- Tytuł:
- Influence of Me2SO and incubation time on papain activity studied using fluorogenic substrates.
- Autorzy:
-
Szabelski, Mariusz
Stachowiak, Krystyna
Wiczk, Wiesław - Powiązania:
- https://bibliotekanauki.pl/articles/1044042.pdf
- Data publikacji:
- 2001
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
fluorogenic substrate
enzyme activity
papain
Me2SO - Opis:
- Papain activity in a buffer containing Me2SO was studied using fluorogenic substrates. It was found that the number of active sites of papain decreases with increasing Me2SO concentration whereas the incubation time, in a buffer containing 3% Me2SO does not affect the number of active sites. However, an increase of papain incubation time in the buffer with 3% Me2SO decreased the initial rate of hydrolysis of Z-Phe-Arg-Amc as well as Dabcyl-Lys-Phe-Gly-Gly-Ala-Ala-Edans. Moreover, an increase of Me2SO concentration in working buffer decreased the initial rate of papain-catalysed hydrolysis of both substrates. A rapid decrease of the initial rate (by up to 30%) was observed between 1 and 2% Me2SO. Application of the Michaelis-Menten equation revealed that at the higher Me2SO concentrations the apparent values of kcat/Km decreased as a result of Km increase and kcat decrease. However, Me2SO changed the substrate binding process more effectively (Km) than the rate of catalysis kcat..
- Źródło:
-
Acta Biochimica Polonica; 2001, 48, 4; 995-1002
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł