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    Tytuł:
    Plant virus resistance biotechnological approaches : From genes to the CRISPR/Cas gene editing system
    Autorzy:
    Iksat, Nurgul
    Masalimov, Zhaksylyk
    Omarov, Rustem
    Powiązania:
    https://bibliotekanauki.pl/articles/27312654.pdf
    Data publikacji:
    2023
    Wydawca:
    Instytut Technologiczno-Przyrodniczy
    Tematy:
    CRISPR/Cas9
    CRISPR/Cas13
    plant disease resistance
    plant immunity
    plant virus
    Opis:
    Plant viruses cause crop losses in agronomically and economically important crops, making global food security a challenge. Although traditional plant breeding has been effective in controlling plant viral diseases, it is unlikely to solve the problems associated with the frequent emergence of new and more virulent virus species or strains. As a result, there is an urgent need to develop alternative virus control strategies that can be used to more easily contain viral diseases. A better understanding of plant defence mechanisms will open up new avenues for research into plant-pathogen interactions and the development of broad-spectrum virus resistance. T he scientific literature was evaluated and structured in this review, and the results of the reliability of the methods of analysis used were filtered. As a result, we described the molecular mechanisms by which viruses interact with host plant cells. To develop an effective strategy for the control of plant pathogens with a significant intensity on the agricultural market, clear and standardised recommendations are required. The current review will provide key insights into the molecular underpinnings underlying the coordination of plant disease resistance, such as main classes of resistance genes, RNA interference, and the RNA-mediated adaptive immune system of bacteria and archaea - clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated Cas proteins - CRISPR/Cas. Future issues related to resistance to plant viral diseases will largely depend on integrated research to transfer fundamental knowledge to applied problems, bridging the gap between laboratory and field work.
    Źródło:
    Journal of Water and Land Development; 2023, 57; 147--158
    1429-7426
    2083-4535
    Pojawia się w:
    Journal of Water and Land Development
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Opracowanie i wykorzystanie metod biotechnologicznych do skrócenia cyklu hodowlanego pszenżyta oraz do poprawy efektywności selekcji — miejscowo-specyficzna mutageneza z wykorzystaniem miejscowo-specyficznych nukleaz
    Development and application of methods for the shortening of triticale breeding cycle and for the effective selection — site-specific mutagenesis using programmable nucleases
    Autorzy:
    Linkiewicz, Anna
    Michalski, Krzysztof
    Powiązania:
    https://bibliotekanauki.pl/articles/2199532.pdf
    Data publikacji:
    2019-11-30
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    Crispr-Cas9
    edytowanie genomu
    mutageneza miejscowo-specyficzna
    pszenżyto
    TALEN
    Źródło:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2019, 286; 81-82
    0373-7837
    2657-8913
    Pojawia się w:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CRISPR/Cas9 in plant biotechnology: applications and challenges
    Autorzy:
    Gan, W.C.
    Ling, A.P.K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096248.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    CRISPR/Cas9
    crop improvement
    gene editing
    plant biotechnology
    regulations
    Opis:
    The application of plant biotechnology to enhance beneficial traits in crops is now indispensable because of food insecurity due to increasing global population and climate change. The recent biotechnological development of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated system 9 (Cas9) allows for a more simple and precise method of gene editing, which is now preferred compared to Zinc Finger Nucleases (ZFNs) and Transcription Activator-like Effector Nucleases (TALENs). In this review, recent progress in utilizing CRISPR/Cas9-mediated gene editing in plants to enhance certain traits in beneficial crops, including rice, soybean, and oilseed rape, is discussed. In addition, novel methods of applying the CRISPR/Cas9 system in live cell imaging are also extensively reviewed. Despite all the applications, the existing delivery methods of CRISPR/Cas9 fail to provide consistent results and are inefficient for in planta transformation. Hence, research should be focused on improving current delivery methods or developing novel ones to facilitate CRISPR/Cas9-based gene editing studies. Strict regulations on the sale and commercial growth of gene-edited crops have restricted more efforts in applying CRISPR/Cas9 technology in plant species. Therefore, a shift in public viewpoint toward gene editing would help to propel scientific progress rapidly.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 1; 81-93
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The genetics of the XXI century
    Autorzy:
    Węgleński, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/704235.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    genetic engineering
    DNA sequencing
    CRISPR-Cas technique
    GMO
    protests against GMO
    Opis:
    In recent years, two developments revolutionized the molecular genetics. The first one is an enormously improved technique of the DNA sequencing. It is now possible to obtain in couple of hours and on the low cost, the full sequence of the human genome. The second one is the invention of the CRISPR-Cas technique, which allows the precise manipulations of genomes of living organisms. This technique was already successfully applied to “repair” human genes responsible for hereditary diseases. To some astonishment, applications of genetic engineering to humans does not bring protests of general public, while similar manipulations pf plants genomes are considered an dangerous. In Poland and in some other countries, parliaments introduced an extremely restrictive laws, which in practice makes cultivation of GM plants outside laboratories impossible. This is caused by the ignorance of the general public and by opposition of catholic church which considers genetic modifications of all organisms as contradictory with Gods will. The most common modification of plants is an introduction into their genomes of the single gene from the bacterium Bacillus thuringensis, what make the plant resistant to harmful insects. According to WHO, neither the manipulation mentioned above, nor manipulations of the other kind, do not pose any danger to humans.
    Źródło:
    Nauka; 2020, 1
    1231-8515
    Pojawia się w:
    Nauka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthetic chromosome - the first element for synthetic life
    Autorzy:
    Noszka, Mateusz
    Kilisch, Julia
    Powiązania:
    https://bibliotekanauki.pl/articles/1165623.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    CRISPR/Cas9
    DNA assembly
    synthetic biology
    synthetic chromosome
    λ Red
    Opis:
    Synthetic biology is a scientific area, that link together domains such as: biotechnology, microbiology, system biology, genetic engineering and bioinformatics. The main goal of synthetic biology is to constructing and designing artificial biological systems or re-design of existing biological systems for useful purposes - synthesizing for knowledge and synthesizing for products. The main structure on which synthetic biologists focus is the chromosome. Thanks to the development of modern genome editing techniques such as CRISPR/Cas9, or DNA assembly methods in recent years, there are more and more possibilities of creating synthetic chromosomes. The current achievements give hope for the construction of a fully synthetic organism into the future, as well as open up new possibilities related to the synthesis of new products. The following paper presents actual knowledge about synthetic chromosome including the topic of genome editing method and potential applications of synthetic biology.
    Źródło:
    World Scientific News; 2018, 107; 185-195
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Type III CRISPR complexes from Thermus thermophilus
    Autorzy:
    Szychowska, Marta
    Siwek, Wojciech
    Pawolski, Damian
    Kazrani, Asgar
    Pyrc, Krzysztof
    Bochtler, Matthias
    Powiązania:
    https://bibliotekanauki.pl/articles/1038832.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CRISPR-Cas
    endonuclease
    Csm
    Cmr
    Thermus thermophilus
    crRNA
    mass spectrometry (MS)
    chromatography
    Opis:
    Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology. We have selected two highly expressed CRISPR spacers, crRNA 2.1 and crRNA 2.2, and have enriched endogenous T. thermophilus proteins that co-purify with these crRNAs. Mass spectroscopy indicates that the chromatography protocol enriches predominantly Csm complex subunits, but also Cmr subunits. After several chromatographic steps, size exclusion chromatography indicated a molecular mass of the crRNA associated complex of 265±69 kDa. In agreement with earlier work, crRNAs of different lengths (containing the selected spacers) were observed. Most of these were completely lost when several T. thermophilus csm genes were ablated.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 2; 377-386
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pharmacological versus genetic inhibition of heme oxygenase-1 - the comparison of metalloporphyrins, shRNA and CRISPR/Cas9 system
    Autorzy:
    Mucha, Olga
    Podkalicka, Paulina
    Czarnek, Maria
    Biela, Anna
    Mieczkowski, Mateusz
    Kachamakova-Trojanowska, Neli
    Stepniewski, Jacek
    Jozkowicz, Alicja
    Dulak, Jozef
    Loboda, Agnieszka
    Powiązania:
    https://bibliotekanauki.pl/articles/1038402.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CRISPR/Cas9
    shRNA
    inhibitors
    heme oxygenase-1
    HO-1
    off-target
    Opis:
    Inhibition of heme oxygenase-1 (HO-1, encoded by HMOX1), a cytoprotective, anti-apoptotic and anti-inflammatory enzyme, may serve as a valuable therapy in various pathophysiological processes, including tumorigenesis. We compared the effect of chemical inhibitors - metalloporphyrins, with genetic tools - shRNA and CRISPR/Cas9 systems, to knock-down (KD)/knock-out (KO) HO-1 expression/activity. 293T cells were incubated with metalloporphyrins, tin and zinc protoporphyrins (SnPPIX and ZnPPIX, respectively) or were either transduced with lentiviral vectors encoding different shRNA sequences against HO-1 or were modified by CRISPR/Cas9 system targeting HMOX1. Metalloporphyrins decreased HO activity but concomitantly strongly induced HO-1 mRNA and protein in 293T cells. On the other hand, only slight basal HO-1 inhibition in shRNA KD 293T cell lines was confirmed on mRNA and protein level with no significant effect on enzyme activity. Nevertheless, silencing effect was much stronger when CRISPR/Cas9-mediated knock-out was performed. Most of the clones harboring mutations within HMOX1 locus did not express HO-1 protein and failed to increase bilirubin concentration after hemin stimulation. Furthermore, CRISPR/Cas9-mediated HO-1 depletion decreased 293T viability, growth, clonogenic potential and increased sensitivity to H2O2 treatment. In summary, we have shown that not all technologies can be used for inhibition of HO activity in vitro with the same efficiency. In our hands, the most potent and comprehensible results can be obtained using genetic tools, especially CRISPR/Cas9 approach.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 2; 277-286
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Różne strategie osiągania pamięci immunologicznej
    Different strategies for attaining immune memory
    Autorzy:
    Wojda, Iwona
    Powiązania:
    https://bibliotekanauki.pl/articles/1034027.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Tematy:
    CRISPR/Cas
    DSCAM receptors
    immune memory
    immunoglobulins
    RAG,VLR receptors
    immunoglobuliny
    pamięć immunologiczna
    RAG
    receptory DSCAM
    receptory VLR
    Opis:
    Odporność nabyta inaczej adaptacyjna (swoista) rozwinęła się w ewolucji bardzo późno, bo dopiero u kręgowców żuchwowych. Oparta jest ona na limfocytach T i B oraz syntezie różnorodnych receptorów i przeciwciał. Okazuje się jednak, że u bezkręgowców, których odporność oparta jest jedynie na mechanizmach wrodzonych, obserwuje się swego rodzaju pamięć immunologiczną. Co więcej, nawet organizmy jednokomórkowe jak bakterie czy archeony wykazują cechy "pamięci immunologicznej". W artykule opisano różne strategie "zapamiętywania" infekcji: mechanizm CRISPR/Cas u bakterii, receptory DSCAM i inne formy piętnowania układu immunologicznego owadów oraz zmienność receptorów bogatych w leucynę (LRR) u bezżuchwowców. Przedstawiono także jak doszło do nabycia możliwości syntezy różnorodnych przeciwciał oraz receptorów limfocytów. Opisane mechanizmy opierają się na włączaniu obcego materiału genetycznego do genomu gospodarza, mechanizmie konwersji genów, alternatywnego składania transkryptów oraz somatycznej rearanżacji DNA.
    Acquired immunity (adaptive, specific) developed late in evolution - in jawed vertebrates. It is based on T and B lymphocytes and a diversity of antibodies. It turns out, however, that in invertebrates, which posses only innate mechanisms there is a kind of immune memory. Moreover, even single-cell organisms such as bacteria or archaea exhibit features of immunological memory. This article describes the various strategies used to achieve a kind of rememmbrnace of infection: a CRISPR/Cas system in bacteria, diveristy of DSCAM receptors and other forms of immune priming in insects, leucine-rich receptors in jawless vertebrates. It also describes how it came to acquire the possibility of synthesis of various forms of antibodies and lymphocyte receptors by jawed vertebrates. The described mechanisms are based on the incorporation of foreign genetic material into host genome, the gene conversion mechanisms, alternative splicing and finally, somatic rearrangements of DNA.
    Źródło:
    Kosmos; 2017, 66, 4; 721-732
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optymalizacja metod detekcji mutacji w genie Nud indukowanej przez technologię CRISPR/Cas9 w jęczmieniu zwyczajnym (Hordeum vulgare L.)
    Optimization of mutation detection methods in the NUD gene induced by CRISPR / CAS9 technology in barley (Hordeum vulgare L.)
    Autorzy:
    Przyborowski, Mateusz
    Gasparis, Sebastian
    Orczyk, Wacław
    Nadolska-Orczyk, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/2199916.pdf
    Data publikacji:
    2019-11-30
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    CRISPR / Cas9
    edytowanie genomu
    gen Nud
    jęczmień zwyczajny
    sondy molekularne
    genome editing
    Nud gene
    barley
    molecular probes
    Opis:
    Celem pracy był wybór optymalnej metody detekcji mutacji w genie Nud indukowanej z wykorzystaniem technologii CRISPR/Cas9 w jęczmieniu zwyczajnym. Testowano cztery powszechnie wykorzystywane techniki genotypowania: polimorfizm długości fragmentów restrykcyjnych (RFLP), trawienie enzymatyczne przy użyciu endonukleazy I z bakteriofaga T7, wysokorozdzielczą krzywą topnienia produktu reakcji łańcuchowej polimerazy (HRM-PCR) oraz sondy molekularne. W toku prowadzonych prac stwierdzono, że najkorzystniejszym sposobem wykrywania mutacji indukowanej techniką CRISPR/Cas9 jest metoda oparta o sondy molekularne. Daje ona jednoznaczne i powtarzalne wyniki ale jednocześnie wymaga użycia zawansowanej aparatury.
    The aim of the study was to select the optimal method for mutation detection in the Nud gene induced by using the CRISPR / Cas9 technology in barley. Four commonly used genotyping techniques were tested: restriction fragment length polymorphism (RFLP), enzymatic digestion using endonuclease I from T7 bacteriophage, high resolution melting curve of polymerase chain reaction product (HRM-PCR) and molecular probes. Findings in the current study suggest that the most favorable way for detecting mutations induced by the CRISPR / Cas9 technique is a method based on molecular probes. It enables to receive clear and repeatable results but at the same time requires the use of advanced equipment.
    Źródło:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2019, 287; 33-34
    0373-7837
    2657-8913
    Pojawia się w:
    Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genome editing of crop plants. Facts, no myths
    Autorzy:
    Orczyk, Wacław
    Powiązania:
    https://bibliotekanauki.pl/articles/704588.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conventional mutagenesis
    Court of Justice C-528/16
    CRISPR/Cas9 cultivated plants
    domestication
    genome editing
    orphan crops
    pathogen resistance
    yield and yield quality
    Opis:
    Article published in Science, 2012 by Jennifer A. Doudna, Emmanuelle Charpentier and their team presented a novel tool named as CRISPR/Cas9.  The original CRISPR/Cas9 tool and the whole system developed from it since then allow making precise changes in the nucleotide sequence in the defined locus of the genome. The article presents the already known as well the potential future applications of the system for improvement of cultivated plants. The separate section is devoted to present the background of the Court of Justice decision C-528/16. Discussed are the far reaching negative consequences of this, based not on the merit decision, for the future of European green biotechnology.
    Źródło:
    Nauka; 2019, 4
    1231-8515
    Pojawia się w:
    Nauka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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