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    Wyświetlanie 1-7 z 7
    Tytuł:
    Novel Synthesis, spectral, characterization of 4,5-diphenyl-1-((tetrahydrofuran-2-yl)methyl)-2-(3,4,5-trichlorophenyl)-1H-imidazole and its applications of molecular docking, anticancer activity
    Autorzy:
    Dhineshkumar, E.
    doss, M. Arockia
    Uma, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/1031882.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    HepG2
    Imidazole
    cytotoxicity
    molecular docking
    Opis:
    In the present study of 4,5-diphenyl-1-((tetrahydrofuran-2-yl)methyl)-2-(3,4,5-trichlorophenyl)-1H-imidazole 1 was synthesized. The synthesized imidazole compound 1 has been characterized by FT-IR, 1H, 13C NMR and ESI-Mass spectral studies. Molecular docking is also performed in order to explain the over-expression of estrogen receptor in 70% of liver cancer. The imidazole scaffold is a privileged scaffold for exploration of anticancer agents. The objective of the present study is to evaluate the anticancer activity of imidazole 1 in human liver cancer cell lines HepG2.
    Źródło:
    World News of Natural Sciences; 2020, 30, 2; 203-212
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Japanese quince (Chaenomeles japonica L.) fruit polyphenolic extract modulates carbohydrate metabolism in HepG2 cells via AMP-activated protein kinase
    Autorzy:
    Zakłos-Szyda, Małgorzata
    Pawlik, Nina
    Powiązania:
    https://bibliotekanauki.pl/articles/1038525.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    AMPK
    diabetes
    glucose metabolism
    HepG2
    Japanese quince
    polyphenols
    Opis:
    Type 2 diabetes mellitus (T2D) is a chronic diet-related disease which due to many dangerous complications has become a prominent health problem of the world. The aim of the study was to explore the in vitro activity of Japanese quince (Chaenomeles japonica L., family Rosaceae, JQ) fruit polyphenolic extract as modulator of carbohydrates metabolism. The research was designed to investigate the effect of JQ polyphenolic extract on glucose metabolism in human hepatoma HepG2 cell line cultured under normal non-metabolically changed and hyperglycemic conditions. Pretreatment of the cells with JQ preparation caused decrease of intracellular ROS generation and influenced mitochondrial membrane polarization which seemed to lead to AMPK activation. Further effects observed in HepG2 cells were associated with activation of the enzyme: elevation of glucose uptake and glycogen content, and alleviation of gluconeogenesis through modulation of PEPCK, PTP1B, FOXO1 and GLUT2/4 expression. These findings suggest that JQ polyphenols exhibit hypoglycemic effects via modulation of AMPK signaling in hepatocytes.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 1; 67-78
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study
    Autorzy:
    Kheyar-Kraouche, Naoual
    Boucheffa, Saliha
    Bellik, Yuva
    Farida, Kheyar
    Brahmi-Chendouh, Nabila
    Powiązania:
    https://bibliotekanauki.pl/articles/16706188.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Inula viscosa leaf extracts
    antioxidant
    cytotoxic effect
    HepG2 cells
    ROS
    molecular docking
    Opis:
    In folk medicine, Inula viscosa (Asteraceae) has been traditionally utilized for treating various ailments, including diabetes, bronchitis, diarrhea, rheumatism, and injuries. In this study, we aimed to investigate the chemical composition, antioxidant, antiproliferative, and apoptotic properties of I. viscosa leaf extracts. Extraction was performed using solvents of varying polarities. Antioxidant activity was determined using Ferric reducing antioxidant power (FRAP) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The results revealed that aqueous ethanol (70%) and aqueous ethyl acetate (70%) extracts contained high levels of phenols (645.58 ± 8.77 mg CE/g) and flavonoids (180.69 ± 1.54 mg QE/g), respectively. Aqueous ethanol (70%) extract exhibited the highest antioxidant activity with IC50 of 572.74 μmol TE/g DW (μmol Trolox equivalent in 1g of dry extract) in the ABTS assay and 76 862.06 μM TE/g DW in the FRAP test. All extracts showed a considerable dose-dependent cytotoxic effect on cancerous HepG2 cells (P < 0.05). The aqueous ethanol extract demonstrated the highest inhibitory effect (IC50 = 1.67 mg/ml). Treatment with aqueous ethanol (70%) and pure ethyl acetate extracts significantly increased the number of apoptotic cells to 8 and 6%, respectively, in HepG2 cells (P < 0.05). Additionally, the aqueous ethanol extract significantly elevatedreactive oxygen species (ROS) levels (53%) in HepG2 cells. The molecular docking study identified paxanthone and banaxanthone E as the compounds that exhibited the highest binding affinities with BCL-2. This study demonstrated the potent antioxidant, antiproliferation, and intracellular ROS production of I. viscosa leaf extracts. Further studies should be conducted to identify the active compounds involved.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 2; 183-198
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-γ1 and -γ2 mRNAs
    Autorzy:
    Rypka, Miroslav
    Veselý, Jaroslav
    Powiązania:
    https://bibliotekanauki.pl/articles/1040405.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    peroxisome proliferator-activated receptor
    HepG2 cells
    mRNA stability
    PPAR-γ coactivator
    superinduction
    Opis:
    Quantitative real-time RT-PCR study was conducted to reveal the effects of normal (5 mmol/l) and high (30 mmol/l) glucose without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)α, -γ1, -γ2, and peroxisome proliferator-activated receptor-γ coactivator- (PGC-)1α and -1β in commercial human hepatoma-derived HepG2 cells maintained under low-serum condition. Significant decrease in PPAR-γ1 and PGC-1α mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, both PPAR-γ1 and PGC-1α mRNAs were partly but significantly restored in high glucose batches. In this period, the presence of the transcriptional inhibitor actinomycin D revealed a significant protective effect of excess glucose on mature PPAR-γ1 and PGC-1α mRNAs. Furthermore, PPAR-γ1 and -γ2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide. When the cells were co-treated with the combination of cycloheximide and actinomycin D, superinduction was completely suppressed, however. Altogether, the experiments revealed, first, an unexpected protective effect of abundant glucose on PPAR-γ1 and PGC-1α mRNAs in HepG2 cells. Second, we demonstrated cycloheximide-induced, transcription-dependent upregulation of mature PPAR-γ1 and -γ2 mRNAs in HepG2 cells associated with preferential expression of the PPAR-γ2 mRNA variant. The results draw attention to as yet unexplored mechanisms involved in the control of PPAR and PGC genes.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 2; 209-215
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Induction of ROS by a novel chromone linked nitrone derivative promotes mitochondria mediated caspase dependent apoptosis in HepG2 and HeLa cells
    Autorzy:
    Mandal, Supratim
    Mallick, Suvadip
    Maiti, Sourav
    Bandyopadhya, Chandrakanta
    Pal, Chiranjib
    Powiązania:
    https://bibliotekanauki.pl/articles/1177437.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Anticancer activity
    Apoptosis
    Caspases
    Chromones
    HeLa-HepG2 cell lines
    Mitochondrial membrane potential
    Reactive oxygen species generation
    Opis:
    Chromones are organic compounds reported to induce cytotoxic effect in an extensive variety of cells. Consequently, the synthesis and reorientation of the chromone molecules are of great interest for many researchers because of their miscellaneous biological activities. The present study was designed to assess the significant antitumor effects of C-(6-Methyl-4-oxo-4H-1-benzopyran-3-yl)-N-(p-tolyl) nitrone, a novel chromone linked nitrone derivative and to elucidate the mechanism of these effects on two human cancer cell lines HepG2 and HeLa. Cell proliferation was analysed by the MTT assay. Apoptosis was evaluated by DAPI staining and flow cytometric analysis and quantified by fluorometric assays for Caspase 3 and 9. Apaf-1 and cytochrome c expression were identified by means of Western Blot analysis. The derivative showed significant dose dependent cytotoxic effects in the cancer cells and induced the reactive oxygen species and endogenous nitric oxide production. Furthermore, mitochondrial membrane potential depolarization, translocation of mitochondrial cytochrome c to cytosol, induction of Apaf-1 and activation of caspases were observed during the derivative-mediated apoptosis. These findings proposed that the novel chromone linked nitrone derivative has significant antitumor effects on HepG2 and HeLa cells and have immense scope to develop as an anticancer agent.
    Źródło:
    World Scientific News; 2018, 103; 167-185
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification of lipid derivatives in Hep G2 cells
    Autorzy:
    Gdula-Argasińska, Joanna
    Garbacik, Aneta
    Tyszka-Czochara, Małgorzata
    Woźniakiewicz, Michał
    Paśko, Paweł
    Czepiel, Jacek
    Powiązania:
    https://bibliotekanauki.pl/articles/1039495.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    human hepatocellular carcinoma cells HepG2
    eicosapentaenoic acid
    benzo(a)pyrene
    isoprostanes
    prostaglandins
    UHPLC/MS-TOF method validation
    Opis:
    Metabolism of polyunsaturated fatty acids results in biosynthesis of mediators with different physiological effects. These metabolites include prostaglandins, prostacyclins, isoprostanes and others that are important signalling molecules and regulate a variety of physiological and pathophysiological processes including inflammation. Prostaglandins and isoprostanes are produced by either non-enzymatic lipid peroxidation or by enzyme-induced peroxidation (cyclooxygenases and lipoxygenases). They are used as biomarkers of oxidative stress. The aim of our study was to assess the effect of eicosapentaenoic acid (EPA) supplementation with added benzo(a)pyrene (BaP) on HepG2 cells by using a UHPLC/MS-TOF method. This rapid and simple method was developed for the identification, separation and quantification of 8-iPGF3α, PGF3α, 8-isoPGF2α and 5-iPF2α in cultured cells. The UHPLC/MS-TOF method was validated. The calculated limit of detection was in the range of 0.16-0.50 ng/mL, precision (% RSD): 1.2-2.1% and recoveries better than 88%. This method empowered qualitative and quantitative analysis of the selected individual prostaglandins derived from arachidonic acid and eicosapentaenoic acid from cell extracts.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 811-815
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In silico and in vitro cytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2)
    Autorzy:
    El-Batal, A.I.
    El-Hendawy, H.H.
    Faraag, A.H.I.
    Powiązania:
    https://bibliotekanauki.pl/articles/80210.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    silver nanoparticle
    liver cancer
    cancer cell line
    HepG2 cell
    Serratia marcescens
    cytotoxic activity
    in silico analysis
    mitogen-activated protein kinase
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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