- Tytuł:
- Morphology and developmental potential of bovine parthenotes after spontaneous activation in vitro
- Autorzy:
-
Lechniak, D
Cieslak, D.
Sosnowski, J. - Powiązania:
- https://bibliotekanauki.pl/articles/2048294.pdf
- Data publikacji:
- 1998
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
blastomere
bovine oocyte
cytogenetic analysis
parthenogenetic activation
morphology
in vitro
fertilization system
blastocyst
oocyte
insemination
morphological evaluation
maturation
embryo production - Opis:
- Spontaneous parthenogenetic activation of bovine oocytes in an in vitro maturation and fertilization system (IVM/IVF) is described. Altogether, 1403 follicular oocytes, collected by the aspiration method, were matured in vitro and then cultured without insemination in the same conditions as a group of inseminated oocytes. After 48-72 h of additional culture, 141 oocytes (10%) were found to be spontaneously activated. Morphological evaluation revealed that the number of blastomeres within parthenotes ranged from 2 to 16 cells, with a minority (15.7%) comprising of 9-16 blastomeres. According to a cytogenetic analysis, only 1.2% of the analysed parthenotes consisted of more than 9 cells. Parthenotes may not be distinguished from embryos produced in vitro and spontaneous parthenogenetic activation in an IVM/IVF system indicates suboptimal culture conditions. A group of non-inseminated oocytes should be included in each experiment to serve as a control. Spontaneously activated bovine parthenotes only occasionaly developed beyond the 8-blastomere stage in a common IVM/IVF system. The incidence of parthenotes interferes with the efficiency of in vitro embryo production but it is doubtful whether it lowers the pregnancy rate after transfer of IVF embryos.
- Źródło:
-
Journal of Applied Genetics; 1998, 39, 2; 193-198
1234-1983 - Pojawia się w:
- Journal of Applied Genetics
- Dostawca treści:
- Biblioteka Nauki
Artykuł