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Wyszukujesz frazę "Phytophthora alni" wg kryterium: Temat

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    Tytuł:
    Charakterystyka morfologiczna i fizjologiczna izolatów Phytophthora alni otrzymanych z chorych olszy, gleby i wody
    Morphological and physiological characteristic of Phytophthora alni isolates obtained from diseased alder, soil and water
    Autorzy:
    Trzewik, A.
    Orlikowska, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/973841.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    grzyby
    izolaty grzybowe
    cechy fizjologiczne
    cechy morfologiczne
    fitopatologia
    mikologia
    Phytophthora alni
    czynniki chorobotwórcze
    alder decay
    phytophthora alni
    morphological and physiological characteristic
    Opis:
    The morphological and physiological features of 31 Phytophthora alni isolates from diseased alder, soil and water samples were determined. Optimum temperature for growth, sporangia produced and sex organs were examined.
    Źródło:
    Sylwan; 2011, 155, 01; 63-69
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molekularna diagnostyka wybranych patogenów z rodzaju Phytophthora w ramach integrowanej ochrony roślin
    Molecular diagnostic of Phytophthora pathogens as a tool for Integrated Pest Management
    Autorzy:
    Nowakowska, J.A.
    Malewski, T.
    Tereba, A.
    Borys, M.
    Oszako, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/989551.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    fitopatologia
    Phytophthora
    wykrywanie
    identyfikacja
    Phytophthora alni subsp.multiformis
    Phytophthora lacustris
    Phytophthora taxon hungarica
    metody badan
    metoda real time PCR
    sondy TaqMan
    real time pcr
    taqman
    butt and fine root pathogens
    phytophthora
    Opis:
    Traditional detection methods such as baiting or direct isolation take a long time and are incapable to handling large volume of material to be tested. The real−time PCR−based techniques are faster, more sensitive, more easily automated, and do not require post−amplification procedures. Species−specific primers for Phytophthora were designed based on the internal transcribed spacer regions (ITS) of rDNA collected from the NCBI DNA database. Primers and probes were designed using the Allele ID 7 at default search criteria. Specific probes were labeled with the reporter dyes JOE (6−carboxy−4,5−dichloro−2,7−dimethoxyfluorescein) at the 5' end and HBQ1 quencher at the 3' end (Sigma−Aldrich). The specificity of primers and fluorogenic probes was tested against genomic DNA of P. alni subsp. multiformis, P. lacustris and P. taxon hungarica. The real−time PCR reactions with the specific probes and primers yielded positive results with five concentrations of standards obtained by standard PCR reaction for corresponding Phytophthora species. The negative control (lack of DNA pathogens) yielded no amplification products. Standard curves showed a linear correlation between input DNA and cycle threshold (Ct) values with R² from 0.994 (P. alni) to 0.998 (P. taxon hungarica). The amplification efficiency of target DNA varied from 94.6% (P. alni) to 100% (P. taxon hungarica). The validation of the primers and probes designed for analysed Phytophthora species was performed on pure cultures, on soil samples from the forest nursery and declining oak stands. The designed probes displayed the high specificity of the detection of investigated species in pure cultures. The presented new molecular TaqMan probes can fully assist the integrated pest management as a powerful tool for a quick detection of above pathogenic organisms in forest nurseries. The molecular detection of harmful phytophthoras and in consequences diminishing of fungicides use for their control in forestry fully support European Union directives as well as the ‘Good plant protection practice measures' elaborated by European and Mediterranean Organisation of Plant Protection.
    Źródło:
    Sylwan; 2016, 160, 05; 365-370
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Patogenne lęgniowce z rodzaju Phytophthora - nowe zagrożenie lasów w Europie
    Pathogenic oomycetes of Phytophthora genus - a new threat to forests in Europe
    Autorzy:
    Ajchler, M.
    Łobocka, M.
    Oszako, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/986789.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    fitopatologia lesna
    zagrozenia drzewostanow
    czynniki chorobotworcze
    legniowce
    Oomycetes
    Phytophthora
    fytoftoroza
    identyfikacja
    epidemiologia
    Phytophthora alni
    Phytophthora cactorum
    Phytophthora cambivora
    Phytophthora cinnamomi
    Phytophthora citrophthora
    Phytophthora quercina
    Phytophthora plurivora
    Phytophthora ramorum
    ochrona lasu
    zwalczanie chorob roslin
    oomycetes
    phytophthora
    molecular typing
    plant pathogens
    biocontrol
    Opis:
    Pathogenic oomycetes represented mainly by the species of Phytophthora genus are among the most dangerous plant pathogens. They pose a serious threat for agricultural as well as wild plants, and are involved in forest decline worldwide. Over 140 pathogenic Phytophthora species have been identified so far. The common infection symptoms include rotting of below− and above− ground parts of plants, causing weakness and slow decline of infected trees. The economic losses caused by certain Phytophthora species may rich even 100%. Globalization and border opening have facilitated the transport of plant material between countries and continents, thus increased the risk of transfer of various Phytophthora genus representatives to new geographical locations. Global warming (e.g. mild winters) have facilitated the expansion of species from southern to northern Europe. Among Polish Phytophthora isolates are species that have previously been known only in nurseries (e.g. P. cactorum), but nowadays they are also isolated from forests (e.g. oak stand in the Krotoszyn Plateau). It suggests the pathway from nurseries to stands with plant for plantings and attached soil. There are also new species, that have not been isolated so far in the world (P. polonica) or found far away from Poland (P. fragaliaefolia in Japan on strawberry). The possible natural pathways are birds and water courses. In Mazowsze and Wielkopolska regions (C and W Poland) the polyphagous P. cinnamomi was found on pedunculate oaks (Quercus robur). This species causes significant damage to red oak forest in France, but also threats Jarrah forests in the Australian ecosystem (it attacks ca. 1000 species of plants). Fortunately, in addition to time consuming and laborious classic methods of Phytophthora identification, based on morphology and physiological properties, molecular methods that are based on immunological tests and chromosomal or mito− chondrial DNA markers identification have come into common use. Despite morphological similarity to true fungi, oomycetes are more closely related to diatoms and brown algae, and have several structural features that differentiate them from fungi, including the cell wall composed of cellulose instead of chitin. That is one of the reasons that fungicides have a limited use in the fight with Phytophthora infections. Additionally, type of ecological niches that are settled by pathogenic oomycetes (root remnants in soil, watercourses) hinders the chemical combating. Biocontrol, i.e. the use of interspecies interactions between microorganisms (bacteria, fungi) to limit the growth and development of pathogens, seems to be a reasonable alternative.
    Źródło:
    Sylwan; 2017, 161, 10; 870-880
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Przydatność pułapek roślinnych i pożywki ziemniaczano− −glukozowej do izolacji Phytophthora alni z porażonych tkanek olszy oraz z gleby
    The usefulness of traps and PDA medium for Phytophthora alni isolation from infected tissues and soil
    Autorzy:
    Oszako, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/972870.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    pozywka PDA
    grzyby
    Phytophthora alni
    jablka
    rosliny pulapkowe
    lesnictwo
    izolacja
    fitopatologia lesna
    biological traps: apple
    rhododendron leaves
    Opis:
    The purpose of the study was to determine the usefulness of green apples as well as leaves of rhododendron as trapping methods for isolation of Phytophthora alni in disease tissues and in the soil. Apple traps as well as rhododendron leaves may be used successfully for isolation of the pathogen from roots, stem collar and from the soil, respectively. Such baits are particularly recommended for pathogen isolation from mature trees what is usually more difficult than in the case of seedlings. Apart of P. alni, faster growing of Fusarium, Botrytis, Cladosporium, Chaetomium and Trichoderma species on PDA medium were also isolated from the base of stems of diseased alders. It could be also the reason why P. alni was not always isolated from diseased tissues.
    Źródło:
    Sylwan; 2006, 150, 04; 59-64
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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