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Wyszukujesz frazę "acanthamoeba" wg kryterium: Temat


Tytuł:
Amoebicidal or amoebostatic influence of disinfectants used in health facilities and laboratories on corneal strains of Acanthamoeba
Autorzy:
Baltaza, Wanda
Padzik, Marcin
Szaflik, Jacek P.
Dybicz, Monika
Hendiger, Edyta
Chomicz, Lidia
Powiązania:
https://bibliotekanauki.pl/articles/972267.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
in vitro dynamics
influence of disinfectants
Acanthamoeba strains
Opis:
Different Acanthamoeba species are amphizoic organisms distributed in wide range of habitats in natural and man-made environments; they are also detected on surfaces of equipment and accessories in health facilities. Some strains of the amoebae are causative agents of the vision-threatening human disease Acanthamoeba keratitis, mainly reported in contact lens wearers. An exceptional high resistance of Acanthamoeba trophozoites and particularly cysts to chemicals, disinfectants and drugs is believed as influencing difficulty resulting in unsuccessful therapeutic management. As Acanthamoeba keratitis is the serious medical problem worldwide, different chemicals with possible activity against environmental and clinical Acanthamoeba strains are tested. In our study, selected disinfectants used in health care settings and laboratories were tested and their efficacy against the corneal strains Acanthamoeba castellanii and A. polyphaga , and environmental A. castellanii Neff strain was assessed. Comparative assessment of results of the assays show that, apart from amoebistatic effects, the disinfectants indicated expected cysticidal efficacy.
Źródło:
Annals of Parasitology; 2017, 63, 3; 162-172
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The presence of pathogenic genotypes of free-living amoebae isolated from sandboxes in children’s playgrounds in the city of Poznan, Poland
Autorzy:
Cholewinski, M.
Solarczyk, P.
Derda, M.
Wojtkowiak-Giera, A.
Hadas, E.
Powiązania:
https://bibliotekanauki.pl/articles/5871.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pathogenic amoeba
genotype
free-living amoeba
amoeba
isolation
Acanthamoeba
human disease
granulomatous amoebic encephalitis
sandbox
child
playground
Poznan city
Polska
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The confocal microscopy, the tool for rapid in vivo diagnosis of Acanthamoeba keratitis - usefulness and limitations
Autorzy:
Chomicz, L.
Izdebska, J.
Padzik, M.
Baltaza, W.
Pionkowski, K.
Jasniewska, E.
Dybicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/5676.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
confocal microscopy
rapid diagnosis
in vivo diagnosis
Acanthamoeba keratitis
amoeba
parasite
usefulness
limitation
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
More Acanthamoeba Genotypes: Limits to Use rDNA Fragments to Describe New Genotype
Autorzy:
Corsaro, Daniele
Powiązania:
https://bibliotekanauki.pl/articles/763668.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Acanthamoeba, genotype, full gene sequence, partial gene sequence
Opis:
Strains of the genus Acanthamoeba are usually assigned to sequence types or genotypes according to pair-wise similarity values of the nuclear gene for the small subunit of ribosomal RNA. This classification system was established by comparing full or nearly full gene sequences, > 2000 bp. For practical reasons, diagnostic fragments of smaller lengths have been identified and used for rapid and economic identification of large number of strains. While the use of these small fragments in diagnostics applications remains valid when and only if the reference full sequence-type is available, we contest their use to identify and describe new genotypes. We report herein the case of a new genotype described on the basis of solely a small partial sequence and discuss the poor reliability of this fragment to correctly infer phylogenetic relationships, and its limits in the description of new genotypes of Acanthamoeba.
Źródło:
Acta Protozoologica; 2011, 50, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie obecnosci celulozy w scianie komorkowej cyst Acanthamoeba do celow diagnostycznych
Use of the presence of cellulose in cellular wall of Acanthamoeba cysts for diagnostic purposes
Autorzy:
Derba, M
Hadas, E.
Powiązania:
https://bibliotekanauki.pl/articles/839730.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
celuloza
choroby pasozytnicze
sciany komorkowe
cysty
Acanthamoeba
wykorzystanie
pierwotniaki
diagnostyka
pasozyty
budowa sciany komorkowej
Źródło:
Annals of Parasitology; 2009, 55, 1; 47-51
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie obecności celulozy w ścianie komórkowej cyst Acanthamoeba do celów diagnostycznych
Use of the presence of cellulose in cellular wall of Acanthamoeba cysts for diagnostic purposes
Autorzy:
Derba, M.
Hadasś, E.
Powiązania:
https://bibliotekanauki.pl/articles/2143498.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
celuloza
choroby pasozytnicze
sciany komorkowe
cysty
Acanthamoeba
wykorzystanie
pierwotniaki
diagnostyka
pasozyty
budowa sciany komorkowej
Opis:
Species identification within the genus Acanthamoeba is based predominantly on morphological and biochemical features. It is labor-intensive and requires cloning and axenization. We described a novel immunocytochemical method for the identification of Acanthamoeba spp. based on selective binding of Clostridium cellulovorans cellulase to protozoan cyst wall cellulose. Free-living amoebae isolated from different water sources by filtration and subsequent cultivation on non-nutrient agar were assigned to genera Acanthamoeba, Naegleria or Hartmannella using morphological taxonomic criteria. Tissues samples from experimentally infected mice were fixed in formalin and for sectioning embedded in paraffin or snap frozen. The Cellulose-Binding Domain of C. cellulovorans cellulase (CBD) obtained as a recombinant protein, were coupled to the fluorescent dye using Alexa Fluor®350, 488, 568 – Protein Labelling Kit or labelled with the biotin using EZ-Link Sulfo-NHS-Biotin. All coupling procedures were performed according to the methods provided by manufacturers. For staining with CBD conjugate, slides containing cysts collected from the agar plates or tissue sections were immersed with PBS and incubated with CBD for 30 min at room temperature, washed 3 times with PBS. For staining with CBD-biotin slides containing cysts were incubated with biotinylated CBD for 30 min at room temperature. Subsequent washings in changes of PBS were followed by the incubation with Strept ABComplex/HRP, for 30 min at room temperature, than 3,3 diaminobenzidine tetrahydrochloride was added for 15 min. Slides were rinsed with water, dried and examined in the light microscope. We showed that cellulose could be easily detected by immunofluorescence using conjugated CBD in the inner cyst wall of Acanthamoeba spp. The reference strains of Acanthamoeba spp. and all Acanthamoeba strains isolated from water and from tissues of infected animals gave positive reaction. CBD prepared as a biotynylated protein can be also used for the demonstration of Acanthamoeba cyst in infected tissues and environmental samples.
Źródło:
Wiadomości Parazytologiczne; 2009, 55, 1; 47-51
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pelzaki wolno zyjace jako nosiciele patogenicznych bakterii
Free-living amoebae as vehicles of pathogenic bacteria
Autorzy:
Derda, M
Sulek-Stankiewicz, A.
Hadas, E.
Powiązania:
https://bibliotekanauki.pl/articles/840392.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zapalenie rogowki
Balamuthia mandrillaris
Sappinia diploidea
zapalenie opon mozgowych i mozgu
choroby czlowieka
Acanthamoeba
bakterie patogenne
ameby
Naegleria
nosiciele
ziarniniakowe zapalenie mozgu
Opis:
The free-living amoebae are ubiquitous organisms. They are found in humid soil and all water reservoirs, i.e. fresh, sea, freezing and hot water. They mainly feed on bacteria. Pathogenic properties of amoebae and the mechanisms underlying pathological changes induced during human infection have not yet been fully elucidated. They are the causative agents of primary amoebic meningo-encephalitis (PAM), granulomatous amebic encephalitis (GAE), a chronic progressive disease of the central nervous system, amebic keratitis (AK), a chronic eye infection; amebic pneumitis (AP), a chronic lung infection, and skin infection. Only a few isolates are strongly and permanently pathogenic to humans. Some isolates lose their pathogenic properties after one passage. It has been assumed that such "temporary", unstable pathogenic properties of the amoebae may be caused by internal factors carried by them. It is generally known that the free-living amoebae may be naturally infected with pathogenic bacteria, which have the ability to survive for a long time and to proliferate in the amoebae cells. The role of the amoeba in the process of maintaining, propagating and transmitting human pathogens has not been well recognized. It has been suggested that some infections can be acquired by inhaling aerosols containing amoebae cells filled with bacteria. The presence of bacteria inside the free-living amoebae possess a great challenge to organisations responsible for testing and inspecting the quality and cleanliness of surface waters, swimming pools and drinking water intakes.
Źródło:
Annals of Parasitology; 2006, 52, 1; 1-7
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pełzaki wolno żyjące jako nosiciele patogenicznych bakterii
Free-living amoebae as vehicles of pathogenic bacteria
Autorzy:
Derda, M.
Sułek-Stankiewicz, A.
Hadaś, E.
Powiązania:
https://bibliotekanauki.pl/articles/2144083.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zapalenie rogowki
Balamuthia mandrillaris
Sappinia diploidea
zapalenie opon mozgowych i mozgu
choroby czlowieka
Acanthamoeba
bakterie patogenne
ameby
Naegleria
nosiciele
ziarniniakowe zapalenie mozgu
Opis:
The free-living amoebae are ubiquitous organisms. They are found in humid soil and all water reservoirs, i.e. fresh, sea, freezing and hot water. They mainly feed on bacteria. Pathogenic properties of amoebae and the mechanisms underlying pathological changes induced during human infection have not yet been fully elucidated. They are the causative agents of primary amoebic meningo-encephalitis (PAM), granulomatous amebic encephalitis (GAE), a chronic progressive disease of the central nervous system, amebic keratitis (AK), a chronic eye infection; amebic pneumitis (AP), a chronic lung infection, and skin infection. Only a few isolates are strongly and permanently pathogenic to humans. Some isolates lose their pathogenic properties after one passage. It has been assumed that such "temporary", unstable pathogenic properties of the amoebae may be caused by internal factors carried by them. It is generally known that the free-living amoebae may be naturally infected with pathogenic bacteria, which have the ability to survive for a long time and to proliferate in the amoebae cells. The role of the amoeba in the process of maintaining, propagating and transmitting human pathogens has not been well recognized. It has been suggested that some infections can be acquired by inhaling aerosols containing amoebae cells filled with bacteria. The presence of bacteria inside the free-living amoebae possess a great challenge to organisations responsible for testing and inspecting the quality and cleanliness of surface waters, swimming pools and drinking water intakes.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 1; 1-7
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pelzakobojcze ekstrakty roslinne
Autorzy:
Derda, M.
Hadas, E.
Thiem, B.
Sulek, A.
Powiązania:
https://bibliotekanauki.pl/articles/838231.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
olownik latkowaty
Solidago virgaurea
Acanthamoeba
malina moroszka
nawloc pospolita
Rubus chamaemorus
Pueraria lobata
ekstrakty roslinne
pasozyty czlowieka
zwalczanie pasozytow
nawloc waskolistna
czynniki chorobotworcze
parazytologia lekarska
aktywnosc pasozytobojcza
ameby
Solidago graminifolia
ziarniniakowe zapalenie mozgu
Źródło:
Annals of Parasitology; 2004, 50, 4; 715-721
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pełzakobójcze ekstrakty roślinne
Amebicidal plants extracts.
Autorzy:
Derda, M.
Hadaś, E.
Thiem, B.
Sułek, A.
Powiązania:
https://bibliotekanauki.pl/articles/2147585.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
olownik latkowaty
Solidago virgaurea
Acanthamoeba
malina moroszka
nawloc pospolita
Rubus chamaemorus
Pueraria lobata
ekstrakty roslinne
pasozyty czlowieka
zwalczanie pasozytow
nawloc waskolistna
czynniki chorobotworcze
parazytologia lekarska
aktywnosc pasozytobojcza
ameby
Solidago graminifolia
ziarniniakowe zapalenie mozgu
Opis:
The free-living amoebae from genus Acanthamoeba are the causative agents of granulomatous amebic encephalitis (GAE), a chronic progressive disease of the central nervous system; amebic keratitis (AK), a chronic eye infection; amebic pneumitis (AP), a chronic lung infection, and skin infection. Chemotherapy of Acanthamoeba infection is problematic. The majority of infections have been fatal. Only a few cases are reported to have been treated successfully with very highly toxic drugs. The therapy might be succeed, if the diagnosis and therapy is made at very early stage of infection. In our experiments we used the following plant extracts: Solidago virgaurea, Solidago graminifolia, Rubus chamaemorus, Pueraria lobata, and natural plants products as ellagic acid and puerarin. Those therapeutic agents and plants extracts have been tested in vitro for amebicidal or amebostatic activity against pathogenic Acanthamoeba spp. Our results showed that methanol extracts obtained from plants are active against axenic pathogenic Acanthamoeba sp. trophozoites in vitro at concentration below 0.1 mg/ml. Further studies are needed to investigate whether these extracts are also effective in vivo in animal model of infection with Acanthamoeba sp.
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 4; 715-721
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykrywanie obecności Acanthamoeba sp. w próbach środowiskowych i materiale pochodzącym od pacjentów przy pomocy techniki PCR
Autorzy:
Derda, M.
Hadaś, E.
Sułek, A.
Powiązania:
https://bibliotekanauki.pl/articles/2147593.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
proby srodowiskowe
material kliniczny
parazytologia lekarska
Acanthamoeba
lancuchowa reakcja polimerazy
metody wykrywania
ameby
pasozyty czlowieka
Źródło:
Wiadomości Parazytologiczne; 2004, 50, 4; 747
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykrywanie obecnosci Acanthamoeba sp.w probach srodowiskowych i materiale pochodzacym od pacjentow przy pomocy techniki PCR
Autorzy:
Derda, M
Hadas, E.
Sulek, A.
Powiązania:
https://bibliotekanauki.pl/articles/839696.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
proby srodowiskowe
material kliniczny
parazytologia lekarska
Acanthamoeba
lancuchowa reakcja polimerazy
metody wykrywania
ameby
pasozyty czlowieka
Źródło:
Annals of Parasitology; 2004, 50, 4; 747
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Basic energetic parameters of Acanthamoeba castellanii mitochondria and their resistance to oxidative stress
Autorzy:
Jarmuszkiewicz, Wieslawa
Antos-Krzeminska, Nina
Drachal-Chrul, Danuta
Matkovic, Karolina
Nobik, Wioletta
Pieńkowska, Joanna
Swida, Aleksandra
Woyda-Ploszczyca, Andrzej
Budzinska, Malgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1040753.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Acanthamoeba castellanii
mitochondria
hydrogen peroxide
oxidative stress
Opis:
The purpose of this study was establishing the basic energetic parameters of amoeba Acanthamoeba castellanii mitochondria respiring with malate and their response to oxidative stress caused by hydrogen peroxide in the presence of Fe2+ ions. It appeared that, contrary to a previous report (Trocha LK, Stobienia O (2007) Acta Biochim Polon 54: 797), H2O2-treated mitochondria of A. castellanii did not display any substantial impairment. No marked changes in cytochrome pathway activity were found, as in the presence of an inhibitor of alternative oxidase no effects were observed on the rates of uncoupled and phosphorylating respiration and on coupling parameters. Only in the absence of the alternative oxidase inhibitor, non-phosphorylating respiration progressively decreased with increasing concentration of H2O2, while the coupling parameters (respiratory control ratio and ADP/O ratio) slightly improved, which may indicate some inactivation of the alternative oxidase. Moreover, our results show no change in membrane potential, Ca2+ uptake and accumulation ability, mitochondrial outer membrane integrity and cytochrome c release for 0.5-25 mM H2O2-treated versus control (H2O2-untreated) mitochondria. These results indicate that short (5 min) incubation of A. castellanii mitochondria with H2O2 in the presence of Fe2+ does not damage their basic energetics.
Źródło:
Acta Biochimica Polonica; 2008, 55, 2; 349-356
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of growth at low temperature on the alternative pathway respiration in Acanthamoeba castellanii mitochondria.
Autorzy:
Jarmuszkiewicz, Wiesława
Frączyk, Olaf
Hryniewiecka, Lilla
Powiązania:
https://bibliotekanauki.pl/articles/1044102.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Acanthamoeba castellanii
low temperature adaptation
alternative oxidase
mitochondria
Opis:
Mitochondria of amoeba Acanthamoeba castellanii in addition to the conventional cytochrome pathway possess, like plant mitochondria, a cyanide-resistant alternative quinol oxidase. In mitochondria isolated from amoeba batch culture grown temporarily at low temperature (6°C), higher respiration was accompanied by lower coupling parameters as compared to control culture (grown at 28°C). In the presence of benzohydroxamate, respiratory rates and coupling parameters were similar in both types of mitochondria indicating that growth in cold conditions did not disturb the cytochrome pathway. Increased contribution of alternative oxidase in total mitochondrial respiration in low-temperature-grown amoeba cells was confirmed by calculation of its contribution using ADP/O measurements. Furthermore, in mitochondria from low-temperature- grown cells the content of the alternative oxidase was increased and correlated with the increase in the unstimulated and GMP-stimulated cyanide-resistant respiratory activity. A possible physiological role of higher activity of alternative oxidase as response to growth at a low temperature in unicellular organisms, such as amoeba, is discussed.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 729-737
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria.
Autorzy:
Jarmuszkiewicz, Wiesława
Czarna, Małgorzata
Sluse-Goffart, Claudine
Sluse, Francis
Powiązania:
https://bibliotekanauki.pl/articles/1043287.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Acanthamoeba castellanii
uncoupling protein
energy-dissipating
mitochondria
Opis:
Mitochondria of the amoeba Acanthamoeba castellanii possess a free fatty acid-activated uncoupling protein (AcUCP) that mediates proton re-uptake driven by the mitochondrial proton electrochemical gradient. We show that AcUCP activity diverts energy from ATP synthesis during state 3 mitochondrial respiration in a fatty acid-dependent way. The efficiency of AcUCP in mitochondrial uncoupling increases when the state 3 respiratory rate decreases as the AcUCP contribution is constant at a given linoleic acid concentration while the ATP synthase contribution decreases with respiratory rate. Respiration sustained by this energy-dissipating process remains constant at a given linoleic acid concentration until more than 60% inhibition of state 3 respiration by n-butyl malonate is achieved. The present study supports the validity of the ADP/O method to determine the actual contributions of AcUCP (activated with various linoleic acid concentrations) and ATP synthase in state 3 respiration of A. castellanii mitochondria fully depleted of free fatty acid-activated and describes how the two contributions vary when the rate of succinate dehydrogenase is decreased by succinate uptake limitation.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 533-538
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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