Temat: Real-Time qPCR - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Quantitative Dynamics of Chosen Bacteria Phylla in Wastewater Treatment Plants Excess Sludge After Ozone Treatment
Autorzy:: Jurczyk, Łukasz
Koc-Jurczyk, Justyna
Balawejder, Maciej
Powiązania:: https://bibliotekanauki.pl/articles/123123.pdf
Data publikacji:: 2019
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: ozone treatment
excessive sludge
Real-Time qPCR
sludge microbial community
microbial quantitative dynamics
Opis:: Among the waste produced by municipal wastewater treatment plants, the largest technological problem is caused by the excessive sludge, the management of which consumes almost half of the costs of the entire installation. Regardless of the final disposal route, which may be, for example, reclamation of degraded areas, the the sludge derived from a wastewater treatment plant should be firstly subjected to a series of processes aimed at: reducing its volume, improving selected physical properties, recovering energy, rot preventing (stabilization) or eliminating epidemiological threats (hygenizing). These goals could be implemented using commonly known technologies, including: dewatering, liming, thermoconditioning, methanogenesis or composting. One of the alternative solutions is the use of ozonation of raw excess sludge, which, in principle, may lead to the simultaneous resolution of several above-mentioned problems, such as: drainage properties improvement, increase the bioavailability of organic matter for biogas production, as well as the removal of undesirable microorganisms. Confirmation of the hygenizing effects usually requires arduous microbiological methods; therefore, the fast and reliable tools of molecular biology could prove their usefulness in this case. In this study, the possibility of determining the dynamics of selected groups of microorganisms (including changes in total bacteria amount, Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria) after sewage sludge ozonation, by using the semi-quantitative RealTime polymerase chain reaction (qPCR), wasinvestigated.
Źródło:: Journal of Ecological Engineering; 2019, 20, 3; 204-213
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Methods to Study the PVY Population in the Potato
Autorzy:: Yin, Zhimin
Powiązania:: https://bibliotekanauki.pl/articles/2199692.pdf
Data publikacji:: 2017-06-20
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: differential potato cultivars
multiplex RT-PCR
PVY strains classification
real-time RT-qPCR
sequencing
tobacco bait plants
Opis:: The PVY population in the potato has been studied continuously using tobacco bait plants in potato fields at Młochów since 1980 at two-year intervals and in potato tuber samples collected from different regions of Poland since 2001 yearly. The paper presents the combined biological, serological and molecular assays for PVY identification and strain classification. Biologically, PVY strains are defined with respect to their ability to elicit hypersensitive resistance (HR) mediated by N genes in differential potato cultivars (King Edward, Desiree and Pentland Ivory) and to symptoms in the tobacco (cultivar Samsun). Serologically, an ELISA assay based on polyclonal or monoclonal cocktail antibodies recognizes all PVY strain types, while the specif-ic monoclonal antibodies help to recognize PVYN or PVYO/PVYC strains. Multiplex RT-PCR, Real-time RT-qPCR and sequencing-based assays are used to define the PVY genome structure. In the Polish population of PVY, the strains PVYO, PVYNTN, PVYN-Wi, PVYZ-NTN and PVYE were identified, while the PVYC strain was not detected.
Źródło:: Plant Breeding and Seed Science; 2017, 75; 71-76
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
Autorzy:: Wolińska, L.
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Podlasz, P.
Kolwicz, S.
Piasecka, A.
Powiązania:: https://bibliotekanauki.pl/articles/363152.pdf
Data publikacji:: 2011
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: toksyczność ostra
CYP1A
cyp1b1
geny vtg
ekspresja mRNA
stężenie nie wywołujące efektu
WWA
Real-Time qPCR
acute toxicity
cyp1a genes
cyp1b1 genes
vtg genes
mRNA expression
No Effect Concentration
PAHs
Opis:: Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
Źródło:: Environmental Biotechnology; 2011, 7, 1; 26-33
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki