Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "antifungal susceptibility" wg kryterium: Temat

  Lista filtrów
Wyrównaj
    Wyświetlanie 1-5 z 5
    Tytuł:
    Genotypic discrimination of Aspergillus fumigatus strain from related species within section fumigati
    Autorzy:
    Giray, Betül
    Kocaöğüt, Elif
    Uçar, Füsun B.
    Haliki-Uztan, Alev
    Powiązania:
    https://bibliotekanauki.pl/articles/989031.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    roda
    βtub
    a. fumigatus
    its-pcr
    antifungal susceptibility testing
    Opis:
    Introduction and objective. The aim was to make an exact diagnosis of 20 strains using molecular biological methods which were isolated from the atmosphere of the inpatient rooms in the Oncology and other departments of the Ege University Medical Faculty Hospital, and identified as Aspergillus fumigatus through phenotypic tests, and to determine their antibiotic susceptibility patterns. Materials and method. It was confirmed that the 20 phenotypically-identified A. fumigatus strains belonged to the section Fumigati after they were tested by the ITS-PCR method. Their sequence analysis was performed and the results sent to the NCBI GenBank, and their accession numbers were obtained. For their exact diagnosis at the species level, the β-tub (β-tubulin) and rodA (RodletA) genes were examined with the multiplex PCR. Anti-fungal susceptibility of the 20 strains was determined according to the M38-A2 standards. Results. As a result of ITS-PCR, it was confirmed that 19 of the 20 strains identified as A. fumigatus through the phenotypic methods belonged to the section Fumigati. However, after the detection of β-tub and rodA genes, all 20 strains were identified as A. fumigatus. Conclusion. Although the results of the phenotypic and molecular biological tests applied to filamentous fungi do not often overlap, in this study, the results obtained from the molecular analysis confirmed the results of the phenotypic tests. However, 1 of the 20 strains phenotypically-identified as A. fumigatus was identified as Penicillium spp. as a result of ITSPCR and sequence analysis. On the other hand, the profile obtained from β-tub and rodA tests indicated that the strain was A. fumigatus. Based on these results, this strain is thought to belong to the Aspergilloides genus which has the features of both genera.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2016, 23, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro antifungal susceptibility testing of Scopulariopsis brevicaulis strains using agar diffusion method
    Ocena in vitro lekowrażliwości szczepów Scopulariopsis brevicaulis metodą dyfuzji w agarze
    Autorzy:
    Skora, M.
    Macura, A.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/841599.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro
    antifungal susceptibility
    testing
    Scopulariopsis brevicaulis
    agar diffusion test
    human infection
    Opis:
    The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VOR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered e.g., combined therapy and/or surgery.
    Źródło:
    Annals of Parasitology; 2011, 57, 2
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro antifungal susceptibility testing of Scopulariopsis brevicaulis strains using agar diffusion method
    Ocena in vitro lekowrażliwości szczepów Scopulariopsis brevicaulis metodą dyfuzji w agarze
    Autorzy:
    Skóra, M.
    Macura, A.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2143334.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    in vitro
    antifungal susceptibility
    testing
    Scopulariopsis brevicaulis
    agar diffusion test
    human infection
    Opis:
    The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VOR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered e.g., combined therapy and/or surgery.
    Źródło:
    Wiadomości Parazytologiczne; 2011, 57, 2; 111-117
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of in vitro antifungal activity of preparation "fin Candimis" against Candida strains
    Ocena in vitro aktywności przeciwgrzybiczej preparatu "fin Candimis" wobec szczepów grzybów z rodzaju Candida
    Autorzy:
    Glowacka, A.
    Bednarek-Gejo, A.
    Budak, A.
    Trojanowska, D.
    Mianowany, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/67323.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    assessment
    in vitro
    antifungal activity
    fin Candimis preparation
    Candida albicans
    Candida glabrata
    oregano
    essential oil
    susceptibility
    Opis:
    The aim of the study was to assess the antifungal activity of preparation „fin Candimis” (oregano essential oil) against yeast-like strains belonging to the genus Candida. During the investigation, there were used up nine Candida albicans strains and ten C. glabrata strains isolated from different clinical material, along with one C. albicans demonstration strain ATCC 90028. The oregano essential oil, utilized in the study, was obtained from fresh leaves of Origanum vulgare L. and bore a trade name „fin Candimis”. According to data yielded by its manufacturer, concentration of pure oregano essential oil in preparation „fin Candimis” totals up to 210 mg/ml. The susceptibility of the Candida strains to preparation „fin Candimis” was assessed by means of the disc-diffusion method, upon the Sabouraud solid medium (after a 24-hour incubation of the cultures at temperature of 37 degrees centigrade); the oregano essential oil had been diluted in 1 ml of DMSO, according to the geometrical progression. A measure of the antifungal activity of preparation „fin Candimis” was the minimal inhibitory concentration (MIC), in terms of the fungus growth. Preparation „fin Candimis” is capable of being applied in the prevention and treatment of candidiasis – alone, or as a natural adjunctive agent. The C. albicans strains are more susceptible to preparation „fin Candimis” in comparison to the C. glabrata ones.
    Celem pracy była ocena aktywności przeciwgrzybiczej preparatu „fin Candimis” (olejku eterycznego oregano) wobec szczepów grzybów z rodzaju Candida. Do badań wykorzystano 9 szczepów Candida albicans oraz 10 szczepów Candida glabrata, które wyizolowano z różnych materiałów klinicznych, i 1 szczep wzorcowy Candida albicans ATCC 90028 oraz preparat „fin Candimis”, zawierający olejek eteryczny otrzymany ze świeżych liści oregano. Według danych producenta, stężenie czystego olejku eterycznego oregano w produkcie „fin Candimis” wynosi 210 mg/ml. Wrażliwość szczepów z rodzaju Candida na preparat „fin Candimis” oznaczano metodą dyfuzyjno-krążkową na podłożu Sabouraud’a (po 24-godzinnej inkubacji hodowli w temperaturze 37°C); olejek eteryczny oregano rozcieńczano w 1 ml DMSO według postępu geometrycznego. Miarą przeciwgrzybiczego działania preparatu „fin Candimis” było najmniejsze stężenie hamujące wzrost grzyba (MIC). Preparat „fin Candimis” (olejek eteryczny oregano) może mieć zastosowanie w profilaktyce i leczeniu kandydoz – samodzielnie lub jako naturalny środek wspomagający. Szczepy Candida albicans są bardziej wrażliwe niż szczepy Candida glabrata na preparat „fin Candimis”.
    Źródło:
    Acta Mycologica; 2012, 47, 1
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Susceptibility testing of Aspergillus niger strains isolated from poultry to antifungal drugs - a comparative study of the disk diffusion, broth microdilution (M 38-A) and Etest methods
    Autorzy:
    Tokarzewski, S.
    Ziolkowska, G.
    Nowakiewicz, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/32075.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    susceptibility
    Aspergillus niger
    isolation
    poultry
    antifungal drug
    disk diffusion
    broth microdilution method
    disc-diffusion method
    Etest procedure
    aspergillosis
    fungal infection
    respiratory tract
    Opis:
    The aim of this study was to determine the sensitivity of Aspergillus niger strains isolated from birds to available antifungal drugs using different in vitro assays - classical disk diffusion, Etest® and broth microdilution NCCLS/CLSI M 38-A. The study material consisted of about 2.000 swabs and samples from different species of birds. A. niger (n=10) was accounted for 6.81% of the total pool of strains isolated. Determinations were made for 13 antifungal drugs using the disk diffusion method. The A. niger exhibited high susceptibility to enilconazole, terbinafine, voriconazole, tioconazole and ketoconazole, low susceptibility to clotrimazole, miconazole and nystatin, and resistance to amphotericin B, itraconazole, pimaricin, fluconazole and 5-fluorocytosine. Minimum inhibitory concentration (MIC) was determined for 9 antifungal drugs using the micromethod of duplicate serial dilutions in a liquid medium. A. niger strains were most susceptible to enilconazole and voriconazole. MIC ranged from 0.0625 to 0.5 μg/ml for enilconazole, with MIC90-0.5 μg/ml and MIC50-0.125 μg/ml. The corresponding values for voriconazole were 0.25-1 μg/ml, 1 μg/ml and 0.5 μg/ml. MIC for amphotericin B and terbinafine ranged from 0.5 to 4 μg/ml, while the values for the remaining drugs were highly varied. MIC was measured by the gradient diffusion method using Etest® for 5 antifungal drugs: amphotericin B, fluconazole, itraconazole, ketoconazole and voriconazole. By far the highest susceptibility was obtained in the case of voriconazole, with MIC ranging from 0.0625 to 1 μg/ml. MIC for amphotericin B ranged from 0.25 to 4 μg/ml, for itraconazole and ketoconazole ranging from 0.5 to 16 μg/ml. Methods available for this purpose are not always applicable in field conditions. The present results indicate that the Etest® technique, due to its high percentage of agreement with the M 38-A microdilution method, should find application in medical and veterinary practice.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

      Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies