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    Wyświetlanie 1-3 z 3
    Tytuł:
    Isolation and partial purification of anticoagulant fractions from the venom of the Iranian snake Echis carinatus
    Autorzy:
    Babaie, Mahdi
    Zolfagharian, Hossein
    Salmanizadeh, Hossein
    Mirakabadi, Abbas
    Alizadeh, Hafezeh
    Powiązania:
    https://bibliotekanauki.pl/articles/1039597.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    anticoagulant factor
    snake venom
    Iranian Echis carinatus
    chromatography
    Opis:
    Many snake venoms comprise different factors, which can either promote or inhibit the blood coagulation pathway. Coagulation disorders and hemorrhage belong to the most prominent features of bites of the many vipers. A number of these factors interact with components of the human blood coagulation. This study is focused on the effect of Echis carinatus snake venom on blood coagulation pathway. Anticoagulant factors were purified from the Iranian Echis carinatus venom by two steps: gel filtration (Sephadex G-75) and ion-exchange (DEAE-Sephadex) chromatography, in order to study the anticoagulant effect of crude venom and their fractions. The prothrombin time was estimated on human plasma for each fraction. Our results showed that protrombin time value was increase from 13.4 s to 170 s for F2C and to 280 s for F2D. Our study showed that these fractions of the venom delay the prothrombine time and thus can be considered as anticoagulant factors. They were shown to exhibit proteolytic activity. The molecular weights of these anticoagulants (F2C, F2D) were estimated by SDS/PAGE electrophoresis. F2C comprises two protein bands with molecular weights of 50 and 79 kDa and F2D a single band with a molecular weight of 42 kDa.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 1; 17-20
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Anti-Snake venom Activities of the leaf extracts of Asystasia gangetica (L) and Newbouldia leavis (p. Beauv)
    Autorzy:
    Enenebeaku, C. K.
    Umerie, S. C.
    Nwankwo, M. U.
    Enenebeaku, U. E.
    Powiązania:
    https://bibliotekanauki.pl/articles/1112887.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Asystasia gangetica
    Medicinal plants
    Naja melanoleuca
    Newbouldia leavis
    Snake Venom
    Opis:
    The leaf extracts of two medicinal plants Asystasia gangetica (L) and Newboulia leavis (p. Beauv) were assessed for anti-snake venom activity in vivo using mice. The result of the phytochemical analysis revealed that the two plants contain flavonoids, glycosides, saponins, tannins and alkaloids. The methanolic extracts of the two plants A. gangetica (L) and N. leavis (p. Beauv) significantly (p< 0.05) neutralized the Naja melanoleuca venom-induced lethality activity in the mice. The extracts (flavonoids, tannins and saponins) of the two plants also showed significant (p<0.05) neutralization of the venom–induced lethality activity in mice. The work confirmed that Asystasia gangetica and Newbouldia leavis possess significant anti-venom activity and can therefore be used in the treatment of snake bites.
    Źródło:
    World News of Natural Sciences; 2018, 16; 33-41
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Is coagulopathy a common consequence of a Vipera berus bite? A retrospective single centre study
    Autorzy:
    Dylag-Trojanowska, K.E.
    Hodorowicz-Zaniewska, D.
    Zybaczyńska, J.
    Szczepanik, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081944.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    thrombocytopenia
    coagulopathy
    venom
    antivenom
    envenomation
    Vipera berus bite
    snake
    snakebite
    Opis:
    Introduction. The Vipera berus (common viper) is the only species of venomous snake found in Poland. The aim of this study was to determine to what extent coagulopathy has occurred as a post-bite complication. Materials and method. The medical records of 138 adult patients in the Sucha Beskidzka hospital with the diagnosis of snake bite between 2001–2014 were retrospectively analyzed. Demographic, clinical and laboratory data were collected. Antivenom was given to all patients, except one, with snake bites, but laboratory test were taken prior to administration of antivenom. Obtained results were compared to those of the control group, composed of 176 adults hospitalized in the same period for an elective laparoscopic cholecystectomy. Results. The mean platelet count in the study group was 239.94×109/L (SD=56.56) and 248.77×109/L (SD=57.82) in the control group. In 98% of the study group and 100% of the control group the platelet value (PLT) fell within laboratory norms (130 to 420×109/L), 2 patients after snake bites had a PLT lower than normal, and thrombocytosis was not observed in either group. A reference range of 0.85–1.3 INR below normal was found in 2 patients in the study group and 15 from the control group, while values above the normal range were found in 8 patients (6.7%) from the snake bite group and no patients from the control group. In the study group, the INR ranged from 0.78–1.43 with a mean of 1.046 (SD=0.14), while in the control group the range was from 0.79–1.28, with a mean of 0.95 (SD=0.08). There was a significant difference in INR between the study and control groups (p<0.0001). Conclusions. The bite from V. berus does not result in severe thrombocytopenia, with only a moderate increase in INR values observed in about 7% of patients.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 630-634
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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