- Tytuł:
- Identification of actin from hepatoma Morris 5123 cells
- Autorzy:
-
Nowak, Dorota
Kochman, Agata
Malicka-Błaszkiewicz, Maria - Powiązania:
- https://bibliotekanauki.pl/articles/1044454.pdf
- Data publikacji:
- 1999
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
actin isoforms
affinity chromatography
hepatoma Morris 5123
actin - Opis:
- The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118; Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit α skeletal muscle actin (M^r 43000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti β actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.
- Źródło:
-
Acta Biochimica Polonica; 1999, 46, 4; 949-959
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł