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    Wyświetlanie 1-8 z 8
    Tytuł:
    Genetic diversity of F1 and F2 interspecific hybrids between dwarf birch (Betula nana L.) and Himalayan birch (B. utilis var. jacquemontii (Spach) Winkl. 'Doorenbos') using RAPD-PCR markers and ploidy analysis
    Autorzy:
    Czernicka, Małgorzata
    Pławiak, Jarosław
    Muras, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1039274.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Betula nana
    Betula utilis 'Doorenbos'
    interspecific hybrids
    ploidy analysis
    RAPD-PCR
    Opis:
    Crosses between Betula nana and B. utilis 'Doorenbos' were undertaken in order to obtain interspecific hybrids which could be characterized by wide spreading stems, strong branching habit, decorative clear white bark and an interesting shape of purple leaves. The research purpose was to examine genetic diversity of the 16 F1 and F2 putative progenies by using the RAPD-PCR method and the ploidy analysis. A total of 242 RAPD markers were scored with 24 primers and 220 (90.9%) polymorphic bands were found. In the NJ dendrogram, cluster I consisted of the female parent - B. nana and 12 hybrids and cluster II grouped the male parent - B. utilis 'Doorenbos' with 4 hybrids (F2/2, F1/8, F1/7 and F2/1). The 2-D scaling by PCoA was in agreement with the similarity index, i.e. two hybrids (F1/8, F2/2) grouped with the male parent while others with female parent. Classification of the hybrid plants by chromosome counting demonstrated that 13 hybrids were confirmed with accurate chromosome counts as being diploid (2n=2x=28) and 3 plants (F1/7, F1/8, F2/2) as triploid with 42 chromosomes.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 195-199
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker
    Autorzy:
    Mullainathan, L.
    Sridevi, A.
    Umavathi, S.
    Sanjai Gandhi, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/11592.pdf
    Data publikacji:
    2014
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    genetic variation
    mutant
    chilli
    Capsicum annuum
    random amplified polymorphic DNA analysis
    RAPD marker
    spice
    Opis:
    The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
    Źródło:
    International Letters of Natural Sciences; 2014, 06
    2300-9675
    Pojawia się w:
    International Letters of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Higher induction of defense enzymes and cell wall reinforcement in maize by root associated bacteria for better protection against Aspergillus niger
    Autorzy:
    Jha, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2084643.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Aspergillus niger
    β-1
    3-glucanase
    biotic factor
    phenylalanine ammonia lyase
    RAPD analysis
    rhizosphere
    root associated bacteria
    Opis:
    Root associated bacteria were isolated from Suaeda nudiflora and two isolates were selected for this study: rhizospheric Bacillus megaterium and endophytic Pseudomonas aeruginosa. These isolates were inoculated into maize variety Narmada Moti during its germination. TTC (2, 3, 5-triphenyl tetrazolium chloride) staining was used to confirm the association of the isolates with the maize root. The effects of these root associated bacteria were tested alone and in combinations for cell wall reinforcement and the induction of defense enzymes such as phenylalanine ammonia lyase (PAL) and β-1,3-glucanase in the presence of fungal pathogen Aspergillus niger in maize. The results indicated that the rhizospheric bacteria had a greater fight response to fungal infection than the endophhytic bacteria due to cell wall lignification as well as the rapid induction of higher concentrations of defense related enzymes.
    Źródło:
    Journal of Plant Protection Research; 2019, 59, 3; 341-349
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers
    Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD
    Autorzy:
    Okoń, S.
    Paczos-Grzęda, E.
    Łoboda, M.
    Sugier, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542962.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    DNA polymorphism
    identification
    genetic diversity
    Arnica montana
    genotype
    RAPD marker
    medicinal plant
    molecular analysis
    Opis:
    Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.
    Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 63-71
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of new Polish lines of Chenopodium quinoa (Willd.) by spectral analysis of pigments and a confirmation of genetic stability with SCoT and RAPD markers
    Autorzy:
    Lema-Rumińska, J.
    Miler, N.
    Gęsiński, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/11887402.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant breeding
    quinoa
    Chenopodium quinoa
    Faro cultivar
    Titicaca cultivar
    morphological feature
    spectral analysis
    pigment
    genetic stability
    RAPD marker
    SCoT marker
    Opis:
    Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 75-86
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular characterization of Iranian black cumin (Nigella sativa L.) accessions using RAPD marker
    Autorzy:
    Neghab, M.G.
    Panahi, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/81098.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    black cumin
    Nigella sativa
    Iranian black cumin
    Ranunculaceae
    flowering plant
    RAPD marker
    genetic variation
    polymorphism
    UPGMA method
    clustering algorithm
    cluster analysis
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of bulk segregant analysis to identify a RAPD marker linked to the Mla locus of barley
    Autorzy:
    Czembor, Paweł Cz.
    Czembor, Jerzy H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2198932.pdf
    Data publikacji:
    2004-06-20
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    barley
    Blumeria graminis f. sp. hordei
    bulked segregant analysis
    DNA marker
    Mla locus
    RAPD
    Opis:
    Resistance to powdery mildew, Blumeria graminis f.sp. hordei, is a major goal of many barley breeding programs. Resistance conferred by genes located at Mla locus is commonly used by barley breeders for effective control of powdery mildew. The use of molecular markers may facilitate barley breeding for powdery mildew resistance. In this study, bulked segregant analysis (BSA) was used to determine random amplified polymorphic DNAs (RAPDs) markers linked to Mla locus. Thirty one homozygous (17 resistant and 14 susceptible) F3 families from a cross between variety Pallas and single plant line E 1059-1-1 carrying gene at Mla locus were used as plant material. A total of 385 random 10-mer primers were screened to identify polymorphism between the appropriate resistant and susceptible DNA bulks and parents in BSA analysis. Only one PCR marker OPAA3400 (primer sequence: 5’-TTAGCGCCCC-3’), amplified in polymerase chain reaction (PCR) proved close linkage and was positioned in distance of 10 cM from Mla locus with 5.0 LOD threshold.
    Źródło:
    Plant Breeding and Seed Science; 2004, 49; 41-49
    1429-3862
    2083-599X
    Pojawia się w:
    Plant Breeding and Seed Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zróżnicowanie genetyczne wybranych populacji sosny zwyczajnej (Pinus sylvestris L.) na podstawie analiz RAPD
    Genetic diversity of Scots pine (Pinus sylvestris L.) Polish provenances based on RAPD analysis
    Autorzy:
    Nowakowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/973034.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    drzewa leśne
    leśnictwo
    within− and among population genetic diversity
    Scots pine
    RAPD analysis
    metoda RAPD
    sosna zwyczajna
    Pinus sylvestris
    genetyka roślin
    populacje roślin
    zróżnicowanie genetyczne
    Opis:
    Genetic diversity is one of the key requirements for the adaptive potential of forest trees. This study presents the analysis of 30 Polish Scots pine provenances. According to the RAPD method, three 10−mer primers: OPE−08, OPE−09 and OPF−07 (Operon Technologies) were used to generate most polymorphic bands of amplified DNA among 450 individuals. The genetic similarity index was calculated after Nei (1987) matching coefficient and results were presented as dendrogram of genetic distances obtained from cluster analysis (UPGMA) for all populations. The Scots pine provenances were also characterized by height and diameter at the breast height „DBH” traits and were compared with the genetic diversity level. All studies populations were characterized by genetic diversity GST=0,215. This coefficient of inter−popu− lation variation was lower than the level of the differentiation within populations (HT=0,262), as it was reported for other Pine species. The results from these studies may be useful to the forest practice to help with decisions on the establishment of stable forest stands; they are also used in the forest genetic resources conservation programmes.
    Źródło:
    Sylwan; 2003, 147, 11; 26-37
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-8 z 8

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