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    Wyświetlanie 1-11 z 11
    Tytuł:
    HBx and SP1 upregulate DKK1 expression
    Autorzy:
    Peng, Hong
    Li, Yongguo
    Liu, Yunzhi
    Zhang, Jingnan
    Chen, Ke
    Huang, Ailong
    Tang, Hua
    Powiązania:
    https://bibliotekanauki.pl/articles/1038681.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    HCC
    HBV
    DKK1
    SP1
    Opis:
    Numerous evidences suggested that the hepatitis B virus (HBV) was recognized as an important factor in the development of hepatocellular carcinoma (HCC). Dickkopf-1 (DKK1) recently was reported to be involved in the progress of HCC. HBV may regulate DKK1 expression in hematoma carcinogenesis. Here, we demonstrated that HBV could regulate DKK1 promoter activity which resulted in upregulation of its mRNA and protein expression in several HBV existing cell lines, and HBx played a prominent role in this process. Transcription factor binding site search result showed that there is a SP1 site in DKK1 promoter region. Luciferase assay showed that overexpression of SP1 could increase DKK1 promoter activity in a dose dependent manner. Accordingly, siRNA inhibition of SP1 expression reduced DKK1 promoter activity and decreased the expression of DKK1 protein.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 1; 35-39
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The GC-box is critical for high level expression of the testis-specific Hsp70.2/Hst70 gene
    Autorzy:
    Widłak, Wiesława
    Vydra, Natalia
    Dudaladava, Volha
    Ścieglińska, Dorota
    Winiarski, Bolesław
    Krawczyk, Zdzisław
    Powiązania:
    https://bibliotekanauki.pl/articles/1041120.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Sp1
    GC-box
    regulation of gene expression
    spermatogenesis
    heat shock protein
    Opis:
    The Hsp70.2/Hst70 gene, which belongs to the 70 kDa heat-shock protein (HSP) family, is expressed specifically in primary spermatocytes and spermatids. The regulatory elements required for a high level of testis-specific expression of the gene are placed between the two major transcription start sites T1 and T2 (approximately 350 and 115 bp upstream of the starting ATG codon). Here we have shown that sequences proximal to the exon1/intron splicing site in the 5' untranslated region of the Hsp70.2/Hst70 gene, which include a highly conserved element called box B, are required for efficient expression of the chloramphenicol acetyltransferase reporter gene in testes of transgenic mice. However, in spite of the drastically reduced overall activity, the stage-specific expression pattern of the transgene was preserved after removal of these sequences. We have also shown that GC-box located downstream of the box B (approximately 210 bp upstream of the starting ATG codon) is indispensable for efficient expression of the Hsp70.2/Hst70 gene promoter in spermatogenic cells. The GC-box specifically binds proteins present in nuclear extracts from testes (putatively Sp1-like factors). A change in the pattern of such GC-box-interacting factors corresponds to activation of the Hsp70.2/Hst70 gene, confirming the importance of this regulatory element.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 1; 107-112
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena mikroflory w powietrzu chłodziarek w gospodarstwach domowych
    Estimate of the air microflora of the refrigerators from houshold
    Autorzy:
    Kawecka, W.
    Wrońska, A.
    Tarkowska, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/228356.pdf
    Data publikacji:
    2012
    Wydawca:
    Wyższa Szkoła Menedżerska w Warszawie
    Tematy:
    zanieczyszczenia mikrobiologiczne żywności
    chłodzenie
    Listeria monocytogenes
    Bacillus sp1
    grzyby strzępkowe
    microbiological contamination
    filamentous fungi
    Opis:
    Przechowywana w warunkach chłodniczych żywność stanowi dobrą pożywkę dla rozwoju mikroorganizmów. Celem przeprowadzonych badań była ocena stopnia zanieczyszczeń mikrobiologicznych powietrza w urządzeniach chłodniczych w gospodarstwach domowych. W prowadzonych badaniach wykazano obecność zarodników bakterii saprofitycznych oraz chorobotwórczych szczepów Listeria monocytogenes oraz grzybów potencjalnie toksynotwórczych z rodzajów Aspergillus i Penicillium.
    Food presents good source in cooling conditions for development of microorganism stored. The aim of investigation there was the opinion of microbiological contamination of the air of refrigerators from household. Presence of presence exert in composition microflora pathogenic spores Listeria monocytogenes, Bacillus sp. and spores potential toxinogenic filamentous fungi of genres Aspergillus and Penicillium.
    Źródło:
    Postępy Techniki Przetwórstwa Spożywczego; 2012, 1; 43-46
    0867-793X
    2719-3691
    Pojawia się w:
    Postępy Techniki Przetwórstwa Spożywczego
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biological removal of nickel (II) by Bacillus sp. KL1 in different conditions: optimization by Taguchi statistical approach
    Autorzy:
    Taran, M.
    Sisakhtnezhad, S.
    Azin, T
    Powiązania:
    https://bibliotekanauki.pl/articles/777958.pdf
    Data publikacji:
    2015
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    Nickel
    Bioremediation
    Garbage leachate
    Bacillus sp KL1
    Taguchi method
    Opis:
    Bioremediation is the removal of heavy-metals such as nickel (Ni) using microorganisms and has been considered as an important field in the biotechnology. Isolation and characterization of microorganisms exhibiting bioremediation activities and their optimization to treat polluted wastewaters is a vital and difficult task in remediation technologies. In this study, investigation was carried out to isolate Ni (II) remediating microbial strains from soils contaminated with municipal solid waste leachate. Furthermore, Taguchi design of experiments were used to evaluate the influence of concentration, pH, temperature, and time on bioremediation of Ni (II) using isolated bacteria. This study concluded that Bacillus sp. KL1 is a Ni (II)-resistant strain and had Ni (II) bioremediation activity. The highest bioremediation of Ni (II) was observed as 55.06% after 24 h at 30ºC, pH 7, and 100 ppm concentration. Moreover, it was also observed that concentration is the most effective factor in the bioremediation process. In conclusion, we have demonstrated that bacteria isolated from soils contaminated with garbage leachate have the Bacillus sp. KL1 bacteria which can efficiently uptake and eliminate Ni (II) from contaminated sites and thus makes it possible to treat heavy-metal containing wastewaters in industry by using this microorganism at optimized conditions.
    Źródło:
    Polish Journal of Chemical Technology; 2015, 17, 3; 29-32
    1509-8117
    1899-4741
    Pojawia się w:
    Polish Journal of Chemical Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    p53-dependent suppression of the human calcyclin gene (S100A6): the role of Sp1 and of NFκB
    Autorzy:
    Króliczak, Weronika
    Pietrzak, Maciej
    Puzianowska-Kuznicka, Monika
    Powiązania:
    https://bibliotekanauki.pl/articles/1040715.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    gene suppression
    wild type and mutant p53
    Sp1
    calcyclin gene (S100A6)
    NFκB
    Opis:
    Calcyclin (S100A6) is believed to participate in cell cycle control. It was, however, unclear if its expression depends on p53, a key regulator of apoptosis and cell cycle. We therefore performed transcription regulation assays in HeLa cells and found that wild type p53 suppressed the S100A6 promoter up to 12-fold in a dose-dependent manner. In contrast, the well-characterized V143A, R175H, R249S, and L344A p53 mutants cloned from human cancers suppressed this promoter with a 6 to 9-fold lower efficiency. All the sites mediating the p53-dependent suppression were contained in the -167 to +134 fragment of the S100A6 promoter. Separate overexpression of either Sp1 or of NFκB only partially counteracted the p53 inhibitory effect on the S100A6 promoter, while simultaneous overexpression of both these transactivators resulted in a complete abolishment of the p53 inhibitory effect on this promoter. Sp1 and NFκB binding to the probes resembling their putative binding sites present in the S100A6 promoter was decreased in the presence of wild type p53. We propose that the suppression of S100A6 is yet another mechanism by which p53 inhibits proliferation. Insufficient suppression of this gene by p53 mutants could well be responsible for calcyclin overexpression and cell cycle deregulation observed in cancer tissues.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 3; 559-570
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sp1 mediates phorbol ester (PMA)-induced expression of membrane-bound guanylyl cyclase GC-A in human monocytic THP-1 cells
    Autorzy:
    Mitkiewicz, Małgorzata
    Bac, Bernadeta
    Kuropatwa, Marianna
    Kurowska, Ewa
    Matuszyk, Janusz
    Siednienko, Jakub
    Powiązania:
    https://bibliotekanauki.pl/articles/1038369.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    membrane-bound guanylyl cyclase type A
    phorbol 12-myristate 13-acetate
    human monocytic cell line THP-1
    protein kinases
    Sp1 transcription factor
    Opis:
    Cyclic guanosine monophosphate (cGMP) is synthesized by two types of enzymes: particulate (membrane-bound) guanylyl cyclases (pGCs) and soluble (cytosolic) guanylyl cyclases (sGCs). sGCs are primarily activated by binding of nitric oxide to their prosthetic heme group while pGCs are activated by binding of peptide ligands to their extracellular domains. One of them, pGC type A (GC-A) is activated by atrial and brain natriuretic peptides (ANP and BNP, respectively). Human monocytes isolated from peripheral blood mononuclear cells have been found to display sGC expression without concomitant expression of GC-A. However, GC-A activity appears in monocytes under certain conditions but a molecular mechanism of GC-A expression is still poorly understood. In this report we show that phorbol ester (PMA) induces transcription of a gene encoding GC-A in human monocytic THP-1 cells. Moreover, we find that PMA-treated THP-1 cells raise cGMP content following treatment with ANP. Studies using pharmacological inhibitors of protein kinases suggest involvement of protein kinase C (PKC), mitogen extracellular kinases (MEK1/2), and extracellular signal-regulated kinases (ERK1/2) in PMA-induced expression of the GC-A encoding gene in THP-1 cells. Finally, we show that PMA stimulates binding of Sp1 transcription factor to GC-rich DNA sequences and mithramycin A (a selective Sp1 inhibitor) inhibits expression of the GC-A mRNA in PMA-treated THP-1 cells. Taken together, our findings suggest that the PMA-stimulated PKC and MEK/ERK signaling pathways induce Sp1-mediated transcription of the GC-A encoding gene in human monocytic THP-1 cells.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 3; 409-414
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometry identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus)
    Autorzy:
    Polcyn, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040683.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    immunoblot screening
    membrane-bound nitrate reductase [EC 1.7.99.4]
    enzyme purification
    Bradyrhizobium sp. (Lupinus)
    rhizobia
    mass spectrometry sequencing
    Opis:
    Respiratory nitrate reductase (NR) from Bradyrhizobium sp. (Lupinus) USDA 3045 has biochemical properties of the membrane-bound NR type. However, in the completely sequenced rhizobium genomes only genes for the periplasmic type of dissimilatory NR were found. Therefore purification and identification of the enzyme by tandem mass spectrometry (MS/MS) was undertaken. MS/MS spectra representing 149 unique tryptic peptides derived from purified 137-kDa subunit matched the NCBInr-deposited NarG sequences. MS/MS sequencing of two other SDS/PAGE bands (65- and 59-kDa) identified them as derivatives of the NarH-type protein. Applying additional validation criteria, 73% of the sequence of the NarG subunit (902 aa) and 52% of NarH sequence (266 aa) was assembled (UniProt KB acc. no. P85097 and P85098). This is the first unambiguous identification of an active NarGH-like NR in rhizobia. Moreover, arguments are provided here for the existence of a functional enzyme of this type also among other rhizobial species, basing on immunoblot screening and the presence of membrane-associated NR-active electrophoretic forms.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 753-760
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nitrate-related down-regulation of respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus)
    Autorzy:
    Polcyn, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040684.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    multiple enzyme forms
    membrane-bound nitrate reductase [EC 1.7.99.4]
    down-regulation
    nitrate and nitrite induction
    stability of subunits
    Bradyrhizobium sp. (Lupinus)
    Opis:
    Previously, we showed that anaerobic induction of respiratory nitrate reductase (NR) activity in Bradyrhizobium sp. (Lupinus) USDA 3045 is strongly enhanced by nitrate or nitrite through de novo synthesis. Here, multiple NR-active soluble forms, ranging from 75 kDa to 190 kDa, were observed under anaerobic conditions. Electrophoretic activity band patterns differed depending on the level and the type of the N oxyanion added. The intensity of the membrane-bound NR activity band of 230 kDa changed with time along with consumption of 2 mM nitrate. It was associated with a parallel 5-fold increase and then 2-fold reduction in the amount of membrane-bound NR protein. In contrast, on 4 mM nitrate, the level of NR protein was much more stable, apparently due to slower nitrate depletion. Moreover, in cells anaerobically grown without nitrate addition, a 42-kDa derivative of NR degradation was immunodetected, which was not observed if nitrate was present in the medium. These findings suggest that the amount of the respiratory NR protein could be negatively regulated by endogenous proteases in relation to the level of nitrate available. It seems, therefore, that multiple native forms might be not different isoenzymes but immature complexes or derivatives of the enzyme protein turnover. This report adds to a modest list of bacterial enzymes apparently regulated by proteolysis, such as GS, MurAA, EnvA, GdhA, and MetA.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 761-766
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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