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    Wyświetlanie 1-4 z 4
    Tytuł:
    Helicobacter pylori cytotoxin-associated gene A impairs the filtration barrier function of podocytes via p38 MAPK signaling pathway
    Autorzy:
    Yang, Man
    Wang, Ling
    Gu, Li-jie
    Yuan, Wei-jie
    Powiązania:
    https://bibliotekanauki.pl/articles/1038590.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CagA
    ZO-1
    p38 MAPK
    podocyte
    proteinuria
    Opis:
    Helicobacter pylori (Hp) specific antigens were found deposited in the glomeruli in some kidney diseases. However, the underlying molecular mechanisms remain to be elucidated. The aim of this study was to investigate the effect of cytotoxin associated gene A protein (CagA), a key virulence factor of Hp, on mouse podocytes. Cells were cultured and treated with recombinant CagA protein. The expression of the tight junction protein ZO-1 and p38 MAPK signaling pathway activation were measured with real-time RT-PCR and western blotting. The filtration barrier function of podocytes was evaluated with albumin influx assay. CagA decreased the expression and membrane distribution of ZO-1, impaired the filtration barrier function of podocytes, while activating p38 MAPK signaling pathway in these cells. Selective p38 MAPK inhibition partly prevented CagA-induced filtration barrier dysfunction of podocytes through ameliorating ZO-1 downregulation. Taken together, the results suggested that CagA, at least via p38 MAPK signaling pathway, may induce podocyte injury. Anti-Hp therapy may be beneficial for the treatment of kidney diseases related to Hp antigen deposition.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 3; 471-475
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of hemolysis and eryptosis by occupational pollutant nickel chloride is mediated through calcium influx and p38 MAP kinase signaling
    Autorzy:
    Alfhili, Mohammad A.
    Alamri, Hassan S.
    Alsughayyir, Jawaher
    Basudan, Ahmed M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085691.pdf
    Data publikacji:
    2022-02-15
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    calcium
    p38 MAPK
    oxidative stress
    hemolysis
    nickel
    eryptosis
    Opis:
    ObjectivesNickel (Ni) is an abundant environmental hazard and an occupational pollutant. Exposure to Ni compounds is prevalent in electroplating workers and in the printing industry, among others. The toxicity of Ni manifests as dermatological, gastrointestinal, respiratory, allergic, and cardiovascular symptoms. In particular, hyperbilirubinemia and reticulocytosis have been detected in intoxicated subjects; an observation possibly implicating selective red blood cell (RBC) toxicity. Herein, the interaction of nickel chloride (NiCl2) with human RBCs and associated molecular mechanisms are described.Material and MethodsCells from healthy donors were incubated for 24 h at 37°C in the presence or absence of 0.5‒10 mM of NiCl2, and cytotoxicity was determined through hemoglobin leakage by colorimetry under different experimental conditions. Eryptotic markers were also identified by flow cytofluorometry using Annexin-V-FITC tagging for phosphatidylserine (PS) exposure, light scatter properties for cellular dimensions, Fluo4/AM labeling for intracellular calcium, and H2DCFDA staining for reactive oxygen species (ROS). Additionally, small molecule inhibitors were used to probe the signaling pathways involved.ResultsIt was found that NiCl2 at 10 mM caused profound intracellular calcium overload and significant calcium-dependent hemolysis. Also, NiCl2 reduced forward scatter and increased side scatter, Annexin-positive cells, and ROS levels. Importantly, NiCl2-induced hemolysis was significantly attenuated by the exclusion of extracellular calcium, and in the presence of p38 MAP kinase (MAPK) inhibitor SB203580.ConclusionsIt is concluded that NiCl2 induces p38 MAPK-dependent hemolysis, and stimulates the canonical features of premature eryptosis. This report presents the first description of the molecular mechanisms underlying the hemolytic and eryptotic potential of NiCl2 and, thus, may explain changes in hematological parameters observed in poisoning victims.
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2022, 35, 1; 1-11
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of Epilobium angustifolium extract on 5alpha-reductase type 2 and MAPK3 kinase gene expression in rats prostates
    Wpływ wyciągu z Epilobium angustifolium na ekspresję genów 5alfa-reduktazy typu 2 oraz kinazy MAPK3 w szczurzych prostatach
    Autorzy:
    Kujawski, R.
    Bartkowiak-Wieczorek, J.
    Karasiewicz, M.
    Bogacz, A.
    Mikolajczak, P.L.
    Czerny, B.
    Mrozikiewicz, P.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/72333.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    Epilobium angustifolium
    plant extract
    5alpha-reductase type 2
    Mapk3 gene
    kinase
    gene expression
    rat
    prostate
    androgen receptor
    Opis:
    The aim of this study was to investigate the influence of standardized Epilobium angustifolium L. extract [100 mg/kg/day, p.o.] on the expression level of 5α-reductase type 2 (Srd5ar2) mRNA and Mapk3 mRNA a representative of non-genomic xenobiotics signaling pathway. It was shown that plant extract from the E. angustifolium showed a slight tendency to reduce prostate weight in hormonally induced animals (p>0.05) and in testosterone induced animals receiving both, extract and finasteride (p<0.05). Finasteride in rats induced by testosterone caused a smaller decrease in the level of mRNA 5α-steroid reductase 2 (SRd5ar2), than in rats treated with the hormone and studied plant extracts. In general, an increase in the amount of MAPK3 mRNAs in testosterone-induced groups of rats receiving tested plant extract with or without finasteride was observed, while the expression of type 2 5α-steroid reductase decreased (p<0.05). Further experimental studies should be performed in order to understand the molecular basis of interactions, the efficacy and safety of tested plant extracts.
    Celem pracy było zbadanie wpływu standaryzowanego ekstraktu z ziela Epilobium angustifolium L. [100 mg/kg/dzień, p.o.] na poziom ekspresji mRNA 5α-reduktazy typu 2 (SRd5ar2) oraz mRNA kinazy MAPK3 – przedstawiciela androgenozależnego, nie-genomowego szlaku sygnalizacji komórkowej. W zastosowanym modelu eksperymentalnym wyciąg z z E. angustifolium wykazał statystycznie nieistotną, niewielką tendencję do zmniejszania masy prostat u zwierząt indukowanych hormonalnie (p>0,05) oraz u szczurów indukowanych testosteronem, otrzymujących zarówno ekstrakt, jak i finasteryd (p<0,05). Finasteryd u szczurów otrzymujących testosteron spowodował mniejsze, aniżeli zakładano, obniżenie poziomu mRNA 5α-reduktazy typu 2 (SRd5ar2), niż u szczurów, którym podano hormon i badany wyciąg (p <0.05). Stwierdziliśmy ponadto, zwiększenie ilości mRNA kinazy MAPK3 u szczurów indukowanych testosteronem otrzymujących badany ekstrakt, wraz z finasterydem lub bez niego, podczas gdy ekspresja reduktazy w tych grupach uległa zwiększeniu (p <0,05). Należy przeprowadzić dalsze badania eksperymentalne w celu zrozumienia molekularnych podstaw oddziaływań, skuteczności i bezpieczeństwa badanych ekstraktów roślinnych.
    Źródło:
    Herba Polonica; 2013, 59, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Crosstalk between the TGF-β and WNT signalling pathways during cardiac fibrogenesis
    Autorzy:
    Działo, Edyta
    Tkacz, Karolina
    Błyszczuk, Przemysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1038355.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    TGF-beta
    Smad
    WNT
    beta-catenin
    RhoA-ROCK
    p38
    JNK
    Erk1/2
    MAPK
    cardiac fibrosis
    tissue remodelling
    cardiac fibroblasts
    heart
    Opis:
    Cardiac fibrosis is referred to as an excessive accumulation of stromal cells and extracellular matrix proteins in the myocardium. Progressive fibrosis causes stiffening of the cardiac tissue and affects conduction of electrical impulses, leading to heart failures in a broad range of cardiac conditions. At the cellular level, activation of the cardiac stromal cells and myofibroblast formation are considered as hallmarks of fibrogenesis. At the molecular level, transforming growth factor β (TGF-β) is traditionally considered as a master regulator of the profibrotic processes. More recently, the WNT signalling pathway has also been found to be implicated in the development of myocardial fibrosis. In this review, we summarize current knowledge on the involvement of TGF-β and WNT downstream molecular pathways to cardiac fibrogenesis and describe a crosstalk between these two profibrotic pathways. TGF-β and WNT ligands bind to different receptors and trigger various outputs. However, a growing body of evidence points to cross-regulation between these two pathways. It has been recognized that in cardiac pathologies TGF-β activates WNT/β-catenin signalling, which in turn stabilizes the TGF-β/Smad response. Furthermore both, the non-canonical TGF-β and non-canonical WNT signalling pathways, activate the same mitogen-activated protein kinases (MAPKs): the extracellular signal-regulated kinase (Erk), the c-Jun N-terminal kinases (JNKs) and p38. The crosstalk between TGF-β and WNT pathways seems to play an essential role in switching on the genetic machinery initiating profibrotic changes in the heart. Better understanding of these mechanisms will open new opportunities for development of targeted therapeutic approaches against cardiac fibrosis in the future.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 3; 341-349
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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