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    Wyświetlanie 1-48 z 48
    Tytuł:
    Methods of eliminating contaminations in tissue cultures on the example of selected ornamental plant species
    Autorzy:
    Parzymies, M.
    Dabski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/951204.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    tissue culture
    contamination
    ornamental plant
    Clematis
    biological contamination
    micropropagation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of additional additives on the axillary shoot micropropagation of medicinal plant Aegle marmelos (L.) Corrêa
    Autorzy:
    Mandal, Shubhjeet
    Parsai, Abhishek
    Tiwari, Piyush Kumar
    Nataraj, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1031277.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Additives
    Aegle marmelos
    Amino Acids
    Axillary Shoot Culture
    Micropopgation
    PGR
    Tissue Culture
    Opis:
    India is an immense medicinal plant repository, used in traditional medical treatments. The use of herbal medicine is growing day by day due to toxicity and allopathic drug effects. About 90 percent of the phytochemicals marketed come from wild resources and over 120 major phytochemistry come from plants. Aegle marmelos leaves contained π-sitosterol, aegelin, lupeol, rutin, marmesinine, flavone, glycoside, Oisopentenyl halfordiol, marmeline, and phenylethyl cinnamides. The additional growth promoters show its effect on tissue culture of plant.
    Źródło:
    World News of Natural Sciences; 2021, 34; 54-71
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Economics analysis of Seed Yam production in Aeroponic system
    Autorzy:
    Acheampong, Patricia Pinamang
    Quain, Marian Dorcas
    Appiah-Kubi, David
    Osei-Adu, Jonas
    Ennin, Stella Ama
    Osei, Kingsley
    Powiązania:
    https://bibliotekanauki.pl/articles/1892087.pdf
    Data publikacji:
    2020-07-08
    Wydawca:
    Uniwersytet Przyrodniczy w Poznaniu. Wydawnictwo Uczelniane
    Tematy:
    Benefit-cost ratio
    net revenue
    payback period
    private sector
    tissue culture
    Opis:
    The supply of seed yams for intensive yam production is hindered by many constraints, including diseases and pest infestations as well as the unavailability of quality planting material. The combination of tissue culture and aeroponics system is perceived to be the way towards clean and adequate supply of seed yam for enhanced yam production. However, the system is considered as expensive for any individual to implement. In order to encourage private sector to participate in this venture, the economic analysis determining the feasibility and viability of using aeroponics in seed yam production was performed. Using data from established tissue culture and aeroponics system in Ghana, the fixed cost and variable cost parameters as well as production costs were obtained. Results revealed that total cost of building aeroponics structure amounted to GH₵ 94,178.00 (USD 17,938.70). Annual cost of aeroponics structure was GH₵ 9,417.82 (USD 1,793.87). Annual total cost of production totalled GH₵ 204,391.75 (USD 38,931.61). Annual net revenue was GH₵ 75,888.00 (USD 14,454.86). Payback period was 15 months and benefit cost ratio was 1.4. Aeroponics system for seed yam production is therefore profitable since short period would be needed to recoup investment. For food security and creation of workplaces, government could partner with the private sector in the establishment of aeroponics systems to increase yam production and export.
    Źródło:
    Journal of Agribusiness and Rural Development; 2020, 56, 2; 125-132
    1899-5241
    Pojawia się w:
    Journal of Agribusiness and Rural Development
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nuclear morphology, polyploidy, and chromatin elimination in tissue culture of Allium fistulosum L.
    Autorzy:
    Joachimiak, A
    Ilnicki, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/59216.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    chromatin
    heterochromatin
    tissue culture
    polyploidy
    Alliaceae
    morphology
    Monocotyledoneae
    micronucleus
    Allium fistulosum
    Opis:
    The morphology of cell nuclei in callus obtained from root-tip meristems of Allium fistulosum L. (Monocotyledoneae, Alliaceae) was analysed. The most interesting phenomena observed in long-term callus culture were the different mechanisms of cell polyploidization, enlargement of telomeric segments of heterochromatin, and extensive chromatin elimination, associated with instability of nuclei size and DNA content. Protruding heterochromatin "spikes" were observed on the surface of some di- and polyploid nuclei. The presence of these spikes was connected with the formation of small heterochromatic micronuclei frequently found in the cytoplasm. It is suggested that these micronuclei are produced by direct elimination of heterochromatin from the interphase nuclei. Polyploid cells accumulated with each successive cell collection. The ploidy level attained by highly polyploid cells was 15C-220C. The shape of the nuclei and heterochromatin distribution suggest that polyploid nuclei in A. fistulosum tissue culture are produced by endoreduplication and by restitution cycles.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of light conditions on the morphogenetic and biochemical response of selected ornamental plant species under in vitro conditions: a mini-review
    Autorzy:
    Kulus, D.
    Woźny, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096967.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    horticulture
    LED panels
    plant tissue culture
    regeneration
    secondary metabolites
    somatic
    embryogenesis
    Opis:
    In vitro tissue culture technique, especially micropropagation, is attracting increasing attention in the production of ornamental plants. This technique will probably dominate the horticultural market in the near future. Light is one of the several factors affecting the success of in vitro plant tissue cultures. It directly affects the widely understood morphogenetic response of the explant, i.e., the ability of the explant to grow or regenerate, produce roots, etc. Lighting conditions provided during the in vitro stage may also greatly affect the plant vigor after transferring to nonsterile conditions. Moreover, the necessity of providing artificial light significantly contributes to the total cost of maintaining tissue cultures (related to energy consumption and the need to cool down the heat generated by lamps). Light quantity (intensity) and quality (spectral composition) are the two main parameters that determine its influence on in vitro cultures. This impact depends on the species and other accompanying factors. The aim of this mini-review is to summarize information on the influence of light on the morphogenetic and biochemical response of explants of some selected ornamental plant species grown under in vitro conditions.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 75-83
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro propagation of Nepalese orchids: a review
    Autorzy:
    da Silva, J.A.T.
    Acharya, K.P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1932.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    in vitro propagation
    Nepal
    Orchidaceae
    orchid
    seed germination
    tissue culture
    germplasm
    Źródło:
    Journal of Horticultural Research; 2014, 22, 2
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.
    Autorzy:
    Zhang, Yun-Wei
    Zhou, Yun-Zhuan
    Lu, Hai-Bo
    Zheng, Deng-Yu
    Huang, Yan-Hua
    Powiązania:
    https://bibliotekanauki.pl/articles/952058.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    saccharum spontaneum l.
    sps gene
    cloning
    expression
    tissue culture
    genetic transformation
    Opis:
    Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro responses of various explants of Fagus sylvatica
    Autorzy:
    Hazubska-Przybyl, T.
    Chmielarz, P.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41768.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    European beech
    plant response
    explant
    Fagus sylvatica
    micropropagation
    culture condition
    tissue culture
    in vitro culture
    Opis:
    There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
    Źródło:
    Dendrobiology; 2015, 73
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro cultures of animal and human cells
    Autorzy:
    Slomski, R.
    Nowak, A.
    Hryhorowicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/951215.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    in vitro culture
    tissue culture
    animal cell
    human cell
    homeostasis
    fluorescence in situ hybridization
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phenolics in undifferentiated tissue cultures of Cichorieae
    Autorzy:
    Malarz, J.
    Stojakowska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/79813.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    phenolics
    tissue culture
    Lactuca virosa
    Cichorium intybus
    Lactuca aculeata
    sesquiterpene lactone
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A mathematical model of HIV-1 infection including the saturation effect of healthy cell proliferation
    Autorzy:
    Kouche, M.
    Ainseba, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/907704.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Zielonogórski. Oficyna Wydawnicza
    Tematy:
    zakażenie HIV
    stabilność
    hodowla tkanek
    HIV
    periodic oscillations
    persistence
    stability
    tissue culture
    Opis:
    In this paper we derive a model describing the dynamics of HIV-1 infection in tissue culture where the infection spreads directly from infected cells to healthy cells trough cell-to-cell contact. We assume that the infection rate between healthy and infected cells is a saturating function of cell concentration. Our analysis shows that if the basic reproduction number does not exceed unity then infected cells are cleared and the disease dies out. Otherwise, the infection is persistent with the existence of an infected equilibrium. Numerical simulations indicate that, depending on the fraction of cells surviving the incubation period, the solutions approach either an infected steady state or a periodic orbit.
    Źródło:
    International Journal of Applied Mathematics and Computer Science; 2010, 20, 3; 601-612
    1641-876X
    2083-8492
    Pojawia się w:
    International Journal of Applied Mathematics and Computer Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro culture as a powerful method for conserving Iranian ornamental geophytes
    Autorzy:
    Hesami, M.
    Naderi, R.
    Yoosefzadeh-Najafabadi, M.
    Maleki, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80634.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    in vitro culture
    plant tissue culture
    ornamental plant
    geophyte
    cryopreservation
    genetic resource
    biodiversity
    germplasm
    Iran
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate
    Autorzy:
    Prausova, R.
    Kozelkova, Z.
    Safarova, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/57604.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    acclimatization
    in vitro culture
    Potamogeton praelongus
    plant tissue culture
    growth experiment
    plantlet size
    substrate type
    Opis:
    The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of stress conditions on acclimation of magnolia Magnolia x soulangiana Soul.-Bod.
    Wpływ warunków stresu na aklimatyzację magnolii Magnolia x soulangiana Soul.-Bod.
    Autorzy:
    Kamenicka, A.
    Lanakova, M.
    Cerna, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/808264.pdf
    Data publikacji:
    2002
    Wydawca:
    Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
    Tematy:
    stress condition
    acclimation
    magnolia
    Magnolia x soulangiana
    tissue culture
    leaf cell
    donor plant
    Opis:
    Under a scanning microscope, leaves from tissue cultures (after 8 weeks in vitro culturing), from 1 year old regenerants and from the 100 years old donor tree were studied. More abnormalities were observed in the tissue culture compared to regenerant and donor. During tissue culture transfer rapid, leaf dehydration occurs due to defective regulation mechanisms making stomata of tissue culture stay open. Epidermis as well as stomata cells were observed already on the leaves in in vitro conditions, but they become physiologically functioning only after transfer into conditions of natural environment.
    Przy zastosowaniu mikroskopu scaningowego porównywano cechy budowy komórek liści pochodzących z 100 letniej rośliny matecznej, 8 tygodniowych kultur tkankowych oraz 1 rocznych regeneratów. W regenerantach z kultur tkankowych obserwowano więcej nienormalności w budowie komórek epidermy, komórek szparkowych i komórek przyszparkowych w porównaniu z komórkami pochodzącymi z rośliny matecznej. Podczas przenoszenia tkanek w kulturze in vitro obserwowano odwodnienie tkanek liści a mimo to komórki szparkowe pozostawały otwarte. Komórki epidermy oraz komórki szparkowe były obecne na liściach hodowli in vitro jednak stawały się one fizjologicznie funkcjonalne tylko wtedy gdy zostały przeniesione do warunków naturalnych.
    Źródło:
    Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 481, 1
    0084-5477
    Pojawia się w:
    Zeszyty Problemowe Postępów Nauk Rolniczych
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rozmnazanie ziemniaka 'in vitro' w Instytucie Ziemniaka w Boninie
    Autorzy:
    Zaklukiewicz, K
    Sekrecka, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/835057.pdf
    Data publikacji:
    1992
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    ziemniaki
    kultury tkankowe
    rozmnazanie roslin
    hodowla in vitro
    potato
    tissue culture
    plant propagation
    in vitro culture
    Źródło:
    Ziemniak Polski; 1992, 3; 41-44
    1425-4263
    Pojawia się w:
    Ziemniak Polski
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Embryogenic callus induction and differentiation in silver fir (Abies alba Mill.) tissue cultures
    Autorzy:
    Nawrot-Chorabik, K
    Powiązania:
    https://bibliotekanauki.pl/articles/41643.pdf
    Data publikacji:
    2008
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    silver fir
    Abies alba
    tissue culture
    embryogenic suspensor mass
    somatic embryogenesis
    callus induction
    differentiation
    Opis:
    The research was conducted on explants of silver fir (Abies alba Mill.) deriving from several forest districts in southern Poland. The study encompassed the influence of the origin of plant material, type of explants, kind of substances used for explants sterilization, PPM and the type of medium on the ability to form embryogenic callus and to develop somatic embryos in silver fir explants. From the plant material collected in three sites, 57 clones were obtained from mature zygotic embryos; this produced an embryogenesis frequency of 6%. Embryogenic callus was obtained with a diameter of 65–70 mm depending on the material origin. The best medium for development of callus inducted on embryos isolated from mature silver fir seeds was the SH medium. Somatic embryos were formed in a globular stadium (24 pieces) on this medium. The 10% solution of NaOCl (used for 15 minutes) turned out to be the most effective substance for seed sterilization.
    Źródło:
    Dendrobiology; 2008, 59; 31-40
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants
    Autorzy:
    Li, J.
    Kang, Y.
    Qiang, S.
    Peng, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/56457.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    propagation
    goldenrod
    Solidago canadensis
    leaf
    nodal explant
    callus formation
    micropropagation
    adventitious shoot
    tissue culture
    Opis:
    Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog's medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation, rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2012, 81, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative analysis of cat bone marrow and adipose tissue cell cultures
    Autorzy:
    Mazurkevych, A.
    Malyuk, M.
    Kovpak, V.
    Kovpak, O.
    Kharkevych, Y.
    Jakubczak, A.
    Gryzinska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087658.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    stem cells
    bone marrow cell culture
    adipose tissue cell culture
    cytogenetic assays
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 549-557
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative study on tissue culture induced variation identified with metAFLP and RP – HPLC in barley and triticale regenerants
    Autorzy:
    Machczynska, J.
    Orlowska, R.
    Ogorek, K.A.
    Bednarek, P.T.
    Powiązania:
    https://bibliotekanauki.pl/articles/951298.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    tissue culture
    in vitro culture
    plant regeneration
    genetic change
    DNA methylation
    histone modification
    high performance liquid chromatography
    barley
    triticale
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Neural modeling of plant tissue cultures: a review
    Autorzy:
    Zielinska, S.
    Kepczynska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/81293.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    artificial neural network
    biomass
    plant tissue
    neural model
    tissue culture
    in vitro condition
    micropropagation
    radial neural network
    neural network
    somatic embryo
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A non caloric sweetener Stevia rebaudiana Bertoni - tissue cultured plantlets for organic farming and home gardening
    Autorzy:
    Tomaszewska-Sowa, M.
    Figas, A.
    Sawilska, A.K.
    Powiązania:
    https://bibliotekanauki.pl/articles/59692.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
    Tematy:
    non-caloric sweetener
    sweetener
    Stevia rebaudiana
    micropropagation
    plant growth regulator
    tissue culture
    plantlet
    organic farming
    home garden
    Źródło:
    Infrastruktura i Ekologia Terenów Wiejskich; 2015, IV/3
    1732-5587
    Pojawia się w:
    Infrastruktura i Ekologia Terenów Wiejskich
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adjustments to in vitro culture conditions and associated anomalies in plants
    Autorzy:
    Isah, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/19921.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    plant physiology
    plant tissue culture
    plant regeneration
    in vitro culture
    somaclonal variation
    hyperhydricity
    fasciation
    cresting zob.fasciation
    acclimatization
    plant
    anomaly
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
    Autorzy:
    Siong, P.K.
    Taha, R.M.
    Rahiman, F.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/19314.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    somatic embryogenesis
    plant regeneration
    hypocotyl
    leaf explant
    Brassica oleracea var.botrytis
    cauliflower
    embryogenic callus
    callus
    tissue culture
    Opis:
    We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Withania coagulans (Stocks) Dunal: biotechnological achievements and perspectives
    Autorzy:
    Teixera da Silva, J.A.
    Kher, M.M.
    Soner, D.
    Nataraj, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1928.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    Withania coagulans
    medicinal plant
    Solanaceae
    shoot regeneration
    rooting
    in vitro
    micropropagation
    tissue culture
    random amplified polymorphic DNA
    Źródło:
    Journal of Horticultural Research; 2015, 23, 1
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of in vitro culture techniques to barley [Hordeum vulgare L.] improvement
    Autorzy:
    Ullrich, S E
    Kleinhofs, A.
    Hou, L.
    Jones, B.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044453.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    tissue culture
    doubled haploid
    mutagenesis
    breeding programme
    anther culture
    in vitro
    callus culture
    somaclonal variation
    barley
    mutation
    Hordeum vulgare
    embryo
    molecular biology
    Opis:
    Several aspects of in vitro culture have potential for cereal improvement. This paper focuses on evaluation of somaclonal variation (SV) from immature embryo callus culture, and doubled haploid (DH) production via anther culture in barley. Genetically stable SV was observed for several seedling morphological traits such as albino, yellow, light green and lethal. SV occurred at approximately half the frequency of azide-induced mutagenesis. The potential for widespread application of anther culture-mediated DH production in barley breeding and genetic studies was increased through culture procedure improvements and understanding the inheritance of anther culture response. Methodology improvements included substitution of inexpensive gelrite for expensive ficoll or agarose, ability to grow anther donor plants under field as well as growth chamber conditions and flexibility in cold pretreatment/storage of anther donor spikes for 4-6 weeks prior to anther plating. From diallel analysis, inheritance of anther culture response was complex with additive and dominance effects for embryoid formation, total plant regeneration and green plant regeneration and reciprocal effects (maternal) for green plant regeneration. High x low responder crosses generated F₁’s that were intermediate in response and low x low crosses sometimes produced F₁ heterosis for green plant regeneration. Therefore, some recalcitrant types appear to be usable in anther culture DH production systems within a breeding program.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 49-58
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potential possibilities of the induction of European beech (Fagus sylvatica ) tissue cultures from various types of explants
    Autorzy:
    Hazubska-Przybyl, T.
    Chmielarz, P.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/951305.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Fagus sylvatica
    European beech
    tissue culture
    reproduction
    vegetative propagation
    somatic embryogenesis
    organogenesis
    2,4-dichlorophenoxyacetic acid
    benzyladenine
    zeatin
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ metoksychloru na hodowlę tkankową nerki małpy Cercopithecus aethiops i płuca zarodka ludzkiego
    Vlijanie metoksihlora nl kletki pocek martycszki Cercopithecus aethiops i kletki legkih celoveceskogo jambriona
    The influence of methoxychlor on the tissue culture of the kidney of the monkey Cercopithecus aethiops and of the lung of human embryo
    Autorzy:
    Przezdziecki, Z.
    Polna, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/874038.pdf
    Data publikacji:
    1970
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    Cercopithecus aethiops
    nerki
    malpy
    pluca
    zarodek ludzki
    hodowla tkankowa
    metoksychlor
    izomery
    kidney
    monkey
    liver
    embryo
    tissue culture
    metoxychlor
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 1970, 21, 1
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Art as a laboratory – Guy Ben-Ary’s work
    Autorzy:
    Szykowna, Sylwia
    Powiązania:
    https://bibliotekanauki.pl/articles/923265.pdf
    Data publikacji:
    2019-06-15
    Wydawca:
    Uniwersytet im. Adama Mickiewicza w Poznaniu
    Tematy:
    bio art
    posthumanism
    new media art
    laboratory
    tissue culture
    collectivity
    Ars Electronica
    art world
    Guy Ben-Ary
    SymbioticA
    Opis:
    The present paper deals with the work of an Israeli artist, Guy Ben-Ary. His work is a prime example of artistic practice in the field of bio art. Bio art provokes critical thinking about the place and role of people in today’s world. The main purpose of the article is to describe changes in contemporary artistic practices within the framework of art as a laboratory, the aim of which is to study reality.  
    Źródło:
    Images. The International Journal of European Film, Performing Arts and Audiovisual Communication; 2019, 25, 34
    1731-450X
    Pojawia się w:
    Images. The International Journal of European Film, Performing Arts and Audiovisual Communication
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Liquid Perfluorochemicals as Flexible and Efficient Gas Carriers Applied in Bioprocess Engineering: An Updated Overview and Future Prospects
    Autorzy:
    Pilarek, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/185200.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ciecz perfluorochemiczna
    fluorowęgiel
    gaz ciekły
    rozpuszczalność
    hybrydowy system hodowli
    liquid perfluorochemical
    fluorocarbon
    liquid gas carrier
    solubility
    cell/tissue culture
    hybrid culture system
    Opis:
    Fully synthetic, biochemically inert and water-immiscible liquid perfluorochemicals (PFCs) are recognised as flexible liquid carriers/scavengers of gaseous compounds (respiratory gases mainly, i.e. O2 and CO2) and increasingly applied in bioprocess engineering. A range of unmatched physicochemical properties of liquid PFCs, i.e. outstanding chemo- and thermostability, extremely low surface tension, simultaneous hydro- and lipophobicity, which result from carbon chain substitution with fluorine atoms (the most electronegative chemical element) and the presence of intramolecular C–F bonds (the strongest single bond known in organic chemistry) have been described in detail. Exceptional propensity to solubility of respiratory gases in liquid perfluorinated compounds has been widely discussed. Advantages and disadvantages of bioprocess applications of liquid PFCs in the form of a pure PFC as well as in an emulsified form have been pointed out. A liquid PFC-mediated mass transfer intensification in various types of microbial, plant cell and animal cell culture systems: from miniaturised microlitre-scale cultures, via biomaterial-based scaffolds containing culture systems, to litre-scale bioreactors, has been reviewed and elaborated on bearing in mind the benefits of bioprocesses.
    Źródło:
    Chemical and Process Engineering; 2014, 35, 4; 463-487
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of Allium aflatunense B. Fedtsch. ‘Purple Sensation’ and Allium karataviense Regel. ‘Ivory Queen’ regenerative capabilities in tissue culture
    Porównanie zdolnosci regeneracyjnych Allium aflatunense B. Fedtsch. 'Purple Sensation' i Allium karataviense Regel. 'Ivory Queen' w kulturze in vitro
    Autorzy:
    Kozak, D.
    Stelmaszczuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542509.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    comparison
    Allium aflatunense
    ornamental plant
    Allium karataviense
    regenerative capability
    tissue culture
    inflorescence stem
    leaf base
    polarity explant
    shoot multiplication
    Opis:
    Allium aflatunense and Allium karataviense are valuable bulbous plants grown in a garden. Moreover, Allium aflatunense is one of the most decorative species for cut flowers industry. These plants characterize with a low multiplication rate. Immature inflorescence stem sections and leaf bases isolated from ‘Allium aflatunense ‘Purple Sensation’ and Allium karataviense ‘Ivory Queen’ bulbs in October were used as source of explants for shoot regeneration. The use of a two – step sterilization procedure (2% Cl- – 30 min and 1% Cl- –15 min) gave much better results than a one – step (2% Cl- – 30 min). Inflorescence explants in inverted orientation were cultured on a Murashige and Skoog [1962] medium containing BA in concentration of 2 mg·dm⁻³ and NAA in concentration of 1 mg·dm⁻³. Leaf bases placed in normal and inverted orientation were incubated on MS medium supplemented with: BA 2 mg·dm⁻³ plus NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ plus NAA 1 mg·dm⁻³ and TDZ 1 mg·dm⁻³ plus NAA 1 mg·dm⁻³. In subsequent experiments clumps consisting of 2 shoots were cultured on MS medium containing BA 0.5, 1, 2 mg·dm⁻³ with NAA 0.1 mg·dm⁻³ and BA 2 mg·dm⁻³ with IAA 0.1 mg·dm⁻³ for 6 weeks. A multiplication rate during four subcultures on medium with addition of BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ was also examined. Explants from young inflorescences and from immature leaf bases were found effective to form shoots. The orientation of leaf explants did not have a significant effect on the number of regenerating shoots. 5.7 to 7.5 shoots were achieved for Allium aflatunense ‘Purple Sensation’ on studied media during 6 weeks of multiplication and 3.5 to 5.1 shoots for Allium karataviense ‘Ivory Queen’. Multiplication rate of 31.3 shoots for Allium aflatunense ‘Purple Sensation’ and 26.6 shoots for Allium karataviense ‘Ivory Queen’ was possible to obtain on medium supplemented with BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ after 32 weeks of culture.
    Allium aflatunense i Allium karataviense to cenne rośliny cebulowe do uprawy w ogrodach. Ponadto Allium aflatunense jest jednym z najbardziej dekoracyjnych gatunków uprawianym na kwiat cięty. Rośliny te charakteryzują się niskim współczynnikiem rozmnażania. Źródłem materiału do zapoczątkowania kultur były fragmenty młodych pędów kwiatostanowych i nasady liści izolowane w paĨdzierniku z Allium aflatunense ‘Purple Sensation’ i Allium karataviense ‘Ivory Queen’. Zastosowanie sterylizacji dwukrotnej (2% Cl- – 30 min i 1% Cl- –15 min) dało lepsze rezultaty niż sterylizacja pojedyncza. Eksplantaty z pędów kwiatostanowych wykładano w pozycji odwrotnej do naturalnej i kultywowano na pożywce Murashige i Skooga [1962] zawierającej BA 2 mg·dm⁻³ i NAA 1 mg·dm⁻³. Podstawy liści układano w pozycji naturalnej i odwróconej i inkubowano na pożywce MS z dodatkiem: BA 2 mg·dm⁻³ + NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ + NAA 1 mg·dm⁻³ i TDZ 1 mg·dm⁻³ + NAA 1 mg·dm⁻³. W kolejnym doświadczeniu zespoły pędów zawierające 2 pędy namnażano na pożywkach MS uzupełnionych: BA 0,5; 1; 2 mg·dm⁻³ oraz NAA 0,1 mg·dm⁻³ i BA 2 mg·dm⁻³ oraz IAA 0,1 mg·dm⁻³ podczas 6 tygodni. Oceniono również współczynnik namnażania pędów podczas 4 pasaży na pożywce z dodatkiem BA 0,5 mg·dm⁻³ i NAA 0,1 mg·dm⁻³. Fragmenty młodych pędów kwiatostanowych i podstawa liści wykazywały duże zdolności regeneracyjne pędów. Sposób ułożenia eksplantatów nie wywierał istotnego wpływu na liczbę regenerujących pędów. Na badanych pożywkach uzyskano od 5,7 do 7,5 pędów Allium aflatunense ‘Purple Sensation’ i od 3,5 do 5,1 pędów Allium karataviense ‘Ivory Queen’ podczas 6 tygodni namnażania. Podczas 32 tygodni namnażania pędów na pożywce zawierającej BA 0,5 mg·dm⁻³ i NAA 0,1 mg·dm⁻³ uzyskano 31,3 pędów Allium aflatunense ‘Purple Sensation’ i 26,6 pędów of Allium karataviense ‘Ivory Queen’.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2013, 12, 6; 197-213
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]
    Autorzy:
    Kraj, W
    Dolnicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/56550.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Plant Preservative Mixture
    plant physiology
    tissue culture
    tree bud
    Fagus sylvatica
    sterilization
    beech
    sterility
    in vitro
    embryo
    European beech
    bud
    Opis:
    Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)
    Kwitnienie i mikropropagacja in vitro lisianthusa (Eustoma grandiflorum) w reakcji na regulatory wzrostu roslin (NAA i BA)
    Autorzy:
    Kaviani, B.
    Zamiraee, F.
    Zanjani, S.B.
    Tarang, A.
    Torkashvand, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542950.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro culture
    ornamental plant
    flowering
    micropropagation
    Lisianthus
    Eustoma grandiflorum
    plant response
    plant growth regulator
    NAA potassium salt
    axillary bud
    callus
    plant tissue culture
    Opis:
    In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
    Kwitnienie i mikropropagacja in vitro są użyteczne w programach hodowli roślin oraz produkcji komercyjnej ważnych roślin ozdobnych. Warunki in vitro, łącznie ze składnikami pożywek, rodzajem, stężeniem oraz proporcją regulatorów wzrostu roślin, a także warunkami hodowli, w sposób istotny wpływają na kwitnienie i mikropropagację in vitro. Nie istnieje żadne badanie dotyczące kwitnienia in vitro lisanthiusa (Eustoma grandiflorum). W niniejszym badaniu opracowano kwitnienie i wysoką częstotliwość mikropropagacji in vitro dla E. grandiflorum, który jest rośliną ozdobną. Mikropropagacja jest skutecznym narzędziem rozmnażania roślin ozdobnych na dużą skalę. Celem niniejszego badania była ocena wpływu różnych stężeĔ NAA i BA na mikropropagację i kwitnienie lisianthiusa in vitro. Używana pożywka hodowlana została wzbogacona za pomocą 0; 0,1; 0,2 i 2 mg L-1 NAA i BA. Przy powstawaniu eksplantów największa długość łodygi (2,07 cm na roślinę) była uzyskana na pożywce uzupełnionej o 0,1 mg L-1 BA (bez NAA). Maksymalna liczba łodyg (5,80 na roślinę) została wytworzona na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,2 mg L-1 NAA. Eksplanty pączków na pożywce hodowlanej zawierającej 0,2 mg L-1 NAA (bez BA) oraz 0,1 mg L-1 NAA wraz z 2 mg L-1 BA wytworzyły maksymalną liczbę węzłów (3,20 na roślinę). Największą liczbę korzeni (14,53 na roślinę) oraz największą długość korzenia (3,87 na roślinę) zaobserwowano na 0,2 mg L-1 NAA bez BA jak również 0,2 mg L-1 BA plus 0,2 mg L-1 NAA oraz 0,2 mg L-1 BA bez NAA. Eksplanty tworzyły kwiat na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,1 mg L-1 NAA bez przeniesienia kalusa. Kwiat był tworzony z kalusa na pożywce zawierającej 0,1 mg L-1 BA wraz z 2 mg L-1 NAA. Zregenerowane rośliny wykazały 98% przeżycie w szklarni podczas aklimatyzacji. Zaaklimatyzowane rośliny były morfologicznie podobne to swych roślin macierzystych.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 145-155
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bank genow 'in vitro' - stan obecny
    Autorzy:
    Zaklukiewicz, K
    Sekrecka, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/836124.pdf
    Data publikacji:
    1993
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    hodowla roslin
    ziemniaki
    kultury tkankowe
    hodowla in vitro
    banki genow
    odmiany roslin
    plant breeding
    potato
    tissue culture
    in vitro culture
    gene bank
    plant cultivar
    Źródło:
    Ziemniak Polski; 1993, 2; 28-36
    1425-4263
    Pojawia się w:
    Ziemniak Polski
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier
    Autorzy:
    Pilarek, M.
    Dąbkowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/185054.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    liquid perfluorochemical (fluorocarbon)
    liquid gas carrier
    mathematical modelling
    cell/tissue culture
    hybrid culture system
    ciecz perfluorochemiczna (fluorowęglowa)
    gaz ciekły
    modelowanie matematyczne
    hybrydowy system hodowli
    Opis:
    A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass.
    Źródło:
    Chemical and Process Engineering; 2016, 37, 1; 149-158
    0208-6425
    2300-1925
    Pojawia się w:
    Chemical and Process Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somaclonal variation in winter wheat [Triticum aestivum L.]: frequency, occurrence and inheritance
    Autorzy:
    Cheng, X Y
    Gao, M.W.
    Liang, Z.Q.
    Liu, G.Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044461.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    inheritance
    tissue culture
    Triticum aestivum
    occurrence
    in vitro
    winter wheat
    gene mutation
    plant breeding
    callus induction
    frequency
    somaclonal variation
    wheat
    embryo
    Opis:
    Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. General responses of regenerated plants were investigated and a total of 7142 R₂ spike lines from 1593 R₁ plants were assessed in the field for somaclonal variants in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic inheritance. From regenerated plantlets, 81% survived and 63% produced fertile plants. Forms with reduced plant height, length of spike and other morphological abnormalities were found in this progeny. Populations of R₁ plants were highly variable due mainly to the physiological disturbances resulting from the in vitro process. Overall somaclonal variation frequencies were 14.2% per plant basis and 5.3% per R₂ spike basis. The variants were similar in the three different R₂ generations with predominant variants being negative in plant height, maturity, awns, spike type and plant type. Both uniform R₂ variant families and spike lines were found in addition to the segregating variants which constituted the majority. On average, in a variant family or line, 18% and 14% of their component lines and plants were variants, respectively. Inheritability was demonstrated for the uniform variant families and spike lines as well as segregated variants. Of those 134 selections, about 70% were classified as inheritable. Both recessive and dominant gene mutations at one, two or three loci were evident in some variants as suggested by the segregating data.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 59-72
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture
    Autorzy:
    Parzymies, M.
    Pudelska, K.
    Poniewozik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/12683367.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pennisetum alopecuroides
    grass species
    ornamental grass
    shoot
    explant
    plant material
    tissue culture
    plant growth regulator
    micropropagation
    contamination
    plant regeneration
    silver
    nanoparticle
    disinfection
    Opis:
    Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm–3 Ag NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 127-135
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An improved procedure for in vivo and in vitro propagation of Buxus hyrcana, an ornamental shrub critically at risk of extinction
    Autorzy:
    Negahdar, N.
    Hashemabadi, D.
    Kaviani, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/80583.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ornamental shrub
    Buxus hyrcana
    endangered plant
    plant growth regulator
    rooting
    stem cutting
    tissue culture
    indole-3-butyric acid
    kinetin
    in vitro propagation
    in vivo
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
    Autorzy:
    Kozak, D.
    Parzymies, M.
    Świstowska, A.
    Marcinek, B.
    Ismael, B.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/12663898.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Brazilian jasmine
    Mandevilla sanderi
    ornamental plant
    flowering plant
    pot plant
    commercial plant
    plant growth
    plant development
    plant regeneration
    explant type
    multiplication
    decapitation
    defoliation
    in vitro culture
    tissue culture
    cultivation experiment
    Opis:
    Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
    Autorzy:
    Humphreys, M W
    Thomas, H M
    King, I P
    Morgan, W G
    Meredith, M R
    Harper, J A
    Humphreys, M O
    Bettany, A J E
    Dalton, S J
    James, A R
    Ougham, H J
    Thomas, H
    Powiązania:
    https://bibliotekanauki.pl/articles/2046675.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    RNA
    in situ
    tissue culture
    gene transfer
    chromosome segment
    anther culture
    androgenesis
    plant breeding
    introgression mapping
    fluorescence
    hybridization
    Lolium-Festuca complex
    drought resistance
    gene isolation
    tetraploid hybrid
    DNA
    meiosis
    grass
    chromosome behaviour
    Opis:
    Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 3; 273-284
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
    Autorzy:
    Marcinek, B.
    Parzymies, M.
    Poniewozik, M.
    Kozak, D.
    Durlak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/12307379.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Dahlia
    Dahlia x cultorum
    dahlia
    ornamental plant
    flowering plant
    tuberous plant
    tuberous root
    plant acclimatization
    growth regulator
    cytokinin
    gibberellic acid
    micropropagation
    tissue culture
    ex vitro method
    microcutting
    Opis:
    The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ estru etylowego kwasu p-hydroksybenzoesowego na zdolność wbudowywania 32p do kwasów nukleinowych komórki zwierzęcej - w hodowli tkankowej
    Vlijanie atilovogo afira p-gidrokibenaoinoi kisloty na cposobnost prisoedinenija 32 P k nukleinovym kislotam v aksplantacii zhivotnyh tkanei
    The influence of ethyl p-hydroxybenzoate on the incorporation of 32p into nucleic acids of animal cells in tissue culture
    Autorzy:
    Fitak, B.
    Lipinski, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/873670.pdf
    Data publikacji:
    1970
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    estry etylowe
    kwas p-hydroksybenzoesowy
    kwasy nukleinowe
    komorki zwierzece
    hodowla tkankowa
    zawartosc DNA
    zawartosc RNA
    ethyl ester
    p-hydroxybenzoic acid
    nucleic acid
    animal cell
    tissue culture
    DNA content
    RNA content
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 1970, 21, 2
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymers in medicine – direction of development
    Polimery w medycynie – kierunki rozwoju
    Autorzy:
    Dworak, Andrzej
    Utrata-Wesołek, Alicja
    Otulakowski, Łukasz
    Kasprów, Maciej
    Trzebicka, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/947555.pdf
    Data publikacji:
    2019
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Chemii Przemysłowej
    Tematy:
    polymeric materials in medicine
    intelligent materials
    drug carriers
    vascular stents
    vascular prostheses
    orthopedic implants
    tissue culture substrates
    antifouling layers
    materiały polimerowe w medycynie
    materiały inteligentne
    nośniki leków
    stenty
    protezy naczyniowe
    implanty ortopedyczne
    podłoża do hodowli tkanek
    warstwy przeciwporostowe
    Opis:
    The paper constitutes a brief and subjective review of polymeric materials in the contemporary health service. The range of applications of polymeric materials is discussed, special att ention being paid to such materials for the development of carriers of pharmaceutically active species, stents and vascular prostheses, amongst them to the application of „smart” materials for these purposes, layers and scaff olds for the growth of organs and tissues, antifouling layers. The authors try to turn the att ention of the reader to the research and intellectual eff orts necessary for the development of polymeric materials for the medicine, and conclude about the growing importance of such studies.
    Artykuł stanowi skrótowy, subiektywny przegląd materiałów polimerowych wykorzystywanych we współczesnej ochronie zdrowia. W pracy skupiono się na zastosowaniach materiałów polimerowych do konstrukcji nośników leków, stentów i protez naczyń, w tym także na użyciu polimerowych materiałów „inteligentnych”, implantów ortopedycznych oraz podłoży i rusztowań do hodowli komórek lub tkanek, a także warstw zapobiegających porastaniu wszczepionych konstruktów. Autorzy zwracają uwagę na znaczny wysiłek badawczy i intelektualny, niezbędny w procesie opracowania materiałów polimerowych dla medycyny, i na stale rosnące znaczenie takich badań.
    Źródło:
    Polimery; 2019, 64, 10; 645-655
    0032-2725
    Pojawia się w:
    Polimery
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    65 years of in vitro culture in Poland
    Autorzy:
    Zenkteler, M.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/58090.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    in vitro culture
    history
    plant tissue
    in vitro technology
    Polska
    Opis:
    This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wheat anther culture as affected by various cultural changes and supplements
    Autorzy:
    Simonson, R L
    Baenziger, P S
    Gustafson, V D
    Powiązania:
    https://bibliotekanauki.pl/articles/2046627.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cultural change
    supplement
    wheat
    silver nitrate
    Triticum aestivum
    anther culture
    cereal tissue
    Opis:
    With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 4; 381-392
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tissue engineering of bone: the role of osteoblasts in osteogenesis and peri-implant bone healing
    Autorzy:
    Wróbel, E.
    Witkowska-Zimny, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/284980.pdf
    Data publikacji:
    2013
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bone tissue engineering
    osteoblasts
    human bone-derived cells
    peri-implant bone healing
    osteogenesis
    cell culture
    Opis:
    Osteoblasts are cells of mesenchymal origin, which rebuild resorbed bone by synthesizing bone matrix proteins and by inducing bone matrix mineralization. Osteoblasts play a crucial role in creating and maintenance of healthy bone architecture, bone repair, and peri-implant bone healing (osseointegration). These bone-forming cells are also involved in regulation of osteoclasts function, and hence bone resorption in osteoclastogenesis process. We have presented our own studies on the subsequent stages of differentiation of Human Bone-Derived Cells (HBDCs) that could be a good candidate as an autogenous source for reconstruction and rebuilding of own patient's bone using tissue engineering methods. In this review we discussed the biology of osteoblasts, compared with the HBDCs cultures, under the influence of growth factors (FGF-2, TGF-ß, IGF, PDGF) and hormones (PTH, 1,25-dihydroxyvitamin D3, leptin). Our review is also focused on the participation of intercellular adhesion proteins (cadherins, claudins, connexin, 'OsteoMacs'), transcription factors (Cbfal, Msx-2, Osx, ATF4), and others molecules (RANKL, OPG, BMP2, lactofferin, PPARY) in modulating osteoblasts functions on the basis of current reports, throwing new light on the involvement of osteoblasts during osteogenesis and peri-implant bone healing.
    Źródło:
    Engineering of Biomaterials; 2013, 16, 119; 2-7
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimizing manufacturing conditions of polymer microspheres as cell carriers for modular tissue engineering
    Autorzy:
    Mielan, Bartosz
    Pamuła, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1844978.pdf
    Data publikacji:
    2020
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    modular tissue engineering
    microspheres
    cell culture
    oil-in-water emulsification
    poly(L-lactide-co-glycolide) (PLGA)
    Opis:
    Microspheres (MS) made of biostable polymer, namely polystyrene, have been used as substrates for cell culture enabling rapid cell expansion in dynamic conditions. However, due to non-resorbability, polystyrene (PS) MS when repopulated with cells cannot be directly used in tissue engineering. Our concept was to produce MS from resorbable polymer – poly(L-lactide- -co-glycolide) (PLGA) as a support for adherent cells, e.g. osteoblasts. We hypothesize that such MS can be applied to the injured site to act as cell carriers or as modules for modular tissue engineering (MTE). In this article, we present the results of optimizing the PLGA MS manufacturing conditions via oil-in-water emulsification. Due to such a technique, MS with the required size, size distribution and properties suitable for cell culturing can be obtained. Three parameters of the oil-in-water emulsification were examined: the stirring speed of a water phase during MS manufacturing, the surfactant concentration, i.e. poly(vinyl alcohol) (PVA) in a water phase and concentration of PLGA in dichloromethane (DCM) as an oil phase. The results proved that the 7.5% PLGA concentration in DCM solution as an oil phase, the 0.5-2% concentration of PVA solution as a water phase and the stirring speed of water phase of 1000 rpm provided MS with the 160 μm mean diameter, which is suitable for cell culture. Moreover, the developed sieving and cleaning procedures were efficient to collect MS with the mean diameter of 280 μm, the more coherent size distribution and the ability to sink in the cell culture medium. The presence on the bottom of cell culture wells is crucial for MTE.
    Źródło:
    Engineering of Biomaterials; 2020, 23, 156; 2-9
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Apoplastic markers of cell differentiation status during somatic embryogenesis in Arabidopsis thaliana
    Autorzy:
    Potocka, I.
    Sala, K.
    Kurczynska, E.U.
    Powiązania:
    https://bibliotekanauki.pl/articles/81256.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    somatic embryogenesis
    Arabidopsis thaliana
    plant tissue
    embryogenic culture
    immature zygotic embryo
    cotyledonary stage
    meristematic cell
    arabinogalactan protein
    lipid transfer protein
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Właściwości i kliniczne możliwości zastosowania ludzkich komórek nabłonka owodni (HAEC)
    Properties and clinical application of human amniotic epithelial cells (HAEC)
    Autorzy:
    Gawryluk, Arkadiusz
    Noszczyk, Bartłomiej
    Powiązania:
    https://bibliotekanauki.pl/articles/1030397.pdf
    Data publikacji:
    2015
    Wydawca:
    Medical Communications
    Tematy:
    amniotic epithelial cells
    amniotic membrane
    biological dressing
    cell culture
    tissue engineering
    hodowle komórkowe
    inżynieria tkankowa
    komórki nabłonka owodni
    opatrunki biologiczne
    owodnia
    Opis:
    In the contemporary medicine, undifferentiated progenitor cells of various origin and various degree of plasticity have become highly promising. Their most abundant, renewable and uncontroversial sources are placental tissues and umbilical blood. The only epithelial cells in this group come from the amnion which is used as a whole as an allogenic biological dressing. They have a range of unusual properties, such as the relative lack of histocompatibility antigens, plasticity (enabling their differentiation into a number of epithelial and mesenchymal cells) and the lack of neoplastic capacity. Amniotic epithelial cells are the only epithelial cells of the placenta. It is believed that they retain their progenitor (pluripotent) properties even in term pregnancies. This probably results from the fact that they omit the differentiation that accompanies gastrulation. Such features are typical of all placental cells which differ from amniotic epithelial cells only in their non-epithelial origin. In culture conditions, amniotic epithelial cells are characterized by a considerable plasticity: they can be stimulated to differentiate into adipocytes, chondrocytes, osteocytes, myocytes, cardiomyocytes, neurocytes, pancreatic cells and hepatocytes. To date, however, the attempts to direct their development towards the epidermis have not been successful. Obtaining multilayer epidermis in amniotic epithelial culture would be of considerable importance for tissue engineering of biological dressings. Amniotic membranes have been used for this purpose for many years, but because of their complex structure and metabolic requirements, they do not heal but dry up when applied to the wound. Some reports, however, indicate that the epithelium isolated from the amnion could be able to heal thus being suitable for allogenic grafts.
    Współczesna medycyna coraz większe nadzieje pokłada w niezróżnicowanych komórkach progenitorowych różnego pochodzenia i o różnym stopniu plastyczności. Ich najbardziej zasobnym, odnawialnym i niekontrowersyjnym źródłem wydają się tkanki łożyska i krew pępowinowa. Jedyne w tej grupie komórki nabłonkowe pochodzą z owodni, wykorzystywanej często w całości jako allogeniczny opatrunek biologiczny. Mają one szereg niezwykłych cech, takich jak względny brak ekspresji antygenów zgodności tkankowej, plastyczność (umożliwiająca różnicowanie w cały szereg komórek nabłonkowych i mezenchymalnych) oraz brak zdolności do nowotworzenia. Komórki nabłonka owodni są jedynymi nabłonkowymi komórkami łożyska. Uważa się, że nawet w donoszonej ciąży zachowują właściwości progenitorowe (pluripotencjalne). Wynika to prawdopodobnie z faktu, iż pomijają różnicowanie towarzyszące gastrulacji. Cechy te przejawiają zresztą wszystkie komórki łożyska, różniące się od komórek nabłonka owodni jedynie nienabłonkowym pochodzeniem. W hodowli komórki nabłonka owodni charakteryzują się dużą plastycznością: ulegają stymulacji do różnicowania w kierunku adypocytów, chondrocytów, osteocytów, miocytów, kardiomiocytów, neurocytów, komórek trzustki i hepatocytów. Dotychczas nie udało się jednak skierować ich rozwoju w kierunku naskórka. Uzyskanie nabłonka wielowarstwowego w hodowli komórek nabłonka owodni miałoby ogromne znaczenie dla inżynierii tkankowej opatrunków biologicznych. Błony owodniowe wykorzystywane są w tym celu od wielu lat, jednak wskutek złożonej struktury i wymagań metabolicznych nie ulegają wgajaniu – wysychają po położeniu na powierzchni rany. Niektóre badania wskazują natomiast, że nabłonek izolowany z owodni mógłby się wgajać, nadawałby się zatem do allogenicznych przeszczepów.
    Źródło:
    Current Gynecologic Oncology; 2015, 13, 2; 123-135
    2451-0750
    Pojawia się w:
    Current Gynecologic Oncology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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