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Wyszukujesz frazę "Metagenome" wg kryterium: Temat


Wyświetlanie 1-4 z 4
Tytuł:
Identification of antimicrobial resistance genes in intestinal content from Coyote (Canis latrans)
Autorzy:
López-Islas, J.J.
Méndez-Olvera, ET.
Reyes C, T.
Martínez-Gómez, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647515.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
antimicrobial resistance
metagenome
Canis latrans
Opis:
Antibiotic resistance has become a global public health concern in the last few years. Given the widespread rate of recurrence, increasing attention is being turned toward environmental pathways that potentially contribute to antibiotic resistance genes (ARGs) dissemination outside the clinical realm. In this study, a metagenome analysis of intestinal virus-like particle fraction (VLPs) from a wild coyote (Canis latrans) revealed for the first time, multiple ARGs, such as B-lactamases and multidrug efflux pumps. Description of ARGs presence in natural environments is critical to understand the emergence of resistant strains.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 143-149
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular diversity of the bacterial community associated with Acropora digitifera (Dana, 1846) corals on Rancabuaya coastline, Garut District, Indonesia
Autorzy:
Rizal, Achmad
Akbarsyah, Nora
Kdyp, Pringgo
Permana, Rega
Andhikawati, Aulia
Powiązania:
https://bibliotekanauki.pl/articles/1030642.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Acropora digitifera
Bacteria
Culture
Gen 16S rRNA
Metagenome
Opis:
Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in the rancabuaya coastline of Garut district, West Java through a metagenomic and cultural approach. Stages of research include tissue isolation using waterpics, isolation of bacterial genomic DNA, sequencing using the NGS (Next Generation Sequencing) method of Hiseq using 16S rRNA V3-V4 region 341F and 806R primers for the metagenomic approach. While the culture approach, carried out inoculation, bacterial cultivation, gram staining, then proceed with the identification of molecular characteristics of DNA with 16S rRNA gene sequences. Dominantly, the results of bacterial identification were obtained as many as 77 species from 10 genera 10 families 10 orders of 10 classes and 4 phylum and unidentified reads of 6%. The results of the identification of 16S rRNA showed that the isolate ACD.P4.PH7.P had a close relationship with the Bacillus flexus strain BF strain zb strain with a similarity of 85.44%. Isolate ACD.P4.PH9.P has a close relationship with Bacillus sp. c234 with a 98.50% similarity. Isolate ACD.P4.PH9.K has an approach with the species Bacillus sp. strain 6RM1 with a similarity of 94.78%.
Źródło:
World Scientific News; 2020, 144; 384-396
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in Essential Soil Nutrients and Soil Disturbance Directly Affected Soil Microbial Community Structure: A Metagenomic Approach
Autorzy:
Wiryawan, Adam
Eginarta, Wahyu Satria
Hermanto, Feri Eko
Ustiatik, Reni
Dinira, Layta
Mustafa, Irfan
Powiązania:
https://bibliotekanauki.pl/articles/2173263.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
forest soil
metagenome
soil bacteria
soil nutrients
tilled land
Opis:
Soil environment, both biotic (e.g., microbial community) and abiotic (e.g., nutrients and water availability) factors determine soil fertility and health and are directly affected by soil management systems. However, only limited studies evaluate the combined effect of nutrients availability and soil disturbance on the soil bacteria community structure, especially in conventional agricultural practices, on the forests converted to agricultural land. This study aimed to provide a viewpoint of the effect of different soil management systems, i.e., forest soil (natural process) and tilled land, on soil bacteria community structure on forest converted to agricultural land, according to a metagenomics approach. Moreover, each land use was sampled to identify the bacterial community using 16S gene as a biomarker. The sequencing was performed using MinION (Oxford Nanopore Technologies) to read the DNA sequence from each soil sample. Principle Component Analysis (PCA) was performed to comprehend the relationship between availability of nutrients and bacterial diversity. The results revealed that the concentrations of soil micronutrients, such as iron, zinc, and magnesium, were significantly higher in forest soil than in tilled land. According to diversity indices, soil bacteria were more diverse in forest soil than in tilled land. Forest soil had more distinctive taxa than tilled land. Several species comprised the most abundant taxa, such as Candidatus Koribacter versatilis, Candidatus Solibacter usiatus, Rhodoplanes sp., Luteitalea pratensis, and Betaproteobacteria bacterium, were more scarce in tilled land. On the distinctive taxa in each soil sample, Anseongella ginsenosidimutans and Janthinobacterium sp. were the most abundant species in forest and tilled land, respectively. According to PCA analysis, soil management system affected the soil micro-and macronutrients also microbial community structure between forest and tilled land. In conclusion, soil management influences the essential nutrient content and bacterial community structure of soil. Better management should be adopted to maintain soil quality near forest soil.
Źródło:
Journal of Ecological Engineering; 2022, 23, 7; 238--245
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Progress of medically-related biotechnology in the post-genomic era
Autorzy:
Kozlowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/80941.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Arabidopsis
biotechnology
copy number variation
enormous progress
eukaryote
genetic marker
genome
medical biotechnology
metagenome
microarray technology
nematode
non-coding RNA
stem cell
yeast
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-4 z 4

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