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Tytuł:
Analysis of uropathogenic Escherichia coli biofilm formation under different growth conditions
Autorzy:
Adamus-Białek, Wioletta
Kubiak, Anna
Czerwonka, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038911.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biofilm
uropathogenic Escherichia coli
urinary tract infections
Opis:
The ability to form different types of biofilm enables bacteria to survive in a harsh or toxic environment. Different structures of biofilms are related to different surfaces and environment of bacterial growth. The aim of this study was analysis of the biofilm formation of 115 clinical uropathogenic Escherichia coli strains under different growth conditions: surface for biofilm formation, medium composition and time of incubation. The biofilm formation after 24 h, 48 h, 72 h and 96 h was determined spectrophotometrically (A531) after crystal violet staining and it was correlated with bacterial growth (A600). The live and dead cells in biofilm structures was also observed on the glass surface by an epi-fluorescence microscope. Additionally, the presence of rpoS, sdiA and rscA genes was analyzed. The statistical significance was estimated by paired T-test. The observed biofilms were different for each particular strain. The biofilm formation was the highest in the rich medium (LB) after 24 h and its level hasn't changed in time. When biofilm level was compared to bacterial growth (relative biofilm) - it was higher in a minimal medium in comparison to enriched medium. These results suggest that most of the bacterial cells prefer to live in a biofilm community under the difficult environmental conditions. Moreover, biofilm formation on polyurethane surface did not correlate with biofilm formation on glass. It suggests that mechanisms of biofilm formation can be correlated with other bacterial properties. This phenomenon may explain different types of biofilm formation among one species and even one pathotype - uropathogenic Escherichia coli.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 765-771
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of multidrug resistant and extended spectrum β-lactamase (ESBL) - producing Escherichia coli in wastewater of two healthcare facilities in Ibadan, Nigeria
Autorzy:
Adekanmbi, Abimbola O.
Adeyemi, Adedoyin O.
Olajide, Oreoluwa M.
Powiązania:
https://bibliotekanauki.pl/articles/1076664.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Escherichia coli
hospital-waste
wastewater
β-lactamase
Opis:
Most industries in developing countries of the world, especially hospitals and other clinical settings, lack wastewater treatment facilities, and as such, untreated wastewater from their operations are discharged into water bodies without any form of treatment. This study aimed at the antibiotic sensitivity profile and ESBL production in E. coli isolated from untreated hospital wastewater before discharge into the environment. Untreated wastewater from two hospitals, a State Government-owned hospital (SGH) and a privately-owned hospital (POH) with no wastewater treatment facilities were sampled for a period of four months. Isolation of E. coli was carried out using the pour plate technique on Eosin Methylene Blue agar, while identification was carried out using conventional methods. Determination of ESBL production was done by means of the Double Disc Synergy Technique and antibiotic sensitivity testing was carried out by employing the disc diffusion method. A total of fifty-eight (58) E. coli were obtained: SGH at 55 and POH at 3. Herein, in 100% of the total count, resistance was indicated for ampicillin and ertapenem, while 14%, 11%, 16% and 57% of the total count were resistant to ceftazidime, cefpodoxime, cefotaxime and amoxicillin-clavulanate, respectively. In addition, 94.8% showed resistance to tetracycline, 19% to ciprofloxacin, 6.9% to gentamycin, 39.7% to chloramphenicol and 55% and 47% to sulfamethoxazole-trimethoprim and nalidixic acid, respectively. Furthermore, 94.8% of all the isolates were multidrug resistant (MDR), while 29.3% were ESBL positive. Wastewater from the two hospitals under study contained ESBL positive and MDR E. coli, suggesting a need to forestall a potential threat to public health by treating the wastewater generated by both hospitals before discharge into the environment.
Źródło:
World News of Natural Sciences; 2019, 26; 167-175
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Removal of Escherichia coli from Domestic Wastewater Using Electrocoagulation
Autorzy:
Aguilar-Ascon, Edwar
Powiązania:
https://bibliotekanauki.pl/articles/124695.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
domestic wastewater
electrocoagulation
escherichia coli
electrochemical disinfection
Opis:
The objective of this study was to evaluate the efficiency of electrocoagulation in the removal of Escherichia coli from domestic and urban wastewaters and to determine the effects of the main operational parameters on the process. An electrocoagulation reactor with aluminum and iron electrodes was built for this purpose. A factorial design was applied, where amperage, treatment time, and pH were considered as the factors and E. coli percent removal was the response variable. After 20 min of treatment, >97% removal efficiency was achieved. The highest E. coli removal efficiency achieved was 99.9% at a neutral pH of 7, amperage of 3 A, and treatment time of 60 min. However, the removal efficiency of close to 99% was also achieved at natural wastewater pH of 8.5. The statistical analyses showed that the three tested factors significantly affected the E. coli removal percentage (p < 0.05). These results indicate that electrocoagulation has a high disinfection power in a primary reactor in removing water contaminants as well as simultaneously removing pathogenic microorganisms when compared to biological treatment processes. This represents an additional benefit, because it will considerably reduce the use of chlorine during the final disinfection stage.
Źródło:
Journal of Ecological Engineering; 2019, 20, 5; 42-51
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Silver-clay nanohybrid as a coating for improvement of the antibacterial properties of paper
Autorzy:
Alikhah, Tahereh
Babavand, Arash
Afra, Elyas
Ghasemian, Ali
Saraeian, Ahmadreza
Powiązania:
https://bibliotekanauki.pl/articles/342609.pdf
Data publikacji:
2020
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Drewna
Tematy:
paper coating
silver-clay nanohybrid
antibacterial property
Escherichia coli
Staphylococcus aureus
Opis:
This comparative study was carried out to investigate the effect of nanosilver, silver-clay nanohybrid, and silver-milled clay nanohybrid coatings on the antibacterial characteristics of paper. Nanosilver (25 ppm) was used as a singleand double-layer coating. The results demonstrated that treatment with pure nanosilver was more efficient in the reduction of microbial growth. Also, milling of clay enabled better maintenance of silver nanoparticles, and led to a greater decrease in bacterial growth than in the case of the original silver-clay nanohybrid. Evaluation of the sustainability of antibacterial characteristics confirmed that, although pure nanosilver treatment achieved better performance in the first 15 minutes than nanohybrid samples, the performance of the nanohybrids improved with the passing of time. As expected, the treatments decreased the brightness of paper, while the opacity increased significantly; pure nanosilver treatment led to lower brightness than the others, and the opacity was higher in the case of the silver-clay nanohybrid than with the other treatments.
Źródło:
Drewno. Prace Naukowe. Doniesienia. Komunikaty; 2020, 62, 205; 109-124
1644-3985
Pojawia się w:
Drewno. Prace Naukowe. Doniesienia. Komunikaty
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wound healing potential of Capparis spinosa against cutaneous wounds infected by Escherichia coli in a rat model
Możliwość leczenia ran ciętych zainfekowanych Escherichia coli za pomocą Capparis spinosa na modelu szczurzym
Autorzy:
Asheghian Amiri, I.
Moslemi, H.R.
Tehrani-Sharif, M.
Kafshdouzan, K.
Powiązania:
https://bibliotekanauki.pl/articles/72825.pdf
Data publikacji:
2015
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
wound
cutaneous wound
Escherichia coli
wound infection
treatment
Capparis spinosa
plant extract
ethanol extract
rat
animal model
Opis:
Wound infection has become a major medical problem in recent years. This study was conducted to evaluate the healing activity of Capparis spinosa against surgical wounds infected by Escherichia coli. Twenty male rats were divided into two groups. Excisions were created surgically on the animals’ skin and then infected with E coli. Group 1 was treated with C spinosa while Group 2 was untreated. Wound biopsy specimens were collected on days 5, 10 and 16 and analyzed. Results showed that the hydroxyproline content in treatment group was significantly higher in various post wounding days. Protein content increased gradually in ten days. Results of histopathological studies showed moderate to intense granulation tissue formation in treated group on day 10. The histopathological studies showed, that the new epidermis in treated group was thicker than in control group on day 16 post wounding. The present study has demonstrated that ethanol extract of C spinosa includes properties that accelerate wound healing activities.
Infekcje ran stały się ostatnio dużym problemem medycznym. W prezentowanym artykule przedstawiono badania przeprowadzone w celu określenia działania Capparis spinosa przeciwko infekcjom ran pooperacyjnych przez Escherichia coli. Dwadzieścia szczurów płci męskiej podzielono na dwie grupy. Skórę zwierząt nacięto operacyjnie, a następnie ranę zainfekowano E coli. Grupę 1 leczono C spinosa, grupa 2 nie była leczona. Biopsję ran przeprowadzono 5., 10. i 16. dnia, a następnie poddano analizie. Wyniki wykazały, że zawartość hydroksyproliny w grupie leczonej była wyraźnie wyższa w poszczególnych dniach następujących po powstaniu rany. Zawartość białka rosła stopniowo przez 10 dni. Obserwacje histopatologiczne wskazywały na umiarkowaną do intensywnej granulację tkanki w grupie leczonej 10. dnia. Na ich podstawie stwierdzono również, że nowa epiderma w grupie leczonej była grubsza niż w grupie kontrolnej po 16 dniach od zranienia. Prezentowane badania wskazują, że wyciąg etanolowy z C spinosa ma właściwości przyspieszające gojenie się ran.
Źródło:
Herba Polonica; 2015, 61, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The expression level of a recombinant lipase predicted in silico by different codon optimization algorithms
Autorzy:
Bello, Adebayo J.
Igwo-Ezikpe, Miriam
Toye, Emuejevoke T.
Okpuzor, Joy
Powiązania:
https://bibliotekanauki.pl/articles/1046552.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
COOL
Codon optimization
Cohnella
Escherichia coli
ExpOptimizer
JCat
algorithm
in silico
lipase
presyndocon
protein expression
Opis:
Optimizing codons of gene of interest has offered a value-added way to increase heterologous expression of proteins. Different algorithms have been designed to achieve this purpose. These algorithms make use of parameters such as codon Adaptation index (CAI), codon context (CC), percentage guanine-cytosine (GC) content and RNA instability for predicting optimal codons responsible for increased protein expression. Lipase 3646 is an enzyme with great potential for industrial applications. This enzymes has been described to possess thermostable properties with relative stability in high alkaline pH and at different concentrations of organic solvents and inhibitors. This research therefore used JCat, Codon optimization online (COOL), presyndocon and ExpOptimizer algorithms to predict expression level of lipase 3646 enzyme in silico by optimizing its gene coding sequence. The results showed that there were variations in the CAI generated by the algorithms for the same 3646 DNA sequence which suggests that each algorithm is specific for its own generated CAI. COOL algorithm prediction based on other parameters showed good results for potential expression of the lipase. Thus, we recommend COOL algorithm for codon optimization of the lipase 3646 gene for industrial applications.
Źródło:
World Scientific News; 2019, 137; 31-41
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Guanylin and related peptides
Autorzy:
Beltowski, J.
Powiązania:
https://bibliotekanauki.pl/articles/69588.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
gastrointestinal tract
lymphoguanylin
uroguanylin
peptide
guanylin
Escherichia coli
cystic fibrosis
protein kinase G
enterotoxin
diarrhea
Źródło:
Journal of Physiology and Pharmacology; 2001, 52, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sery dojrzewające jako środowisko rozwoju patogenów
Ripened cheeses as pathogens growth environment
Autorzy:
Berthold, A.
Pluta, A.
Dolińska, M.
Powiązania:
https://bibliotekanauki.pl/articles/228836.pdf
Data publikacji:
2008
Wydawca:
Wyższa Szkoła Menedżerska w Warszawie
Tematy:
sery podpuszczkowe
zatrucia pokarmowe
Liseria monocytogenes
Salmonella spp.
Escherichia coli
Staphylococcus aureus
rennet cheeses
foot poisonings
Opis:
Celem artykułu jest omówienie możliwości rozwoju w serach podpuszczkowych dojrzewających drobnoustrojów patogennych, takich jak: Listeria monocytogenes, Escherichia coli. Salmonella sp. oraz Staphylococcus aureus. Przeanalizowano wpływ różnych czynników technologicznych wpływających na rozwój patogenów w serach. Dokonano przeglądu piśmiennictwa dotyczącego jakości mikrobiologicznej serów rynkowych. Przedstawiono przypadki zatruć pokarmowych wywołanych spożyciem serów dojrzęwająacych, zanieczyszczonych drobnoustrojami chorobotwórczymi.
The aim of the study was to review the possibilities of growth in rennet cheeses pathogenic microorganisms, such as: Listeria monocytogenes, Escherichia coli, Salmonella sp. and Staphylococcus aureus. The influence of some factors on the growth of pathogenic bacteria was analysed. The review of the literature concerning the microbiological quality of market cheeses was made. The cases of food pisonings, caused by consumption of rennet cheeses, contaminated with pathogenic bacteria were shown.
Źródło:
Postępy Techniki Przetwórstwa Spożywczego; 2008, 1; 79-84
0867-793X
2719-3691
Pojawia się w:
Postępy Techniki Przetwórstwa Spożywczego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Escherichia coli O104:H4 outbreak - have we learnt a lesson from it?
Autorzy:
Bloch, Sylwia
Felczykowska, Agnieszka
Nejman-Faleńczyk, Bożena
Powiązania:
https://bibliotekanauki.pl/articles/1039632.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
detection methods
Escherichia coli O104:H4
STEC
mobile genetic elements
outbreak
Opis:
Shiga toxin-producing Escherichia coli (STEC) strains belong to the group of pathogens that cause bloody diarrhea and hemorrhagic colitis with often severe complications. The main problem with human pathogenic E. coli strains, including STEC, is a wide spectrum of phenotypes and clinical manifestations. It is related to a variety of exchangeable genetic elements, like plasmids, bacteriophages, transposons and pathogenicity islands, that take part in horizontal gene transfer which influences creation of new dangerous bacterial strains. A good example of this phenomenon is a novel Shiga toxin-producing E. coli O104:H4 serotype that was associated with a widespread and severe foodborne disease outbreak in Germany in 2011. The O104:H4 strain was created by a number of horizontal gene transfer events between two distinct pathogens, resulting in the emergence of the new, atypical strain. That outbreak proved that also rare and unusual serotypes of STEC may be a significant risk factor and that the procedures recommended for STEC detection were not suitable to deal with this kind of pathogens. With respect to new combinations of chromosomal and extrachromosomal elements in susceptible bacterial hosts, epidemics and frequent human infections caused by STEC strains, we suggest that more attention should be paid to the development and improvement of diagnostic methods. It is difficult to determine STEC bacteria by general microbiological, biochemical and immunological assays, because strains can vary dramatically in their phenotypic and serotypic properties. It is postulated that standardized genetic tests, based on detection of features most frequently presented by STEC, particularly those located on easily exchangeable elements (such as Shiga toxin-encoding phages), can be more adequate for STEC detection.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 483-488
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zależność wzrostu B. coli na selektywnym podłożu od środowiska wodnego zdolność przeżywania B. coli w różnych środowiskach wodnych
The growth od B. coli on selective medium depending on water environment the ability of B. coli growth in various water environments
Autorzy:
Buczowska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/874786.pdf
Data publikacji:
1956
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
srodowisko wodne
wzrost bakterii
Escherichia coli
podloza
wody powierzchniowe
flora bakteryjna
aquatic environment
bacterial growth
substrate
surface water
bacterial flora
Źródło:
Roczniki Państwowego Zakładu Higieny; 1956, 07, 6
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence and Survivability of Escherichia coli and Enterococci in Waters Used as Bathing Areas
Występowanie i przeżywalność Escherichia coli i enterokoków w wodach wykorzystywanych jako kąpieliska
Autorzy:
Budzińska, K.
Szejniuk, B.
Michalska, M.
Berleć, K.
Powiązania:
https://bibliotekanauki.pl/articles/1813714.pdf
Data publikacji:
2018
Wydawca:
Politechnika Koszalińska. Wydawnictwo Uczelniane
Tematy:
bathing water
indicator bacteria
survivability
Escherichia coli
Enterococcus faecalis
woda kąpieliskowa
bakterie wskaźnikowe
przeżywalność
Opis:
Bathing waters during the holiday season are used with great frequency for recreational purposes. It should be noted that they are subject to microbiological contamination, and therefore pose a serious health risk to the people using them. In accordance with the above, study which was undertaken aimed at assessing the purity status of water used for bathing purposes and determining the survival time of Escherichia coli and Enterococcus faecalis bacteria in the test water. Water samples were taken from three bathing areas of the Koronowo Reservoir: Samociążek (batching area I), Pieczyska (bathing area II), Kręgiel (bathing area III) three times in the months of July, August and September. The number of indicator bacteria Escherichia coli and enterococci was determined in the water samples. Additionally, each time the samples were taken, the water temperature and the number of bathers were measured. The determination of the number of Escherichia coli and enterococci bacteria in the tested water samples was performed by membrane filtration. Two strains of Escherichia coli ATCC 25922 and Enterococcus faecalis ATCC 19433 were used in studies to determine the survival time. Changes in the number of indicator bacteria were determined by usage of MPN method (most probable number of bacteria). The analyzes indicated that the water tested was characterized by significant microbiological contamination, because in July the occurrence of E.coli bacteria was recorded at an average of 3697 cfu/100 ml (bathing area I) to 4539 cfu/100 ml (bathing area II). Additionally, in the samples of water taken from the bathing area III, the number of these bacteria was high and amounted to 3836 cfu/100 ml. However, in August the number of these bacteria decreased and ranged from 2062 cfu/100 ml (bathing area I) to 3327 cfu/100 ml (bathing area III). In the case of enterococci, it was found that the majority of these microorganisms were isolated from water from bathing area I in July and their number was 412 cfu/100 ml and from the bathing area III in August – 404 cfu/100 ml. In the remaining samples of the analyzed water, the number of enterococci didn’t exceed the normative value of 400 cfu/100 ml. It was indicated that in September the water from all the examined bathing areas met the microbiological requirements specified in the applicable legal acts. The obtained results proved that the number of indicator bacteria was determined by the temperature of water and the number of bathers. In accordance to regression equations, it was found that Escherichia coli and Enterococcus faecalis lasted longer in water at temperature of 4°C respectively: 45 and 52 days, while at temperature of 20°C their survival time was 52 and 26 days. The study indicated the necessity to perform permanent sanitary control of water used for bathing purposes in the aspect of epidemiological threats.
Wody kąpieliskowe w sezonie wakacyjnym wykorzystywane są z dużą częstotliwością w celach rekreacyjnych. Należy podkreślić, że ulegają one zanieczyszczeniu mikrobiologicznemu, w związku z czym stanowią poważne zagrożenie zdrowotne dla korzystających z nich osób. W związku z powyższym podjęto badania, których celem była ocena stanu czystości wody wykorzystywanej w celach kąpieliskowych oraz ustalenie w badanej wodzie czasu przeżywalności bakterii Escherichia coli i Enterococcus faecalis. Próbki wody pobierano z trzech kąpielisk Zalewu Koronowskiego: Samociążek (kąpielisko I), Pieczyska (kąpielisko II), Kręgiel (kąpielisko III) trzykrotnie w miesiącach lipcu, sierpniu oraz we wrześniu. W próbkach wody oznaczono liczbę bakterii wskaźnikowych Escherichia coli i enterokoków. Dodatkowo przy każdym poborze próbek dokonywano pomiaru temperatury wody oraz liczbę kąpiących się osób. Oznaczenie liczby bakterii Escherichia coli oraz enterokoków w badanych próbkach wody przeprowadzono metodą filtracji membranowej. W badaniach dotyczących ustalenia czasu przeżywalności wykorzystano dwa szczepy Escherichia coli ATCC 25922 oraz Enterococcus faecalis ATCC 19433. Zmiany liczebności bakterii wskaźnikowych oznaczono metodą NPL (najbardziej prawdopodobna liczba bakterii). Przeprowadzone analizy wykazały, że badana woda charakteryzowała się znacznym zanieczyszczeniem mikrobiologicznym, ponieważ w miesiącu lipcu odnotowano występowanie bakterii E. coli średnio na poziomie od 3697 cfu/100 ml (kąpielisko I) do 4539 cfu/100 ml (kąpielisko II). Ponadto w próbkach wody pobranych z kąpieliska III liczba tych bakterii była wysoka i wynosiła 3836 cfu/100 ml. Z kolei w sierpniu liczba tych bakterii zmniejszyła się i kształtowała się w zakresie od 2062 cfu/100 ml (kąpielisko I) do 3327 cfu/100 ml (kąpielisko III). W przypadku enterokoków stwierdzono, że najwięcej tych drobnoustrojów wyizolowano z próbek wody z kąpieliska I w lipcu, liczba ich wynosiła 412 cfu/100 ml oraz z III kąpieliska w miesiącu sierpniu – 404 cfu/100 ml. W pozostałych próbkach analizowanej wody liczba enterokoków nie przekroczyła wartości normatywnej 400 cfu/100 ml. Wykazano, że w miesiącu wrześniu woda ze wszystkich badanych kąpielisk spełniała wymagania mikrobiologiczne określone w obowiązujących aktach prawnych. Uzyskane wyniki dowiodły, że liczba bakterii wskaźnikowych determinowana była temperaturą wody i liczbą kąpiących się osób. Na podstawie równań regresji ustalono że bakterie Escherichia coli i Enterococcus faecalis dłużej przeżywały w wodzie o temperaturze 4°C odpowiednio: 45 i 52 dni, natomiast w temperaturze 20°C czas ich przetrwania wynosił 52 i 26 dni. Przeprowadzone badania wskazują na konieczność ciągłego monitoringu wody wykorzystywanej w celach kąpieliskowych w aspekcie zagrożeń epidemiologicznych.
Źródło:
Rocznik Ochrona Środowiska; 2018, Tom 20, cz. 1; 309-325
1506-218X
Pojawia się w:
Rocznik Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli
Autorzy:
Bzdzion, Lukasz
Krezel, Hanna
Wrzeszcz, Karol
Grzegorek, Irmina
Nowinska, Katarzyna
Chodaczek, Grzegorz
Swietnicki, Wieslaw
Powiązania:
https://bibliotekanauki.pl/articles/1038684.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Escherichia coli (E. coli)
type III secretion system (T3SS)
enzyme inhibitor
small molecule
Opis:
Enteropathogenic E. coli (EPEC) is a human pathogen using type III secretion system for delivery of proteins directly into the human host. The system contains a single ATPase, EscN, which is essential for uncoupling of proteins from their complexes with chaperones before the delivery. The structure of EscN ATPase (PDB code: 2obm) was used to screen computationally for small molecule inhibitors blocking its active site. Two lead candidates were examined but only one, Compound 54, was selected for further optimization. After extended QSAR optimization, two derivatives were found to be competitive inhibitors of EscN capable of blocking ATPase activity with a Ki below 50 µM. One candidate, WEN05-03, with a Ki=16±2 µM, was also minimally toxic to mammalian cells as determined by other assays. In the cell infection model of HeLa cells with EPEC, Compound WEN05-03 completely blocked actin cluster formation at 100 µM concentration, when analyzed by confocal microscopy. The second best inhibitor of EscN ATPase activity was WEN04-34 with a Ki=46±2 µM. However, the compound was highly toxic to the BALB/3T3 cell line. In summary, the work identifies a compound blocking bacterial ATPase in its active site without causing cellular toxicity to the host cells. It is the first report showing feasibility of using bacterial virulence system ATPase as a target for safe, non-toxic compounds and offering a proof-of-concept for non-antibiotic alternatives.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 49-63
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics of E.coli Nissle 1917 in response to Cocos nucifera sap and wine
Autorzy:
Chandrasekhar, K
Pramoda Kumari, J.
Powiązania:
https://bibliotekanauki.pl/articles/11862.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
proteomics
Escherichia coli
plant response
coconut palm
Cocos nucifera
sap
wine
protein
probiotic
identification
scanning
Opis:
In the present study, we described the protein profile experimentally by 2D-PAGE and MALDI analysis to understand the stress mechanisms of cocoti sap and wine on E.coli Nissle 1917. We isolated one newly expressed protein from cocoti wine treated gel which is not present in both control and cocoti sap treated sample i.e. P21 prophage-derived head-stabilizing proteinVG03_ECOL6 (3n1) also called as Head protein gp3. This protein mainly activities related to the viral life cycle. It helps to attach the viral gene into host. The growth rate was delayed in cocoti wine treated E.coli Nissle 1917 when compared to control and cocoti sap treated samples. Stress mechanism induce many proteins they are involved in metabolic process, hydrolase activity, lyase activity, quinone binding, phosphotransferase system, carbohydrate metabolism, DNA binding, DNA repair, transferase activity, oxidoreductase, purine metabolism, transcription antitermination, transcription regulation and other related activities. We proved that the predicted protein structure quality, resolution, density and error plot values by QMEAN analysis. Based on these results, only two differentially expressed proteins under sap stress showed that the significant results, which were N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1, PTPB1_ECOLI and DinI-like protein Z3305/ECs2939 in prophage CP-933VDINI1_ECO57. In case of wine stress, the differentially expressed proteins were Transcription anti-termination protein RFAH- ECO57 NusA and PUR7- eco24- phosphoribosylamidazole-succinocarboxamide synthase showed significant results. ProtParam analysis indicating that the multiple physico-chemical characters of differentially expressed proteins were differed and compared. The phylogenetic tree represents the relationship in-between the differentially expressed proteins, were showed siblings (related) as well as monophytic clade.
Źródło:
International Letters of Natural Sciences; 2015, 41
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial spectrum of four compounds from yeasts in koumiss
Autorzy:
Chen, Y.J.
Du, C.G.
Guo, Y.Q.
Zhao, Y.F.
Aorigele, C.
Wang, C.J.
Simujide, H.
Aqima, W.
Zhang, X.Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087160.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Kluyveromyces marxianus
Saccharomyces cerevisiae
Escherichia coli
minimum inhibition concentration
minimum bactericidal concentration
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 167-173
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil
Autorzy:
Dąbrowski, Michał
Sienkiewicz, Monika
Zielińska-Bliźniewska, Hanna
Dąbrowska, Marta
Seredyńska, Małgorzata
Kochan, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/763827.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
Tematy:
Escherichia coli, clove oil, urinary tract infections
Opis:
Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium, which is a primary cause of urinary tract infections. Resistance to antibiotics has become a particular problem in recent decades. Consequently, there is an unmet need for new therapeutic options. It has been observed that essential oils have bactericidal effects. The antimicrobial susceptibility testing for Escherichia coli isolates obtained from urine of patients with urinary tract infections was determined via disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2015). Essential oil from clove – Syzygium aromaticum (L.) Merill et L.M. (Myrtaceae) was analyzed by GC-FID-MS. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were detected by using the micro-dilution broth method. Escherichia coli clinical isolates are characterized by high resistance to ampicillin, amoxicillin with clavulanic acid, norfloxacin, trimethoprim/sulfamethoxazole, tetracycline, tobramycin and ticarcillin. Clove oil possesses strong inhibiting and killing properties against E. coli isolates, among them the ones resistant to recommended antibiotics. The results of this study highlight the need for testing the efficacy of new agents to inactivate bacteria in clinical settings.
Źródło:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2016, 71, 2
2083-3563
0066-2232
Pojawia się w:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 116

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