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Tytuł:
Antibacterial Composite Layers on Ti: Role of ZnO Nanoparticles
Autorzy:
Roguska, A.
Belcarz, A.
Suchecki, P.
Andrzejczuk, M.
Lewandowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/355309.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone implants
TiO2 nanotubes
ZnO nanoparticles
Staphylococcus epidermidis
antibacterial properties
Opis:
Problem of post-operative infections of implant materials caused by bacterial adhesion to their surfaces is very serious. Enhancement of antibacterial properties is potentially beneficial for biomaterials value. Therefore, the metallic and metallic oxide nanoparticles attract particular attention as antimicrobial factors. The aim of this work was to create nanotubular (NT) oxide layers on Ti with the addition of ZnO nanoparticles, designed for antibacterial biomedical coatings. Antimicrobial activities of titanium, TiO2 NT and ZnO/TiO2 NT surfaces were evaluated against bacterial strain typical for orthopaedic infections: S. epidermidis. TiO2 NT alone killed the free bacterial cells significantly but promoted their adhesion to the surfaces. The presence of moderate amount of ZnO nanoparticles significantly reduced the S. epidermidis cells adhesion and viability of bacterial cells in contact with modified surfaces. However, higher amount of loaded nanoZnO showed the reduced antimicrobial properties than the medium amount, suggesting the overdose effect.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 2B; 937-940
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial Composite Layers on Ti: Role of ZnO Nanoparticles
Autorzy:
Roguska, A.
Belcarz, A.
Suchecki, P.
Andrzejczuk, M.
Lewandowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/354849.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone implants
TiO2 nanotubes
ZnO nanoparticles
Staphylococcus epidermidis
antibacterial properties
Opis:
Problem of Post-operative infections of implant materials caused by bacterial adhesion to their surfaces is very serious. Enhancement of antibacterial properties is potentially beneficial for biomaterials value. Therefore, the metallic and metallic oxide nanoparticles attract particular attention as antimicrobial factors. The aim of this work was to create nanotubular (NT) oxide layers on Ti with the addition of ZnO nanoparticles, designed for antibacterial biomedical coatings. Antimicrobial activities of titanium, TiO2 NT and ZnO/TiO2 NT surfaces were evaluated against bacterial strain typical for orthopaedic infections: S. epidermidis. TiO2 NT alone killed the free bacterial cells significantly but promoted their adhesion to the surfaces. The presence of moderate amount of ZnO nanoparticles significantly reduced the S. epidermidis cells adhesion and viability of bacterial cells in contact with modified surfaces. However, higher amount of loaded nanoZnO showed the reduced antimicrobial properties than the medium amount, suggesting the overdose effect.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 1; 213-216
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of electrochemical impedance spectroscopy in analysis of SiO2 layer used for implants in bone surgery
Autorzy:
Walke, W.
Hyla, A.
Marciniak, J.
Nowińska, K.
Powiązania:
https://bibliotekanauki.pl/articles/284466.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
implants
bone surgery
morphological structure
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 113
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioactivity of cement type bone substitutes
Autorzy:
Siek, D.
Czechowska, J.
Mróz, W.
Zima, A.
Burdyńska, S.
Załęczny, R.
Ślósarczyk, A.
Powiązania:
https://bibliotekanauki.pl/articles/200610.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone implants
bioactivity
surface morphology
Opis:
In vitro chemical stability and bioactivity of three different cement type bone substitutes were determined by incubating cement samples in the simulated body fluid (SBF) for 7 and 28 days. Morphology of sample surfaces has been studied using scanning electron microscopy (SEM) combined with an energy dispersive X-ray spectroscopy (EDS) and by atomic force microscopy (AFM). The diffuse reflectance Fourier-transform infrared spectroscopy (DRIFTS) was applied as a supplementary method. The development of bone-like apatite layers on the surface depended on their initial phase composition. Obtained cements showed good surgical handiness, high bioactive potential and were chemically stable. They seem to be promising materials for bone substitution.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2013, 61, 2; 433-439
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioszkła i organiczno-nieorganiczne kompozyty dla inżynierii tkankowej kości
Bioglasses and organic-inorganic composites for bone tissue engineering
Autorzy:
John, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/172492.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
biomateriały
bioszkło
organiczno-nieorganiczne hybrydy
hydroksyapatyt
implanty kości
in vitro
biomaterials
bioglasses
organic-inorganic hybrid
hydroxyapatite
bone implants
Opis:
The most demanded biomaterials for bone tissue engineering could be classified in two main sol-gel derived groups: bioglasses and organic-inorganic composites. The first of these include bioactive ceramics such as calcium phosphates (Tab. 2) [1], glasses and glass ceramics [2], and so-called inert ceramics (Tab. 1) such as Al2O3, zirconium and titanium dioxide, and carbon-based materials [3, 4]. Second-group of compounds constitute bioactive organic-inorganic hybrids, generally based on organic matrix and various inorganic dopants. Biomaterials in contact with human plasma and bone stem cells form, on their surface, hydroxyapatite Ca10(PO4)6(OH)2 (HAp) and its derivatives (Tab. 2). HAp-layer initiates bone growth and reconstruction of treated fragment (Fig. 2). These materials, due to the high degree of biocompability are considered as the most valuable compounds for bone surgery [5]. Extremely rapid development of biomaterials used in medicine caused the production of implants with different properties (Scheme 1). The real revolution and technological progress have brought biomimetic composites that mimic naturally occurring solutions in living organisms. The role of such implants is not only replacing the damaged parts of body, but – due to the appropriate morphology and composition – stimulating the growth of living cells (Fig. 3) and final bone regeneration. This article is devoted to this type of biomaterials proposed for bone tissue engineering.
Źródło:
Wiadomości Chemiczne; 2012, 66, 1-2; 21-39
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cement i implanty szkło-jonomerowe do stosowania w otochirurgii
Glass-ionomer cement and implants for use in otosurgery
Autorzy:
Skarżyński, H.
Karaś, J.
Floriańczyk, T.
Powiązania:
https://bibliotekanauki.pl/articles/167969.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Ceramiki i Materiałów Budowlanych
Tematy:
otochirurgia
niedosłuch
ubytki kostne
rekonstrukcje
cement szkło-jonomerowy
implanty szkło-jonomerowe
badania kliniczne
otosurgery
hypoacusia
bone defects
reconstructions
glass-ionomer cement
glass-ionomer implants
clinical studies
Opis:
W artykule przedstawiono cement i implanty szkło-jonomerowe, do stosowania w otochirurgii, opracowane i wytworzone w Instytucie Ceramiki i Materiałów Budowlanych. Leczenie niedosłuchu wymaga rekonstrukcji ubytków w łańcuchu kosteczek słuchowych w uchu środkowym w przypadkach patologicznych spowodowanych przewlekłymi stanami zapalnymi, urazem lub wadami wrodzonymi. Kliniczne badania zostały przeprowadzone w Instytucie Fizjologii i Patologii Słuchu w Kajetanach. W artykule zaprezentowano wyniki tych długoterminowych badań. Zastosowanie materiałów szkło-jonomerowych jest najlepszym sposobem trwałego odtworzenia uszkodzonego układu przewodzącego w uchu środkowym, umożliwia szeroki zakres anatomicznych rekonstrukcji ubytków kostnych będących rezultatem procesów chorobowych lub usunięcia chirurgicznego. Anatomiczne rekonstrukcje pozwalają odzyskać pierwotną i optymalną funkcję leczonych struktur. Zastosowanie implantów szkło-jonomerowych znacząco skraca czas operacji. W jednakowym stopniu są one przydatne w leczeniu otochirurgicznym dzieci i dorosłych.
In this paper glass-ionomer cement and implants for use in otosurgery, which were developed and produced at the Institute of Ceramics and Building Materials, were presented. Treatment of hypoacusia requires defects to be reconstructed in the ossicular chain of middle ear in the cases of pathology resulting from chronic ear inflammations, trauma or congenital malformations. The clinical studies have been performed at the Institute of Physiology and Pathology of Hearing in Kajetany. Results of these long-term studies were presented. The application of glass-ionomer materials is the best method giving excellent results with regard to the reconstruction of a damaged conducting system in the middle ear. The use of cement and implants allows the wide range of anatomical reconstructions of bone defects that are result of disease processes or surgical removal. The anatomic reconstructions allow to restore primary and optimal function of the treated tissues. Application of glass-ionomer implants enables significant reduction in operation time. The glass-ionomer cement and implants are equally useful for reconstructive surgery in children and adults.
Źródło:
Szkło i Ceramika; 2013, R. 64, nr 1, 1; 1-7
0039-8144
Pojawia się w:
Szkło i Ceramika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of environmental parameters on the concentration of nickel (Ni) in bones of the hip joint from patients with osteoarthritis
Autorzy:
Łanocha-Arendarczyk, Natalia
Kalisińska, Elżbieta
Kosik-Bogacka, Danuta
Budis, Halina
Lewicka, Katarzyna
Sokołowski, Sebastian
Dobiecki, Konrad
Kołodziej, Łukasz
Powiązania:
https://bibliotekanauki.pl/articles/972587.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
bone
nickel
environmental exposure
implants
Opis:
Introduction. Bone trace elements levels including nickel (Ni) depend on biological and environmental factors: age, gender, remodeling state, exposure to occupational and environmental pollution, smoking, diet, and surgical implants. Objective. The aim of this study was to determine Ni concentrations in bones of femur heads obtained from patients with osteoarthritis. Materials and method. A total of 111 samples of hip joint bones (cartilage, cancellous bone and compact bone) collected from patients from north-western Poland were examined. Ni concentration was determined by ICP-AES (atomic absorption spectrophotometry). Results. It was found that differences in Ni concentrations were statistically significant between cartilage and compact bone. Cartilage Ni concentrations were higher in patients with fractured femur neck than patients with osteoarthritis. Furthermore, higher Ni levels were also found in samples obtained from smokers compared to nonsmokers. In cancellous bone, higher Ni concentrations were found in samples from patients who had received implants than those without them. Moreover, higher Ni concentrations in cancellous bone were found in patients occupationally exposed to heavy metals than in those not exposed. Conclusions. The bone tissue reflects long-term exposure to Ni and may be used as a bioindicator to study the process of Ni accumulation in the human body.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2016, 10, 1; 6-11
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kompozyt polimerowo-ceramiczny jako potencjalny implant kostny
Polymer-ceramic composite as a potential bone implant
Autorzy:
Jelonek, A.
Kilan, K.
Skórska-Stania, A.
Oleksyn, B.
Powiązania:
https://bibliotekanauki.pl/articles/284768.pdf
Data publikacji:
2010
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
composites
bone implants
polymers
kompozyty
implanty kostne
polimery
Źródło:
Engineering of Biomaterials; 2010, 13, no. 99-101; 125-127
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Marginal bone loss around dental implants with various types of implant-abutment connection in the same patient
Autorzy:
Szymańska, Jolanta
Szpak, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/972731.pdf
Data publikacji:
2017
Wydawca:
Instytut Medycyny Wsi
Tematy:
dental implants
abutment type
marginal bone loss
humans
Opis:
Introduction. One of the criteria of implant therapy success is marginal bone loss. The objective of the study was to assess the correlation between peri-implant marginal bone loss and implant-abutment connection systems used in the same patient, as well as other specific characteristics of implant treatment. The initial research hypothesis assumed that there was no difference in marginal bone loss around implants with different implant-abutment connection systems placed in the same patient. Materials and method. Marginal bone loss was assessed around implants with two different types of implant-abutment connection: with conical (Type I) and with internal hexagonal (Type II) in the same patient. The study included 28 patients aged 37–66 years. Results. Marginal bone loss around Type I implants was 0.112 mm/month before loading with prosthetic restorations, and 0.010 mm/month after loading, while for Type II implants it reached, respectively, 0.123 mm/month and 0.030 mm/month. Marginal bone loss after loading with prosthetic restorations was 11 times lower for Type I implants and 4 times lower for Type II implants. Evaluation of marginal bone loss in the studied patient groups was made on the basis of orthopantomographic radiographs. Conclusions. Implants with conical implant-abutment connection are significantly more favourable to osseointegration than those with internal hexagonal connection. As marginal bone loss is faster before loading implants with prosthetic restorations than after loading, it is advisable to consider early loading if the necessary clinical conditions are met.
Źródło:
Journal of Pre-Clinical and Clinical Research; 2017, 11, 1
1898-2395
Pojawia się w:
Journal of Pre-Clinical and Clinical Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
NiTi shape memory clamps for bone fracture treatment in rabbit: preliminary report
Autorzy:
Goryczka, T.
Lekston, Z.
Lelątko, J.
Szponder, T.
Paluch, J.
Powiązania:
https://bibliotekanauki.pl/articles/286018.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
shape memory alloy
bone fracture
orthopedic implants
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 72
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena tomograficzna kontaktu implantu tytanowego z tkanką kostną świńskiego kręgosłupa
Tomographical evaluation of the contact of titanium implant with bone tissue of the porcine spine
Autorzy:
Ciupik, L. F.
Flis, E.
Powiązania:
https://bibliotekanauki.pl/articles/283775.pdf
Data publikacji:
2011
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
implant kręgosłupowy
tomografia komputerowa
preparat świński
kontakt implant-tkanka kostna
spinal implants
computed tomography
porcine specimen
implant-bone tissue contact
Opis:
Główną funkcją implantów jest dążność do odtworzenia i utrzymania proporcji między elementami struktury układu kostno-mięśniowego. Blaszka graniczna trzonu jest elementem przenoszącym obciążenia biomechaniczne. W badaniach na preparatach świńskich z użyciem tomografu komputerowego 3D zobrazowano geometrię kontaktu tytanowego implantu gwintowanego w przestrzeni międzykręgowej.
The main function of implants is their tendency to restoration and maintenance of proportions between elements of the skeleto-muscular system. A vertebral endplate is the element, which transfers biomechanical loads. During studies on porcine specimens using computed tomograph 3D, geometry of a contact of titanium threaded implant in an inter-vertebral space has been visualized.
Źródło:
Engineering of Biomaterials; 2011, 14, no. 106-108; 95-98
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena zagrożeń wynikających z oddziaływania pola magnetycznego emitowanego przez aplikator magnetoterapeutyczny dla użytkowników protez słuchu wykorzystujących przewodnictwo kostne
Evaluation of hazards caused by magnetic field emitted from magnetotherapy applicator to the users of bone conduction hearing prostheses
Autorzy:
Zradziński, Patryk
Karpowicz, Jolanta
Gryz, Krzysztof
Leszko, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/2164067.pdf
Data publikacji:
2017-06-27
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
symulacje numeryczne
pola elektromagnetyczne
implanty słuchowe
przewodnictwo kostne
indukowane pole elektryczne
Dyrektywa 2013/35/UE
numerical simulations
electromagnetic fields
hearing implants
bone conduction
induced electric field
Directive 2013/35/EU
Opis:
Wstęp Pole magnetyczne małej częstotliwości, wywołując wewnątrz struktur elektrycznie przewodzących indukowane pole elektryczne (Ein), może bezpośrednio oddziaływać na funkcjonowanie organizmu, np. poprzez elektrostymulację układu nerwowego. Ponadto rozkład przestrzenny i natężenie Ein są zaburzone w sąsiedztwie elektroprzewodzących struktur implantu medycznego. Materiał i metody Opracowano numeryczne modele aplikatora do magnetoterapii, będącego źródłem pola magnetycznego sinusoidalnie zmiennego o częstotliwości 100 Hz, oraz użytkownika częściowo implantowanej protezy słuchu (implantu słuchowego wykorzystującego przewodnictwo kostne: typu Bonebridge (IS-BB) lub implantu słuchowego typu BAHA (IS-BAHA – bone anchored hearing aid, implant słuchu zakotwiczony w kości). Przeanalizowano wartości Ein w modelu głowy użytkownika implantu przebywającego obok aplikatora (np. fizjoterapeuty). Wyniki Wykazano, że używanie IS-BB lub IS-BAHA istotnie zwiększa (do ok. 4-krotnie) zagrożenia elektromagnetyczne jego użytkownika w porównaniu z osobą bez implantu, narażoną na niejednorodne przestrzennie pole magnetyczne. Zagrożenie dla użytkownika IS-BAHA jest większe niż użytkownika IS-BB. Stwierdzono, że przy zastosowaniu zasad oceny określonych w Dyrektywie 2013/35/UE w przypadku użytkowników implantów słuchowych przy narażeniu na pole niejednorodne słabsze od limitów indukcji magnetycznej może wystąpić przekroczenie limitów natężenia pola indukowanego w organizmie. Natomiast w przypadku stosowania wymagań i limitów określonych w polskim prawie pracy lub zaleceniach ICNIRP (International Commission on Non-Ionizing Radiation Protection – Międzynarodowa Komisja ds. Ochrony Przed Promieniowaniem Niejonizującym) dotrzymanie wymagań dotyczących poziomu ekspozycji zapewnia również dotrzymanie wymagań dotyczących odpowiednich limitów pola indukowanego w organizmie użytkownika implantu słuchowego. Wnioski Konieczne jest wykonanie indywidualnej oceny zagrożeń elektromagnetycznych dotyczących użytkowników implantów słuchowych ze względu na stwierdzone istotnie większe zagrożenia w stosunku do osób bez implantu oraz różnic w poziomie zagrożenia użytkowników implantów o odmiennych rozwiązaniach konstrukcyjnych czy technologicznych. Med. Pr. 2017;68(4):469–477
Background Low frequency magnetic field, inducing electrical field (Ein) inside conductive structures may directly affect the human body, e.g., by electrostimulation in the nervous system. In addition, the spatial distribution and level of Ein are disturbed in tissues neighbouring the medical implant. Material and Methods Numerical models of magneto-therapeutic applicator (emitting sinusoidal magnetic field of frequency 100 Hz) and the user of hearing implant (based on bone conduction: Bonebridge type – IS-BB or BAHA (bone anchorde hearing aid) type – IS-BAHA) were worked out. Values of Ein were analyzed in the model of the implant user’s head, e.g., physiotherapist, placed next to the applicator. Results It was demonstrated that the use of IS-BB or IS-BAHA makes electromagnetic hazards significantly higher (up to 4-fold) compared to the person without implant exposed to magnetic field heterogeneous in space. Hazards for IS-BAHA users are higher than those for IS-BB users. It was found that applying the principles of directive 2013/35/EU, at exposure to magnetic field below exposure limits the direct biophysical effects of exposure in hearing prosthesis users may exceed relevant limits. Whereas applying principles and limits set up by Polish labor law or the International Commission on Non-Ionizing Radiation Protection (ICNIRP) guidelines, the compliance with the exposure limits also ensures the compliance with relevant limits of electric field induced in the body of hearing implant user. Conclusions It is necessary to assess individually electromagnetic hazard concerning hearing implant users bearing in mind significantly higher hazards to them compared to person without implant or differences between levels of hazards faced by users of implants of various structural or technological solutions. Med Pr 2017;68(4):469–477
Źródło:
Medycyna Pracy; 2017, 68, 4; 469-477
0465-5893
2353-1339
Pojawia się w:
Medycyna Pracy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Operative treatment of bone fracture with Kirschner wires – young cat case
Autorzy:
Ziąbka, M.
Derkowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/284836.pdf
Data publikacji:
2017
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone fracture
Kirschner wires
implants
Źródło:
Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 46
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Potential activation of the immune system on metallic materials for bone implants
Autorzy:
Stranavova, L.
Bacakova, M.
Novotna, K.
Bacakova, L.
Fencl, J.
Powiązania:
https://bibliotekanauki.pl/articles/285314.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone implants
metallic materials
biomaterials
Opis:
Titanium and stainless steel are metallic materials that have been in use for a long time in orthopedics, traumatology and stomatology. These metals are strong, corrosion-resistant and biocompatible. However, metallic materials have some disadvantages in comparison with the natural bone, particularly their relatively high specific weight and toughness. For example, the Young's modulus of AISI316L stainless steel, Co-Cr alloys and Ti-6Al-4V alloy, i.e. materials frequently used for implantation into bone, ranges between 110-220 GPa, while the Young's modulus of bone tissue is 10-40 GPa [1]. In addition, these metals can release cytotoxic, allergenic and immunogenic ions, which can affect their biocompatibility [2, 3]. Implantation is a special type of transplantation process, in which the implant is inserted into the body, usually in order to replace an irreversibly damaged tissue. However, the immune system recognizes the implant as a foreign substance and attacks it with its effector mechanisms. Just as it can reject other types of transplants, the immune system can reject an artificial implant. To prevent rejection of an implant, it is important to study the potential activation of the immune system. This study has investigated the biocompatibility of samples made of pure titanium (according to quality standard ISO 5832-2) and corrosion-resistant steel (quality standards ISO 5832-1 and AISI 316L), obtained from Beznoska Ltd. (Kladno, Czech Republic), and the potential activation of the immune system by these materials. In addition to Fe, the steel samples contained C (max. 0.025 wt.%), Si (0.6 wt.%), Mn (1.7 wt.%), P (max. 0.025wt.%), S (max. 0.003 wt.%), Cr (17.5 wt.%), Ni (13.5 wt.%), Mo (2.8 wt.%), and Cu (max. 0.1 wt. %). The materials were used in the form of square samples (9x9 mm or 30x30 mm, thick¬ness 1 mm). Both the Ti samples and the steel samples were ground with SiO2. The surface of the steel samples was then treated by polishing with Al2O3 paste (grain size up to 1 um), while the surface of the Ti samples, i.e. a material not suitable for polishing, was finished by brushing using another type of Al2O3 paste with slightly larger grains. Thus, the surface of the steel samples was finally smoother and glossy, while the Ti surface was rougher and matte. For the in vitro biocompatibility tests, human osteoblast-like MG 63 cells (European Collection of Cell Cultures, Salisbury, UK) were used. The smaller samples (9x9 mm) were inserted into polystyrene 24-well cell culture plates (TPP, Trasadingen, Switzerland; well diameter 1.5 cm). Each well contained 25 000 cells (approx. 14 150 cells/cm2) and 1.5 ml of Dulbecco's Modified Eagle Minimum Essential Medium (DMEM; Sigma, USA, Cat. No. 10270-106) supplemented with 10% foetal bovine serum (FBS; Gibco, Cat. No. 10270-106) and gentamicin (40 /jg/ml, LEK, Slovenia). These samples were used for evaluating the size of the cell spreading area (day 1), and for evaluating cell shape and cell viability (days 1, 4 and 7 after seeding). The size of the cell spreading area was measured using Atlas Software (Tescan Ltd., Brno, Czech Republic). The viability of the cells was determined by the LIVE/ DEAD viability/cytotoxicity kit for mammalian cells (Invitrogen, Molecular Probes, USA). The larger samples (30x30 mm) were inserted into GAMA polystyrene dishes (diameter 5 cm; GAMA Group Joint-Stock Company, Ceske Budejovice, Czech Republic) and seeded with 300 000 cells/dish (approx. 15 300 cells/cm2) suspended in 9 ml of the above mentioned culture medium. These samples were used for evaluating the cell number on days 1, 4 and 7 after seeding, using a Beckman Vi-CELL XR Cell Analyser automatic cell counter. For the in vitro analysis of markers of osteogenic differentiation and cell immune activation, human osteoblast-like MG 63 cells (European Collection of Cell Cultures, Salisbury, UK) were used. The samples (9x9 mm) were inserted into polystyrene 24-well cell culture plates (TPP, Trasadingen, Switzerland; well diameter 1.5 cm). Each well contained 25 000 cells (approx. 14 150 cells/cm2) and 1.5 ml of Dulbecco's Modified Eagle Minimum Essential Medium (DMEM; Sigma, USA, Cat. No. 10270-106) supplemented with 10% foetal bovine serum (FBS; Gibco, Cat. No. 10270-106) and gentamicin (40 jg/ml, LEK, Slovenia). The cells were cultured for 1, 4, or 7 days at 37°C in a humidified atmosphere of 5% of CO2 in the air. On day 4 after seeding, the medium was changed; one half of the samples contained standard medium DMEM with 10% foetal bovine serum and gentamicin (40 jg/ml) mentioned above, and the second half contained osteogenic medium, i.e. the standard medium further supplemented with ß-glycerophosphate, L-glutamin, ascorbic acid, dihydroxyvitamin D3, dexamethason, 10% foetal bovine serum and gentamicin (40 jg/ml). Using an Enzyme-Linked ImmunoSorbent Assay (ELISA), we measured the concentration of the Inter¬cellular Adhesion Molecule-1 (ICAM-1, a marker of cell immune activation) and osteocalcin (a marker of osteogenic cell differentiation). These measurements were performed in homogenates of cells on days 4 and 7 after seeding, and the concentration of both markers was measured per cell or per mg of protein. On day 7, the amount of osteocalcin was measured and compared in cells cultured in the standard and osteogenic media. We also measured TNF-а and IL- 1ß, i.e. other markers of cell immune activation. These cytokines are important mediators of the inflammatory response, and they are involved in a variety of cellular activities, including cell proliferation and differentiation. We measured the secretion of these markers into the cell culture medium in murine macrophage-like RAW264.7 cells (American Type Culture Collection, Manassas, VA). The samples (9x9 mm) were inserted into polystyrene 24-well cell culture plates (TPP, Tra- sadingen, Switzerland; well diameter 1.5 cm). Each well contained 30,000 (approx. 16 980 cells/cm2) cells and 1.5 ml of the culture medium. RAW 264.7 cells were cultured in the RPMI-1640 medium (Sigma; 10% fetal bovine serum, 40 jg/mL gentamicin). After 7 days of cultivation, the cell culture medium was collected and used for measuring the concentration of TNF-а and IL-1ß by a sandwich ELISA using commercially available kits. A mouse TNF-а kit and an IL- 1ß Quantikine ELISA kit were used for the RAW 264.7 cells. Both kits were purchased from R and D Systems (Minneapolis, MN) and used according to the manufacturer's protocol. The results indicated that the number of initially adhering MG 63 cells on day 1 after seeding was significantly lower on the titanium (5320±390 cells/cm2) and on the stainless steel (4110±370 cells/cm2) than on the control polystyrene culture dishes (7740±350 cells/cm2). However, on day 4 after seeding, the cell population density on both metallic materials became significantly higher than on the control polystyrene dishes (75200±2890 cells/cm2 on Ti and 90 870±2350 cells/cm2 on steel vs. 56440±1180 cells/cm2 on polystyrene). This suggests faster cell proliferation on both metallic materials than on polystyrene. At the same time, the cell number on the stainless steel samples was significantly higher than on the Ti samples. On day 7, the differences in the number of adhered cells on the two metals and on the control polystyrene substrate was on an average similar (from 328780±680 cells/cm2 to 362200±760 cells/cm2). The cell viability on all tested materials was almost 100% in all culture intervals. The morphology of the cells adhered on the studied materials was similar to the morphology of the cells on the control polystyrene dishes, i.e. the cells were mostly flat and polygonal, and the size of their cell spreading areas was similar on all tested materials. The cells were distributed homogeneously on the entire material surface, and on day 4 they started to form confluent cell layers. On day 4, we measured the amount of ICAM-1 by the ELISA test. This immunoglobulin molecule is typically expressed on cells of the immune system, but it is also expressed on other cell types, including MG 63, during their immune activation, e.g. by an artificial growth support. In this case, ICAM-1 molecules on cells are bound byß2-integrin receptors on inflammatory cells (for a review, see [4]). Surprisingly, titanium seemed to be more immunogenic than stainless steel, which was indicated by a higher concentration of ICAM-1 per cell and mg of protein in cells on day 4 after seeding. However, on day 7, there was no difference between the concentrations of ICAM-1 per cell and mg of protein in cells on titanium and on stainless steel. The second molecule that we measured was osteocalcin, a calcium-binding extracellular matrix glycoprotein, an important marker of the bone formation process. The concentration of osteocalcin on day 4 in the standard culture medium was higher in MG 63 cells on the titanium and stainless steel than on the control polystyrene samples. This could be explained by the fact that the metals are harder than polystyrene. It is known that harder substrates promote osteogenic cell differentiation, while softer substrates direct the cell differentiation towards neural or muscle phenotype [5]. In addition, the osteogenic differentiation was further supported by the osteogenic medium, as indicated by a higher concentration of osteocalcin in cells grown in this medium compared to cells in the standard medium on day 7 after seeding. On day 7 after seeding murine macrophage-like RAW264.7 cells on the tested materials, the concentration of TNF-а in the culture medium ranged on an average from 57.10 to 79.39 pg per 2000000 cells. The concentration of TNF-а in the medium from Ti and Fe was significantly higher than in the medium from the control polystyrene dishes. The highest value (79.39 pg/2000000 cells) was found in the medium taken from RAW264.7 cells on Ti. The second molecule that we tested was IL-1ß. No significant differences in the concentration of IL-1ß were detected in the culture medium obtained from RAW264.7 cells on all tested materials. In other words, neither type of metallic material, i.e. Ti and Fe, evoked significantly higher production of IL-1ß by RAW 264.7 cells than standard polystyrene cell culture dishes. It can be concluded that the tests of biocompatibility and immune activation confirmed that titanium and stainless are promising for construction of bone implants and for good integration with the surrounding bone tissue.
Źródło:
Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 130-131
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Recording of basal calcium levels in cells on geometric structures
Autorzy:
Staehlke, S.
Moerke, C.
Nebe, J. B.
Powiązania:
https://bibliotekanauki.pl/articles/285500.pdf
Data publikacji:
2016
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
implants
tissue engineering
bone tissue
Źródło:
Engineering of Biomaterials; 2016, 19, 138; 42
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 20

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