Temat: DNA extraction - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Comparison of the utility of five commercial kits for extraction of DNA from Aspergillus fumigatus spores
Autorzy:: Nawrot, Urszula
Wlodarczyk, Katarzyna
Wrobel, Magdalena
Wasik, Anita
Dobosz, Tadeusz
Powiązania:: https://bibliotekanauki.pl/articles/1040325.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: DNA extraction
aspergillosis
Aspergillus
Opis:: The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using Aspergillus-specific primers and SYBR Green I dye or TaqMan probes targeting 28S rRNA gene. All methods allowed the detection of Aspergillus at the lowest tested density of water suspensions of spores (101 cells/ml). The highest DNA yield was obtained using the ZR Fungal/Bacterial DNA kit, YeastStar Genomic DNA kit, and QIAamp DNA Mini kit with mechanical cell disruption. The ZR Fungal/Bacterial DNA and YeastStar kits showed the highest sensitivity in examination of blood samples spiked with Aspergillus (100 % for the detection of 102 spores and 75 % for 101 spores). Recently, the enzymatic method ceased to be recommended for examination of blood samples for Aspergillus, thus ZR Fungal/Bacterial DNA kit and QIAamp DNA Mini kit with mechanical cell disruption could be used for extraction of Aspergillus DNA from clinical samples.
Źródło:: Acta Biochimica Polonica; 2010, 57, 4; 567-571
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Sampling, metadata and DNA extraction - important steps in metagenomic studies
Autorzy:: Felczykowska, Agnieszka
Krajewska, Anna
Zielińska, Sylwia
Łoś, Joanna
Powiązania:: https://bibliotekanauki.pl/articles/1039153.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: sampling
DNA extraction
DNA purification
soil
water
wastewater
metadata
Opis:: Metagenomic studies have become increasingly popular. They allow for the estimation of biodiversity in complex populations. This diversity presents an enormous but largely unexpected genetic and biological pool and can be exploited for the recovery of novel genes, entire metabolic pathways and their products. Generally metagenomic study is a genomic analysis of organisms by direct extraction and cloning of DNA from their natural environment. The most common problems of modern metagenomics are as follows: majority of the microorganisms present in the environment cannot be cultivated by standard techniques, DNA extraction methods are not very effective, isolated DNA is contaminated with various compounds, a choice for a screening method is not obvious.
Źródło:: Acta Biochimica Polonica; 2015, 62, 1; 151-160
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification
Autorzy:: Kobylak, Natalia
Bykowska, Barbara
Nowicki, Roman
Brillowska-Dąbrowska, Anna
Powiązania:: https://bibliotekanauki.pl/articles/1039146.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: fungal infections
dermatophytosis
DNA extraction
real-time PCR
Opis:: Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.
Źródło:: Acta Biochimica Polonica; 2015, 62, 1; 119-122
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:: Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Powiązania:: https://bibliotekanauki.pl/articles/363088.pdf
Data publikacji:: 2007
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification
Opis:: American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Źródło:: Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Genotype characterisation of Blastocystis isolates from Polish patients - preliminary results
Autorzy:: Salamatin, R.
Kaczmarek, A.
Rozej-Bielicka, W.
Cielecka, D.
Janczak, D.
Lewicki, A.
Wesolowska, M.
Mlocicki, D.
Golab, E.
Powiązania:: https://bibliotekanauki.pl/articles/5914.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: genotype
Blastocystis
isolate
DNA extraction
Polish patient
patient
human disease
Źródło:: Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: The unusual Gasteromycetes Lycogalopsis solmsii belongs to the gomphoid-phalloid group
Autorzy:: Demoulin, V.
Cornet, L.
Delbruyere, E.
Baurain, D.
Powiązania:: https://bibliotekanauki.pl/articles/67125.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: Gasteromycetes
Lycogalopsis solmsii
new genus
new species
gomphoid-phalloid group
Lycoperdales
phylogenesis
DNA sequencing
DNA extraction
Opis:: The rare tropical Gasteromycetes Lycogalopsis solmsii has been found twice at thirty years interval in the Singapore Botanic Gardens. From the most recent find a culture could be isolated, which allowed DNA extraction and sequencing of about 2000 bp from the nuclear ribosomal DNA. Comparison to a large sample of Basidiomycetes was only possible for a part of the large ribosomal subunit, but clearly indicated affiliation to the gomphoid-phalloid group, without any relationship to Lycoperdales or Agaricales, as stated in the Dictionary of the Fungi.
Źródło:: Acta Mycologica; 2013, 48, 1
0001-625X
2353-074X
Pojawia się w:: Acta Mycologica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Frequent D-loop polymorphism in mtDNA enables genotyping of 1400-year-old human remains from Merowingian graves
Autorzy:: Zeller, M
Mirghomizadeh, F.
Wehner, H.D.
Blin, N.
Powiązania:: https://bibliotekanauki.pl/articles/2042042.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: X chromosome
ancient remains
amplification technique
Y chromosome
ancient DNA
mtDNA
remains
Merowingian culture
polymorphism
mitochondrial DNA
man
DNA marker
DNA extraction
DNA
Źródło:: Journal of Applied Genetics; 2000, 41, 4; 285-292
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Somatic embryogenesis and genetic uniformity of regenerated cassava plants from low-temperature preserved secondary somatic cotyledons
Autorzy:: Opabode, J.T.
Ajibola, O.V.
Oyelakin, O.O.
Akinyemiju, O.A.
Powiązania:: https://bibliotekanauki.pl/articles/79828.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cassava
Manihot esculenta
somatic embryogenesis
plant regeneration
cotyledon
organogenesis
DNA extraction
RAPD marker
dehydration
low temperature
regeneration
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR
Autorzy:: Trzewik, A.
Nowak, K.J.
Orlikowska, T.
Powiązania:: https://bibliotekanauki.pl/articles/66480.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: simple method
DNA extraction
rhododendron
leaf
plant infection
Phytophthora
polymerase chain reaction
detection
real-time PCR method
Opis:: Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.
Źródło:: Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Enhancement of fungal DNA templates and PCR amplification yield by three types of nanoparticles
Autorzy:: Al-Dhabaan, F.A.
Yousef, H.
Shoala, T.
Shaheen, J.
El Sawi, Y.
Farag, T.
Powiązania:: https://bibliotekanauki.pl/articles/65369.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: plant pathology
detection
identification
plant pathogen
toxigenic fungi
improvement
specificity
efficiency
polymerase chain reaction
Alternaria alternata
DNA extraction
nanoparticle
Rhizoctonia solani
nanobiotechnology
Opis:: Nanodiagonastic methods in plant pathology are used for enhancing detection and identification of different plant pathogens and toxigenic fungi. Improvement of the specificity and efficiency of the polymerase chain reaction (PCR) by using some nanoparticles is emerging as a new area of research. In the current research, silver, zinc, and gold nanoparticles were used to increase the yield of DNA for two plant pathogenic fungi including soil-borne fungus Rhizoctonia solani and toxigenic fungus Alternaria alternata. Gold nanoparticles combined with zinc and silver nanoparticles enhanced both DNA yield and PCR products compared to DNA extraction methods with ALB buffer, sodium dodecyl sulfate, ALBfree from protinase K, ZnNPs and AgNPs. Also, by using ZnNPs and AgNPs the DNA yield was enhanced and the sensitivity of random amplified polymorphic DNA (RAPD) PCR products was increased. Application of nanomaterials in the PCR reaction could increase or decrease the PCR product according to the type of applied nanometal and the type of DNA template. Additions of AuNPs to PCR mix increased both sensitivity and specificity for PCR products of the tested fungi. Thus, the use of these highly stable, commercially available and inexpensive inorganic nano reagents open new opportunities for improving the specificity and sensitivity of PCR amplicon, which is the most important standard method in molecular plant pathology and mycotoxicology.
Źródło:: Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Bacterial community structure influenced by Coscinodiscus sp. in the Vistula river plume
Autorzy:: Ameryk, A.
Hahnke, R.L.
Gromisz, S.
Kownacka, J.
Zalewski, M.
Szymanek, L.
Calkiewicz, J.
Dunalska, J.
Harder, J.
Powiązania:: https://bibliotekanauki.pl/articles/47978.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:: planktonic bacteria
Coscinodiscus
phytoplankton community
primary production
Vistula River
river plume
Baltic Sea
terminal restriction fragment length polymorphism
DNA extraction
16S rRNA gene
dissolved organic matter
Źródło:: Oceanologia; 2014, 56, 4
0078-3234
Pojawia się w:: Oceanologia
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Ascertaining optimal protocols for DNA extraction of different qualities of pike (Esox lucius) tissue samples - a comparison of commonly used solid phase extraction methods
Autorzy:: Eschbach, E.
Powiązania:: https://bibliotekanauki.pl/articles/363074.pdf
Data publikacji:: 2012
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: szczupak
Esox lucius
ekstrakcja DNA
ekstrakcja do fazy stałej
pike
DNA extractions
solid-phase extraction
Opis:: High quality DNA extractions are a prerequisite for genetic studies of a variety of organisms including fish. The current study focused on the applicability of different commercially available solid phase extraction (SPE) methods as the easiest and fastest methods for DNA extraction and their efficiency with different tissue qualities. These were represented by different kinds of pike tissues (fins, muscle, scales) preserved with different methods and stored at different temperatures over different periods of time (0.5 to 10.0 years). All DNA extractions were analysed according to their yield, purity, integrity and functionality in PCR based downstream analysis. Additionally mechanical pre-treatment of poor quality tissues (e.g. old or aged tissues) and efficient ethanol preservation of frozen bulk fin tissue were investigated. All SPE methods yielded functional DNA from very different qualities of pike tissues as shown by PCR analysis of small nuclear (microsatellite) and large mitochondrial (complete D-loop) DNA fragments. DNA from poor quality tissue can be extracted using single column SPE and in some cases mechanical pre-treatment even improved the yield. Good quality tissue as obtained e.g. from commercial fishermen as frozen bulk material is more efficiently preserved by thawing in ethanol at room temperature than at 2-8°C. DNA from these and air dried tissues was very efficiently prepared by applying reverse SPE in 96-well format, allowing for fast processing of a multitude of samples for high throughput analysis.
Źródło:: Environmental Biotechnology; 2012, 8, 1; 7-14
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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