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    Tytuł:
    Optimization of P and K fertilizer recommendation for faba bean in Ethiopia: the case for Sekela District
    Autorzy:
    Getu, Abebe
    Gashu, Kindu
    Mengie, Yechale
    Agumas, Birhanu
    Abewa, Anteneh
    Alemayehu, Amlaku
    Powiązania:
    https://bibliotekanauki.pl/articles/1031495.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Sekela
    Vicia faba
    faba bean
    fertilizer
    phosphorus
    rhizobia inoculation
    Opis:
    Ethiopian soils are recently reported to exhibit multi-nutrient deficiency. Legume crops can satisfy their nitrogen (N) demand through biological N fixation. However, other nutrients should be supplied in optimal amount in the form of fertilizers. Thus, this study was conducted to evaluate the response of faba bean to different levels of phosphorus (P) and potassium (K) fertilizers. The study was conducted in Sekela district of West Gojam Zone of Amhara region from 2014 to 2015. Four levels of K (0, 12, 24 and 36 K2O kg ha-1) and five levels of P (0, 23, 46, 69 and 92 kg P2O5 ha-1) in the first year and four levels of P (0, 23, 46 and 69 kg P2O5 ha-1) in the second and third years were combined in incomplete factorial arrangement with a satellite treatment comprising S, Zn, Mg and B. Except the control treatment (non-fertilized), all the rest treatments were inoculated with FB-4 in the first year and EAL-110 rhizobia bacteria in the second and third experimental years. The treatments were laid in a randomized complete block design with three replications. P, K, S and Mg fertilizers were applied as basal application, while, the micronutrients Zn and B were applied as foliar application 45 days after planting. Significant (P<0.05) yield response to the addition of P and K fertilizers was recorded in two experimental years. In the first experimental year, the highest grain of 3.2 t ha-1 was obtained from application of 92/24 P2O5/K2O kg ha-1 statistically at par with 46/0 P2O5/K2O kg ha-1. While, in the second experimental year, the maximum grain yield of 4.3 t ha-1 was obtained from application of 46/0 P2O5/K2O kg ha-1. The partial budget analysis result showed that the maximum marginal rates of return of 611 and 463.5% and maximum net economic returns of 1768.1$ and 2975.2$ were obtained from application of 46 kg P2O5 ha-1 in the first and second experimental years, respectively. Thus, the combined use of the rhizobia strains FB-4 and EAL-110 with 46 kg P2O5 ha-1 is recommended to improve productivity and economic return of faba bean production in Sekela district and similar agro-ecologies.
    Źródło:
    World Scientific News; 2020, 142; 169-179
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Diversity and numbers of root-nodule bacteria [Rhizobia] in Polish soils
    Autorzy:
    Martyniuk, S
    Oron, J.
    Martyniuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/57868.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    legume
    Rhizobia
    diversity
    soil
    soil property
    bacteria
    number
    root nodule bacteria
    Polish soil
    Opis:
    Using a sand pouch-plant infection method, populations of several species of root-nodule bacteria (rhizobia) were enumerated in eighty soils collected throughout Poland. Rhizobium leguminosarum bv. viciae (symbionts of pea, faba bean, vetch) and R. leguminosarum bv. trifolii (symbionts of clover) were detected in 77 and 76 soils, respectively. Most of these soils contained moderate and high numbers of these species of the rhizobia. Symbionts of beans, R. leguminosarum bv. phaseoli, were assessed in 76 soils; of this number 15 soils had no detectable populations of bean rhizobia and in 40 soils high or moderate numbers of these bacteria were found. Bradyrhizobium sp. (Lupinus), root-nodule bacteria of lupine and serradella, were absent in 19 soils, out of 80 tested, and 34 soils were colonised by high or moderate populations of bradyrhizobia. Sinorhizobium meliloti, rhizobia nodulating alfalfa, were sparse in the examined soils; with 56 soil containing no detectable numbers of S. meliloti and only 6 soils harbouring high or moderate populations of this species. The estimated numbers of the rhizobia in the studied soils were also related to some physical and chemical properties of these soils.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2005, 74, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic characterization of some rhizobial isolates from various legumes
    Autorzy:
    El-Ghany, E.M.A.
    Eissa, R.A.
    Fahmi, A.I.
    Nagaty, H.H.
    El-Zanaty, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2097126.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    rhizobia
    morphological
    API 20E and API 20NE
    16S rRNA
    phylogenetic
    Opis:
    In the last few decades, there has been a growing interest in environmentally friendly sustainable agricultural practices, thus increasing the role of biofertilizers such as rhizobia, which can decrease the need for chemical fertilizers, reduce adverse environmental effects, and help to save money. Therefore, information on the distribution and genetic variation of native rhizobial isolates would aid in selecting novel rhizobial strains that could be developed and used as biofertilizers in legume production. This research was conducted to characterize 24 rhizobial isolates from five legumes on morphological, biochemical, and molecular aspects and determine the phylogenetic relationships among them. Rhizobial isolates were obtained from five Egyptian legumes: faba bean, lentil, pea, clover, and soybean. Morphological characterization classified the isolates into fast and slow growers. Biochemical characterization using API 20E and API 20NE systems showed a large diversity, which may reflect their adaptation in different environments. Moreover, molecular detection of the 16S rRNA gene enabled to characterize 19 of them to the species level. Rhizobial isolates from pea, faba bean, clover, and lentil were identified as Rhizobium leguminosarum and those from soybean were identified as Bradyrhizobium japonicum. These data reflected a narrow diversity of rhizobial species in Egypt. A phylogenetic analysis of the 19 isolates confirmed that B. japonicum isolates were divergent from all other isolates. Furthermore, the phylogram revealed that each group of isolates that originated from the root nodule of a certain legume formed a separate subcluster. The obtained data suggested a narrow range of interspecies variations, which is consistent with the idea of the presence of biovars among the species.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 3; 179-191
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The potential and pitfalls of exploiting nitrogen fixing bacteria in agricultural soils as a substitute for inorganic fertiliser
    Autorzy:
    Cummings, S. P.
    Humphry, D. R.
    Santos, S. R.
    Andrews, M.
    James, E. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363190.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    szczepionka
    żyzność gleby
    rolnictwo
    taksonomia
    Rhizobium
    Rhizobial inoculants
    soil fertility
    agriculture
    taxonomy
    rhizobia
    Opis:
    Nitrogen fixing bacteria have been used for centuries to improve the fertility of agricultural soils. Since the introduction of inorganic nitrogen (N) fertiliser that provides a reliable boost to crop yields whilst reducing land and labour requirements, the use of biological nitrogen fixation has been in decline. Recently, concerns have been expressed about the sustainability of inorganic N fertiliser application, however, there remain doubts about whether N2 fixing bacteria alone can provide agriculture with sufficient fixed N to feed a burgeoning global population. In this paper we review the current state of our knowledge regarding those diazotrophic bacteria that have a role to play in agriculture. We focus on our current areas of research, particularly, the importance of understanding the classification and mechanism of action of N2 fixing bacteria that are used in agricultural soils. We discuss the applications of N2 fixing bacteria that illustrate their potential to provide sustainable N, particularly focussing on Australian and South American agricultural systems where these bacteria are widely exploited to maintain soil fertility. We also identify problems with the use of bacteria as inoculants, including ineffective inoculation due to poor quality preparation, the use of appropriate isolates and issues with sustainability. We review the outlook for biological N fixation highlighting how molecular biology may enable the expression of N fixation in non-leguminous crops.
    Źródło:
    Environmental Biotechnology; 2006, 2, 1; 1-10
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of PRP Sol on microbial and biochemical soil properties
    Oddziaływanie PRP Sol na mikrobiologiczne i biochemiczne właściwości gleby
    Autorzy:
    Martyniuk, S.
    Kozieł, M.
    Jończyk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/336604.pdf
    Data publikacji:
    2014
    Wydawca:
    Sieć Badawcza Łukasiewicz - Przemysłowy Instytut Maszyn Rolniczych
    Tematy:
    soil microorganism
    Azotobacter
    rhizobia
    enzymes
    PRP Sol
    soil improver
    mikroorganizm glebowy
    rizobia
    enzym
    polepszacz glebowy
    Opis:
    The aim of this work was to assess some microbial and enzymatic parameters in soil under winter wheat, maize and spring barley treated with PRP Sol in comparison to soil fertilized with NPK. Estimates of: total numbers of bacteria and fungi, numbers of Azotobacter spp., MPN of rhizobia, numbers of spores of AM fungi, glomalin content, as well as phosphatases activities in soil under winter wheat, corn and spring barley treated with PRP Sol generally did not differ significantly from those found in soil fertilized with NPK.
    Celem badań było porównanie wpływu PRP Sol i nawożenia NPK na wybrane grupy mikroorganizmów i aktywność enzymów w glebie pod uprawą zbóż (pszenica ozima, kukurydza, jęczmień jary). Wartości następujących parametrów: ogólna liczebność bakterii i grzybów, liczebność Azotobacter spp., MPN rizobiów, liczebność spor grzybów mikoryzowych (AM), zawartość glomalin oraz aktywność fosfatazy kwaśniej i zasadowej w glebie pod ww. roślinami nawożonymi PRP Sol nie różniły się na ogół istotnie od wartości tych parametrów w glebie nawożonej NPK.
    Źródło:
    Journal of Research and Applications in Agricultural Engineering; 2014, 59, 4; 37-39
    1642-686X
    2719-423X
    Pojawia się w:
    Journal of Research and Applications in Agricultural Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Testing Rhizobia for Natural and Anthropogenic Saline Soils and Subsoils
    Autorzy:
    Skipin, Leonid
    Gaevaya, Elena
    Tarasova, Svetlana
    Powiązania:
    https://bibliotekanauki.pl/articles/1838399.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    Rhizobia
    nodule bacteria
    colony
    chemistry of salinity
    degree of salinity
    osmotic pressure
    strain
    pH of the medium
    Opis:
    The area of naturally saline lands in Russia is approximately 30 million hectares. Currently, the growth of anthropogenic saline areas is associated with the extraction of minerals (agricultural ore) and the formation of drilling pits when drilling wells for the exploration and extraction of hydrocarbons. The number of drilling pits with high salt content in the Khanty-Mansi Autonomous Okrug is about 3 thousand. About the same number of them is found in the Yamalo-Nenets Autonomous Okrug. In the south of the Tyumen region, their number is increasing in the Uvat district. Reclamation of these areas includes the technical and biological phases of reclamation. The biological stage involves the use of phytomeliorant crops. Inclusion of the legume component in phytomeliorants implies its joint use with a preparation of active strain of nodule bacteria. The aim of the research was to identify the most salt-tolerant strain of lupine nodule bacteria under different chemistry and degree of salinity of the environment. In order to test lupine rhizobia for salt tolerance and salinity chemistry, laboratory experiments were set up in petri dishes. Lupine rhizobia strains 363a, 367a, and 375a were taken directly from the rhizotorphin preparations, and the degree of dilution was brought to a certain titer corresponding to about 80 colonies per Petri dish. The presented results of the experiments showed that the colonies of lupine rhizobia react extremely negatively to the concentration of salts in the nutrient medium of 0.3% or higher. This is true for both neutral and sodium salinity. Out of the three strains of rhizobia studied, sample 367a was the most salt tolerant. Strains 363a and 375a were less resistant to salinization. For the first time, strains of lupine rhizobia were tested for salt tolerance in relation to different chemistry and degree of salinity identical to anthropogenic soils and saline soils.
    Źródło:
    Journal of Ecological Engineering; 2021, 22, 5; 139-142
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biologiczne wiązanie N2, bakterie symbiotyczne roślin bobowatych w glebach Polski i oszacowywanie ich liczebności
    Biological fixation of N2, symbiotic bacteria of legumes in Polish soils and estimation of their numbers
    Autorzy:
    Martyniuk, Stefan
    Powiązania:
    https://bibliotekanauki.pl/articles/2147975.pdf
    Data publikacji:
    2019-09-30
    Wydawca:
    Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
    Tematy:
    biological N2 fixation
    legumes
    rhizobia
    number
    soil
    estimation
    biologiczne wiązanie N2
    rośliny bobowate
    ryzobia
    liczebność
    gleba
    oszacowanie
    Opis:
    Plonowanie roślin motylkowatych, podobnie jakwszystkich innych roślin uprawnych, zależy od wielu czynnikówśrodowiskowych, agrotechnicznych i biologicznych.Omawiana grupa roślin wyróżnia się jednak tym, że ichrozwój i plonowanie zależy także w znacznym stopniu odobecności w glebie specyficznej grupy bakterii, tj. bakteriiwykazujących zdolność do biologicznego wiązania, czyliprzyswajania azotu cząsteczkowego (atmosferycznego)w symbiozie z korzeniami tych roślin. Badania wykazały, żebakterie symbiotyczne (ryzobia) koniczyny, grochu i bobikuwystępują dość powszechnie w glebach Polski, nawet w glebach,na których od wielu lat nie uprawiano wymienionychroślin. Bakterii tych nie stwierdzono tylko w około 5% gleb(na 80 badanych) i były to gleby lekkie, silnie zakwaszone(pH(KCl) < 4,5). Bakterii symbiotycznych łubinu i fasoli nie wykrytow około 25% badanych gleb. Symbionty łubinu są najliczniejszew glebach średnio zwięzłych i w glebach lekkicho odczynie lekko kwaśnym lub kwaśnym, czyli w glebach najczęściejobsiewanych tą rośliną. W większości naszych glebbrak jest bakterii symbiotycznych lucerny i soi lub liczebnośćtych bakterii jest mała, z wyjątkiem gleb obsiewanych tymiroślinami.W zawiązku z brakiem selektywnych podłoży do oznaczanialiczebności bakterii symbiotycznych w glebach oszacowywaniepopulacji tych bakterii przeprowadza się metodamipośrednimi, w oparciu o wyniki biotestów z siewkami roślinbobowatych zaszczepionych wzrastającymi rozcieńczeniamibadanej gleby. W pracy opisano dokładnie różne sposobyprzeprowadzania biotestów oraz określania liczebności ryzobiów.
    Yields of legumionous plants, as well as other arable crops, depend on numerous environmental, agro-technical and biological factors. In the case of legumes colonization of soils with a specific group of symbiotic bacteria which fix atmospheric nitrogen is also an important factor influencing the development and yields of these crops. Results of our survey have shown that symbiotic bacteria (rhizobia) of clover, faba bean and pea commonly occur in soils of Poland, even in soil not planted to these crops for a long time. These bacteria have not been detected only in about 5% of soils (out of 80 examined) and these were light and strongly acidic (pHKCl < 4.5) soils. Symbiotic bacteria of lupine and field bean were not found in about 25% of the studied Polish soils. Symbionts of lupine were most numerous in light- and medium-textured soils with slightly acidic pH. In the majority of Polish soils symbiotic bacteria of alfalfa and soybean were not detected or their numbers were low, except those soil on which these plants were cropped recently.Due to the lack of selective media to count root nodule bacteria in soils and other materials (inoculants) populations of these bacteria are usually assessed by indirect methods based on biotests with legume seedling inoculated with increasing dilutions of a studied soil. In this article different biotests and methods of estimation of rhizobial numbers are described.
    Źródło:
    Polish Journal of Agronomy; 2019, 38; 52-65
    2081-2787
    Pojawia się w:
    Polish Journal of Agronomy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometry identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus)
    Autorzy:
    Polcyn, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040683.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    immunoblot screening
    membrane-bound nitrate reductase [EC 1.7.99.4]
    enzyme purification
    Bradyrhizobium sp. (Lupinus)
    rhizobia
    mass spectrometry sequencing
    Opis:
    Respiratory nitrate reductase (NR) from Bradyrhizobium sp. (Lupinus) USDA 3045 has biochemical properties of the membrane-bound NR type. However, in the completely sequenced rhizobium genomes only genes for the periplasmic type of dissimilatory NR were found. Therefore purification and identification of the enzyme by tandem mass spectrometry (MS/MS) was undertaken. MS/MS spectra representing 149 unique tryptic peptides derived from purified 137-kDa subunit matched the NCBInr-deposited NarG sequences. MS/MS sequencing of two other SDS/PAGE bands (65- and 59-kDa) identified them as derivatives of the NarH-type protein. Applying additional validation criteria, 73% of the sequence of the NarG subunit (902 aa) and 52% of NarH sequence (266 aa) was assembled (UniProt KB acc. no. P85097 and P85098). This is the first unambiguous identification of an active NarGH-like NR in rhizobia. Moreover, arguments are provided here for the existence of a functional enzyme of this type also among other rhizobial species, basing on immunoblot screening and the presence of membrane-associated NR-active electrophoretic forms.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 753-760
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structural enzymology at the legume-microbe interface: S-adenosyl-L-homocysteine hydrolase of rhizobia
    Autorzy:
    Manszewski, T.
    Singh, K.
    Imiolczyk, B.
    Jaskolski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80810.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    S-adenosyl-L-homocysteine hydrolase
    rhizobia
    biological methylation
    methyl group
    enzymatic hydrolysis
    enzymatic process
    crystal structure
    phylogenetic analysis
    biochemical analysis
    legume plant
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of arbuscular mycorrhizal fungi and nodules in the roots of twelve legume species in South-Western Saudi Arabia
    Autorzy:
    Mahdhi, M.
    Tounekti, T.
    Khemira, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/11862057.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Saudi Arabia
    plant cultivation
    legume plant
    mycorrhizal fungi
    arbuscular mycorrhizal fungi
    Rhizobia
    root nodule
    fungi occurrence
    soil microbial biomass
    soil fertility
    arid land
    Opis:
    The tripartite associations of arbuscular mycorrhizal fungi (AMF), rhizobia and legumes play a vital role in preserving and even restoring fertility of poor and eroded soils. The present study attempted to quantify relationship between legumes and symbiotic microorganisms (rhizobia and mycorrhizal fungi) by describing the mycorrhizal status and the occurrence of nodualtion of legumes growing in different areas of Jazan. The effect of legume species on soil microbial biomass was also investigated. Mycorrhizal and nodulation intensity varied greatly between legume species. The higher number of nodules (14 nodules per plant) and mycorrhization intensity (54%) were registered in root of Argyrolobium arabicum. Rhizosphere soils of all legume species harbored higher AMF fungal spores than bulk soils. Our results suggest also a significant effect of legumes species on soil microbial biomass. Thus, legume species investigated in this study are potentially useful for replanting and soil protection of most degraded regions of Saudi Arabia.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 4; 53-60
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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