Temat: DNA polymerase - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR
Autorzy:: Trzewik, A.
Nowak, K.J.
Orlikowska, T.
Powiązania:: https://bibliotekanauki.pl/articles/66480.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: simple method
DNA extraction
rhododendron
leaf
plant infection
Phytophthora
polymerase chain reaction
detection
real-time PCR method
Opis:: Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.
Źródło:: Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)
Autorzy:: Tarach, Piotr
Powiązania:: https://bibliotekanauki.pl/articles/1830648.pdf
Data publikacji:: 2021-09-29
Wydawca:: Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:: nucleotide polymorphisms
DNA analysis
polymerase chain reaction
Opis:: Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of digested fragments; and (4) visualisation. Despite its obsolescence and the presence of high-throughput DNA analysis techniques, it is still applied in the analysis of SNPs associated with disease entities and in the analysis of genetic variation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). RFLP-PCR is a low-cost and low-throughput research method allowing for the analysis of SNPs in the absence of specialised equipment, and it is useful when there is a limited budget.
Źródło:: Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2021, 17; 48-53
1730-2366
2083-8484
Pojawia się w:: Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: The expression of UVR3 and two putative photolyases, PHR2 and At4g25290, is regulated by light
Autorzy:: Sztatelman, O.
Labuz, J.
Banas, A.K.
Powiązania:: https://bibliotekanauki.pl/articles/80793.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
DNA strand
RNA polymerase
DNA polymerase
replication
transcription
light regulation
putative photolyase
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Epi-genes potentiate plant biodiversity
Autorzy:: Szopa, J.
Powiązania:: https://bibliotekanauki.pl/articles/951285.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biodiversity
oligonucleotide
DNA methylation
gene expression
protein
methylase
polymerase
RNA polymerase
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: DNA isolation from Cryptosporidium oocysts directly from stool samples for diagnostic goals using PCR
Autorzy:: Sulima, P.
Powiązania:: https://bibliotekanauki.pl/articles/840154.pdf
Data publikacji:: 1998
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: diagnostic goal
stool
polymerase chain reaction
Cryptosporidium
DNA
oocyst
Źródło:: Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Polymorphism of DNA in nematodes of genus Trichinella Railliet, 1895 according to the data of polymerase chain reaction
Autorzy:: Shendrick, A.
Benedictov, I.
Bessonov, A.
Powiązania:: https://bibliotekanauki.pl/articles/838885.pdf
Data publikacji:: 1998
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: polymorphism
Trichinella pseudospiralis
Trichinella
nematode
polymerase chain reaction
DNA
Trichinella spiralis
Źródło:: Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia
Autorzy:: Sawicki, J.
Szczecinska, M.
Powiązania:: https://bibliotekanauki.pl/articles/57748.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: Acutifolia
random amplified polymorphic DNA
Sphagnum
genetic similarity
molecular identification
molecular marker
polymerase chain reaction
genetic relationship
species identification
peat moss
chloroplast
nuclear genome
Opis:: RAPDs, ISJs, ISSRs, ITS and katGs were applied to determine genetic relationships between common Sphagnum species of the section Acutifolia. Twenty populations were genotyped using ten ISJ primers, 12 pairs of katG primers, 10 ISSR and 10 RAPD primers, and a restriction analysis of ITS1 and ITS2. ISSR and katG markers revealed the greatest number of species-specific bands. An analysis of ITS1 and ITS2 regions with restriction enzymes also proved to be a highly effective tool for species identification.
Źródło:: Acta Societatis Botanicorum Poloniae; 2011, 80, 3
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
Autorzy:: Sadowski, J
Rorat, T
Cooke, R
Delseny, M
Powiązania:: https://bibliotekanauki.pl/articles/2046684.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: beta-amylase
rye
gene promoter
homology
amino acid
DNA cloning
prolamin
Secale cereale
mRNA
embryogenesis
endosperm
cDNA
inverse polymerase chain reaction
Opis:: The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 241-251
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Inhibition of poly(ADP-ribose) polymerase activity affects its subcellular localization and DNA strand break rejoining
Autorzy:: Ryabokon, Nadezhda
Cieślar-Pobuda, Artur
Rzeszowska-Wolny, Joanna
Powiązania:: https://bibliotekanauki.pl/articles/1040571.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: DNA strand break rejoining
efficiency of PARP inhibition
PARP foci
poly(ADP-ribose) polymerase (PARP)
PARP inhibitors
Opis:: Poly(ADP-ribose) polymerase (PARP) plays a crucial role in DNA repair. Modulation of its activity by stimulation or inhibition is considered as a potentially important strategy in clinical practice, especially to sensitize tumor cells to chemo- and radiotherapy through inhibition of DNA repair. Here we studied the effect of the three PARP inhibitors, 5-iodo-6-amino-benzopyrone (INH2BP), 1,5-isoquinolinediol (1,5-dihydroxyisoquinolinediol (1,5-IQD) and 8-hydroxy-2-methylquinazolin-4-[3H]one (NU1025), and for two of them the efficiency in slowing the rejoining of DNA strand breaks induced by H2O2 was compared. Inhibition of PARP changed its intranuclear localization markedly; cells exposed to the inhibitor NU1025 showed a significant tendency to accumulate PARP in large foci, whereas in untreated cells its distribution was more uniform. The speed and efficiency of rejoining of H2O2-induced DNA strand breaks were lower in cells incubated with a PARP inhibitor, and the kinetics of rejoining were modulated in a different manner by each inhibitor. At a concentration of 100 µM the efficiency of the inhibitors could be ranked in the order NU1025 > IQD > INH2BP. The two first compounds were able to decrease the overall PARP activity below the level detected in control cells, while INH2BP showed up to 40% PARP activity after exposure to H2O2.
Źródło:: Acta Biochimica Polonica; 2009, 56, 2; 243-248
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Oral cavity as permanent reservoir of Helicobacter pylori and potential source of reinfection
Autorzy:: Pytko-Polonczyk, J
Konturek, S.J.
Karczewska, E.
Bielanski, W.
Kaczmarczyk-Stachowska, A.
Powiązania:: https://bibliotekanauki.pl/articles/70310.pdf
Data publikacji:: 1996
Wydawca:: Polskie Towarzystwo Fizjologiczne
Tematy:: peptic ulcer
reinfection
dental plaque
gingival pocket
duodenal ulcer
urea breath test
saliva
stomach
DNA
Helicobacter pylori
oral activity
polymerase chain
Źródło:: Journal of Physiology and Pharmacology; 1996, 47, 1
0867-5910
Pojawia się w:: Journal of Physiology and Pharmacology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Adaptor-mediated amplification of minute amounts of severely fragmented ancient nucleic acids
Autorzy:: Pusch, C M
Blin, N.
Broghammer, M.
Nicholson, G.J.
Scholz, M.
Powiązania:: https://bibliotekanauki.pl/articles/2042022.pdf
Data publikacji:: 2001
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: mitochondrial DNA
genetics
nucleic acid
ancient DNA
polymerase chain reaction
DNA
cDNA
sex typing
Źródło:: Journal of Applied Genetics; 2000, 41, 4; 303-315
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Unidirectional orientation of twelve expressed tagged sites within 4o kb of human chromosomal region 22q13.1
Autorzy:: Pusch, C
Wang, Z.
Roe, B.
Blin, N.
Powiązania:: https://bibliotekanauki.pl/articles/2048293.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: RNA
human chromosome
chromosome
hybridization
genetic change
linear map
polymerase chain reaction
DNA
cosmid
cDNA
transcriptional orientation
Opis:: The single copy sequence D22S16 from human chromosomal region 22q13.1 that carries a putative conserved gene, was used to probe a chromosome 22-specific cosmid library. Genomic sequencing of one positive, 40 kb long cosmid (C1155) revealed a hereto unmapped gene (a subunit of DNA-dependent RNA polymerase II, POLR2F), a SOX9-related sequence and 12 expressed sequence tags. Although not parts of one consecutive gene, all 12 ESTs and, in addition, the polymerase gene are oriented in the same transcriptional direction within the genomic sequence represented by cosmid C1155.
Źródło:: Journal of Applied Genetics; 1998, 39, 2; 199-204
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Description of DNA analysis techniques and their application in oat (Avena L.) genome research
Charakterystyka technik analiz DNA oraz ich wykorzystanie w badaniach owsa (Avena L.)
Autorzy:: Okon, S.
Kowalczyk, K.
Powiązania:: https://bibliotekanauki.pl/articles/26845.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: DNA analysis technique
application
oat
Avena
genome
molecular marker
crossbreeding efficiency
genetic map
hexaploid
diploid
plant species
crown rust
powdery mildew
plant resistance
DNA marker
polymerase chain reaction
Opis:: DNA markers are used not only to estimate genetic similarity and distance but also to select and identify desirable forms, to assess the adjustment of breeding material, to confirm crossbreeding efficiency, to determine seed purity, and to identify the genes which determine important functional traits. In the case of oat, DNA markers were used to construct and increase the density of genetic maps both in hexaploid and diploid species. The development of markers for some important traits provides a fast selection of genotypes containing dwarf genes as well as the resistance genes to crown rust and powdery mildew. Numerous analyses of genetic similarity between different species belonging to the genus Avena which are currently carried out may contribute to explaining the process of evolution within this genus and may also explain the development of particular species of oat.
Markery DNA znalazły zastosowanie nie tylko w ocenie podobieństwa lub dystansu genetycznego, ale również w selekcji i identyfikacji pożądanych form, ocenie wyrównania materiałów hodowlanych, potwierdzaniu skuteczności krzyżowań, ocenie czystości materiału siewnego czy też do identyfikacji genów, warunkujących ważne cechy użytkowe. U owsa posłużyły one między innymi do konstrukcji i zagęszczenia map genetycznych zarówno gatunków heksaploidalnych jak i diploidalnych. Opracowanie markerów dla niektórych cech użytkowych pozwala na szybką selekcję genotypów zawierających geny karłowatości, odporności na rdzę koronową czy mączniaka prawdziwego. Natomiast prowadzone liczne analizy podobieństwa genetycznego różnych gatunków z rodzaju Avena mogą przyczynić się do wyjaśnienia ewolucji w obrębie tego rodzaju jak również mogą wyjaśnić powstawanie poszczególnych gatunków owsa.
Źródło:: Acta Agrobotanica; 2012, 65, 1
0065-0951
2300-357X
Pojawia się w:: Acta Agrobotanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Cacao swollen shoot virus detection and DNA barcoding of its vectors and putative vectors in Theobroma cacao L. by using polymerase chain reaction
Autorzy:: Obok, E.E.
Aikpokpodion, P.O.
Ani, O.C.
Allainguillaume, J.
Wetten, A.
Powiązania:: https://bibliotekanauki.pl/articles/2096411.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Cacao swollen shoot virus
COI – cytochrome c oxidase subunit I
DNA barcoding
Jack Beardsley
mealybug
PCR – polymerase chain reaction
Theobroma cacao
Opis:: Cacao swollen shoot virus (CSSV) is an endemic pathogen causing significant economic losses to cacao (Theobroma cacao L.) production in West Africa. There is limited updated report on the occurrence, spread, genetic diversity and species of CSSV and its mealybug vectors, especially in Nigeria. Nigeria is presently lagging behind in the search for resistance to CSSV and its vectors in T. cacao L. The present study aimed to map and screen for the presence of CSSV and its natural vectors – female mealybugs (Pseudococcidae: Hemiptera) in cacao plantations in Nigeria. Symptomatic and asymptomatic cacao leaves and whole female mealybug samples were collected from major cacao-growing areas in Nigeria – Abia, Akwa Ibom, Cross River, Edo, Ondo and Oyo States. A total of 2568 cacao leaves from 1052 cacao trees were screened with polymerase chain reaction (PCR) using an open reading frame 1 (ORF 1) CSSV-specific primer pair. PCR screening of the mealybug species was performed using the cytochrome c oxidase subunit I (COI) gene. A combination of scanning electron microscopy (SEM) and histology for morphological identification and DNA barcoding enabled to characterise the female mealybug species. The results revealed that CSSV and its mealybug vectors are present in the major cacao-growing areas in Nigeria. Although CSSV and its vectors have been previously reported in Cross River, Ondo and Oyo States, our results present the first documented evidence of CSSV emergence and its mealybug vectors in Abia, Akwa Ibom and Edo States. We also present the first report of Pseudococcus jackbeardsleyi (Gimpel and Miller) mealybug species on cacao in Nigeria. In conclusion, it is pertinent to re-establish coordinated routine survey and monitoring of CSSV and its mealybug vector presence in T. cacao L. in Nigeria.
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 229-244
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Application of DNA markers against illegal logging as a new tool for the Forest Guard Service
Autorzy:: Nowakowska, J.A.
Powiązania:: https://bibliotekanauki.pl/articles/38611.pdf
Data publikacji:: 2011
Wydawca:: Instytut Badawczy Leśnictwa
Tematy:: application
DNA marker
DNA structure
wood
molecular identification
Forest Guard Service
tree species
determination
DNA profile
polymerase chain reaction
Opis:: DNA markers are currently the most precise tool for forest tree species identification and can be used for comparative analyses of plant material. Molecular diagnosis of evidence and reference material is based on comparing the structure of DNA markers duplicated in the PCR reaction and estimation of the DNA profiles obtained in studied wood samples. For this purpose, the microsatellite DNA markers are the most suitable tool because of their high polymorphism and accurate detection of structural changes in the genome. The analysis of tree stump DNA profiles let avoid timely collection of data such as tree age, diameter, height and thickness, although such a piece of information may advantageous in wood identification process. For each examined tree species, i.e. Pinus sylvestris L., Picea abies (L.) Karst., Quercus robur L. and Q. petraea (Matt.) Liebl., Fagus sylvatica L., Betula pendula L., and Alnus glutinosa L., wood identification was possible via the DNA profiles established on a basis of minimum 4 microsatellite nuclear DNA loci, and at least one cytoplasmatic (mitochondrial or chloroplast) DNA marker. Determination of the DNA profiles provided fast and reliable comparison of genetic similarity between material of evidence (wood, needles, leaves, seeds) and material of reference (tree stumps) in the forest. This was done with high probability (approximately 98– 99%).
Źródło:: Folia Forestalia Polonica. Series A . Forestry; 2011, 53, 2
0071-6677
Pojawia się w:: Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:: Biblioteka Nauki

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