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    Tytuł:
    Antimicrobial Potency from Endophytic Bacteria of Bignay Plant (Antidesma bunius (L.) Spreng.) Against Pathogenic Bacteria
    Autorzy:
    Indrawati, Ida
    Rosiana, Nia
    Rizki, Andita Fitri Mutiara
    Nada, Nabila
    Powiązania:
    https://bibliotekanauki.pl/articles/1031544.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antibacteria
    Antidesma bunius
    Endophytic
    Gram stain
    Opis:
    The need for new drugs is increasing in the antibiotic field. However, synthetic antibiotics often have a bad impact on body health, hence, in this paper, endophytic bacteria is investigated as an antibiotic source. The purpose of this research is to gain an understanding of the antibacterial potency of the endophytic bacteria associated with the Bignay plant (Antidesma bunius) against pathogenic bacteria that is Gram-negative i.e Salmonella thypimurium ATCC 14028, Escherichia coli ATCC 35218 and Gram-positive i.e Staphyllococcus aureus ATCC 6538. This research used the descriptive method and consists of endophytic bacterial isolation, identification, and antimicrobial testing. The endophytic bacterial isolation from fruit and stem of Bignay plant was performed by using the pour plate method, while the bacteria was identified by applying a biochemical test method using Vitek 2.0. The antimicrobial test was performed by way of paper disc diffusion. The resulting endophytic bacteria isolated from the fruit and stem of the Bignay plant are Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus sp.1, and Bacillus sp.2. The most potential endophytic bacteria as antimicrobial against pathogenic bacteria were found to be Bacillus pumilus, Bacillus sp.1, and Bacillus sp.2.
    Źródło:
    World News of Natural Sciences; 2020, 31; 1-8
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Endofity bakteryjne drzew leśnych - stan wiedzy i sposoby wykorzystania
    Endophytic bacteria of the forest trees - state of the art and possible applications
    Autorzy:
    Siebyła, M.
    Szyp-Borowska, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/979123.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    endophytic bacteria
    forest trees
    host plant
    symbiosis
    Opis:
    The interaction of endophytic microorganisms with plants are a common occurrence that brings mutual benefits to partners. Plants are the main habitat of bacteria that live inside their tissues and do not cause disease symptoms, but affect the growth and development of plants by producing substances that promote their development. Research on the diversity of endophytic bacteria associated with forest trees is insufficient – little is known about the diversity of endophytic bacteria, and especially their function in tree tissues. Bacteria positively affecting the host tree, among others, increase biomass growth by supporting tree health. The species diversity of endophytic bacteria in plants is influenced by the plant genotype, tissue type, development phase and environmental conditions. So far, bacteria that develop in root, stem, and leaf tissues have been best known. Among the forest trees in which the occurrence and diversity of endophytic bacteria have been studied, there are species such as pine, spruce, birch and oak. The presented paper is a review of the latest literature on the subject.
    Źródło:
    Sylwan; 2020, 164, 06; 497-504
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Endophytes – untapped resources and pharmacological prospects against coronaviruses
    Autorzy:
    Erhirhie, Earnest Oghenesuvwe
    Ezeagha, Chigozie Celestina
    Okafor, Glory Chiamaka
    Ikegbune, Chidozie
    Mohammad, Mukim
    Powiązania:
    https://bibliotekanauki.pl/articles/2211815.pdf
    Data publikacji:
    2023-03-25
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    coronaviruses
    endophytes
    endophytic fungi
    SARS-CoV
    natural products
    Opis:
    Introduction and aim. Viral infections stand to be among the most devastating diseases globally. Though significant efforts have been made in research and drug development against viral infections, the search for safe, affordable and effective vaccines against the current ravaging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still on. This is because already approved vaccines still need improvement. This review draws the attention of researchers on the potentials of bioactive substances from endophytes against the novel coronaviruses. Material and methods. This assessment was made using references of articles published in English peer reviewed journals indexed in PubMed and Google Scholars databases up to June, 2022. The following key words were used; ‘coronaviruses’, ‘Endophytes’, ‘Endophytes and viral infections, ‘Endophytes and COVID-19, ‘SARS-CoV’. Analysis of the literature. In-silico, in-vitro and in-vivo studies revealed that natural compounds from endophytes showed antiviral activities against various human coronavirus, including HCoV 229E and a norovirus surrogate, the feline coronavirus FCV F9, COVID-19, Coronavirus 2 (SARS-CoV-2), SARSCoV-2 Mpro, among others. Conclusion. This finding calls for researchers to also focus on endophytes, as part of drugs development in the bid to finding possible solution in combating the devastating COVID-19, an emerging situation.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2023, 1; 145-151
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Siderophore Production of the Hg-Resistant Endophytic Bacteria Isolated from Local Grass in the Hg-Contaminated Soil
    Autorzy:
    Ustiatik, Reni
    Nuraini, Yulia
    Suharjono, -
    Handayanto, Eko
    Powiązania:
    https://bibliotekanauki.pl/articles/1838397.pdf
    Data publikacji:
    2021
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    endophytic bacteria
    growth promoter
    metal chelator
    siderophore production
    Hg phytoremediation
    Opis:
    Mercury (Hg)-contaminated soil remediation has become an urgent necessity due to its harmful effect on the environment and living organisms. The use of plant-endophyte partnership for phytoremediation demonstrates an excellent opportunity for cleaning heavy metal contaminated soil. This study aimed to screen and characterize the phenotype of the Hg-resistant endophytic bacteria from local grasses (Cynodon dactylon and Eleusine indica) in the Hg-contaminated soil of West Nusa Tenggara, Indonesia with siderophore-producing traits. Siderophore production of bacteria was qualitatively tested using overlay-chrome azurol S (O-CAS) medium and quantitatively tested using the succinic acid medium. The assay was designed using a Completely Randomized Factorial Design consisting of two factors, i.e., isolate type and incubation time with three replicates. The selected isolates were pathogenicity tested, then they were phenotypically characterized. All tested isolates showed a positive result on changing O-CAS medium color from blue to yellow/brown that indicated hydroxamate type of siderophore. The highest siderophore production was achieved at 72 hours of incubation, by the EI5 and EI6 isolates (62.90% and 35.31%, respectively). In turn, the CD6, EI5 and EI6 isolates achieved high siderophore production at a short incubation period (48 hours). However, during the hemolysis test, only the CD6 and EI6 isolates were not pathogenic. The CD6 and EI6 isolates would be used for phytoremediation on Hg-contaminated soil in the future study. On the basis of the 16S rDNA analysis, it was shown that the CD6 isolate was Jeotgalicoccus huakuii and the EI6 isolate was Bacillus amyloliquefaciens.
    Źródło:
    Journal of Ecological Engineering; 2021, 22, 5; 129-138
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genomic Analysis of Plant-Associated Bacteria and Their Potential in Enhancing Phytoremediation Efficiency
    Autorzy:
    Piński, A.
    Hupert-Kocurek, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/125404.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    endophytic bacteria
    rhizospheric bacteria
    Stenotrophomonas maltophilia
    heavy metals
    phytoremediation
    xenobiotics
    Opis:
    Phytoremediation is an emerging technology that uses plants in order to cleanup pollutants including xenobiotics and heavy metals from soil, water and air. Inoculation of plants with plant growth promoting endophytic and rhizospheric bacteria can enhance efficiency of phytoremediation. Genomic analysis of four plant-associated strains belonging to the Stenotrophomonas maltophilia species revealed the presence of genes encoding proteins involved in plant growth promotion, biocontrol of phytopathogens, biodegradation of xenobiotics, heavy metals resistance and plant-bacteria-environment interaction. The results of this analysis suggest great potential of bacteria belonging to Stenotrophomonas maltophilia species in enhancing phytoremediation efficiency.
    Źródło:
    Journal of Ecological Engineering; 2017, 18, 4; 152-159
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Epicoccum nigrum link as a potential biocontrol agent against selected dermatophytes
    Autorzy:
    Piecuch, Agata
    Ogórek, Rafał
    Dyląg, Mariusz
    Cal, Magdalena
    Przywara, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/11543325.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Epicoccum nigrum
    marine environment
    Epicoccum purpurascens
    endophytic fungi
    secondary metabolite
    fungi
    toxicity
    Opis:
    Epicoccum nigrum Link is well known for producing biologically-active substances with activities against prokaryotic and eukaryotic cells. The major goal of this study was to assess E. nigrum as a potential in vitro agent against selected species of dermatophytes. The effects of the types of media used in this study on the interactions between the microscopic fungi were also examined. Epicoccum nigrum’s bioactive metabolites exhibited a strong growth inhibitory effect against the dermatophytes, suggesting its potential as a biocontrol agent. Notably, the strength of these interactions was dependent on the type of the medium. These secondary metabolites are not toxic against the higher eukaryotic organisms, which was further demonstrated by using the Galleria mellonella model.
    Źródło:
    Acta Mycologica; 2020, 55, 1; 5516
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study of endophytic fungi in timothy (Phleum pratense) in Estonia
    Autorzy:
    Varvas, T.
    Kasekamp, K.
    Kullman, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/67480.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    endophytic fungi
    fungi
    timothy
    Phleum pratense
    agricultural grass
    grass
    isolate identification
    Estonia
    Opis:
    Timothy (Phleum pratense L.) is an important agricultural grass in Europe and North America, but there is little research into the occurrence and abundance of fungal endophyte species associated with this grass. The aim of this study was to identify fungal endophytes living within P. pratense and to determine if additional moisture applied during the growing season increases the diversity of endophytic fungi. We studied 58 isolates obtained from surface-sterilised blades of 60 P. pratense plants collected from Rõka Free Air Humidity Manipulation experimental plots (FAHM), Estonia. Morphological and molecular methods were used for isolate identification. As a result, 45 strains from 10 different taxa were identified, all belonging to Ascomycota. Five species were found to be new to P. pratense.
    Źródło:
    Acta Mycologica; 2013, 48, 1
    0001-625X
    2353-074X
    Pojawia się w:
    Acta Mycologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cichorin A: a benzo-isochromene from Nypa fruticans endophytic fungus Pestalotiopsis sp.
    Cychoryna A: benzo-izochromen z Pestalotiopsis sp. - endofitycznego grzyba Nypa fruticans
    Autorzy:
    Alade, G.O.
    Moody, J.O.
    Awotona, O.R.
    Lai, D.
    Adesanya, S.A.
    Proksch, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/71140.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    fungi
    cichorin A
    benzo-isochromene
    Nypa fruticans
    endophytic fungi
    Pestalotiopsis
    bioactive compound
    Opis:
    Introduction: Similar bioactive metabolites are obtainable from host plants as well as the endophytic fungi residing in them. Objective: The aim of the study is to isolate the major compound(s) from the endophytic fungus residing in Nypa fruticans Wurmb, Arecaceae family. Methods: Purification of the ethyl acetate extract of the isolated endophytic fungus was performed by employing different chromatographic techniques and structural elucidation of the isolated compound was carried out using UV and NMR spectroscopic methods. Results: Cichorin A was isolated from the ethyl acetate extract of the solid rice cultures of Pestalotiopsis sp., isolated from N. fruticans, collected in Nigeria. Conclusions: This compound is being isolated for the first time from a fungus; it is commonly isolated from the plant Cichorium intybus L. (Compositae).
    Wstęp: Z roślin – gospodarzy oraz z ich endofitycznych grzybów można otrzymać podobne aktywne metabolity. Cel: Celem prowadzonych badań było wyizolowanie głównego związku/związków z endofitów grzybowych zasiedlających nipę krzewinkową (palmę krzaczastą) Nypa fruticans Wurmb (Arecaceae). Metody: Oczyszczenie ekstraktu z izolowanych endofitów grzybowych, otrzymanego za pomocą octanu etylu, przeprowadzono za pomocą różnych technik chromatograficznych . Wyjaśnienie struktury wyizolowanego związku przeprowadzono metodą spektoskopii NMR. Wyniki: Z ekstraktu (otrzymanego za pomocą octanu etylu) z kultur Pestalotiopsis sp. (izolowanych z N. fruticans z Nigerii), hodowanych na pożywkach stałych ryżowych, otrzymano cychorynę A. Wnioski: Związek ten, powszechnie izolowany z rośliny Cichorium intybus L. (Compositae), po raz pierwszy został wyizolowany z grzybów.
    Źródło:
    Herba Polonica; 2017, 63, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of pathogenic and endophytic fungi and their influence on quality of medicinal plants applied in management of neurological diseases and mental disorders
    Występowanie patogenicznych i endofitycznych grzybów i ich wpływ na jakość roślin zielarskich stosowanych w leczeniu chorób neurologicznych i psychicznych
    Autorzy:
    Wielgusz, K.
    Irzykowska, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/72740.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    occurrence
    pathogenic fungi
    endophytic fungi
    plant quality
    medicinal plant
    neurological disease
    mental disorder
    phytotherapy
    human disease
    therapy
    mycotoxin
    human health
    Opis:
    Due to increasing demand of medicinal plants (MPs), quality and safety more attention to the plant health should be paid. Among herb pathogens, especially fungi cause serious diseases in these plants decreasing yield and quality of herbal raw material. Some species, i.e. Fusarium sp., Alternaria sp., Penicillium sp. are known as mycotoxin producers. Paradoxically, self-treatment with herbal raw material can expose the patient to mycotoxin activity. In tissues of some MPs species, asymptomatically endophytic fungi residue. It is known that they are able to influence a biosynthesis of secondary metabolites in their host plant or produce biologically active compounds. Until recently these microorganisms have been neglected as a component of MPs, the reason why there have unexplored bioactivity and biodiversity. The paper presents an overview of herbal plants that are used in the treatment of nervous system diseases. Pathogenic fungi that infect these plants are described. It focused mainly on species producing harmful mycotoxins. The publication presents a list of these mycotoxins and a brief description of their effects on human health. The second part of this article provides information on the occurrence of endophytic fungi in herbal plants and their effects on human health. Coexistence of fungi and medicinal plants is not fully understood but can be crucial to ensure health and safety of patients with neurological diseases and mental disorders.
    Ze względu na stały wzrost zapotrzebowania na jakość i bezpieczeństwo roślin zielarskich, należałoby zwrócić większą uwagę na ich zdrowotność. Wśród patogenów roślin zielarskich, szczególnie grzyby powodują poważne choroby, zmniejszając plon i jakość surowców roślinnych. Niektóre gatunki m.in. Fusarium sp., Alternaria sp., Penicillium sp. znane są jako producenci toksycznych metabolitów. Paradoksalnie ludzie próbujący leczyć się samodzielnie (używając surowców zielarskich), mogą być narażeni na działanie tych mykotoksyn. W tkankach niektórych roślin zielarskich występują (bezobjawowo) pozostałości grzybów endofitycznych. Wiadomo, że mają one zdolność wywierania wpływu na biosyntezę wtórnych metabolitów rośliny żywicielskiej lub wytwarzają związki biologicznie czynne. Do niedawna te mikroorganizmy były przeoczane jako „składnik” roślin zielarskich, co spowodowało, że nadal są polem niezbadanej bioaktywności i bioróżnorodności. W artykule przedstawiono przegląd roślin zielarskich stosowanych w leczeniu chorób układu nerwowego. Opisano grzyby patogeniczne dla tych roślin. Skupiono się głównie na gatunkach biosyntetyzujących szkodliwe mykotoksyny. Publikacja obejmuje listę tych mykotoksyn oraz krótki opis ich wpływu na zdrowie człowieka. Druga część artykułu dostarcza informacji o występowaniu grzybów endofitycznych w roślinach zielarskich i wpływie endofitów na zdrowie człowieka. Współistnienie grzybów i roślin leczniczych nie jest w pełni zrozumiane, a może być bardzo istotne dla zapewnienia zdrowia i bezpieczeństwa pacjentów z zaburzeniami neurologicznymi i psychicznymi.
    Źródło:
    Herba Polonica; 2017, 63, 4
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
    Autorzy:
    Allegrucci, N.
    Velazquez, M.S.
    Russo, M.L.
    Perez, E.
    Scorsetti, A.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/65417.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    endophytic fungi
    tomato
    entomopathogen
    fungi
    Beauveria bassiana
    inoculation method
    tomato leafminer
    Tuta absoluta
    Lepidoptera
    Gelechiidae
    biological control
    Solanum lycopersicum
    Opis:
    Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 4
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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