- Tytuł:
- [5-(Benzyloxy)-1H-indol-1-yl]acetic acid, an aldose reductase inhibitor and PPARγ ligand
- Autorzy:
-
Soltesova Prnova, Marta
Majekova, Magdalena
Milackova, Ivana
Díez-Dacal, Beatriz
Pérez-Sala, Dolores
Ceyhan, Muserref
Banerjee, Sreeparna
Stefek, Milan - Powiązania:
- https://bibliotekanauki.pl/articles/1038997.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
aldose reductase inhibitor
PPARγ ligand
diabetes
indole - Opis:
- Based on overlapping structural requirements for both efficient aldose reductase inhibitors and PPAR ligands, [5-(benzyloxy)-1H-indol-1-yl]acetic acid (compound 1) was assessed for inhibition of aldose reductase and ability to interfere with PPARγ. Aldose reductase inhibition by 1 was characterized by IC50 in submicromolar and low micromolar range, for rat and human enzyme, respectively. Selectivity in relation to the closely related rat kidney aldehyde reductase was characterized by approx. factor 50. At organ level in isolated rat lenses, compound 1 significantly inhibited accumulation of sorbitol in a concentration-dependent manner. To identify crucial interactions within the enzyme binding site, molecular docking simulations were performed. Based on luciferase reporter assays, compound 1 was found to act as a ligand for PPARγ, yet with rather low activity. On balance, compound 1 is suggested as a promising lead-like scaffold for agents with the potential to interfere with multiple targets in diabetes.
- Źródło:
-
Acta Biochimica Polonica; 2015, 62, 3; 523-528
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł