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Wyszukujesz frazę "bacterial exopolysaccharides" wg kryterium: Temat

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    Wyświetlanie 1-3 z 3
    Tytuł:
    In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
    Autorzy:
    Komorowski, Piotr
    Kołodziejczyk, Agnieszka
    Makowski, Krzysztof
    Kotarba, Sylwia
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1844871.pdf
    Data publikacji:
    2021
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bacterial exopolysaccharides
    dextran- -based “microcarriers”
    scanning electron microscopy
    atomic force microscopy
    roughness parameters
    three-dimensional cell culture
    Opis:
    The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
    Źródło:
    Engineering of Biomaterials; 2021, 24, 159; 18-23
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ultrasound conditioning of Streptococcus thermophilus CNRZ 447: growth, biofilm formation, exopolysaccharide production, and cell membrane permeability
    Autorzy:
    Khadem, H.
    Tirtouil, A.M.
    Drabo, M.S.
    Boubakeur, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096999.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Streptococcus thermophilus
    ultrasound conditioning
    bacterial adhesion
    biofilm
    exopolysaccharides
    Opis:
    Sonication is one of the new and innovative approaches that is being increasingly used in food industry to control fermentation processes and to eradicate spoiling. Recently, this approach has seen new industrial applications such as enhancing microbial productivity. The present study aimed to assess the effects of ultrasound conditioning on the metabolism and extracellular matrix production of Streptococcus thermophilus. Bacterial suspensions were treated in ultrasonic bath (35 kHz, 240/60 W peak/nominal power, 1.8 l capacity) for different time periods (5, 10, 15, 20, 30, 45, and 65 min), and the growth improvement, adhesion ability, biofilm formation, and exopolysaccharide production of the bacterial strain were measured. The bacterial strain exhibited resistance to the treatment, and the conditioning improved the growth, adhesion, membrane permeability, biofilm formation, and exopolysaccharide production ability. An optimal treatment was obtained for 30 minutes of conditioning. An excellent yield of desirable exopolysaccharides (1788 mg glucose equivalent/l) was achieved. Ultrasound conditioning may be used as a potential approach to enhance certain biotechnological properties of industrial microorganisms.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 159-165
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Egzopolisacharydy bakteryjne : budowa i funkcje
    Bacterial exopolysaccharides : structure and functions
    Autorzy:
    Samaszko-Fiertek, J.
    Kuźma, M.
    Dmochowska, B.
    Ślusarz, R.
    Madaj, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/172244.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    egzopolisacharydy
    kwas hialuronowy
    celuloza bakteryjna
    dekstran
    żelan
    ksantan
    exopolysaccharides
    hyaluronic acid
    bacterial cellulose
    dextran
    gellan
    xanthan
    Opis:
    Exopolysaccharides fulfil protective functions and allow bacteria live in the communities, single or mixed, by facilitating adhesion to surfaces and to each other. Microbes prefer to exist in the form of a biofilm. The term biofilm was introduced in 1978 and is the group of microorganisms surrounded by extracellular, highly hydrated mucus, which allows adhesion on various surfaces and adhesion of cells to each other [1]. The extracellular slime owes its character mainly due to the presence of exopolysaccharides. Bacteria living in biofilms, have a high resistance to external factors, such as changes in temperature, pH, humidity, oxygenation, presence of bacteriocins, antibodies or antibiotics. They may be up to 1,000 times more resistant to antibiotics than planktonic forms. They can be synthesized inside and outside bacteria cell. The structure of the bacterial exopolysaccharide is very diverse, but very often, due to the presence of uronic acid residues, or non-sugar organic acids as pyruvic acid, succinic acid, as well as residues of inorganic acids such as phosphoric acid or sulfuric acid, they are negatively charged particles. In addition, a characteristic of most of the exopolysaccharides (EPS) is their enormous molecular mass of up to several million g/mol [11]. Thanks to its rheological properties, ease of isolation, and often biodegradable antioxidant activity extracellular polysaccharides are increasingly used in industry as a gelling agents, hardening and thickening agents, emulsifiers, food coatings and pharmaceutical products. In addition, they can be used as bandages, anti-cancer agents, cholesterol-lowering, antiulcer or immunomodulators [20–27]. This article discusses in details the selected exopolysaccharides such as xanthan, gellan, exopolysaccharides of lactic acid bacteria, dextran, bacterial cellulose, alginic acid, hyaluronic acid, mannans.
    Źródło:
    Wiadomości Chemiczne; 2016, 70, 7-8; 473-496
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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