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    Tytuł:
    Plant expression systems for production of hemagglutinin as a vaccine against influenza virus
    Autorzy:
    Redkiewicz, Patrycja
    Sirko, Agnieszka
    Kamel, Katarzyna
    Góra-Sochacka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039262.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    recombinant hemagglutinin
    avian influenza
    plant-produced
    VLP
    ELISA
    Opis:
    Many examples of a successful application of plant-based expression systems for production of biologically active recombinant proteins exist in the literature. These systems can function as inexpensive platforms for the large scale production of recombinant pharmaceuticals or subunit vaccines. Hemagglutinin (HA) is a major surface antigen of the influenza virus, thus it is in the centre of interests of various subunit vaccine engineering programs. Large scale production of recombinant HA in traditional expression systems, such as mammalian or insect cells, besides other limitations, is expensive and time-consuming. These difficulties stimulate an ever-increasing interest in plant-based production of this recombinant protein. Over the last few years many successful cases of HA production in plants, using both transient and stable expression systems have been reported. Various forms of recombinant HA, including monomers, trimers, virus like particles (VLPs) or chimeric proteins containing its fusion with other polypeptides were obtained and shown to maintain a proper antigenicity. Immunizations of animals (mice, ferrets, rabbits or chickens) with some of these plant-derived hemagglutinin variants were performed, and their effectiveness in induction of immunological response and protection against lethal challenge with influenza virus demonstrated. Plant-produced recombinant subunit vaccines and plant-made VLPs were successfully tested in clinical trials (Phase I and II) that confirmed their tolerance and immunogenicity.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 551-560
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Testing of human specimens for the presence of highly pathogenic zoonotic avian influenza virus A [H5N1] in Poland in 2006-2008 - justified or unnecessary steps?
    Autorzy:
    Romanowska, M
    Nowak, I.
    Brydak, L.B.
    Wojtyla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/50626.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    avian influenza virus type A
    zoonosis
    influenza
    H5N1 subtype
    avian influenza
    disease outbreak
    diagnostic test
    Polska
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 239-247
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Avian influenza in Poland
    Autorzy:
    Śmietanka, Krzysztof
    Minta, Zenon
    Powiązania:
    https://bibliotekanauki.pl/articles/1039245.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    avian influenza
    H5N1 virus
    Opis:
    Poland has experienced four episodes of avian influenza (AI) outbreaks over the past two decades. The first epidemic was caused by a low pathogenicity (LPAIV) H7N7 subtype and occurred in fattening and breeder turkeys in 1995. Two waves of H5N1 high pathogenicity avian influenza (HPAI) took place in 2006 and 2007. In spring 2006, 64 cases of the H5N1 virus were detected, mostly in mute swans. In December 2007, ten outbreaks of H5N1 HPAI were detected in commercial poultry (n=9) and wild birds kept in captivity (n=1). The outbreaks in 2006 and 2007 were caused by genetically similar but clearly distinguishable viruses of the 2.2 clade. In 2013, an H9N2 avian influenza virus was detected in 4 fattening turkey holdings. The virus was low pathogenic and a phylogenetic study has shown a close relatedness to the Eurasian lineage of AIV of the wild bird origin. Neither preventive nor prophylactic vaccinations have ever been used in poultry or other birds. Emergency vaccinations using autogenous vaccine were introduced only to control the H7N7 LPAI outbreaks in 1995. The baseline surveillance for AI in live migratory birds and poultry provides a valuable insight into the ecology of AIV at the wild and domestic bird interface. Passive surveillance is in place of early detection of HPAIV infection in dead or moribund birds.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 453-457
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Electrochemical biosensors for detection of avian influenza virus - current status and future trends
    Autorzy:
    Grabowska, Iwona
    Malecka, Kamila
    Jarocka, Urszula
    Radecki, Jerzy
    Radecka, Hanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039249.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    electrochemical biosensors
    genosensors
    immunosensors
    electrochemical detection
    avian influenza virus
    Opis:
    Electrochemical biosensors have emerged as reliable analytical devices suitable for pathogen detection. Low cost, small sample requirement and possibility of miniaturization justifies their increasing development. Thus, we report in this review on the state of the art of avian influenza virus detection with genosensors and immunosensors working by an electrochemical mode. Their working principles focusing on the physical properties of the transducer, the immobilization chemistry, as well as new trends including incorporation of nanoparticles will be presented. Then, we critically review the detection of avian influenza virus in the complex matrices that use electrochemical biosensors and compare them with traditionally applied methods such as ELISA or Western blot.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 471-478
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Active surveillance in poultry in Poland for avian influenza subtypes H5 and H7
    Autorzy:
    Pikuła, Anna
    Śmietanka, Krzysztof
    Lisowska, Anna
    Minta, Zenon
    Powiązania:
    https://bibliotekanauki.pl/articles/1039246.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    avian influenza virus (AIV)
    poultry
    surveillance
    hemagglutination inhibition test (HI)
    Opis:
    A serological surveillance programme for avian influenza A virus (AIV) subtype H5 and H7 in poultry was implemented in Poland in 2008-2013 with two main objectives: i) to detect subclinical infections or previous exposures to AIV H5 and H7 subtypes and ii) to demonstrate the AI- free status of Poland. During this period, over 45 000 serum samples from 2833 holdings were examined using the hemagglutination inhibition test (HI). The presence of HI antibodies was detected in 8 breeder geese holdings (7 positive for H5 and 1 positive for H7 AIV) and in 1 breeder duck holding (H5-positive), which represented 0.32% of all investigated holdings. All seropositive flocks were examined by real time RT-PCR with negative results, which substantiated the AI-free status of Poland. Positive results detected in clinically healthy poultry kept in an open range system indicate prior infections with low pathogenic AIV originating from the wild-bird reservoir.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 459-463
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression, purification and characterization of glycosylated influenza H5N1 hemagglutinin produced in Pichia pastoris
    Autorzy:
    Kopera, Edyta
    Dwornyk, Angela
    Kosson, Piotr
    Florys, Katarzyna
    Sączyńska, Violetta
    Dębski, Janusz
    Cecuda-Adamczewska, Violetta
    Szewczyk, Bogusław
    Zagórski-Ostoja, Włodzimierz
    Grzelak, Krystyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039268.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Avian influenza
    H5N1
    recombinant hemagglutinin
    Pichia pastoris
    Opis:
    The A/swan/Poland/305-135V08/2006 (H5N1-subtype) hemagglutinin (HA) gene was cloned and expressed in yeast Pichia pastoris (P. pastoris). The HA cDNA lacking the C-terminal transmembrane anchor-coding sequence was fused to an α-factor leader peptide and placed under control of the methanol-inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Two P. pastoris strains: SMD 1168 and KM 71 were used for protein expression. Recombinant HA protein was secreted into the culture medium reaching an approximately 15 mg/L (KM 71 strain). Fusion protein with a His6 tag was purified to homogeneity in one step affinity chromatography. SDS-PAGE and MS/MS analysis indicated that the protein is cleaved into HA1 and HA2 domains linked by a disulfide bond. Analysis of the N-linked glycans revealed that the overexpressed HA is fully glycosylated at the same sites as the native HA in the vaccine strain. Immunological activity of the hemagglutinin protein was tested in mice, where rHA elicited a high immune response.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 597-602
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibody response to DNA vaccine against H5N1 avian influenza virus in broilers immunized according to three schedules
    Autorzy:
    Stachyra, Anna
    Góra-Sochacka, Anna
    Zagórski-Ostoja, Włodzimierz
    Król, Ewelina
    Sirko, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/1039267.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Avian influenza
    H5N1
    DNA vaccine
    broilers
    Opis:
    Broiler type chickens were immunized intramuscularly with a DNA vaccine encoding hemagglutinin (HA) from H5N1 avian influenza virus. The chickens were divided into four groups: control group which was not immunized, a group which obtained only one dose, and two groups which were immunized twice, one group with a boost two weeks after the priming and the other four weeks. Blood samples were collected at several time points and the dynamics of the humoral response to the vaccine was studied. High level of anti-HA antibodies was detected only in the last two groups, that is in chickens immunized according to the prime-boost strategy, regardless of the schedule. An additional interesting observation of this study was detection of the cross-reactivity of an anti-H5 HA positive serum with H5N2 and H1N1 viruses, suggesting that the DNA vaccine tested can induce antibodies of a broad specificity.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 593-596
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zakażenia wirusem grypy typu A u gołębi
    Influenza A virus infections of pigeons
    Autorzy:
    Abolnik, Celia
    Powiązania:
    https://bibliotekanauki.pl/articles/22016002.pdf
    Data publikacji:
    2020
    Wydawca:
    Krajowa Izba Lekarsko-Weterynaryjna
    Tematy:
    ptaki
    wirus influenzy A
    epidemiologia
    gołębie
    choroby zwierząt
    influenza ptaków
    avian influenza
    epidemiology
    pigeons
    Opis:
    Influenza A viruses of all subtypes, in their low pathogenic state, form part of the natural microbiota of water bird species. Four intercontinental transmission waves of Goose/Guangdong H5 lineage viruses swept the globe since 2005, each characterized by epidemics in poultry and contact transmission to humans. A significant shift in ecology of avian influenza had occurred. The risks that columbids pose in the ecology and epidemiology of AI have been thoroughly investigated over the course of several decades. Cumulatively, the studies on free-living pigeons, market pigeons and those experimentally infected with LPAI and HPAI demonstrated, that pigeons were of no epidemiological significance in the transmission and spread of HPAI to humans.
    Źródło:
    Życie Weterynaryjne; 2020, 95, 06; 362-364
    0137-6810
    Pojawia się w:
    Życie Weterynaryjne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ptasia grypa - zagrożenie dla zdrowia człowieka oraz sytuacja epidemiologiczna w Polsce w grudniu 2007 roku
    Avian influenza - a risk to human health and epidemilogical situation in Poland in December 2007
    Autorzy:
    Wrzesień, R.
    Budzińska-Wrzesień, E.
    Jarmuł-Pietraszczyk, J.
    Moskwa, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/126093.pdf
    Data publikacji:
    2010
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    choroby zakaźne zwierząt
    ptasia grypa
    sytuacja epidemiologiczna
    contagious animal diseases
    avian influenza
    epidemiological situation
    Opis:
    Choroby zakaźne zwierząt stanowią duże niebezpieczeństwo nie tylko dla zwierząt, ale także dla ludzi. Jedną z najgroźniejszych chorób jest ptasia grypa. Przyczynia się do tego szybki sposób rozprzestrzeniania się wirusa H5N1, a także możliwość mutacji tego patogenu w formę mającą zdolność przenoszenia się z człowieka na człowieka. Do Polski ptasia grypa powróciła w grudniu 2007 roku. Spowodowała szkody wynoszące ponad 12 milionów złotych.
    Animal infectious diseases pose a great threat not only to animals but also to humans. One of the most dangerous diseases is avian influenza because of the rapid spread of H5N1 virus and a possibility of mutations of this pathogen into a form able to transmit from one person to another. In Poland the avian influenza returned in December 2007. It caused losses calculated at 12 million zlotys. A questionnaire was prepared within this study to present the risk posed by the avian influenza from human perspective.
    Źródło:
    Proceedings of ECOpole; 2010, 4, 2; 523-527
    1898-617X
    2084-4557
    Pojawia się w:
    Proceedings of ECOpole
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) under control of the ptcB promoter in Lactococcus lactis
    Autorzy:
    Szatraj, Katarzyna
    Szczepankowska, Agnieszka
    Sączyńska, Violetta
    Florys, Katarzyna
    Gromadzka, Beata
    Łepek, Krzysztof
    Płucienniczak, Grażyna
    Szewczyk, Bogusław
    Zagórski-Ostoja, Włodzimierz
    Bardowski, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1039270.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Lactococcus lactis
    ptcB promoter
    heterologous gene expression
    avian influenza H5N1
    H5 haemagglutinin
    chicken interleukin-2
    Opis:
    Gram-positive and nonpathogenic lactic acid bacteria (LAB) are considered to be promising candidates for the development of new, safe systems of heterologous protein expression. Recombinant LAB has been shown to induce specific local and systemic immune response against selected pathogens, and could be a good alternative to classical attenuated carriers. The main goal of our study was to express the avian influenza haemagglutinin (H5) and chicken interleukin 2 (chIL-2) in Lactococcus lactis. Results of this study were anticipated to lead to construction of lactococcal strain(s) with potential vaccine properties against the avian influenza A (H5N1) virus. Expression of the cloned H5 gene, its His-tagged variant and chIL-2 gene, under the control of the ptcB gene promoter was attested by RT-PCR on transcriptional level and Western or dot blot analysis on translational level, demonstrating that system can be an attractive solution for production of heterologous proteins. The results of the preliminary animal trial conducted in mice are a promising step toward development of a vaccine against avian bird flu using Lactococcus lactis cells as antigen carriers.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 609-614
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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