- Tytuł:
- Radicals initiated by gamma rays in selected amino acids and collagen
- Autorzy:
-
Przybytniak, Grażyna.
Sadło, Jarosław
Dąbrowska, Małgorzata
Zimek, Zbigniew - Powiązania:
- https://bibliotekanauki.pl/articles/146293.pdf
- Data publikacji:
- 2019
- Wydawca:
- Instytut Chemii i Techniki Jądrowej
- Tematy:
-
amino acid
collagen
EPR spectroscopy
gamma rays
aminokwas
kolagen
spektroskopia EPR
promienie gamma - Opis:
- Calf skin collagen and three amino acids essential for its structure, namely glycine, L-proline and 4-hydroxyl-L-proline, were irradiated with gamma rays up to a dose of 10 kGy. Conversion of radicals over time or after thermal annealing to selected temperatures was monitored by X-band electron paramagnetic resonance (EPR) spectroscopy. Some experimental spectra were compared with signals simulated based on literature data from the electron nuclear double resonance (ENDOR) studies. The following phenomena were confi rmed in the tested amino acids: abstraction of hydrogen atom (glycine, proline, hydroxyproline, collagen), deamination (glycine, hydroxyproline), decarboxylation (hydroxyproline). Chain scission at glycine residues, radiation-induced decomposition of side groups and oxidative degradation were observed in irradiated collagen. The decay of radicals in collagen saturated with water occurred at lower temperatures than in macromolecules having only structural water. The paramagnetic centres were the most stable in an oxygen-free atmosphere (vacuum). Radical processes deteriorated the structure of collagen; hence, radiation sterilization of skin grafts requires careful pros and cons analysis.
- Źródło:
-
Nukleonika; 2019, 64, 1; 11-17
0029-5922
1508-5791 - Pojawia się w:
- Nukleonika
- Dostawca treści:
- Biblioteka Nauki
Artykuł