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Tytuł:
Effect of aqueous extract from Scutellaria baicalensis Georgi roots on CD4+ and CD8+ T cell responses during experimental infection with Trichinella spiralis in mice
Autorzy:
Piekarska, J.
Szczypka, M.
Gorczykowski, M.
Króliczewska, B.
Miśta, D.
Oszmiański, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087259.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Scutellaria baicalensis
Trichinella spiralis
immunomodulation
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 4; 501-510
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
1D-immunoblot as a species-specific diagnostic tool in human trichinellosis: yes or no?
Autorzy:
Bien, J.
Gozdzik, K.
Moskwa, B.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/6700.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
immunoblotting
diagnostic method
Trichinella spiralis
etiological agent
human infection
human trichinellosis
trichinellosis
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anti-Trichinella antibodies in the meat juice of different species of carnivores
Autorzy:
Cybulska, A.
Bien, J.
Moskwa, B.
Powiązania:
https://bibliotekanauki.pl/articles/5854.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
anti-Trichinella antibody
antibody
nematode
Trichinella
Trichinella spiralis
Trichinella britovi
Trichinella nativa
Trichinella pseudospiralis
meat juice
carnivore
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative ultrastructural studies of the alterations to mouse hepatic parenchyma during Trichinella spiralis or Toxocara canis infection
Autorzy:
Dabrowska, J.
Dybicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/6278.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
ultrastructure
alteration
mouse
mice
hepatic parenchyma
parenchyma
Trichinella spiralis
Toxocara canis
infection
pathological change
liver
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of CD4 and CD8 T cells in the small intestine of mice after probiotic treatment and Trichinella spiralis infection
Autorzy:
Dvoroznakova, E.
Buckova, B.
Hurnikova, Z.
Revajova, V.
Laukova, A.
Powiązania:
https://bibliotekanauki.pl/articles/5886.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
distribution
CD4 T cell
CD8 T cell
small intestine
mouse
mice
probiotic
treatment
Trichinella spiralis
animal infection
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The occurrence of Trichinella spiralis in raccoon (Procyon lotor)
Autorzy:
Cybulska, A.
Skopek, R.
Kornacka, A.
Popiolek, M.
Pirog, A.
Moskwa, B.
Powiązania:
https://bibliotekanauki.pl/articles/6729.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
animal disease
parasite
trichinellosis
parasitic nematode
occurrence
Trichinella spiralis
racoon
Procyon lotor
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badanie przydatnosci testu ELISA do wczesnego diagnozowania inwazji z rodzaju Trichinella u swin
The usefulness of ELISA test early serological detection of Trichinella spp. infection in pigs
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/838974.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka wczesna
pasozyty zwierzat
trzoda chlewna
Trichinella spiralis
parazytologia
inwazja pasozytnicza
przydatnosc
test ELISA
Źródło:
Annals of Parasitology; 2007, 53, 2; 149-151
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badanie przydatności testu ELISA do wczesnego diagnozowania inwazji z rodzaju Trichinella u świń
The usefulness of ELISA test early serological detection of Trichinella spp. infection in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2143857.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka wczesna
pasozyty zwierzat
trzoda chlewna
Trichinella spiralis
parazytologia
inwazja pasozytnicza
przydatnosc
test ELISA
Opis:
Trichinellosis is a parasitic zoonosis transmitted to humans through consumption of raw or undercooked meat from animals infected with nematodes of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease. The first step of the study was the optimization of a new ELISA method enabling an early and specific serological diagnosis of trichinellosis in pigs and wild boars using excretory−secretory (ES) antigens obtained from in vitro cultures of L1 T. spiralis. Serum samples were assayed for anti−T. spiralis IgG antibodies using the new ELISA protocol and a reference test — Standard manufactured by Institut Pourquier. The optimization involved the selection of suitable plates for antigen coating, dilution of sera and antibodies and their time of incubation. On the basis of the optimization a new ELISA procedure for the detection of IgG and IgM against T. spiralis was elaborated. Conventional, Iberian pigs and SPF (Specific Pathogen Free) pigs were infected with 200, 1000 and 20,000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies against excretory−secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project Trichiporse: „Safe pork and horse meat on EU markets: early and unbiased diagnostic tests for Trichinella”. Field samples of conventional pigs (1474) and wild boars (1784) were obtained from slaughter houses in different parts of Poland. Pigs were examined for the presence of Trichinella spp. using the artificial digestion method. Only four pigs were naturally infected with T. spiralis, the remaining were Trichinella larvae free. ELISA was used to examine IgG levels against L1 T. spiralis in pig and wild boar sera. The usefulness of ELISA for anti−IgG detection in pigs is usually limited by the nature of the antigen. The antigens were prepared in different laboratories: in Germany — Ag ES L1 T. spiralis (N), Italy — Ag ES L1 T. spiralis (W)) and in Poland — Ag ES L1 T. spiralis. Cut−off values for ELISA along with the estimated sensitivity and specificity were calculated using different methods: S/P%, M+3SD and ROC (Receiver Operating Characteristic). In SPF and Iberian pigs inoculated with 200, 1000 and 20,000 L1 T. spiralis, specific antibodies were detected 40, 30 and 25 dpi, respectively, with the use of the Standard (reference test). The analysis of the two ELISA procedures demonstrated a high sensitivity and specificity for the newly elaborated test utilizing the Ag ES L1 T. spiralis. In conventional pigs infected with 20,000 L1 T. spiralis specific antibodies were detected from 20 dpi when employing the new protocol. Similar results for the Standard and new ELISA test were obtained for serum samples of conventional pigs infected with 200 and 1000 larvae, which became positive from 40 dpi and 30 dpi, respectively. The results showed that both: the Standard and new protocols were comparable, and based on this, the new test was applied for further research. Results obtained adopting the new protocol with three antigens showed that two of them: Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis are similar. The specific IgG antibodies for infective doses of 200 and 1000 larvae for these antigens were detectable 40 and 30 dpi respectively. In pigs infected with the highest dose of T. spiralis larvae IgG anti bodies were detectable from 20 dpi when Ag ES L1 T. spiralis was used. These results strongly indicate that in examined pigs, the specific IgG response to T. spiralis infection is dose dependant. Of 1474 examined pig sera only 0.99% gave a positive signal against ES L1 T. spiralis antigen. Of 1784 examined wild boars sera only 0.68 % gave positive results using the new ELISA protocol. ELISA is a useful method for detecting specific IgG antibodies in pigs experimentally infected with different doses of T. spiralis and naturally infected pigs. In pigs the specific IgG response is dose dependant. The Ag ES L1 T. spiralis increases the specifity of the method and reduces false positive results. Simultaneous use of both methods: digestion and ELISA for the diagnosis of Trichinella in naturally infected pigs and wild boars may increase the chances of eliminating meat infected with T. spiralis larvae.
Źródło:
Wiadomości Parazytologiczne; 2007, 53, 2; 149-151
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Autorzy:
Moskwa, B.
Bień, J.
Cabaj, W.
Korinkova, K.
Koudela, B.
Kacprzak, E.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144348.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
human disease
serodiagnosis
diagnosis
trichinellosis
ELISA test
zoonotic disease
ELISA procedure
excretory-secretory antigen
Trichinella spiralis
human outbreak
Opis:
Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 231-238
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Autorzy:
Moskwa, B
Bien, J.
Cabaj, W.
Korinkova, K.
Koudela, B.
Kacprzak, E.
Stefaniak, J.
Powiązania:
https://bibliotekanauki.pl/articles/839966.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
human disease
serodiagnosis
diagnosis
trichinellosis
ELISA test
zoonotic disease
ELISA procedure
excretory-secretory antigen
Trichinella spiralis
human outbreak
Opis:
Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
Źródło:
Annals of Parasitology; 2006, 52, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144343.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw procedury otrzymywania antygenow ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarazonych swin
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840241.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Annals of Parasitology; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ wysokich ciśnień (UHP) na niektóre właściwości i stan higieniczny mięsa wieprzowego
Effect of high pressure (UHP) on some properties and hygienic state of pork meat
Autorzy:
Kłoczko, I.
Powiązania:
https://bibliotekanauki.pl/articles/228197.pdf
Data publikacji:
2006
Wydawca:
Wyższa Szkoła Menedżerska w Warszawie
Tematy:
ciśnienie izostatyczne
metoda UHP
dekontaminacja wysokim ciśnieniem
miano coli
Trichinella spiralis
Opis:
Próbki mięsa wieprzowego, pakowano próżniowo w torebki z folii PAPE, poddawano działaniu ciśnienia izostatycznego 400 Mpa i przechowywano w 4C do wystąpienia wyraźnych objawów zepsucia. W określonych odstępach czasu część próbek poddawano badaniom sensorycznym i mikrobiologicznym oraz instrumentalnym badaniom tekstury i barwy. Próbki prasowane dłużej zachowywały przydatność do spożycia i posiadały lepszą teksturę, niż próby kontrolne. Ujemną cechąpróbek ciśnieniowanych było nadmierne rozjaśnienie naturalnej czerwonej barwy mięsa. Ciśnieniowanie obniżało znacznie ogólną liczbę drobnoustrojów w mięsie, jednak w czasie przechowywania przyrost jednostek tworzących kolonie był w próbach prasowanych wyższy, niż w próbachkontrolnych. Wysokie ciśnienie niszczyło nieodwracalnie bakterie Escherichia coli. W oddzielnej serii doświadczeń z zastosowaniem ciśnień rzędu 50-300 MPa ustalono, że ciśnienie hydrostatyczne powyżej 200 MPa powoduje pełną dekontaminację mięsa zainfekowanego larwami włośnią Trichinella spiralis.
Samples of fresh pork meat were vacuum-packed in polyamide-polyethylene foil, subjected to isostatic pressure of 400 MPa and stored at 4°C until visible symptoms of spoiling. In definite time intervals certain numbers of the samples, as well as of non-pressurized control samples, were subjected to sensoric and instrumental quality assessments involving determinations of general appearence, flavour, hardness, colour, texture etc. both in raw and boiled state. Total no. of colony-forming units and coli titre were also determined. The pressurisation increased the shelf life of the meat and improved its sensoric properties except of the coulour which appeared too bright because of hemoglobine destruction. The pressure applied reduced the total no. of colony-forming units by about two log units, but the overall growth of bacteria during the storage was much more rapid in pressurised samples than that in the controls. The pressurisation destroyed completely and irreversibly the Escherichia coli in the meat samples.
Źródło:
Postępy Techniki Przetwórstwa Spożywczego; 2006, T. 16, nr 1, 1; 25-29
0867-793X
2719-3691
Pojawia się w:
Postępy Techniki Przetwórstwa Spożywczego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zwierzęta wolno żyjace i domowe jako stale utrzymujacy się rezerwuar włośnicy w Polsce
Wild and domestic animals as permanent Trichinella reservoir in Poland
Autorzy:
Cabaj, W.
Powiązania:
https://bibliotekanauki.pl/articles/2144339.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
Polska
pasozyty
nicienie
Warszawa konferencja
zwierzeta dziko zyjace
zywiciele
Trichinella britovi
wystepowanie
wlosien krety
choroby czlowieka
zwierzeta domowe
parazytologia
Trichinella spiralis
konferencje
Opis:
Backround. Since Owen first described Trichinella as a human patogen in 1835, the number of organisms comprising this genus has grown dramatically. This etiological agent of human trichinellosis shows worldwide distribution in domestic and/or sylvatic animals. Material and method. The aim of the presented paper was to determine the distribution of Trichinella species in wild animals such as red foxes, wolves, wild boars, and domestic pigs in Poland. Muscle samples from diaphragm and forelegs were collected from animals killed by hunters. Muscle larvae were recovered from the muscle after artificial digestion and identified at the species level by RAPD, PCR-RPLF and multiplex PCR. Results. Of 75 nematode isolates from red foxes: (Vulpes vulpes), 50 resulted as T. britovi, 6 T. spiralis, 6 were mixed infections of these two species. Fifteen Trichinella isolates remained unidentified. Of 97 nematode isolates from wild boars (Sus scrofa), 21 resulted as T. britovi, 69 T. spiralis, 2 were mixed infections of these two species. Five Trichinella isolates remained unidentified. Of 6 examined wolves (Canis lupus) killed in the Bieszczady region, 3 animals were positive against T. britovi. Of 6 examined raccon dogs (Nyctereutes procyonoides) from Pomorskie region, 2 animals were positive against T. spiralis. Of 21 nematode isolates from domestic pigs, 1 resulted as T. britovi and 21 as T. spiralis. Up to date, two Trichinella species are detected as the etiological agents of epidemiology among domestic and wildlife animal in Poland: T. britovi is the dominant species in red foxes and T. spiralis is the dominant species in wild boars and domestic pigs.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 175-179
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zwierzeta wolno zyjace i domowe jako stale utrzymujacy sie rezerwuar wlosnicy w Polsce
Wild and domestic animals as permanent Trichinella reservoir in Poland
Autorzy:
Cabaj, W
Powiązania:
https://bibliotekanauki.pl/articles/836583.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
Polska
pasozyty
nicienie
Warszawa konferencja
zwierzeta dziko zyjace
zywiciele
Trichinella britovi
wystepowanie
wlosien krety
choroby czlowieka
zwierzeta domowe
parazytologia
Trichinella spiralis
konferencje
Opis:
Backround. Since Owen first described Trichinella as a human patogen in 1835, the number of organisms comprising this genus has grown dramatically. This etiological agent of human trichinellosis shows worldwide distribution in domestic and/or sylvatic animals. Material and method. The aim of the presented paper was to determine the distribution of Trichinella species in wild animals such as red foxes, wolves, wild boars, and domestic pigs in Poland. Muscle samples from diaphragm and forelegs were collected from animals killed by hunters. Muscle larvae were recovered from the muscle after artificial digestion and identified at the species level by RAPD, PCR-RPLF and multiplex PCR. Results. Of 75 nematode isolates from red foxes: (Vulpes vulpes), 50 resulted as T. britovi, 6 T. spiralis, 6 were mixed infections of these two species. Fifteen Trichinella isolates remained unidentified. Of 97 nematode isolates from wild boars (Sus scrofa), 21 resulted as T. britovi, 69 T. spiralis, 2 were mixed infections of these two species. Five Trichinella isolates remained unidentified. Of 6 examined wolves (Canis lupus) killed in the Bieszczady region, 3 animals were positive against T. britovi. Of 6 examined raccon dogs (Nyctereutes procyonoides) from Pomorskie region, 2 animals were positive against T. spiralis. Of 21 nematode isolates from domestic pigs, 1 resulted as T. britovi and 21 as T. spiralis. Up to date, two Trichinella species are detected as the etiological agents of epidemiology among domestic and wildlife animal in Poland: T. britovi is the dominant species in red foxes and T. spiralis is the dominant species in wild boars and domestic pigs.
Źródło:
Annals of Parasitology; 2006, 52, 3; 175-179
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 108

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