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Wyświetlanie 1-3 z 3
Tytuł:
Upper respiratory tract infections and influenza-like illnesses among healthcare workers: are serological tests useful in monitoring influenza and influenza-like illness?
Autorzy:
Toczek-Kubicka, Katarzyna
Szenborn, Filip
Kuchar, Ernest P.
Szenborn, Leszek
Powiązania:
https://bibliotekanauki.pl/articles/2153866.pdf
Data publikacji:
2022-12-29
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
IgM antibodies
vaccination
humoral response
influenza
healthcare workers
influenza-like illness
Opis:
Background Healthcare workers (HCWs) are at high risk for exposure to upper respiratory tract infections (URTIs) and influenza-like illnesses (ILIs). The present study aimed to surveil URTIs and ILIs and their impact among the Department of Pediatric Infectious Diseases in Wroclaw employees and evaluate their humoral response to influenza. Material and Methods Thirty-six HCWs participated in the first season and 32 HCWs in the second season during years of the study. The authors carried out a URTI/ILI surveillance, and all HCWs were asked to complete a weekly report during 2 influenza seasons: 2016/2017 (S1) and 2017/2018 (S2). In S1 both IgG and IgM antibodies against influenza A and B were assessed. The HCWs with symptoms of ILI were encouraged to undergo PCR tests for influenza. Results No significant differences in reporting URTI were found among vaccinated and non-vaccinated HCWs and HCWs and the control group. Depending on the year 5.5–17.2% of HCWs were treated with antibiotics because of URTI. In the study 58.7% of participants in S1 and 66.7% in S2 decide to work despite the URTI symptoms. There was no statistical relationship between the concentration of anti-influenza IgG and the number of URTIs and ILIs reported. Only vaccinated were willing to undergo voluntary influenza testing. Conclusions The URTI and ILI occur commonly in HCWs, and HCWs contract URTIs as often as the control group. Despite their medical education, HCWs work with the symptoms of infection and overuse antibiotics to treat the URTI. Serology testing is not able to follow the infection’s dynamics or identify the people immune to the influenza-like illness. The diagnostic value of IgM antibodies in acute influenza infection is negligible. Vaccinated HCWs are more focused on their health and are more willing to undergo influenza tests.
Źródło:
Medycyna Pracy; 2022, 73, 6; 441-447
0465-5893
2353-1339
Pojawia się w:
Medycyna Pracy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the efficiency of two commercial kits - ELFA and Western blot in estimating the phase of Toxoplasma gondii infection in pregnant women
Autorzy:
Sroka, Jacek
Wójcik-Fatla, Angelina
Zając, Violetta
Sawczyn, Anna
Cisak, Ewa
Karamon, Jacek
Dutkiewicz, Jacek
Bojar, Iwona
Powiązania:
https://bibliotekanauki.pl/articles/991156.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
toxoplasmosis
pregnant women
seroprevalence
antibodies
igg
igm
elfa
western blot
avidity
correlation of serologic tests
Opis:
Sera of 89 pregnant women were selected according to the results of ELFA IgM, IgG and avidity IgG, and tested with commercial tests IgM, IgG and avidity IgG Western Blot (WB) to compare the efficacy of both techniques in determining the phase of T. gondii infection. In total, 81 of 89 tested sera (91.0%) were classified as positive, both in the ELFA and WB tests for the presence of anti-Toxoplasma antibodies of class IgG, indicating a past infection, while the prevalence of anti-Toxoplasma positive reactions associated with the antibodies of class IgM indicating a recent infection was much lower – 31.5% and 20.2%, respectively. Sera of 81 women were also tested in the ELFA and WB tests for avidity, e.g. ability of forming high-molecular IgG antibody complexes. Low or medium results in these tests (in this study all classified as low), indicating a recent infection, were detected by ELFA and WB in 22.2% and 45.7% of the total examined samples, respectively. The Spearman’s rank test for correlation, performed for recognition of quantitative data of the ELFA and WB tests (index, units or points), revealed a highly significant correlation between the ELFA and WB tests for homologous classes of antibodies, both for IgM and IgG (p<0.00001). In contrast, the ELFA and WB tests for detection of anti-Toxoplasma IgM antibodies were not correlated with the ELFA and WB tests for detection of anti-Toxoplasma IgG antibodies (p>0.05), except for the WB test for IgM antibodies, which showed a significant correlation with the ELFA test for IgG antibodies (p<0.01). A highly significant negative correlation between the ELFA and WB test for IgM antibodies and ELFA and WB tests for IgG avidity was demonstrated (p<0.01), except for a relationship between the WB test for IgM and WB for avidity, which was not significant. Such negative correlations are theoretically expected, as strong complexes with the participation of IgG antibodies are absent in the early phase of toxoplasmosis when early antibodies of IgM class are present. Summarizing, this study indicates the high usefulness of the commercial ELFA and WB tests in serodiagnostics of toxoplasmosis in pregnant women. Special attention should be paid to parallel detection of IgM antibodies and low values in the ELFA and WB tests for IgG avidity, which indicates a recent infection which may be associated with a clinical form of congenital toxoplasmosis and damage to the foetus.
Źródło:
Annals of Agricultural and Environmental Medicine; 2016, 23, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibody - Biological warfare agents
Autorzy:
Mayrolle, Pierre
Kuillon, Jean Michel
Kuéry, Sareleï
Aulin, Vincent
Kirondot, Marc
Powiązania:
https://bibliotekanauki.pl/articles/1119235.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Immunoglobulin A (IgA)
Immunoglobulin G (IgG)
Polyclonal antibodies (PAb)
antibody production
antibody (Ab)
immunoglobulin M (IgM)
Opis:
An antibody (Ab), also known as immunoglobulin (Ig), is a large Y-shape protein consisting of two heavy chains and two light chains (Figure 1) which form a functionally bivalent monomer that is produced by B cells receptor used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. Current widely used rapid detection systems exploit antibodies for recognition, identification and quantification of target analytes [68]. Abs played an important role in the advancement of diagnostic assays, making these indispensable in diagnostic tests that are commonly used routinely in clinics in classical immunological methods such as ELISA, dot blot immunobinding assays, electro-chemiluminescence, flow cytometry and several microscopic techniques like fluorescence, as well as in the construction of numerous immunosensors. Immunoglobulin M (IgM) usually exists as a pentamer in mammals that predominates in primary immune responses to most antigens, and is the most efficient complement fixing immunoglobulin. It comprises approximately 10% of all normal human serum Ig content. IgM is mainly produced by the immune system for protection against numerous viral, bacterial, fungal and parasitic infections.
Źródło:
World News of Natural Sciences; 2016, 5; 1-19
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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