ions (Ca2+)
    dithiothreitol (DTT)
    heat stress
    plant protection products (PPPs)
    putrescine (Put)
    xanthophyll cycle
    Opis:
    Plants are sessile organisms hence environmental factors such as excessive light and high air temperature lead to significant reductions of their productivity and quality of gained yield. In fact, scientific and agriculture hubs make lots of efforts to improve crop tolerance to elevated temperature, selecting more tolerant varieties. We analyzed less expensive and highly efficient method to improve resistance of well-known cultivars of crop plant by reversible modification of xanthophyll cycle. It functions as a safety valve to adjust energy transfer and protects fragile structures of photosynthetic machinery from excessive light, especially accompanied by heat or water stress. Efficiency of modified xanthophyll cycle activity was measured after pre-treatment with four, chemically different regulators, with or without light illumination. Analyses were carried out on barley (Hordeum vulgare L.) cv. Zenek treated with ascorbic acid (AsA), dithiothreitol (DTT), putrescine (Put) and calcium ions (Ca2+). To measure the scale of thermal energy dissipation we traced energy transfer absorbed by PSII with PAM chlorophyll fluorescence technique. Results showed clear correlation between AsA (activator of violaxanthin de-epoxidase) treatment and stimulation of the Φ(NPQ) at increased temperature. DTT (inhibitor of violaxanthin de-epoxidase) decreased the cycle activity at 45 °C at the same time increasing its value at 35°C, caused by interaction with other enzymes. Action of Put (hydrogen ions buffer) concerned mainly a non regulated Φ(NO) energy quenching. We noticed that application of Ca(NO3)2 (Ca2+ source for enzyme activity) reduced the Φ(NPQ) at 45 °C and stimulated it at 25 °C. Obtained results confirmed postulated possibility of creating new type of plant protection products (PPPs) able to precisely manage natural mechanisms of heat resistance.
    Źródło:
    World Scientific News; 2017, 70, 2; 51-70
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SEM and EDS studies of porous coatings enriched in calcium and zinc obtained by PEO with ramp voltage
    Autorzy:
    Rokosz, Krzysztof
    Hryniewicz, Tadeusz
    Pietrzak, Kornel
    Powiązania:
    https://bibliotekanauki.pl/articles/1178366.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    CP Titanium Grade 2
    Micro Arc Oxidation (MAO)
    Plasma Electrolytic Oxidation (PEO)
    calcium nitrate Ca(NO3)2∙4H2O
    linear polarization
    ramp voltage
    zinc nitrate Zn(NO3)2∙6H2O
    Opis:
    In this work, the SEM and EDS results of porous and enriched in calcium or zinc coatings, which were obtained during 3-minute treatments using Plasma Electrolytic Oxidation (Micro Arc Oxidation) processes on CP Titanium Grade 2 at ramp potentials (linear polarization) from 0 up to 650 VDC in electrolytes containing 500 g Ca(NO3)2∙4H2O and/or 500 g Zn(NO3)2∙6H2O in 1 L H3PO4, are presented. It was found that obtained coatings have pores with different shapes and diameters. The Ca/P and Zn/P ratios by atomic concentration are the same and equal to 0.2, what may suggest hydroxyapatite-like structure (M,Ti)x(PO4)y, where M = {Ca, Zn}.
    Źródło:
    World Scientific News; 2017, 77, 2; 242-255
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SEM and EDS studies of selected porous coatings obtained on titanium by Plasma Electrolytic Oxidation
    Autorzy:
    Rokosz, Krzysztof
    Hryniewicz, Tadeusz
    Pietrzak, Kornel
    Dudek, Łukasz
    Malorny, Winfried
    Powiązania:
    https://bibliotekanauki.pl/articles/1178480.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    CP Titanium Grade 2
    Micro Arc Oxidation (MAO)
    Plasma Electrolytic Oxidation (PEO)
    calcium nitrate Ca(NO3)2∙4H2O
    copper nitrate Cu(NO3)2∙3H2O
    magnesium nitrate Mg(NO3)2∙6H2O
    Opis:
    In present work, SEM and EDS results of porous and enriched in calcium, copper, magnesium and phosphorus coatings, which were obtained during 3-minute treatments of Plasma Electrolytic Oxidation PEO (Micro Arc Oxidation MAO) processes on CP Titanium Grade 2 at two potentials, i.e. 500 VDC and 650 VDC, are presented. It was found that the voltage of that process influences the chemical composition of coatings shapes obtained on titanium in electrolytes containing calcium, copper and magnesium nitrates. The pores shapes of PEO coatings formed on titanium in electrolytes containing calcium, copper and magnesium nitrates are presented, too. In addition, it was also noted that with using of higher voltage (here 650 VDC) of PEO treatments, the higher Ca/P, Cu/P and Mg/P ratios were found than those after treatments at 500 VDC.
    Źródło:
    World Scientific News; 2017, 70, 2; 71-85
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis and characterisation of porous, calcium enriched coatings formed on Titanium via Plasma Electrolytic Oxidation
    Autorzy:
    Rokosz, Krzysztof
    Hryniewicz, Tadeusz
    Pietrzak, Kornel
    Powiązania:
    https://bibliotekanauki.pl/articles/1179160.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    85% orthophosphoric acid H3PO4
    CP Titanium Grade 2
    Micro Arc Oxidation (MAO)
    Plasma Electrolytic Oxidation (PEO)
    calcium nitrate tetrahydrate Ca(NO3)2∙4H2O
    Opis:
    The SEM and EDS study results of porous and calcium enriched coatings obtained via Plasma Electrolytic Oxidation (Micro Arc Oxidation) on the Commercial Purity Titanium Grade 2 samples, at three potentials 450 VDC, 550 VDC, 650 VDC in electrolytes containing 500 g Ca(NO3)2∙4H2O in 1000 mL H3PO4, are presented in the paper. In the same electrolyte three Titanium samples, one after other, were treated, with the PEO processing time equaling 3 minutes after each immersion. Based on SEM and EDS results, it was possible to state that all obtained coatings are porous and their calcium-to-phosphorus (Ca/P) ratio decreases with decreasing PEO voltage. In addition, it was found out that electrolyte aging results in decreasing Ca/P ratio, too.
    Źródło:
    World Scientific News; 2017, 83; 29-44
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Porous PEO coatings on titanium, obtained under DC regime, enriched in magnesium, calcium, zinc, and copper
    Autorzy:
    Rokosz, Krzysztof
    Hryniewicz, Tadeusz
    Pietrzak, Kornel
    Dudek, Łukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/1156239.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    plasma electrolytic oxidation (peo)
    micro arc oxidation (mao)
    cp titanium grade 2
    calcium nitrate ca(no3)2•4h2o
    magnesium nitrate mg(no3)2∙6h2o
    zinc nitrate zn(no3)2∙6h2o
    copper nitrate cu(no3)2∙3h2o
    Opis:
    In the present paper, the analysis of SEM and EDS results of porous and enriched in calcium, magnesium, zinc, copper and phosphorus coatings, obtained during 3-minute treatments performed by Plasma Electrolytic Oxidation (PEO)/Micro Arc Oxidation (MAO) processes on CP Titanium Grade 2, is presented. The PEO process was carried out at DC potentials of 500 VDC, 575 VDC, and 650 VDC in electrolytes containing 125 g Ca(NO3)2•4H2O, and 125 g Mg(NO3)2∙6H2O, and 125 g Zn(NO3)2∙6H2O, and 125 g Cu(NO3)2∙3H2O in 1 L H3PO4. It was found that obtained coatings have pores with different shapes and diameters and/or size/dimentions. The Ca/P, Mg/P, Zn/P, Cu/P, and M/P ratios (M=Ca+Mg+Zn+Cu) were equal to 0.074, 0.046, 0.056, 0.042, and 0.218, respectively. The highest value of each of these ratios was recorded for 650 VDC. It may be concluded that the obtained PEO coatings structures most likely are similar to hydroxyapatite-like structures, in which the Ca2+ may be replaced with Zn2+, Mg2+, Cu2+.
    Źródło:
    World Scientific News; 2018, 94, 2; 99-114
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs) in Kacang goat after vitrification
    Autorzy:
    Widjiati, W.
    Faizah, Z.
    Darsini, N.
    Hendrawan, V.F.
    Karima, H.N.
    Chotimah, C.
    Sumitro, S.B.
    Yustinasari, L.R.
    Kasman, A.A.M.N.
    Ntoruru, J.M.
    Luqman, E.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539072.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Calcium (Ca2+)
    confocal laser scanning microscope
    cumulus-oocyte complex (COCs)
    food production
    Kacang goat
    Opis:
    The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 19-26
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Effects of Various Organic Materials on Dactylis glomerata Yield and Content of Selected Macroelements
    Autorzy:
    Malinowska, Elżbieta
    Wiśniewska-Kadżajan, Beata
    Ostaszewska, Urszula
    Horaczek, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/27323835.pdf
    Data publikacji:
    2023
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    Dactylis glomerata
    yield
    organic materials
    K
    potassium
    Ca
    calcium
    Mg
    magnesium
    Opis:
    The aim of the experiment was to assess the effects of various organic materials on Dactylis glomerata yield, on the content of selected macroelements (K, Ca and Mg) and on K:Ca, K:Mg and K:(Ca + Mg) ratios. As a valuable forage plant, Dactylis glomerata (cocksfoot grass) is a common grass in Poland both in grassland and in arable fields. Its rapid spring growth and its resistance to drought, low temperatures, but also to frequent mowing and pests, makes it a common species in meadows, pastures and grassland, both permanent and alternating. In order to achieve the research goal, a three-year pot experiment was established in a greenhouse. The experiment was conducted in a completely random design, in four replications. In the autumn before the experiment, soil was mixed with organic materials (chicken manure, mushroom substrate and rye straw) and put into pots. To selected units, an additional amount of mineral N was applied in the first year and NPK fertilizers in consecutive years. Mineral fertilizers were applied at the beginning of the growing period. Compared to control, the application of mineral and organic fertilizers resulted in a significant increase in Dactylis glomerata yield. The highest biomass yield (average over the growing periods) was recorded on the unit treated with manure, straw and mineral fertilizers (27.64 g•pot-1) and on the one with mushroom substrate applied together with rye straw and mineral fertilizers (26.47 g•pot-1). The K:(Ca+Mg) ratio in the forage was normal and averaged 0.933, but mineral fertilizers, compared to other treatments, narrowed it.
    Źródło:
    Journal of Ecological Engineering; 2023, 24, 7; 108--118
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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