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Wyszukujesz frazę "adsorption of protein" wg kryterium: Temat


Wyświetlanie 1-2 z 2
Tytuł:
How enzymes are adsorbed on soil solid phase and factors limiting its activity: a review
Autorzy:
Datta, Rahul
Anand, Swati
Moulick, Amitava
Baraniya, Divyashri
Imran Pathan, Shamina
Rejsek, Klement
Vranova, Valerie
Sharma, Meenakshi
Sharma, Daisy
Formanek, Pavel
Kelkar, Aditi
Powiązania:
https://bibliotekanauki.pl/articles/972694.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
adsorption of protein
adsorbed enzyme
enzymatic activity
soil solid phase
enzymes in soil
Opis:
A majority of biochemical reactions are often catalysed by different types of enzymes. Adsorption of the enzyme is an imperative phenomenon, which protects it from physical or chemical degradation resulting in enzyme reserve in soil. This article summarizes some of the key results from previous studies and provides information about how enzymes are adsorbed on the surface of the soil solid phase and how different factors affect enzymatic activity in soil. Many studies have been done separately on the soil enzymatic activity and adsorption of enzymes on solid surfaces. However, only a few studies discuss enzyme adsorption on soil perspective; hence, we attempted to facilitate the process of enzyme adsorption specifically on soil surfaces. This review is remarkably unmatched, as we have thoroughly reviewed the relevant publications related to protein adsorption and enzymatic activity. Also, the article focuses on two important aspects, adsorption of enzymes and factors limiting the activity of adsorbed enzyme, together in one paper. The first part of this review comprehensively lays emphasis on different interactions between enzymes and the soil solid phase and the kinetics of enzyme adsorption. In the second part, we encircle various factors affecting the enzymatic activity of the adsorbed enzyme in soil.
Źródło:
International Agrophysics; 2017, 31, 2
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of fibrinogen monolayers on mica by the colloid enhancement
Autorzy:
Nattich-Rak, M.
Adamczyk, Z.
Wasilewska, M.
Radziszewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/363140.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
adsorpcja lateksu
fibrynogen
koloidalne wzmocnienie warstw białkowych
monowarstwy fibrynogenu na mikach
adsorpcja nieodwracalna
cząstki koloidalne
adsorption of latex
fibrinogen
colloid enhancement of protein layers
fibrinogen monolayers by mica
irreversible adsorption
colloid particles
Opis:
Physicochemical properties of bovine plasma fibrinogen (Fb) in electrolyte solutions were characterised, comprising the diffusion coefficient (hydrodynamic radius), electrophoretic mobility (zeta potential) and the isoelectric point, found to be at pH=5.8. Similar electrokinetic measurements were performed for the mica substrate using the streaming potential cell. The kinetics of Fb adsorption on mica under diffusion-controlled transport was also studied. The surface concentration of Fb on mica was determined directly by AFM counting. By adjusting the time of adsorption, and bulk Fb concentration, monolayers of desired coverage were produced. It was confirmed that Fb adsorbed irreversibly on mica both at pH=3.5 and pH=7.4 (physiological value). It was postulated that in the latter case, where both the substrate and fibrinogen molecules were negatively charged, adsorption was due to heterogeneous charge distribution over the protein molecule. In order to check this hypothesis, monolayers of Fb on mica were studied using the colloid enhancement (CE) method, in which negatively and positively charged latex particles were used. Results of these experiments were quantitatively interpreted in terms of the fluctuation theory assuming that adsorption sites consisted of two and three Fb molecules, for pH=3.5 and 7.4, respectively. This allowed one to determine limits of applicability of the classical DLVO theory and confirm a heterogeneous charge distribution over the Fb molecule. It was also concluded that the CE method can be used for a sensitive determination of the Fb bulk concentration for the range inaccessible for other methods, i.e., for 0.1ppm and below. Another effect of vital significance confirmed in this work was that for some range of fibrinogen coverage both the negative and positive latexes efficiently adsorbed. This indicates the formation of superadsorbing surfaces having potential significance for various filtration processes.
Źródło:
Environmental Biotechnology; 2011, 7, 1; 1-11
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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